CYP1B1 (NM_000104) Human Untagged Clone

CAT#: SC103335

CYP1B1 (untagged)-Human cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP1B1
• Synonyms: ASGD6; CP1B; CYPIB1; GLC3A; P4501B1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC103335 sequence for NM_000104 edited (data generated by NextGen Sequencing)
  ATGGGCACCAGCCTCAGCCCGAACGACCCTTGGCCGCTAAACCCGCTGTCCATCCAGCAG
  ACCACGCTCCTGCTACTCCTGTCGGTGCTGGCCACTGTGCATGTGGGCCAGCGGCTGCTG
  AGGCAACGGAGGCGGCAGCTCGGGTCCGCGCCCCCGGGCCCGTTTGCGTGGCCACTGATC
  GGAAACGCGGCGGCGGTGGGCCAGGCGGCTCACCTCTCGTTCGCTCGCCTGGCGCGGCGC
  TACGGCGACGTTTTCCAGATCCGCCTGGGCAGCTGCCCCATAGTGGTGCTGAATGGCGAG
  CGCGCCATCCACCAGGCCCTGGTGCAGCAGGGCTCGGCCTTCGCCGACCGGCCGTCCTTC
  GCCTCCTTCCGTGTGGTGTCCGGCGGCCGCAGCATGGCTTTCGGCCACTACTCGGAGCAC
  TGGAAGGTGCAGCGGCGCGCAGCCCACAGCATGATGCGCAACTTCTTCACGCGCCAGCCG
  CGCAGCCGCCAAGTCCTCGAGGGCCACGTGCTGAGCGAGGCGCGCGAGCTGGTGGCGCTG
  CTGGTGCGCGGCAGCGCGGACGGCGCCTTCCTCGACCCGAGGCCGCTGACCGTCGTGGCC
  GTGGCCAACGTCATGAGTGCCGTGTGTTTCGGCTGCCGCTACAGCCACGACGACCCCGAG
  TTCCGTGAGCTGCTCAGCCACAACGAAGAGTTCGGGCGCACGGTGGGCGCGGGCAGCCTG
  GTGGACGTGATGCCCTGGCTGCAGTACTTCCCCAACCCGGTGCGCACCGTTTTCCGCGAA
  TTCGAGCAGCTCAACCGCAACTTCAGCAACTTCATCCTGGACAAGTTCTTGAGGCACTGC
  GAAAGCCTTCGGCCCGGGGCCGCCCCCCGCGACATGATGGACGCCTTTATCCTCTCTGCG
  GAAAAGAAGGCGGCCGGGGACTCGCACGGTGGTGGCGCGCGGCTGGATTTGGAGAACGTA
  CCGGCCACTATCACTGACATCTTCGGCGCCAGCCAGGACACCCTGTCCACCGCGCTGCAG
  TGGCTGCTCCTCCTCTTCACCAGGTATCCTGATGTGCAGACTCGAGTGCAGGCAGAATTG
  GATCAGGTCGTGGGGAGGGACCGTCTGCCTTGTATGGGTGACCAGCCCAACCTGCCCTAT
  GTCCTGGCCTTCCTTTATGAAGCCATGCGCTTCTCCAGCTTTGTGCCTGTCACTATTCCT
  CATGCCACCACTGCCAACACCTCTGTCTTGGGCTACCACATTCCCAAGGACACTGTGGTT
  TTTGTCAACCAGTGGTCTGTGAATCATGACCCACTGAAGTGGCCTAACCCGGAGAACTTT
  GATCCAGCTCGATTCTTGGACAAGGACGGCCTCATCAACAAGGACCTGACCAGCAGAGTG
  ATGATTTTTTCAGTGGGCAAAAGGCGGTGCATTGGCGAAGAACTTTCTAAGATGCAGCTT
  TTTCTCTTCATCTCCATCCTGGCTCACCAGTGCGATTTCAGGGCCAACCCAAATGAGCCT
  GCGAAAATGAATTTCAGTTATGGTCTAACCATTAAACCCAAGTCATTTAAAGTCAATGTC
  ACTCTCAGAGAGTCCATGGAGCTCCTTGATAGTGCTGTCCAAAATTTACAAGCCAAGGAA
  ACTTGCCAATAA
  
