UBL4A (AK000405) Human Untagged Clone

CAT#: SC104199

(untagged)-Human cDNA FLJ20398 fis, clone KAT00580, highly similar to M35604 Human glucose-6-phosphate dehydrogenase

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBL4A
• Synonyms: DX254E|DXS254E|G6PD|GDX|GET5|MDY2|TMA24|UBL4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK000405, the custom clone sequence may differ by one or more nucleotides
  
  
  AAGGACGCCGTCGCGACCGCCATGCAGCTGACGGTGAAGGCGCTGCAGGGCCGCGAGTGCAGCCTGCAGG
  TGCCAGAGGACGAGCTGGTGTCCACGCTGAAGCAGCTGGTCTCCGAGAAGCTGAACGTCCCAGTGCGCCA
  GCAGCGGCTGCTGTTCAAGGGCAAGGCCCTGGCAGATGGGAAACGACTCTCGGATTATAGCATCGGGCCC
  AACTCCAAGCTCAACCTAGTGGTCAAACCCCTGGAGAAGGTGCTACTAGAAGAAGGCGAGGCCCAGAGGC
  TGGCCGACTCCCCACCCCCGCAGGTCTGGCAGCTGATCTCCAAAGTCTTGGCCCGCCACTTCAGTGCGGC
  AGATGCCAGCAGGGTCCTGGAACAGCTACAGAGGGATTACGAGAGGTCCCTGAGTCGCCTGACGCTGGAC
  GACATCGAACGGTTGGCCAGCCGCTTCCTGCACCCTGAAGTGACTGAGACAATGGAGAAGGGCTTCTCCA
  AATAGAATTCTCGGAGCATGGGGAGGTGCCCAACGCCAGGCTACCGCTGCATGTCGCACTAAGTGTGTTC
  TCCTGTTGCAGTTGGGCTCATCATCGTCATAGCTGGCATGTACCTGGCTCTGGCCAGGTGCTAGGCACTC
  CTCACAGCTTGACCTGGGTTTGCTTCCACACCCTCAGAAGAGGAGAGCCGGCACAGCAGAGGCACCGGCG
  ATGAAGTGGCACAGCCCCAGTCGGAATCCAGCGGCTTCTGAAAGTGCCTTGGTGTGAGAAGGAGGAAGGG
  GCCGCTTGGAGAGCTGGGCTCTCGCATGTCATGTTTAGCCCACTGAGAATATACCCTCAGGTGACTCCCT
  CCCGATCCTGAAAGGGAAAGCAGCTGTCACCTGACTTCTGGCGGGTCCAACATAGCCCTCAGCTTACCTC
  TGCAGGAGGCCGAGTGACAGCCAGCCCTGGAACCCCCCGACCCCCGCAGCTGCTGCAGCCAGTGTGCCAG
  TGTGCGTTCGACAAATGGAAAAGCAGATTGGGGCCAGGGAGTCAGCAAGGCACCCCAGCTCTGTGATGTG
  ACTTTGGGCGAGTCTCAGTGCCACTTGGTCCCCAGCCATGGACTCTATACAGTGAGGGCCACTCACCGAC
  CTGAGTCAGTGTCCTCTGTCTCACAGGGCCCCTCTCCCTTCTGTTCAGTAAACAAACTGAAGCCAAAAAT
  GAAGCCGTGGCCAAGCGTGACCCAGAGATGGGGTGTCCTGGCCCTTAGTGACACAGCTCCTCTCTGGGGC
  ACCCTATCTGCTTGTCTCGTCCAGGAAGCGTTAGGGCTGATGGGTGACTCAGCAAGGCAGTGTCAGAGCT
  GGGACTGGGCGTAGGCCTTTGTCCTCATCCCCAGCACTGGCCTCCCTGTGGAAGATGAACATATCCCAGC
  CACCTGTGTACAGGGGCTCACTTTGTGTGCTCCTTGTTGCCTGAGAAGAAACCTTGGGGTGCCAGGGTGG
  GGGCAGAAGCATGGGCTGGGTTCCGGTTCATCCTCCTCCACCCTGCCGTGTGTGTGGGCACAAGAGGACA
  TCTAACCACCTGCTCCTTGGAGGAGGCCCCCAGGGGTGGTAGAGGCTGGAAGGAAGCCACATCAGGAGGA
  CGCCACTCCGGCCCTTCACCCTTGCCAAGTGAGCTGCTCACAGTGTGGTCAGGGCTGCGCGTGCTGGAGG
  CCCTCCTGCCTGGGCCTTGTGGGGCAAATATTGGGTCCCCAGGCTGGAAAGATGGACAGAGGCCCAATGG
  GTGAAGGCTTTGAAGAGCACACAGAAGCCCCTGGCCCCCCACGAGAGCTGGAGAGCCATGTATATGGCTT
  CAAAGCCACCTACGGCAGGGACACACTCGTGAGCATGTGTGGCCTGCAGTTCAGGTGATACATTTACCAG
  TGTTCTTGTTTGTGTGGTGCCAGGAAATTGATTTTGGAAAAAGTGAAATAACATTAAAGGTGAATGTGAG
  GCTTCTACTTTTATCCAAAAGGAGCTATATTAGCTAGGCTGTTTCTGATATCCAATCATTGGTTTAACAA
  TAAAGGCAATTTGTTTAATCAGTTAACGGAAATTTCTTGGCTTATGAAAGTGAAAAGTCCAGTGGTATTG
