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Glutamine Synthetase (GLUL) (AK092874) Human Untagged Clone
CAT#: SC103453
(untagged)-Human cDNA FLJ35555 fis, clone SPLEN2004773, highly similar to GLUTAMINE SYNTHETASE (EC 6.3.1.2)
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Symbol | GLUL |
| Synonyms | GLNS|GS|PIG43|PIG59 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for AK092874 edited
GAATTCGGCACGAGGGTTTTGTAAATTTGTGTATTGGTTTTGATTTGTTTCCTCTGATAG TTTAGTATTTGGATAGTTTAGTGTTAACCTCAGCTACACTGAAGGAATAGACCTTAGTCC TCACAAGTATAAGTTCTAGCTTGGAAGCCTGGGTTCTGCAGTAGCTGTGGAACTTAAGCC TCTGAGCTCAGGGATGCAGAGGCATTGAGTTACTACCAAGGGCCTGATCTTTTCTTTAGC AGGCATCTGTGTTAATTGTTTCAAAAGGTGGTGATCAGTTTTACAGCCTATTATAAAGGA GATTTTTGCCTACTATAAAACTAATCCCCCTGAAAGAGTGAGTAAACATAACTTTTTGTG TGTTGACTTCCACAAGGGAAGGAGTTGGCACTTACACTCTGACTTTTGATTCAGTCGTCC TTCTTGAGCCATTTTTGCAGGGGATCAGTTTGGAGTGGGCGTTAACAATGTTATTCTTTT TTTCTTCTCCAGAACACCTTCCACCATGACCACCTCAGCAAGTTCCCACTTAAATAAAGG CATCAAGCAGGTGTACATGTCCCTGCCTCAGGGTGAGAAAGTCCAGGCCATGTATATCTG GATCGATGGTACTGGAGAAGGACTGCGCTGCAAGACCCGGACCCTGGACAGTGAGCCCAA GTGTGTGGAAGAGTTGCCTGAGTGGAATTTCGATGGCTCCAGTACTTTACAGTCTGAGGG TTCCAACAGTGACATGTATCTCGTGCCTGCTGCCATGTTTCGGGACCCCTTCCGTAAGGA CCCTAACAAGCTGGTGTTATGTGAAGTTTTCAAGTACAATCGAAGGCCTGCAGGTGTGTT ATAGCACAGCTATGGATACCCCTCCTCAATCTGTGAATGCTGTGAAGGGGAGGGAGAAGA CATTCTGAAATCAGCATTGGGAAGACTAGGCAATTTCAGCACTATTTTAAGAATCTGAGT GATTCTTTTCCCTGAACTTCTGCTTTGAGGAAGAGATAATATGGCCCATCTTTCTATGGT CTTCTCTGTTGGTTGCATAAAATAGCATTGGATTTGTCCAGATCTGTTTGCCGGTCTTGG AGTCCCCAGTAACAGCCTTCCTGCCTGGAATGTAGGCCAGGACAAATGTAAACCAATGGA CAAATGTTTCTCAAAAATTATAGAATGGCTCCAAGTGCCTGAGAAATGAAGAATAAATCT GACAACCAGAAGCAGCTGTCTTGTGAATAGAGGGTTAAGTGCCTGGCATTTGGTGCTTGG GAGGTGGCCAGAATGCAGATAAGGTGAAAGTTGCCCTGTTCTAAATCCACTCCCATGTGA CTTGGTTGTAACTGAGTTTAGTTAAAACTGAAGTCTTTCAGAGTCTTCCTACAGATGTAC AATTAACAGCTTCTCTCATTTTTCTGACTCGGTGATCCCAAGAAGGCCTATACTGGGTCA GTTCATACCATAGTGCACACCTCAGTTGTATAGAATCCAAGGACTATTCTCCCATCAGCA TCGGTATTCAGCATCTATGTCTTTAGATCCCTGATGGCGTATTATTGACTCTTTTTTCTA GAGACCAATTTGAGGCACACCTGTAAACGGATAATGGACATGGTGAGCAACCAGCACCCC TGGTTTGGCATGGAGCAGGAGTATACCCTCATGGGGACAGATGGGCACCCCTTTGGTTGG CCTTCCAACGGCTTCCCAGGGCCCCAGGGTAAGTCTCCTTGGGTTAGAGGTGAAATTCCC AGAAGTGTCTAACTGTGCAGGAATGCCCCTTCCCAGGGATGGGAATGACTTTCAGAATCA AGAAGCAAAATAATACAGTAAAGGCGAAACAGCCCTCACATCACCAAAGTCCAAAAATGG ATATGAATATATAAAGTAAGGTTTTAGGGGGAACGTTTGGCCCCACTGAAGCTGTGGTGA