IRF5 (NM_032643) Human Untagged Clone

CAT#: SC104269

IRF5 (untagged)-Human interferon regulatory factor 5 (IRF5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRF5
• Synonyms: SLEB10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC104269 sequence for NM_032643 edited (data generated by NextGen Sequencing)
  ATGAACCAGTCCATCCCAGTGGCTCCCACCCCACCCCGCCGCGTGCGGCTGAAGCCCTGG
  CTGGTGGCCCAGGTGAACAGCTGCCAGTACCCAGGGCTTCAATGGGTCAACGGGGAAAAG
  AAATTATTCTGCATCCCCTGGAGGCATGCCACAAGGCATGGTCCCAGCCAGGACGGAGAT
  AACACCATCTTCAAGGCCTGGGCCAAGGAGACAGGGAAATACACCGAAGGCGTGGATGAA
  GCCGATCCGGCCAAGTGGAAGGCCAACCTGCGCTGTGCCCTTAACAAGAGCCGGGACTTC
  CGCCTCATCTACGACGGGCCCCGGGACATGCCACCTCAGCCCTACAAGATCTACGAGGTC
  TGCTCCAATGGCCCTGCTCCCACAGACTCCCAGCCCCCTGAGGATTACTCTTTTGGTGCA
  GGAGAGGAGGAGGAAGAAGAGGAAGAGCTGCAGAGGATGTTGCCAAGCCTGAGCCTCACA
  GAGGATGTCAAGTGGCCGCCTACTCTGCAGCCGCCCACTCTGCRGCCGCCTACTCTGCAG
  CCGCCCACTCTGCAGCCGCCCGTGGTGCTGGGTCCCCCTGCTCCAGACCCCAGCCCCCTG
  GCTCCTCCCCCTGGCAACCCTGCTGGCTTCAGGGAGCTTCTCTCTGAGGTCCTGGAGCCT
  GGGCCCCTGCCTGCCAGCCTGCCCCCTGCAGGCGAACAGCTCCTGCCAGACCTGCTGATC
  AGCCCCCACATGCTGCCTCTGACCGACCTGGAGATCAAGTTTCAGTACCGGGGGCGGCCA
  CCCCGGGCCCTCACCATCAGCAACCCCCATGGCTGCCGGCTCTTCTACAGCCAGCTGGAG
  GCCACCCAGGAGCAGGTGGAACTCTTCGGCCCCATAAGCCTGGAGCAAGTGCGCTTCCCC
  AGCCCTGAGGACATCCCCAGTGACAAGCAGCGCTTCTACACGAACCAGCTGCTGGATGTC
  CTGGACCGCGGGCTCATCCTCCAGCTACAGGGCCAGGACCTTTATGCCATCCGCCTGTGT
  CAGTGCAAGGTGTTCTGGAGCGGGCCTTGTGCCTCAGCCCATGACTCATGCCCCAACCCC
  ATCCAGCGGGAGGTCAAGACCAAGCTTTTCAGCCTGGAGCATTTTCTCAATGAGCTCATC
  CTGTTCCAAAAGGGCCAGACCAACACCCCACCACCCTTCGAGATCTTCTTCTGCTTTGGG
  GAAGAATGGCCTGACCGCAAACCCCGAGAGAAGAAGCTCATTACTGTACAGGTGGTGCCT
  GTAGCAGCTCGACTGCTGCTGGAGATGTTCTCAGGGGAGCTATCTTGGTCAGCTGATAGT
  ATCCGGCTACAGATCTCAAACCCAGACCTCAAAGACCGCATGGTGGAGCAATTCAAGGAG
  CTCCATCACATCTGGCAGTCCCAGCAGCGGTTGCAGCCTGTGGCCCAGGCCCCTCCTGGA
  GCAGGCCTTGGTGTTGGCCAGGGGCCCTGGCCTATGCACCCAGCTGGCATGCAATAA
  
