WNT6 (NM_006522) Human Untagged Clone

CAT#: SC103558

WNT6 (untagged)-Human wingless-type MMTV integration site family, member 6 (WNT6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WNT6
• Synonyms: wingless-type MMTV integration site family, member 6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006522 edited
  GAATTCGGCACCAGGAGACACAGGCGCTGGCTGCCCCGTCCGCTCTCCGCCTCCGCCGCG
  CCCTCCTCGCCCGGGATGGGCCCCCCCGCCGCCGCCGGATCCCTCGCCTCCCGGCCGCCG
  CCGTTGCGCTCGCCGCGCTCGCACTGAAGCCCGGGCCCTCGCGCGCCGCGGTTCGCCCCG
  CAGCCTCGCCCCCTGCCCACCCGGGCGGCCGTAGGGCGGTCACGATGCTGCCGCCCTTAC
  CCTCCCGCCTCGGGCTGCTGCTGCTGCTGCTCCTGTGCCCGGCGCACGTCGGCGGACTGT
  GGTGGGCTGTGGGCAGCCCCTTGGTTATGGACCCTACCAGCATCTGCAGGAAGGCACGGC
  GGCTGGCCGGGCGGCAGGCCGAGTTGTGCCAGGCTGAGCCGGAAGTGGTGGCAGAGCTAG
  CTCGGGGCGCCCGGCTCGGGGTGCGAGAGTGCCAGTTCCAGTTCCGCTTCCGCCGCTGGA
  ATTGCTCCAGCCACAGCAAGGCCTTTGGACGCATCCTGCAACAGGACATTCGGGAGACGG
  CCTTCGTGTTCGCCATCACTGCGGCCGGCGCCAGCCACGCCGTCACGCAGGCCTGTTCTA
  TGGGCGAGCTGCTGCAGTGCGGCTGCCAGGCGCCCCGCGGGCGGGCCCCTCCCCGGCCCT
  CCGGCCTGCCCGGCACCCCCGGACCCCCTGGCCCCGCGGGCTCCCCGGAAGGCAGCGCCG
  CCTGGGAGTGGGGAGGCTGCGGCGACGACGTGGACTTCGGGGACGAGAAGTCGAGGCTCT
  TTATGGACGCGCGGCACAAGCGGGGACGCGGAGACATCCGCGCGTTGGTGCAACTGCACA
  ACAACGAGGCGGGCAGGCTGGCCGTGCGGAGCCACACGCGCACCGAGTGCAAATGCCACG
  GGCTGTCGGGATCATGCGCGCTGCGCACCTGCTGGCAGAAGCTGCCTCCATTTCGCGAGG
  TGGGCGCGCGGCTGCTGGAGCGCTTCCACGGCGCCTCACGCGTCATGGGCACCAACGACG
  GCAAGGCCCTGCTGCCCGCCGTCCGCACGCTCAAGCCGCCGGGCCGAGCGGACCTCCTCT
  ACGCCGCCGATTCGCCCGACTTCTGCGCCCCCAACCGACGCACCGGCTCCCCCGGCACGC
  GCGGTCGCGCCTGCAATAGCAGCGCCCCGGACCTCAGCGGCTGCGACCTGCTGTGCTGCG
  GCCGCGGGCACCGCCAGGAGAGCGTGCAGCTCGAAGAGAACTGCCTGTGCCGCTTCCACT
  GGTGCTGCGTAGTACAGTGCCACCGCTGCCGTGTGCGCAAGGAGCTCAGCCTCTGCCTGT
  GACCCGCCGCCCGGCCGCTAGACTGACTTCGCGCAGCGGTGGCTCGCACCTGTGGGACCT
  CAGGGCACCGGCACCGGGCGCCTCTCGCCGCTCGAGCCCAGCCTCTCCCTGCCAAAGCCC
  AACTCCCAGGGCTCTGGAAATGGTGAGGCGAGGGGCTTGAGAGGAACGCCCACCCACGAA
  GGCCCAGGGCGCCAGACGGCCCCGAAAAGGCGCTCGGGGAGCGTTTAAAGGACACTGTAC
  AGGCCCTCCCTCCCCTTGGCCTCTAGGAGGAAACAGTTTTTTAGACTGGAAAAAAGCCAG
  TCTAAAGGCCTCTGGATACTGGGCTCCCCAGAACTGCTGGCCACAGGATGGTGGGTGAGG
  TTAGTATCAATAAAGATATTTAAACCACCAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006522 unedited
  GTCAGAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACCAGAGACACAG
  CGCTGGCTGCCCCGTCCGCTCTCCGCCTCCGCCGCGCCCTCCTCGCCCGGGATGGGCCCC
