myosin heavy chain 9 (MYH9) (AK025393) Human Untagged Clone

CAT#: SC104993

(untagged)-Human cDNA: FLJ21740 fis, clone COLF4800, highly similar to HUMMYONM Human nonmuscle myosin heavy chain (NMHC) mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYH9
• Synonyms: BDPLT6|DFNA17|EPSTS|FTNS|MHA|NMHC-II-A|NMMHC-IIA|NMMHCA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK025393, the custom clone sequence may differ by one or more nucleotides
  
  
  AGTCAGTATTGTATATGAATTTTCTGCCATGTGGTAGAAAGAGGGTGCCTGTCTTTGTACTCTGTGTGAA
  CAGGCACACCTGTTTACCTGTCTTGGTTTCACCGGTTTTATAGGAGTTGCAACAGAGGATGGCATTTTAC
  ACTTACTTTTGTGCCTTTCCTGTTGTAATTGGTCCTGTTTGCCTGAGCTGTTTTTGTGCTACTGTTGTGT
  GTGAAGATGTCCGACACCTAAACATTTTGCGGGAAGCTTTGCCTGCTTTCCTGAGAGTGGTCCTGTCTAG
  CTCAGCTGTGGCTCCCAAGACTCCACTTTGCTTCTTTCTGGTGGGAGCAGCCAGGCACCTGGTTTCCCTC
  TCGAATTCACTTTGAGATGTGTGGGCTGTGCTGTGGCCGCCCCTGGCCTGGCCGGGCCCGCTGACCCTCT
  GCTTCTACCTCTGCCCCCAAGCGCGGGGACCTGCCGTTTGTCGTGCCCCGCCGAATGGCCCGGAAAGGCG
  CCGGGGATGGCTCCGACGAAGAGGTAGATGGCAAAGCGGATGGGGCTGAGGCCAAACCTGCCGAATAAGC
  CTCTTCTCCTGCAGCCTGAGATGGATGGACAGACAGACACCACAGCCTCCCCTTCCCAGACCCCGCAGCA
  CGCCTCTCCCCACCTTCTTGGGACTGCTATGAACATGCCTCCTCCTGCCCTCCGCCCCGTCCCCCCATCC
  CGTTTCCCTCCAGGTGTTGTTGAGGGCATTTGGCTTCCTCTGCTGCATCCCCTTCCAGCTCCCTCCCCTG
  CTCAGAATCTGATACCAAAGAGACAGGGCCCGGGCCCAGGCAGAGAGCGACCAGCAGGCTCCTCAGCCCT
  CTCTTGCCAAAAAGCACAAGATGTTGAGGCGAGCAGGGCAGGCCCCCGGGGAGGGGCCAGAGTTTTCTAT
  GAATCTATTTTTCTTCAGACTGAGGCCTTTTGGTAGTCGGAGCCCCCGCAGTCGTCAGCCTCCCTGACGT
  CTGCCACCAGCGCCCCCACTCCTCCTCCTTTCTTTGCTGTTTGCAATCACACGTGGTGACCTCACACACC
  TCTGCCCCTTGGGCCTCCCACTCCCATGGCTCTGGGCGGTCCAGAAGGAGCAGGCCCTGGGCCTCCACCT
  CTGTGCAGGGCACAGAAGGCTGGGGTGGGGGGAGGAGTGGATTCCTCCCCACCCTGTCCCAGGCAGCGCC
  ACTGTCCGCTGTCTCCCTCCTGATTCTAAAATGTCTCAAGTGCAATGCCCCCTCCCCTCCTTTACCGAGG
  ACAGCCTGCCTCTGCCACAGCAAGGCTGTCGGGGTCAAGCTGGAAAGGCCAGCAGCCTTCCAGTGGCTTC
  TCCCAACACTCTTGGGGACCAAATATATTTAATGGTTAAGGGACTTGTCCCAAGTCTGACAGCCAGAGCG
  TTAGAGGGGCCAGCGGCCCTCCCAGGCGATCTTGTGTCTACTCTAGGACTGGGCCCGAGGGTGGTTTACC
  TGCACCGTTGACTCAGTATAGTTTAAAAATCTGCCACCTGCACAGGTATTTTTGAAAGCAAAATAAGGTT
  TTCTTTTTTCCCCTTTCTTGTAATAAATGATAAAATTCCGAGTCTTTCTCACTGCCTTTGTTTAGAAGAG
  AGTAGCTCGTCCTCACTGGTCTACACTGGTTGCCGAATTTACTTGTATTCCTAACTGTTTTGTATATGCT
  GCATTGAGACTTACGGCAAGAAGGCATTTTTTTTTTTTTAAAGGAAACAAACTCTCAAATCATGAAGTGA
  TATAAAAGCTGCATATGCCTACAAAGCTCTGAATTCAGGTCCCAGTTGCTGTCACAAAGGAGTGAGTGAA
  ACTCCCACCCTACCCCCTTTTTTATATAATAAAAGTGCCTTAGCATGTGTTGCAGCTGTCACCACTACAG
  TAATCTGGTTTACAGATGTTTTCCACTGAGCATCACAATAAAGAGAACCATGTGCTAAAAAAAAAAAAAA
  AAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK025393 unedited
  CCCAGCTCGTTTTTGTATACGACTCATATAGGCGGCCTGCGAATCGGCACCAGGTCGGCC
  GANAAAACTGAAGGAGTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTAC
  AAGGACCAGGCCGACAAGGCATCTACCCGCCTGAAGCAGCTCAAGCGGCAGCTGGAGGAG
  GCTCGAAGAGGAGGCCCAGCGGGCCAACGCCTCCCGCCGGAAACTGCAGCGCGAGCTGGA
  GGACGCCACTGAGACGGCCGATGCCATGAACCGCGAAGTCAGCTCCCTAAAGAACAAGCT
  CAGGCGCGGGGACCTGCCGTTTGTCGTGCCCCGCCGAATGGCCCGGAAAGGCGCCGGGGA
  TGGCTCCGACGAAGAGGTAGATGGCAAAGCGGATGGGGCTGAGGCCAAACCTGCCGAATA
  AGCCTCTTCTCCTGCAGCCTGAGATGGATGGACAGACAGACACCACAGCCTCCCCTTCCC
  AGACCCCGCAGCACGCCTCTCCCCACCTTCTTGGGACTGCTGTGAACATGCCTCCTCCTG
  CCCTCCGCCCCGTCCCCCCATCCCGTTTCCCTCCAGGTGTTGTTGAGGGCATTTGGCTTC
  CTCTGCTGCATCCCCTTCCAGCTCCCTCCCCTGCTCAGAATCTGATACCAAAGAGACATG
  GCCCGGGCCCAGGCAGAGAGCCACCAGCAGGCTCCTCAGCCCTCTCTTGCCAAAAGCACA
  AGATGTTGAGGCGAGCAGGGCAGGCCCCCGGNGAGGGGCTCAGAGNTTTCTATGAATCTA
  TGTTTCTTCAGACTGAGGCCTNTTTGGTAGTCGNAGCCCCCGCAGTCGTCAGCCTCCCTG
  ACGTATGGCAGCAGNGCCCNCACTCTCCTNCTTTTTTTGCTGATCCAACACCACGTGGGG
  ACTNACACACCTCTGCCCATGGGCCTNCCACTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK025393 unedited
  GGTCCTCTATGNACGCGGCCGCCTTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTAGCACATGGGTTCTCTTTATTGGGATGCTCAGTGGAAAACATCTGTAAACCAG
  CTTACTGTAGTGGTGACAGCTACAACACATGCTAAGGCACTTTTATTATATAAAAAAGGG
  GGTAGGGTGGGAGTTTCACTCACTCCTTTGTGACAGCAACTGGGACCTGAATTCAAAGCT
  TTGTAGGCATATGCAGCTTTTATATCACTTCATGATTTGAGAGTTTGTTTCCTTTAAAAA
  AAAAAAATGCCTTCTTGCCGTAAGTCTCAATGCAGCATATACAAAACAGTTAGGAATACA
  AGTAAATTCGGCAACCAGTGTAGACCAGTGAGGACGAGCTACTCTCTTCTAAACAAAGGC
  AGTGAGAAAGACTCGGAATTTTATCATTTATTACAAGAAAGGGGAAAAAAGAAAACCTTA
  TTTTGCTTTCAAAAATACCTGTGCAGGTGGCAGATTTTTAAACTATACTGAGTCAACGGT
  GCAGGTAAACCACCCTCGGGCCCAGTCCTAGAGTAGACACAAGATCGCCTGGGAGGGCCG
  CTGGCCCCTCTAACGCTCTGGCTGTCAGACTTGGGACAAGTCCCTTAACCATTAAATATA
  TTTTGTCCCCAAGAGTGTTGGGAGAAGCCACTGGGAGGCTGCTGGCCTTTCCAGCTTGAC
  CCCAACANCCTTGCTGTGGCAAAAGCAGGCTGTCCCGGGTAAAAGGAAGGGAGGGGGCAT
  GCACTTTGAAACTTTTTAGAATCCAGAGGGAGACCCCGGACAGTGGCGCTGCCTGGGACA
  GGGGGGGGAAGGAATCCATCCTCCCCCACCCCGACTTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: AK025393
• Insert Size: 1850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK025393.1
• RefSeq Size: 1968 bp
• RefSeq ORF: 1968 bp
• Locus ID: 4627
• Cytogenetics: 22q12.3
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton, Tight junction, Viral myocarditis
• Gene Summary: 'This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]'