MTAP (AF109294) Human Untagged Clone

CAT#: SC105031

(untagged)-Human hypothetical methylthioadenosine phosphorylase fusion protein mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTAP
• Synonyms: BDMF|DMSFH|DMSMFH|HEL-249|LGMBF|MSAP|c86fus
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF109294, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTGGATGATCCAG
  AAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAGCCATCTGATGCCTTAATTTT
  GGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGGCATGGAAGGCAGCACACCATCATGCCTTCA
  AAGGTCAACTACCAGGCGAACATCTGGGCTTTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAG
  CTTGTGGCTCCTTGAGGGAGGAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGAC
  TATTTGCCACGACATTTCAAAGGATTCCAAGAGAGAATATTGGTGTCCATGCTGTGATGATTCCTCAGCT
  CCTCTCATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF109294 unedited
  NNCCGTTCGAATTTTGTATACGTACTTCACTATAGGGCGGCCGCGAATTCGCACCAGNAC
  CACCGCTCTGTGGCTCGCTTGGTTCCCTTAGTCCCGAGCGCTCGCCCACTGCAGATTCCT
  TTCCCGTGCAGACATGGCCTCTGGCACCACCACCACCGCCGTGAAGGTGAGATGAGCCCT
  CCCAGCCGCAGCGGTTCGCCCTGCCGGATGCCTTCTCGCCCCCGCGCCGGGGGACCGCGC
  CTCCGGGGGCCATGCGCCCGGCCCGTGCGTCCCTTGCCGCCGCGGGGAGGGACTGGGGCG
  CGGCACTCGGGACTCACTTGCCGCGCGAGGAGAGGATTGGAATAATTGGTGGAACAGGCC
  TGGATGATCCAGAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCA
  AGCCATCTGATGCCTTAATTTTGGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAA
  GGCATGGAAGGCAGCACACCATCATGCCTTCAAAGGTCAACTACCAGGCGAACATCTGGG
  CTTTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAGCTTGTGGCTCCTTGAGGG
  AGGAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGACCACTATGA
  GACCTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAA
  TGGCTGAGCCGTTTTGCCCCAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAG
  GACTCCGGTGCCACTCAAAGGGGACAATGGTCACCATCGAGGGACCTCGGTTTTAGCTCC
  CGGGCAGAAAGCTTCATGTTTCGCACCCTGGGGGCGGGATGTTATCAACTGGACCCCATT
  CCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: AF109294
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF109294.1, AAD19641.1
• RefSeq Size: 942 bp
• RefSeq ORF: 942 bp
• Locus ID: 4507
• Cytogenetics: 9p21.3
• Domains: Mtap_PNP
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]'