UBE2D2 (AK001428) Human Untagged Clone

CAT#: SC105115

(untagged)-Human cDNA FLJ10566 fis, clone NT2RP2002959, highly similar to UBIQUITIN-CONJUGATING ENZYME E2-17 KD 2 (EC 6.3.2.19)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2D2
• Synonyms: 5|UBCH4|UBCH5B; E2(17)KB2|PUBC1|UBC4|UBC4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC105115 sequence for AK001428 edited (data generated by NextGen Sequencing)
  ATGGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAG
  TGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCA
  AATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTAC
  CCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGT
  AATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCA
  AAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTG
  CCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAA
  TGGACTCAGAAGTATGCGATGTAA
  
  Clone variation with respect to AK001428.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK001428 unedited
  TGGATTTTGTAATACGACTTTACTATAGGGCGGCCGCGAATTCGCACGAGAGCAGGGAGG
  AAGACAGGCAATCCCTCCGGCTGTCCGACCAAGAGAGGCCGGCCGAGCCCGAGGCTTGGG
  CTTTTGCTTTCTGGCGGAGGGATCTGCGGCGGTTTAGGAGGCGGCGCTGATCCTGGGAGG
  AAGAGGCAGCTACGGCGGCGGCGGCGGTGGCGGCTAGGGCGGCGGCGAATAAAGGGGCCG
  CCGCCGGGTGATGCGGTGACCGCTGCGGCAGGCCCAGGAGCTGAGTGGGCCCCGGCCCTC
  AGCCCGTCCCGCCGGACCCGCTTTCCTCAACTCTCCATCTTCTCCTGCCGACCGAGATCG
  CCGAGGCGGCCTCAGGCTCCCTAGCCCCTTCCCCGTCCCTTCCCCGCCCCCGTCCCCGCC
  CCGGGGGCCGCCGCCACCCGCCTCCCACCATGGCTCTGAAGAGAATCCACAAGGAATTGA
  ATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGT
  TCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTT
  TCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAA
  CAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGAT
  CACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGT
  GTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTTGCTCGATCTNCAAACAGATGA
  GAAAAGTCCACAGATAGCTCGGGAATGACTCAGAGTATGCGATGTATTAAAGAAATATGN
  ATACCTCTACAATAAGATAGGGGACTCTGAAGAGAAGTCCTTTGATTCCATTGACTGCTT
  CTTGAGCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: AK001428
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK001428.1, BAG50911.1
• RefSeq Size: 2526 bp
• RefSeq ORF: 2526 bp
• Locus ID: 7322
• Cytogenetics: 5q31.2
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]'