AGAGGAACTCCCCTATTGCCCCTCCCCTGCCCCGCACCTGCAGATGAAGGCAAGGATAGT GATTCAAGAGGGCAAGGCTTAAGGGCCTTCTGATCTCTGACTTTGGGATTCTCTGGATTT CTTGACTCTTAGCGTTTTGTCCTGATGCTTCTGTAGGTCCATATTACTGTGGTGTGGGAG CAGACAGAGCCTATGGCAGGGACATCGTGGAGGCCCATTACCGGGCCTGCTTGTATGCTG GAGTCAAGATTGCGGGGACTAATGCCGAGGTCATGCCTGCCCAGGTAAGTATAGCTCCAA TCCATCAATGAAGAAGGGTAGGTAGGTGCACATAGGACTTTTGCTAGTAAGGGCTGCTGA TACACCACTCACTAACCCAAAACCTAAGAACGGGTTGGAGTACAGGTGAGAAGAGAACAG GTTTAGGAGATTCTGAGTTGGAGTGAGCAGTTAGCTTTGTTTTAATGGCCAAGCTTCTCG TTTCTAGTGGGAATTTCAGATTGGACCTTGTGAAGGAATCAGCATGGGAGATCATCTCTG GGTGGCCCGTTTCATCTTGCATCGTGTGTGTGAAGACTTTGGAGTGATAGCAACCTTTGA TCCTAAGCCCATTCCTGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAGCACCAA GGCCATGCGGGAGGAGAATGGTCTGAAGTGAGTACCTTCTGCTGGGGCCATCTTTAATCT CCTGTGGCAGAAAACTTGGGAGGAGACTTAGCAATCTCTCAGCAAAGTCTCCTTTGCAGG ATGACTTGCAAATATTTGCCAAAGATGAGTAAACTTGACTTCTCAGTCTGGACGTACTTT AGGTGTTGACACTTGCCTTCACATTCTCTCATTTTGTTCCTATTTGAAAAATACCAAATA ATACTTCTGATTCACAGTGATAAATATTTGTTATAATTTATATAATATATATTAGTCATA TATCATTATATAAATATATATCGATATATATATTTGTGACATGTCATGGTGACAGGGAAA AGTTGACAAATTCATGCATTTGAAAATCTTTTAGAACTAAATTAGTAACAATACAGGCAT GTGGATAAGCTTAATGCTTATGAGGGGGAGAAAGTTTCAAATGATTAGTCTTTTCAACAA ACAGTAACTTTGTACTGCTTGTCGGGCACTGTTCTCACCACTGAGACACACAGGTAAGAA GATGCAGCCACTGCCCTCATGAAGTATTTGTTCTACTGGTATCATATTTTGGTGCACTTC ATTCTTGGCTCCATACCTGGAGACAAGGTTGGACTGCCATCTTTTCTGTTTACTCTAGGT ACATCGAGGAGGCCATTGAGAAACTAAGCAAGCGGCACCAGTACCACATCCGTGCCTATG ATCCCAAGGGAGGCCTGGACAATGCCCGACGTCTAACTGGATTCCATGAAACCTCCAACA TCAACGACTTTTCTGCTGGTGTAGCCAATCGTAGCGCCAGCATACGCATTCCCCGGACTG TTGGCCAGGAGAAGAAGGGTTACTTTGAAGATCGTCGCCCCTCTGCCAACTGCGACCCCT TTTCGGTGACAGAAGCCCTCATCCGCACGTGTCTTCTCAATGAAACCGGCGATGAGCCCT TCCAGTACAAAAATTAAGTGGACTAGACCTCCAGCTGTTGAGCCCCTCCTAGTTCTTCAT CCCACTCCAACTCTTCCCCCTCTCCCAGTTGTCCCGATTGTAACTCAAAGGGTGGAATAT CAAGGTCGTTTTTTTCATTCCATGTGCCCAGTTAATCTTGCTTTCTTTGTTTGGCTGGGA TAGAGGGGTCAAGTTATTAATTTCTTCACACCTACCCTCCTTTTTTTTCCCTATCACTGA AGCTTTTTAGTGCATTAGTGGGGAGGAGGGTGGGGAGACATAACCACTGCTTCCATTTAA TGGGGTGCACCTGTCCAATAGGCGTAGCTATCCGGACAGAGCACGTTTGCAGAAGGGGGA CTCTTCTTCCAGGTAGCTGAAAGGGGAAGACCTGACGTACTCTGGTTAGGTTAGGACTTG CCCTCGTGGTGGAAACTTTTCTTAAAAAGTTATAACCAACTTTTCTATTAAAAGTGGGAA TTAGGAGAGAAGGTAGGGGTTGGGAATCAGAGAGAATGGCTTTGGTCTCTTGCTTGTGGG ACTAGCCTGGCTTGGGACTAAATGCCCTGCTCTGAACACGAAGCTTAGTATAAACTGATG GATATCCCTACCTTGAAAGAAGAAAAGGTTCTTACTGCTTGGTCCTTGATTTATCACACA AAGCAGAATAGTATTTTTATATTTAAATGTAAAGACAAAAAACTATATGTATGGTTTTGT