myosin heavy chain 9 (MYH9) (AK025393) Human Untagged Clone

CAT#: SC104993

(untagged)-Human cDNA: FLJ21740 fis, clone COLF4800, highly similar to HUMMYONM Human nonmuscle myosin heavy chain (NMHC) mRNA


Reconstitution Protocol

USD 660.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MYH9
Synonyms BDPLT6|DFNA17|EPSTS|FTNS|MHA|NMHC-II-A|NMMHC-IIA|NMMHCA
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for AK025393, the custom clone sequence may differ by one or more nucleotides


AGTCAGTATTGTATATGAATTTTCTGCCATGTGGTAGAAAGAGGGTGCCTGTCTTTGTACTCTGTGTGAA
CAGGCACACCTGTTTACCTGTCTTGGTTTCACCGGTTTTATAGGAGTTGCAACAGAGGATGGCATTTTAC
ACTTACTTTTGTGCCTTTCCTGTTGTAATTGGTCCTGTTTGCCTGAGCTGTTTTTGTGCTACTGTTGTGT
GTGAAGATGTCCGACACCTAAACATTTTGCGGGAAGCTTTGCCTGCTTTCCTGAGAGTGGTCCTGTCTAG
CTCAGCTGTGGCTCCCAAGACTCCACTTTGCTTCTTTCTGGTGGGAGCAGCCAGGCACCTGGTTTCCCTC
TCGAATTCACTTTGAGATGTGTGGGCTGTGCTGTGGCCGCCCCTGGCCTGGCCGGGCCCGCTGACCCTCT
GCTTCTACCTCTGCCCCCAAGCGCGGGGACCTGCCGTTTGTCGTGCCCCGCCGAATGGCCCGGAAAGGCG
CCGGGGATGGCTCCGACGAAGAGGTAGATGGCAAAGCGGATGGGGCTGAGGCCAAACCTGCCGAATAAGC
CTCTTCTCCTGCAGCCTGAGATGGATGGACAGACAGACACCACAGCCTCCCCTTCCCAGACCCCGCAGCA
CGCCTCTCCCCACCTTCTTGGGACTGCTATGAACATGCCTCCTCCTGCCCTCCGCCCCGTCCCCCCATCC
CGTTTCCCTCCAGGTGTTGTTGAGGGCATTTGGCTTCCTCTGCTGCATCCCCTTCCAGCTCCCTCCCCTG
CTCAGAATCTGATACCAAAGAGACAGGGCCCGGGCCCAGGCAGAGAGCGACCAGCAGGCTCCTCAGCCCT
CTCTTGCCAAAAAGCACAAGATGTTGAGGCGAGCAGGGCAGGCCCCCGGGGAGGGGCCAGAGTTTTCTAT
GAATCTATTTTTCTTCAGACTGAGGCCTTTTGGTAGTCGGAGCCCCCGCAGTCGTCAGCCTCCCTGACGT
CTGCCACCAGCGCCCCCACTCCTCCTCCTTTCTTTGCTGTTTGCAATCACACGTGGTGACCTCACACACC
TCTGCCCCTTGGGCCTCCCACTCCCATGGCTCTGGGCGGTCCAGAAGGAGCAGGCCCTGGGCCTCCACCT
CTGTGCAGGGCACAGAAGGCTGGGGTGGGGGGAGGAGTGGATTCCTCCCCACCCTGTCCCAGGCAGCGCC
ACTGTCCGCTGTCTCCCTCCTGATTCTAAAATGTCTCAAGTGCAATGCCCCCTCCCCTCCTTTACCGAGG
ACAGCCTGCCTCTGCCACAGCAAGGCTGTCGGGGTCAAGCTGGAAAGGCCAGCAGCCTTCCAGTGGCTTC
TCCCAACACTCTTGGGGACCAAATATATTTAATGGTTAAGGGACTTGTCCCAAGTCTGACAGCCAGAGCG
TTAGAGGGGCCAGCGGCCCTCCCAGGCGATCTTGTGTCTACTCTAGGACTGGGCCCGAGGGTGGTTTACC
TGCACCGTTGACTCAGTATAGTTTAAAAATCTGCCACCTGCACAGGTATTTTTGAAAGCAAAATAAGGTT
TTCTTTTTTCCCCTTTCTTGTAATAAATGATAAAATTCCGAGTCTTTCTCACTGCCTTTGTTTAGAAGAG
AGTAGCTCGTCCTCACTGGTCTACACTGGTTGCCGAATTTACTTGTATTCCTAACTGTTTTGTATATGCT
GCATTGAGACTTACGGCAAGAAGGCATTTTTTTTTTTTTAAAGGAAACAAACTCTCAAATCATGAAGTGA
TATAAAAGCTGCATATGCCTACAAAGCTCTGAATTCAGGTCCCAGTTGCTGTCACAAAGGAGTGAGTGAA
ACTCCCACCCTACCCCCTTTTTTATATAATAAAAGTGCCTTAGCATGTGTTGCAGCTGTCACCACTACAG
TAATCTGGTTTACAGATGTTTTCCACTGAGCATCACAATAAAGAGAACCATGTGCTAAAAAAAAAAAAAA
AAAAAAAA