  Clone variation with respect to NM_000104.3
  142 c=>g;355 g=>t;1347 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000104 unedited
  CTCCGTTTGTAACCGACTTTCTATAGGCGGCCGACGAATTCGCACGAGGTGGGAGCGAGC
  GCTTCTGCGACTCCGTTGTGAGAGCCGCAGGGCATGGGAATTGACGCCACTCACCGACCC
  CCAGTCTCAATCTCAACGCTGTGAGGAAACCTCGACTTTGCCAGGTCCCCAAGGGCAGCG
  GGGCTCGGCGAGCGAGGCACCCTTCTCCGTCCCCATCCCAATCCAAGCGCTCCTGGCACT
  GACGACGCCAAGAGACTCGAGTGGGAGTTAAAGCTTCCAGTGAGGGCAGCAGGTGTCCAG
  GCCGGGCCTGCGGGTTCCTGTTGACGTCTTGCCCTATGCAAAGGTCCCAGTTCCTTCTCG
  GAGCCGGCTGTCCCGCGCCACTGGAAACCGCACCTCCCCGCAGCATGGGCACCAGCCTCA
  GCCCGAACGACCCTTGGCCGCTAAACCCGCTGTCCATCCAGCAGACCACGCTCCTGCTAC
  TCCTGTCGGTGCTGGCCACTGTGCATGTGGGCCAGCGGCTGCTGAGGCAACGGATGCGGC
  AGCTCGGGTCCGCGCCCCCGGGCCCGTTTGCGTGGCCACTGATCGGAAACGCGGCGGCGG
  TGGGCCAGGCGGCTCACCTCTCGTTCGCTCGCCTGGCGCGGCGCTACGGCGACGTTTTCC
  AGATCCGCCTGGGCAGCTGCCCCATAGTGGTGCTGAATGGCGAGCGCGCCATCCACCATG
  CCCTGGTGCAGCATGGCTCGGCCTTCGCCGACCGGCCGTCCTTCGCCTCCTTCCGTGTGG
  TGTCCGGCAGCCGCAGCATGGCTTTCGGCCACTACTCNGAGCACTGGAANGTGCAGCGGC
  GCGCATCCCACACATGATGCGCAACTTCTTCACGCGCCAGCCGCGCATCCGCAAGTCCTC
  GAGGCCACGTGCTGACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000104 unedited
  CGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGCTGATAATTCAAATTCTGTA
  CTTTGGCAAGCAAAAGAGGTACAACATCACCTTGGAGTTTTACAATTTAATAATGCATAC
  TTTAAAATTCATGATAAATCATGGAACCCCACTATACTCACTAATTCAACTATTGATTTC
  TTTTTGAGCAACTGACTTTATGATTTATCCTTAAAAGTAAGGAAGTATACCAGAAGGCAA
  AAGGATAAATCCACCCATAAATACTAGAGTCTAAGTTATTTTCCTGAAAAGGTGAAAAGG
  GAATTTCTGGTCTTTATCTTTTCCCTTATCACCTTCAATCACACACTTTACACTGAAATT
  ACTTGTCTCTGTCTCTTCAACAATATACTCTGAAACTTTGTTTAATATACTTTTTGTCTA
  TTTCTGCATATGGGAGACCAATCTTGGATTCCCACCAAAAATGGCCTGGTTACCAAAATA
  CTTCTTCATTCAGTAGTTTGGTGTAAGTGTTTGGGTGTATGTGTCTATATGTGCATTTTA
  ACTCCAAAGAATGCTACCTTCAGAAATAACCAAGATGCAGTATGTATATAATAATTCATT
  GGGCCCTTTAAGTCTTTGACTCAAAAAAAATCTCCCAGAAGCTCCTGCATAGCCCACTAC
  TCATGAAGAACCGCTGGGTATGGAGCACACCTCACCTGATGGACAGTTGATTTATGCTCA
  CCTCTAACGCNTATTGAGAAGCAGCACANAAGAAGAACTGGAAAAAACTGAATTTTACTC
  CTCATCTCCGAAGATGTGATATTNTCTAAATTNNCAGCTGCCTCTTGCTTCTATAGGCAA
  GTTCCTTGGCTGNAAATTTGAACAGACTATCAGAGCTCATGGACTTCTGANATGACATGA
  CTTAAATGACTGGTNAATGGTAAACATACTGNATCATTTTCCAGCTCATTGGGTGGCCTG
  AATCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000104
• Insert Size: 2880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000104.2, NP_000095.1
• RefSeq Size: 5128 bp
• RefSeq ORF: 1632 bp
• Locus ID: 1545
• Cytogenetics: 2p22.2
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450, Tryptophan metabolism
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008]'