  Clone variation with respect to NM_032643.3
  501 c=>t;524 g=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032643 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGCTCCCTG
  GCGCAGCCACGCAGGCGCACCGCAGACAGACCCCTCTGCCATGAACCAGTCCATCCCAGT
  GGCTCCCACCCCACCCCGCCGCGTGCGGCTGAAGCCCTGGCTGGTGGCCCAGGTGAACAG
  CTGCCAGTACCCAGGGCTTCAATGGGTCAACGGGGAAAAGAAATTATTCTGCATCCCCTG
  GAGGCATGCCACAAGGCATGGTCCCAGCCAGGACGGAGATAACACCATCTTCAAGGCCTG
  GGCCAAGGAGACAGGGAAATACACCGAAGGCGTGGATGAAGCCGATCCGGCCAAGTGGAA
  GGCCAACCTGCGCTGTGCCCTTAACAAGAGCCGGGACTTCCGCCTCATCTACGACGGGCC
  CCGGGACATGCCACCTCAGCCCTACAAGATCTACGAGGTCTGCTCCAATGGCCCTGCTCC
  CACAGACTCCCAGCCCCCTGAGGATTACTCTTTTGGTGCAGGAGAGGAGGAGGAAGAAGA
  GGAAGAGCTGCAGAGGATGTTGCCAAGCCTGAGCCTCACAGAGGATGTCAAGTGGCCGCC
  CACTCTGCAGCCGCCCACTCTGCGGCCGCCTACTCTGCAGCCGCCCACTCTGCAGCCGCC
  CCGTGGTGCTGGGGTCCCCCNTGCTCCAGACCCCAGCCCCCTGGCTCCTCCCCCTGGCAA
  CCCTGCTGGCTTCAGGGAGCTTCTCTTCTGAGTCCTGGAGCCTGNGCCCCTGCCTGCCAG
  CCTGCCCCCTGCANGCGAACAGCTCCTGCCAGACCTGCTGATCAGCCCCCCATGCTGNCT
  CTGACGANCTGGAGNACAAGNTTCAGTACCGGGGGCGGNCACCCCGGGCCTCACATCAGC
  ACCCCCATGGTGNCGCTCTCTCANCAGCTGGAGCACCAGAACAGGTGGACTCTTCGCCCA
  TAGCTGGAGCAGGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032643 unedited
  CCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTATACAGGAAAAGGTTTAT
  TTGGCTCACAGTTCTGATGGCTGGGCATCAAGATTGGGCATCTGCACCTGGTAAGGGCCT
  GGGGCAGCTTCCACTCAATGGCTGAAGGCAGAAAGTGGGCTGGAGTGTGCAGAGATGACA
  CAGATGAAGTGAGAGCGGGTAGGTGCCAGGCTCTTTAACAACCAGCTCTGGGGGGACTAA
  GAGAACTCACTCACTGTCCCCACCTCGGGGGACAATATAATAAATATATTAAATATATCA
  GGATGATTTACATGCACTGCCCTGATATATTTAATAGCACAGGAATATACACAAGAGAGT
  GACAGATTTGAATGGTCCCAAATGCAGCTGCAATGCTAGCCCACCCTTTTATGGGAAAAC
  TGATTCTGACAGTTTTCCAGACTGTGCACACATTTAAAATTCCACACCCTTGCTTCAGCC
  AGCTTCCTCCTGGAAGGCAAAGCCAGCCAGGTGAGTGTTTATGAAGGCCCAACTGACCCA
  TCAGCCCAGAAGCCAGGCCCTCCCAGTGCCAATGGTCGCAAGCTGAGCGCAAGTGCTTGG
  GGAGCGGGCCAGTTGGCATCCGAAACAGGGCCTGGTGGAGCAGCATGCGAGCAACTGGTA
  GTAGTATAAGGGAAAGAACAAGCTGAGCCTGTTCTCTACATTCATTATTCGCAAAAATAC
  ATAANAAATCTATTTCTGCCTCCAGGCTCTTGGACTCTCAGGAAAGGCGGTAGCTGCCAG
  CCCTCGAGCATCTGATTTGATACCCTAGCGCCTCACTCATTTTATTCTCAGAAGACACGG
  CAGATGAGGCAGCGGAGTCTCACACCAAGGGAATTACGCCCATGCCCTGCATAGCGCTCG
  GGGGGGGACTTGGTCACACCACTCTGTCTTTTGTTGCACAAGGAATGGGCATTCCAAAAG
  TCGCCACCTTAGCATTCCTCTATCACTAGCCCCAGGTGGAGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032643
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032643.3, NP_116032.1
• RefSeq Size: 2771 bp
• RefSeq ORF: 1497 bp
• Locus ID: 3663
• Cytogenetics: 7q32.1
• Domains: IRF
• Protein Families: Transcription Factors
• Protein Pathways: Toll-like receptor signaling pathway
• Gene Summary: 'This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site, compared to variant 9. The encoded isoform (b) is shorter, compared to isoform d. Variants 2, 3, and 6 encode the same isoform.