>OriGene 5' read for AK025393 unedited
CCCAGCTCGTTTTTGTATACGACTCATATAGGCGGCCTGCGAATCGGCACCAGGTCGGCC
GANAAAACTGAAGGAGTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTAC
AAGGACCAGGCCGACAAGGCATCTACCCGCCTGAAGCAGCTCAAGCGGCAGCTGGAGGAG
GCTCGAAGAGGAGGCCCAGCGGGCCAACGCCTCCCGCCGGAAACTGCAGCGCGAGCTGGA
GGACGCCACTGAGACGGCCGATGCCATGAACCGCGAAGTCAGCTCCCTAAAGAACAAGCT
CAGGCGCGGGGACCTGCCGTTTGTCGTGCCCCGCCGAATGGCCCGGAAAGGCGCCGGGGA
TGGCTCCGACGAAGAGGTAGATGGCAAAGCGGATGGGGCTGAGGCCAAACCTGCCGAATA
AGCCTCTTCTCCTGCAGCCTGAGATGGATGGACAGACAGACACCACAGCCTCCCCTTCCC
AGACCCCGCAGCACGCCTCTCCCCACCTTCTTGGGACTGCTGTGAACATGCCTCCTCCTG
CCCTCCGCCCCGTCCCCCCATCCCGTTTCCCTCCAGGTGTTGTTGAGGGCATTTGGCTTC
CTCTGCTGCATCCCCTTCCAGCTCCCTCCCCTGCTCAGAATCTGATACCAAAGAGACATG
GCCCGGGCCCAGGCAGAGAGCCACCAGCAGGCTCCTCAGCCCTCTCTTGCCAAAAGCACA
AGATGTTGAGGCGAGCAGGGCAGGCCCCCGGNGAGGGGCTCAGAGNTTTCTATGAATCTA
TGTTTCTTCAGACTGAGGCCTNTTTGGTAGTCGNAGCCCCCGCAGTCGTCAGCCTCCCTG
ACGTATGGCAGCAGNGCCCNCACTCTCCTNCTTTTTTTGCTGATCCAACACCACGTGGGG
ACTNACACACCTCTGCCCATGGGCCTNCCACTCT
>OriGene 3' read for AK025393 unedited
GGTCCTCTATGNACGCGGCCGCCTTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTAGCACATGGGTTCTCTTTATTGGGATGCTCAGTGGAAAACATCTGTAAACCAG
CTTACTGTAGTGGTGACAGCTACAACACATGCTAAGGCACTTTTATTATATAAAAAAGGG
GGTAGGGTGGGAGTTTCACTCACTCCTTTGTGACAGCAACTGGGACCTGAATTCAAAGCT
TTGTAGGCATATGCAGCTTTTATATCACTTCATGATTTGAGAGTTTGTTTCCTTTAAAAA
AAAAAAATGCCTTCTTGCCGTAAGTCTCAATGCAGCATATACAAAACAGTTAGGAATACA
AGTAAATTCGGCAACCAGTGTAGACCAGTGAGGACGAGCTACTCTCTTCTAAACAAAGGC
AGTGAGAAAGACTCGGAATTTTATCATTTATTACAAGAAAGGGGAAAAAAGAAAACCTTA
TTTTGCTTTCAAAAATACCTGTGCAGGTGGCAGATTTTTAAACTATACTGAGTCAACGGT
GCAGGTAAACCACCCTCGGGCCCAGTCCTAGAGTAGACACAAGATCGCCTGGGAGGGCCG
CTGGCCCCTCTAACGCTCTGGCTGTCAGACTTGGGACAAGTCCCTTAACCATTAAATATA
TTTTGTCCCCAAGAGTGTTGGGAGAAGCCACTGGGAGGCTGCTGGCCTTTCCAGCTTGAC
CCCAACANCCTTGCTGTGGCAAAAGCAGGCTGTCCCGGGTAAAAGGAAGGGAGGGGGCAT
GCACTTTGAAACTTTTTAGAATCCAGAGGGAGACCCCGGACAGTGGCGCTGCCTGGGACA
GGGGGGGGAAGGAATCCATCCTCCCCCACCCCGACTTTTT
Restriction Sites NotI-NotI     
ACCN AK025393
Insert Size 1850 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq AK025393.1
RefSeq Size 1968 bp
RefSeq ORF 1968 bp
Locus ID 4627
Cytogenetics 22q12.3
Protein Families Druggable Genome
Protein Pathways Regulation of actin cytoskeleton, Tight junction, Viral myocarditis
Gene Summary 'This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]'

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