JNK1 (MAPK8) (AK094000) Human Untagged Clone

CAT#: SC103740

(untagged)-Human cDNA FLJ36681 fis, clone UTERU2006547

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK8
• Synonyms: 2|PRKM8|SAPK1|SAPK1c; JNK|JNK-46|JNK1|JNK1A2|JNK21B1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK094000, the custom clone sequence may differ by one or more nucleotides
  
  
  TTTTTGTTTGTACATTTTTCTCAGAAGAGGGTTCACTACTTTTATACAATTCTCAAAAGATCCCATGACC
  CAAAGAAGATGATAAATAGTGTTGATGTGGCATCCACCATTAAGGTTAAGTGTGGTGTGCCCTGTGAGTC
  TGAATGTCTACTTAAGAACCTTAAGTAGACATTAAGAACCTTAAGAAGGTTTTTTGTTTGTTTTTGTTTT
  TTTGTTGTTGAGATGGAGCCTTGCTCCGTTGCCCAGGCTGGAGAGCAGTGGCGCAATCTCAGCTCACTGC
  AACCTCTGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTGCAGGCGCCT
  GCCCCCAAGCCCGGCTAATTTTTGTGTTTTTAGTAGAAATGGGGTTTCACCTTGTTGGTCAGGCTTGTCT
  CAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCAC
  CACACATGGCCGAAGGTTCTTCTTAAGTGGACATTCAGACTCACAAGGCACATCGCAGTTAACATCAGAA
  TCACTTCTGATGATAATATAAGTGAAGAATATAAGACAGGAGGCGCATATATTAATACCAGCAGAGCAGC
  TCCCAGTGTGTCTCTTCAGTTGGAACAGTTGTTGCAGTGGTCTACTTGCTGTCCAGAAGCCTGATAAGAG
  AAAAAGATTCCCATGGAGAAATGTTCTTCGAAGTGATAACCATGCTTACTCATAAGGAGTTGAAATGTAG
  CTTACCTGCTAGTTTTCCTCCAATAAAAATGTGTTTATCTTTCATTCTGATTTGTTGTGAAGCTTTTGCA
  CACTCTAATTTAAATCTTGGTAGCATATATCTAGTTGAGTACCCACAGTGCACCAGGCTCTATTCCAGGG
  CCCAGGAAATGGAAGTCAGTAAGACACGTGGTTCAAGCCCTCCCTGAGACAGATGGTAGTACAGAATGGT
  ATGTGGTATGATGTGCTGTAGCACAAGTTGCTGGTAGAGTGAAAAAGAAAGCTTCTACCCCAGCACTATC
  AAACCAACTTCAGAAACAGTGAATTACAGGAAAGATTAGTACTTCCTTTTAATATGATCCATTGTTGAGT
  GTCAAGGAATTGTTATTAATTAACATCCTTGAATCTTAGGCCGACATTTAACTGACTGTCATTGTAAGGA
  CACTGTTTGAAGTACTTCACATGTATAAAAATTTCCACTTAAACCATACATGCGTTGTGAGATTGGCTCT
  TAGACTTTGAAAAGTTCATTTTTGTTTACTTCTTTTACAGAATTGATATATAAGGAAGTTATGGACTTGG
  AGGAGAGAACCAAGAATGGAGTTATACGGGGGCAGCCCTCTCCTTTAGGTGCAGCAGTGATCAATGGCTC
  TCAGCATCCATCATCATCGTCGTCTGTCAATGATGTGTCTTCAATGTCAACAGATCCGACTTTGGCCTCT
  GATACAGACAGCAGTCTAGAAGCAGCAGCTGGGCCTCTGGGCTGCTGTAGATGACTACTTGGGCCATTGG
  GGGGTGGGAGGGATGGGGAGTCGGTTAGTCATTGATAGAACTACTTTGAAAACAATTCAGTGGTCTTATT
  TTTGGGTGATTTTTCAAAAAATGTAGAATTCATTTTGTAGTAAAGTAGTTTATTTTTTTTAATTTCAAGT