GGATTATGTGTGTTTTGCTAAAGGAAAAAACCATCCAGGTCACGGGGCACCAAATTTGAG ACAAATAGTCGGATTAGAAATAAAGCATCTCATTTTGAGTAGAGAGCAAGGGAAGTGGTT CTTAGATGGTGATCTGGGATTAGGCCCTCAAGACCCTTTTGGGTTTCTGCCCTGCCCACC CTCTGGAGAAGGTGGGCACTGGATTAGTTAACAGACGACACGTTACTAGCAGTCACTTGA TCTCCGTGGCTTTGGTTTAAAAGACACACTTGTCCACATAGGTTTAGAGATAAGAGTTGG CTGGTCAACTTGAGCATGTTACTGACAGAGGGGGTATTGGGGTTATTTTCTGGTAGGAAT AGCATGTCACTAAAGCAGGCCTTTTGATATTAAATTTTTTAAAAAGCAAAATTATAGAAG TTTAGATTTTAATCAAATTTGTAGGGTTTCTAGGTAATTTTTACAGAATTGCTTGTTTGC TTCAACTGTCTCCTACCTCTGCTCTTGGAGGAGATGGGGACAGGGCTGGAGTCAAAACAC TTGTAATTTTGTATCTTGATGTCTTTGTTAAGACTGCTGAAGAATTATTTTTTTTCTTTT ATAATAAGGAATAAACCCCACCTTTATTCCTTCATTTCATCTACCATTTTCTGGTTCTTG TGTTGGCTGTGGCAGGCCAGCTGTGGTTTTCTTTTGCCATGACAACTTCTAATTGCCATG TACAGTATGTTCAAAGTCAAATAACTCCTCATTGTAAACAAACTGTGTAACTGCCCAAAG CAGCACTTATAAATCAGCCTAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAACTCGAC >OriGene 5' read for AK092874 unedited
NGGGGTTCACATTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGGTTTTG TAAATTTGTGTATTGGTTTTGATTTGTTTCCTCTGATAGTTTAGTATTTGGATAGTTTAG TGTTAACCTCAGCTACACTGAAGGAATAGACCTTAGTCCTCACAAGTATAAGTTCTAGCT TGGAAGCCTGGGTTCTGCAGTAGCTGTGGAACTTAAGCCTCTGAGCTCAGGGATGCAGAG GCATTGAGTTACTACCAAGGGCCTGATCTTTTCTTTAGCAGGCATCTGTGTTAATTGTTT CAAAAGGTGGTGATCAGTTTTACAGCCTATTATAAAGGAGATTTTTGCCTACTATAAAAC TAATCCCCCTGAAAGAGTGAGTAAACATAACTTTTTGTGTGTTGACTTCCACAAGGGAAG GAGTTGGCACTTACACTCTGACTTTTGATTCAGTCGTCCTTCTTGAGCCATTTTTGCAGG GGATCAGTTTGGAGTGGGCGTTAACAATGTTATTCTTTTTTTCTTCTCCAGAACACCTTC CACCATGACCACCTCAGCAAGTCCCACTTAAATAAAGGCATCAAGCANGTGTACATGTCC CTGCCTCAGGNTGAGAAAGTCCAGGCCATGTATATCTGGATCGATGGTACTGGAGAAGGA CTGCGCTGCAAGACCCCGACCTGGACAGTGAGCCCAAGTGTGTGGAAGAAGTGCCTGAGT GGAATTTCGATGGCTCCAGTACTTTACAGTCTGAGGGTTCCAACAGTGACATGTATCTCG TGCCTGCTGCCATGTTTCGGGACCCCTTCCGTAAGGACCCTAACAAGCTGGGTGTATGTG AAGTTTTCAAGTACAATCGAAGGCCTGCNNAGTGTGTATAGCACAGCTATGGATACCCCT NCTCATCTGTATGCCTGTGTGAAGGG |
| Restriction Sites | NotI-NotI |
| ACCN | AK092874 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | AK092874.1, BAG52617.1 |
| RefSeq Size | 2265 bp |
| RefSeq ORF | 2265 bp |
| Locus ID | 2752 |
| Cytogenetics | 1q25.3 |
| Domains | gln-synt_N |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways, Nitrogen metabolism |
| Gene Summary | 'The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]' |
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