  GCATTGGCAGCAGGTGAGCAATTTCACCCAGTGTCTTCTGCCTCCCTCTGCGTTGGTATCTGCTACATCC
  CAGGCCACCACCTCCGAGGATGAAAAGATGGCTGCCTGCAGCTTCCACGGAATCCCTCCCTCACTGCCAG
  AGCAGCATCTTCTCTGTGTACCACCTCTGCTGTCTCAGATGCCCCCAGCAAATAAACACTCTTCTCGTTG
  GTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK000405 unedited
  NAAAGGGGAANNAAANAANNAATCACNCNCGCGCCTGTCCAGCATTTGTNATACGACTCA
  CTATAGGGCGGCCGCNGATTCGGCACCAGGTCGCGACCGCCATGCAGCTGACGGTGAAGG
  CGCTGCAGGGCCGCGAGTGCAGCCTGCAGGTGCCAGAGGACGAGCTGGTGTCCACGCTGA
  AGCAGCTGGTCTCCGAGAAGCTGAACGTCCCAGTGCGCCAGCAGCGGCTGCTGTTCAAGG
  GCAAGGCCCTGGCAGATGGGAAACGACTCTCGGATTATAGCATCGGGCCCAACTCCAAGC
  TCAACCTAGTGGTCAAACCCCTGGAGAAGGTGCTACTAGAAGAAGGCGAGGCCCAGAGGC
  TGGCCGACTCCCCACCCCCGCAGGTCTGGCAGCTGATCTCCAAAGTCTTGGCCCGCCACT
  TCAGTGCGGCAGATGCCAGCAGGGTCCTGGAACAGCTACAGAGGGATTACGAGAGGTCCC
  TGAGTCGCCTGACGCTGGACGACATCGAACGGTTGGCCAGCCGCTTCCTGCACCCTGAAG
  TGACTGAGACAATGGAGAAGGGCTTCTCCAAATAGAATTCTCGGAGCATGGGGAGGTGCC
  CAACGCCAGGCTACCGCTGCATGTCGCACTAAGTGTGTTCTCCTGTTGCAGTTGGGCTCA
  TCATCGTCATAGCTGGCATGTACCTGGCTCTGGCCAGGTGCTAGGCACTTCTCACAGCTT
  GACCTGGGTTTGCTTCCACACCCTCAGCAGAGGAGAGCCCGCACAGCAGAAGCACCGGCG
  ATGAAGTTGCACAGCCCCAGTCGGAATCCAGCGCCTTCTGAAGTGCCTGTTGTTGGAAAC
  GAAGGAAGGGCCCGCTGAAAACCTGGGCTCTCGCATGTCATGTTTACCCCCTTGAGAATA
  CCCTCAGGGACTCCCTCCCGATCTGAAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK000405 unedited
  AGTTAAAATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACCAACCAAAAAAATGTTT
  ATTTGCTGGGGGCATCTGAAACAGCAGAGGTGGTACACAAAAAAAATGCTGCTCTGGCAG
  TGAGGGAGGGATTCCGTGGAAACTGCAGGCAGCCATCTTTTCATCCTCGGAGGTGGTGGC
  CTGGGATGTAGCAAATACCAACGCAAAGGGAGGCAGAAAACACTGGGTGAAATTGCTCAC
  CTGCTGCCAATGCCAATACCACTGGACTTTTCACTTTCATAAGCCAAGAAATTTCCGTTA
  ACTGATTAAACAAATTGCCTTTATTGTTAAACCAATGATTGGATATCAAAAACAGCCTAA
  CTAATATAGCTCCTTTTGGATAAAAGTAGAAGCCTCACATTCACCTTTAATGTTATTTCA
  CTTTTTCCAAAATCAATTTCCTGGCACCACACAAACAAGAACACTGGTAAATGTATCACC
  TGAACTGCAGGCCACACATGCTCACGAGTGTGTCCCTGCCGTAAGTGGCTTTGAAACCAT
  ATACATGGCTCTCCAACTCTCGTGGGGGGCCAGGGGCTTCTGTGTGCTCTTCAAAGCCTT
  CACCCATTGGGCCTCTGTCCATCTTTCCAGCCTGGGGACCCAATATTTGCCCCACAAAGC
  CCATGCAAGAGGGCCTTCAGCACGCGCAGCCCTTGACACACTGTGAGCAGCTCACTTGCA
  AAGGTGAAGGGCCCGATTGGCGTCCTCCCTGAGTGGCTTNCTTCCAGCCTCTACACCCCT
  GGGGGCCTCCTCCAAAAACAGGGGGTTAAATGTCCCCTTGGGCCCACCACCCGCAGGGTG
  GGA
  
• Restriction Sites: NotI-NotI
• ACCN: AK000405
• ORF Size: 2342 bp
• Insert Size: 2500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: AK000405.1
• RefSeq Size: 2342
• RefSeq ORF: 2342
• Locus ID: 8266
• Domains: UBQ
• Protein Families: Druggable Genome
• Gene Summary: As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303). [UniProtKB/Swiss-Prot Function]