  GATGTAATTTAAAACCTAAGTTGTGTTTCAAAACAGCAACAAAACTGTATTGTATTTTTTTTGCTGTAAT
  TAACTGTATAATGTAAACCTAATTATTTTATCATGGTTTAAATTTTTTGCATATTTGCTTTATCTTATGC
  TGCTGATTTTTTTAACTGAATTTGTAAGATTTTGTTTATCAAAGCAACTATTATGTGGTAACTTGCCTAT
  ATCATGAATTATTTAAGATTTTTATAGTTTTTTTTAATTAGAATTTATTTCAGATGTTTTGTTCATGATA
  CTATCCTTCAGGGTTATGTGCTTATCAATGAAATAACCCCAGAGGAGTGAGGGAAAATAACTTGTAGCCA
  GTTATATTCAGGAATAACTACTGTAAATGATGAACGTGTTAGGAGACCTCCAATATTTGCTACTTGCCAA
  TCCTAATTTAGTTACAAGAATTGGTAGGCAATCCTACTTAATTTTGGCAAAAGCCCCGTCATCTAAATGG
  CAGAATAACTCAGAGCATGTCTTTGAAGATGCTGGGCGTCTACCACCACCTTATGTCCCCACCCTACCCA
  ACAAAAATAAGTAAAAAGAATATGGTGTATTCTACAAATTTGTGGCATGCTCAAAGTTTATGATCACATA
  AAGGCAAGAGGATACTTCATGAATAATACATTTCAATGCAAATAAACAGATGGTTCACTTCT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK094000 unedited
  GGTGCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGTTTTTAGT
  AGAAAGGGGTTTCACCTTGTTGGTCAGGCTTGTCTCAAACTCCTGACCTCAGGTGATCCA
  CCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACACATGGCCGAAG
  GTTCTTCTTAAGTAGACATTCAGACTCACAAGGCACATCGCAGTTAACATCAGAATCACT
  TCTGATGATAATATAAGTGAAGAATATAAGACAGGAGGCGCATATATTAATACCAGCAGA
  GCAGCTCCCAGTGTGTCTCTTCAGTTGGAACAGTTGTTGCAGTGGTCTACTTGCTGTCCA
  GAAGCCTGATAAGAGAAAAAGATTCCCATGGAGAAATGTTCTTCGAAGTGATAACCATGC
  TTACTCATAAGGAGTTGAAATGTAGCTTACCTGCTAGTTTTCCTCCAATAAAAATGTGTT
  TATCTTTCATTCTGATTTGTTGTGAAGCTTTTGCACACTCTAATTTAAATCTTGGTAGCA
  TATATCTAGTTGAGTACCCACAGTGCACCAGGCTCTATTCCAGGGCCCAGGAAATGGAAG
  TCAGTAAGACACGTGGTTCAAGCCCTCCCTGAGACAGATGGTAGTACAGAATGGTATGTG
  GTATGATGTGCTGTAGCACAAGTTGCTGGTAGAGTGAAAAAGAAAGCTTCTACCCCAGCA
  CTATCAAACCAACTTCAGAAACAGTGAATTACAGGAAAGATTAGTACTTNCTTTTATATG
  ATCCATTGTTGAGTGTCAAGGAATTGNTATTAANTAACATCCTTGAATCTTTAGCCGACA
  TTTAACTGACTGTCATTGTAAGGACACTGTTTGAAGTACTTCACATGTATAAAAATTTCC
  ACTTAAACATACATGCGTTGTGAGAATGCCTCTTAGAA
  
• Restriction Sites: NotI-NotI
• ACCN: AK094000
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK094000.1
• RefSeq Size: 2372 bp
• RefSeq ORF: 2372 bp
• Locus ID: 5599
• Cytogenetics: 10q11.22
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]'