  CCCGCCGCCGCCGGATCCCTCGCCTCCCGGCCGCCGCCGTTGCGCTCGCCGCGCTCGCAC
  TGAAGCCCGGGCCCTCGCGCGCCGCGGTTCGCCCCGCAGCCTCGCCCCCTGCCCACCCGG
  GCGGCCGTAGGGCGGTCACGATGCTGCCGCCCTTACCCTCCCGCCTCGGGCTGCTGCTGC
  TGCTGCTCCTGTGCCCGGCGCACGTCGGCGGACTGTGGTGGGCTGTGGGCAGCCCCTTGG
  TTATGGACCCTACCAGCATCTGCAGGAAGGCACGGCGGCTGGCCGGGCGGCAGGCCGAGT
  TGTGCCAGGCTGAGCCGGAAGTGGTGGCAGAGCTAGCTCGGGGCGCCCGGCTCGGGGTGC
  GAGAGTGCCAGTTCCAGTTCCGCTTCCGCCGCTGGAATTGCTCCAGCCACAGCAAGGCCT
  TTGGACGCATCCTGCAACAGGACATTCGGGAGACGGCCTTCGTGTTCGCCATCACTGCGG
  CCGGCGCCAGCCACGCCGTCACGCAGGCCTGTTCTATGGGCGAGCTGCTGCAGTGCGGCT
  GCCAGGCGCCCCGCGGGCGGGCCCCTCCCCGGCCCTCCGGCCTGCCCGGCACCCCCGGAC
  CCCCTGGCCCCGCGGGCTCCCCGGAAGGCAGCGCCGCCTGGGGAGTGGGGGAGGCTGCNG
  CGACNACNTGGACTTCGGGGACGAGAAGTCGAGGCTCTTTATGGACGCGCGGCACAAGCG
  GGGACGCGNAGACATNCGCGCGTTGGTGGCACTGCACNAACACGAGCGGNNCAGCCTGGC
  CGTGCGAGCCCACGCGCACCGATGCAATGCCACGGCTGTCGGGATCAGCNCGCTGCGACT
  GCTGCAGAGCTGCTCATTTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006522 unedited
  NGGGTAACACTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTGG
  TGGTTTAAATATCTTTATTGATACTAACCTCACCCACCATCCTGTGGCCAGCAGTTCTGG
  GGAGCCCAGTATCCAGAGGCCTTTAGACTGGCTTTTTTCCAGTCTAAAAAACTGTTTCCT
  CCTAGAGGCCAAGGGGAGGGAGGGCCTGTACAGTGTCCTTTAAACGCTCCCCGAGCGCCT
  TTTCGGGGCCGTCTGGCGCCCTGGGCCTTCGTGGGTGGGCGTTCCTCTCAAGCCCCTCGC
  CTCACCATTTCCAGAGCCCTGGGAGTTGGGCTTTGGCAGGGAGAGGCTGGGCTCGAGCGG
  CGAGAGGCGCCCGGTGCCGGTGCCCTGAGGTCCCACAGGTGCGAGCCACCGCTGCGCGAA
  GTCAGTCTAGCGGCCGGGCGGCGGGTCACAGGCAGAGGCTGAGCTCCTTGCGCACACGGC
  AGCGGTGGCACTGTACTACGCAGCACCAGTGGAAGCGGCACAAGCAGTTCTGTTCGAGCT
  GCACGCTCTCCTGGCGGTGCCCGCGGCCGCAGCACAACAAGTCGCAGCCGCTGAAGTCCG
  GGGCGCTGCTTTTGCAAGCGCGAACGCGCCTGCCGGGGGAACCAGTGCGTCGGGTGGGGG
  GCGCAAAATTGGGCGAAATCGCGGCGAAAGGAAGTTCCCTTCGGCCGGCGGCTTAACCTT
  CCGACCGCGGGCAACAAGGGCTTTGCCTCGTTGGGGCCCTGACCCCCGAGGCGCCTGGAA
  ACGCTTCAAAAACCGGTCCCCCCTTTTGGAAAGGGAGCGATTTCTTGCACCTGTTCGCCC
  GCGCAAGTTCCCCAAGGCGGGGCTATTGCCTCCGGGCGGGTGGGGTTCCCCCCGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006522
• Insert Size: 1730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006522.3, NP_006513.1
• RefSeq Size: 1700 bp
• RefSeq ORF: 1098 bp
• Locus ID: 7475
• Cytogenetics: 2q35
• Domains: wnt
• Protein Families: Adult stem cells, Cancer stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: 'The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level. [provided by RefSeq, Jul 2008]'