BDH1 (NM_004051) Human Untagged Clone

CAT#: SC103002

BDH1 (untagged)-Human 3-hydroxybutyrate dehydrogenase, type 1 (BDH1), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_004051" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol BDH1
Synonyms BDH; SDR9C1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_004051 edited
ATGCTGGCCACCCGCCTCTCCAGACCCCTGTCACGGCTCCCAGGAAAAACCCTAAGTGCC
TGTGATAGAGAAAATGGAGCAAGACGCCCACTATTGCTTGGTTCTACTTCCTTTATCCCG
ATTGGCCGTCGGACTTATGCCAGTGCGGCGGAGCCGGTTGGCAGCAAAGCTGTCCTGGTC
ACAGGCTGTGACTCTGGATTTGGGTTCTCATTGGCCAAGCATCTGCATTCAAAAGGCTTC
CTTGTGTTTGTTGGCTGCTTGATGAAGGACAAAGGCCATGATGGGGTCAAGGAGCTGGAC
AGCCTAAACAGTGACCGATTGAGAACCGTCCAGCTCAATGTCTGCAGCAGCGAAGAGGTG
GAGAAAGTGGTGGAGATTGTCCGCTCGAGCCTGAAGGACCCTGAGAAAGGCATGTGGGGC
CTCGTTAACAATGCCGGCATCTCAACGTTCGGGGAGGTGGAGTTCACCAGCCTGGAGACC
TACAAGCAGGTGGCAGAAGTGAACCTTTGGGGCACAGTGCGGATGACGAAATCCTTTCTC
CCCCTCATCCGAAGGGCCAAAGGCCGCGTCGTCAATATCAGCAGCATGCTGGGCCGCATG
GCCAACCCGGCCCGCTCCCCGTACTGCATCACCAAGTTCGGGGTAGAGGCTTTCTCGGAC
TGCCTGCGCTATGAGATGTACCCCCTGGGCGTGAAGGTCAGCGTGGTGGAGCCCGGCAAC
TTCATCGCTGCCACCAGCCTTTACAGCCCTGAGAGCATTCAGGCCATCGCCAAGAAGATG
TGGGAGGAGCTGCCTGAGGTCGTGCGCAAGGACTACGGCAAGAAGTACTTTGATGAAAAG
ATCGCCAAGATGGAGACCTACTGCAGCAGTGGCTCCACAGACACGTCCCCTGTCATCGAT
GCTGTCACACACGCCCTGACCGCCACCACCCCCTACACCCGCTACCACCCCATGGACTAC
TACTGGTGGCTGCGAATGCAGATCATGACCCACTTGCCTGGAGCCATCTCCGACATGATC
TACATCCGCTGA
>OriGene 5' read for NM_004051 unedited
CCCTTTTGTATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGGCGGAGGCCG
CAGGCCGCAGGAGTGCTGGTGGAGGGGCTTCCAGAAAGACCCTGCGGCAGCGCCCCCCCT
CGGCTCTCCCCGCAGGAGAGCGGGCACCTGCGCGGCGCCGGGTGAAGGCGAGAGCCTCGG
CGAGCCCTCTGCAGCGGAGCCCCCTGCCCATTTGGTTTTGGCCAGTCAGCCTGGAAATCT
GCCTGTAGTCAGCTGATGCAGAGTCACCCTGCAGTTCCTACTTTGAGGGGTTGCAGAAGC
ACATATTTCACTATGGAGGGCCGCCTGCAGGCCTCGCGCTGACAGGATCTTTTGTGACGC
TGCAAATGTCCTGAACCACCGGGAGGAACTGGGCCATTCTAACACCCGTTGCTACCATGC
TGGCCACCCGCCTCTCCAGACCCCTGTCACGGCTCCCAGGAAAAACCCTAAGTGCCTGTG
ATAGAGAAAATGGAGCAAGACGCCCACTATTGCTTGGTTCTACTTCCTTTATCCCGATTG
GCCGTCGGACTTATGCCAGTGCGGCGGAGCCGGTTGGCAGCAAAGCTGTCCTGGTCACAG
GCTGTGACTCTGGATTTGGGTTCTCATTGGCCAAGCATCTGCATTCAAAAGGCTTCCTTG
CGTTTGCTGGCTGCTCGATGAACGACAACAGGCCATGATGGTGTCAAGGAGCTGGACAGC
CTAAACAGTGACCCGATGAGAACCGTCCAGCTCAATGTCTGCAGCACCGAAGAAGTGGAG
AAAGTGGTGGAGATTGTCCGCTCGAGCCTGAAGACCCTGAGAAAGCATGTGGGGCCCTGN
TNACCATGCCCGCATTTCAACGTTCGGGGAGGTGGAGTTCACACGCCTGGAACCTACAAG
CACGTGCCATAATGAACCTTTGGGGCCCAGTGCGGATGACCAAATCCTTTTTCCCGTTAT
ACAAAGGCCAAAGACGNCGTN
>OriGene 3' read for NM_004051 unedited
AAGGATGTTTTTCTTAACAATCTCTGTAAGAGAATTATCTCCGGTAGAGAAGATTCACCA
TGTTTGCCCTGAGCTTTAGAGGCCTATGCCTGCCACTCCACACCCTGTTTGTGAAGGCCC
AAGTCACTCACTATGCAAAGAACTCATTCCCTCTAGTTAGTGTTAAAACCAGTTATGGGT
CTTCCTGGCTTGGTGGATAATCCACACCTGGATAATCAAGAGTTGACTATATGGGTTCCT
CCATTGCCTCCGCTTCCACCAGGGATCCCTGACAGAGGCCACATTGAGACTCTTCACCGG
ATGTATATCATGTCAGAGATGGCTCCATGCAAGTGGGTCATGATCTGCATTCGCAGCCTC
CAATACTAGTCCATGGGGTGGTAACGAGTGTATGGGGTGGTGGCGGTTCACGGCGTGTGT
GACAGCATCCATGACCTGGGACCTGTCTGTGGAGCCACTGCTGAAGTAGGTCTCCATCTT
GCACATCTTTTCATCAAAGTACTTCTTGCCGTGGTCCTTGGGCGCAGACCTCGGCTGCTC
CGTACACGTCTTCTTAGCTATGGCCTGAATGCTCTCACGGGCTGTAAGGCTGGTGGCAGC
GATTGAAGTAGGAGGATACATCAAACCTGCCTTGCCGCCCATGGGGTGCGTCTCATTCAG
CAGGCTGTCCTAGAAAGCGTCTGACACAAACTTGGTGATTCTCTACAGCGAACCGGCCCA
GTTTGCCCATGCTGAACCACAATGCTGGTGATGTTGATGAAAACCCCTTTCGCCCTTCGG
AATTAGGGGAAGAAGGATATTGATCTTCACACTGAGCTCCCAACGTTACTTTTGCAACCT
GGTTTATGTTCTCCGGCAGGGGAAACCCCCTCCCCCCAATTTTATAACCGGCTTGTTAAT
AAACCCAATGAAGTTCCCAGGATT
Restriction Sites NotI-NotI     
ACCN NM_004051
Insert Size 1700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004051.3, NP_004042.1
RefSeq Size 1621 bp
RefSeq ORF 1032 bp
Locus ID 622
Cytogenetics 3q29
Domains adh_short
Protein Families Druggable Genome
Protein Pathways Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies
Gene Summary 'This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, and 3 encode the same protein.

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