BDH1 (NM_004051) Human Untagged Clone

CAT#: SC103002

BDH1 (untagged)-Human 3-hydroxybutyrate dehydrogenase, type 1 (BDH1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BDH1
• Synonyms: BDH; SDR9C1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004051 edited
  ATGCTGGCCACCCGCCTCTCCAGACCCCTGTCACGGCTCCCAGGAAAAACCCTAAGTGCC
  TGTGATAGAGAAAATGGAGCAAGACGCCCACTATTGCTTGGTTCTACTTCCTTTATCCCG
  ATTGGCCGTCGGACTTATGCCAGTGCGGCGGAGCCGGTTGGCAGCAAAGCTGTCCTGGTC
  ACAGGCTGTGACTCTGGATTTGGGTTCTCATTGGCCAAGCATCTGCATTCAAAAGGCTTC
  CTTGTGTTTGTTGGCTGCTTGATGAAGGACAAAGGCCATGATGGGGTCAAGGAGCTGGAC
  AGCCTAAACAGTGACCGATTGAGAACCGTCCAGCTCAATGTCTGCAGCAGCGAAGAGGTG
  GAGAAAGTGGTGGAGATTGTCCGCTCGAGCCTGAAGGACCCTGAGAAAGGCATGTGGGGC
  CTCGTTAACAATGCCGGCATCTCAACGTTCGGGGAGGTGGAGTTCACCAGCCTGGAGACC
  TACAAGCAGGTGGCAGAAGTGAACCTTTGGGGCACAGTGCGGATGACGAAATCCTTTCTC
  CCCCTCATCCGAAGGGCCAAAGGCCGCGTCGTCAATATCAGCAGCATGCTGGGCCGCATG
  GCCAACCCGGCCCGCTCCCCGTACTGCATCACCAAGTTCGGGGTAGAGGCTTTCTCGGAC
  TGCCTGCGCTATGAGATGTACCCCCTGGGCGTGAAGGTCAGCGTGGTGGAGCCCGGCAAC
  TTCATCGCTGCCACCAGCCTTTACAGCCCTGAGAGCATTCAGGCCATCGCCAAGAAGATG
  TGGGAGGAGCTGCCTGAGGTCGTGCGCAAGGACTACGGCAAGAAGTACTTTGATGAAAAG
  ATCGCCAAGATGGAGACCTACTGCAGCAGTGGCTCCACAGACACGTCCCCTGTCATCGAT
  GCTGTCACACACGCCCTGACCGCCACCACCCCCTACACCCGCTACCACCCCATGGACTAC
  TACTGGTGGCTGCGAATGCAGATCATGACCCACTTGCCTGGAGCCATCTCCGACATGATC
  TACATCCGCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004051 unedited
  CCCTTTTGTATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGGCGGAGGCCG
  CAGGCCGCAGGAGTGCTGGTGGAGGGGCTTCCAGAAAGACCCTGCGGCAGCGCCCCCCCT
  CGGCTCTCCCCGCAGGAGAGCGGGCACCTGCGCGGCGCCGGGTGAAGGCGAGAGCCTCGG
  CGAGCCCTCTGCAGCGGAGCCCCCTGCCCATTTGGTTTTGGCCAGTCAGCCTGGAAATCT
  GCCTGTAGTCAGCTGATGCAGAGTCACCCTGCAGTTCCTACTTTGAGGGGTTGCAGAAGC
  ACATATTTCACTATGGAGGGCCGCCTGCAGGCCTCGCGCTGACAGGATCTTTTGTGACGC
  TGCAAATGTCCTGAACCACCGGGAGGAACTGGGCCATTCTAACACCCGTTGCTACCATGC
  TGGCCACCCGCCTCTCCAGACCCCTGTCACGGCTCCCAGGAAAAACCCTAAGTGCCTGTG
  ATAGAGAAAATGGAGCAAGACGCCCACTATTGCTTGGTTCTACTTCCTTTATCCCGATTG
  GCCGTCGGACTTATGCCAGTGCGGCGGAGCCGGTTGGCAGCAAAGCTGTCCTGGTCACAG
  GCTGTGACTCTGGATTTGGGTTCTCATTGGCCAAGCATCTGCATTCAAAAGGCTTCCTTG
  CGTTTGCTGGCTGCTCGATGAACGACAACAGGCCATGATGGTGTCAAGGAGCTGGACAGC
  CTAAACAGTGACCCGATGAGAACCGTCCAGCTCAATGTCTGCAGCACCGAAGAAGTGGAG
  AAAGTGGTGGAGATTGTCCGCTCGAGCCTGAAGACCCTGAGAAAGCATGTGGGGCCCTGN
  TNACCATGCCCGCATTTCAACGTTCGGGGAGGTGGAGTTCACACGCCTGGAACCTACAAG
  CACGTGCCATAATGAACCTTTGGGGCCCAGTGCGGATGACCAAATCCTTTTTCCCGTTAT
  ACAAAGGCCAAAGACGNCGTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004051 unedited
  AAGGATGTTTTTCTTAACAATCTCTGTAAGAGAATTATCTCCGGTAGAGAAGATTCACCA
  TGTTTGCCCTGAGCTTTAGAGGCCTATGCCTGCCACTCCACACCCTGTTTGTGAAGGCCC
  AAGTCACTCACTATGCAAAGAACTCATTCCCTCTAGTTAGTGTTAAAACCAGTTATGGGT
  CTTCCTGGCTTGGTGGATAATCCACACCTGGATAATCAAGAGTTGACTATATGGGTTCCT
  CCATTGCCTCCGCTTCCACCAGGGATCCCTGACAGAGGCCACATTGAGACTCTTCACCGG
  ATGTATATCATGTCAGAGATGGCTCCATGCAAGTGGGTCATGATCTGCATTCGCAGCCTC
  CAATACTAGTCCATGGGGTGGTAACGAGTGTATGGGGTGGTGGCGGTTCACGGCGTGTGT
  GACAGCATCCATGACCTGGGACCTGTCTGTGGAGCCACTGCTGAAGTAGGTCTCCATCTT
  GCACATCTTTTCATCAAAGTACTTCTTGCCGTGGTCCTTGGGCGCAGACCTCGGCTGCTC
  CGTACACGTCTTCTTAGCTATGGCCTGAATGCTCTCACGGGCTGTAAGGCTGGTGGCAGC
  GATTGAAGTAGGAGGATACATCAAACCTGCCTTGCCGCCCATGGGGTGCGTCTCATTCAG
  CAGGCTGTCCTAGAAAGCGTCTGACACAAACTTGGTGATTCTCTACAGCGAACCGGCCCA
  GTTTGCCCATGCTGAACCACAATGCTGGTGATGTTGATGAAAACCCCTTTCGCCCTTCGG
  AATTAGGGGAAGAAGGATATTGATCTTCACACTGAGCTCCCAACGTTACTTTTGCAACCT
  GGTTTATGTTCTCCGGCAGGGGAAACCCCCTCCCCCCAATTTTATAACCGGCTTGTTAAT
  AAACCCAATGAAGTTCCCAGGATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004051
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004051.3, NP_004042.1
• RefSeq Size: 1621 bp
• RefSeq ORF: 1032 bp
• Locus ID: 622
• Cytogenetics: 3q29
• Domains: adh_short
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies
• Gene Summary: 'This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, and 3 encode the same protein.