GRF2 (RAPGEF1) (BD140654) Human Untagged Clone

CAT#: SC105577

(untagged)-36 Human secreted proteins

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAPGEF1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BD140654, the custom clone sequence may differ by one or more nucleotides
  
  
  GCCCTTTACCCCCAACCCCAGGCCACTGGGCCCTTCCCACACCACTTGGGGAGCTGAGAAGAGGAGGCTG
  GAGTAAGGGAGGACTTGATCATCCAAGAAATACTTTTTATTGCTGGGAGTCTTCTGAACCTCACCAAACT
  GAGGCCAGAGCTGAGCTCCTGGGGGAGTTAATTCAGAGGGGAGAGGCCAGCACCTCCCTCCTCCATYGCT
  CGCTGTGTGCCTTAAACTCCATCTCATGTCCCTCCCCATCCCCTGGCTTTCCCTCCCTCCTTGCCCCATC
  CTGGGCCAGCCAGCAGGGCTCCTCCTCTGGCTCTTCAGACCTTTCAGCCAGTGCTGTCAGTGCCCCTGGG
  AGGGAAGGGCATCCCTGAGGCACCCGAATGGTCCCTCAGGGTGCAGGGAGGCAGAAGCCTGGCCACAGAG
  GAGCCTCCTAAGGCAGCAGCTGCAGCAAGCGCACCCTCTCCCCACTCTCCCCACGCCAGAGCGGCTTCCA
  GAGCAGATGCTGTTTCCATCCTCCTCGTCAAAACCATTCTCGCTGCTGAGCTTGACAATCTGGGCAAGGC
  TTGTGGGGCGCTTGACAAACAGAATCTGCCCTGTGCCGCCTGGTTCCGTGGCCTCCAGCATGAGCCTGCA
  GGCAGGGCGCTGCGGGAACCCAGTTGTGCTGCCCCAGCCCATGCCTCCGGGTCTGCTGTGCATGAATGAG
  TGCTCACTTGTCCCGGGTTTAGGACGTGGTCAAGTGAACAGCAGGGTCTAACTGTGCTTACTTAGCCCAG
  TTCAAACAGAACAAAGGAAAAATATAGAAAGCAACATCTGTTGATCATTTAGGTTTTTTTTTAAACCACC
  ATGTCACTTTGAGTCCTTCATGGGTTTTTGAACAGCATTTATCAAGAAGAAAATGTGGGCTTTTTCCCCT
  CTCCCGTGTTTTGTTTGTCCTGTAGATAGAGGGAGGAAAGCCGTGCAGTGGCAGGCGGGACCCCCTCTGG
  TGGCGGGACCCCCTCTTGCGGTGGTCTTGCGGGGCCAGCCGGGACCTGTCACTTTATTATTTAAGGAGTG
  TGTGTGTAGAGTCGCTGGCTTATTAACAGTATTGTGTGTGGGTTGGGTTTTTAGTTTGTTCCTTCTTTTT
  GAAGTCCCTTCATTTCAATCCTTGACTCTCTCTCCCCTTCCCTTGCCCAGCTCTGTTGAATGCTGCTGTG
  CGCGTGTGAGGGCCGCTCTGCACACAGGGCCCTTGGGTTGTGTGAACTGAAATTCTCCCTGTATTTGTGA
  GACTCGCAGGAGTCCCCATCTGTAGCACAGGCAATGCCAGTGCCATGCTGCAGCCTCAGAAACCAGGCCT
  CTCACTCCAGCAGCAGGCAGAACCGTGTCTGTGGTCGGGTGCTGTCCACAGCTCTGTCTGCCTTGTTCTT
  GGGCTTGAGCTGGATAGAGGTGGGGTCTCTTCACCTTCCCTGAATTCAGAACAGACCCTGTGCCTGGCCC
  CAGTGTGCCCAGGCAATTCCCCAGGCCCTCATTGGGAGCCCTTGGTGTTCTGAGCAGCAGGGCCCAGGCA
  GCACATGAGCAGTGCCCAGGGGCTCCCTGCGTGAGGACGGCAAGGTGCGATGTATGTCTAACTTATTGAT
  GGCAGGCAGCCCCCTGTGCCCCCTAAGCCTGGCCCTGGTTATTGCTGAGCTCTGTGCTCAGTGCTGCGGC
  CTGGCCGTGGCTCGTCTGTTCCTTTGGGGGGCCCGGGCGGGTTGTGGGAATCAGTCTTCACAGACAGACG
  TGAGCCAGGCGGAGGACTCGTTCCTTGCAGAGGTCAGTCCTCACCTGCAGGTGTCGGGGTGGRGGGGGGC
  AAGGAGGGGCAGGCACACACCATGTCTGACCTGAACCCGATTCTGGGGAGCATCTTCCCGCTCCGGCCCC
  ACGACCTCCACAGGGTTACATTGTAATATATATGCCCCAGCTAACCTGTCTGATGGTGGCATCTTCCTGC
  AGACATTTCAAACATGTAACTTTTATATGAAAAAAAATAAACACAGATGAAAGCTGCCCAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BD140654 unedited
  GGTGCAGCATTTGTAACCGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCAGCACCT
  CCCTCCTCCATCGCTCGCTGTGTGCCTTAAACTCCATCTCATGTCCCTCCCCATCCCCTG
  GCTTTCCCTCCCTCCTTGCCCCATCCTGGGCCAGCCAGCAGGGCTCCTCCTCTGGCTCTT
  CAGACCTTTCAGCCAGTGCTGTCAGTGCCCCTGGGAGGGAAGGGCATCCCTGAGGCACCC
  GAATGGTCCCTCAGGGTGCAGGGAGGCAGAAGCCTGGCCACAGAGGAGCCTCCTAAGGCA
  GCAGCTGCAGCAAGCGCACCCTCTCCCCACTCTCCCCACGCCAGAGCGGCTTCCAGAGCA
  GATGCTGTTTCCATCCTCCTCGTCAAAACCATTCTCGCTGCTGAGCTTGACAATCTGGGC
  AAGGCTTGTGGGGCGCTTGACAAACAGAATCTGCCCTGTGCCGCCTGGTTCCGTGGCCTC
  CAGCATGAGCCTGCAGGCAGGGCGCTGCGGGAACCCAGTTGTGCTGCCCCAGCCCATGCC
  TCCGGGTCTGCTGTGCATGAATGAGTGCTCACTTGTCCCGGGTTTAGGACGTGGTCAAGT
  GAACAGCAGGGTCTAACTGTGCTTACTTAGCCCAGTTCANACAGAACANAGGANAATATA
  GAAAGCAACATCTGTTGATCATTTAGGTTTTTTTTTAAACCACCATGTCACTTTGAGTCC
  TTCATGGGTTTTTGAACAGCATTTATCAAGAAGAAAAATGTGGGCTTTTTTCCCTCTCCC
  GTGTTTTGTTTGTCCTGGTANATAGAGGAAGGAAGGCGTTGCCATGGCAGGGCGGGACCC
  CCTCTGTGGCGGGCACCCCTCTTGGCGGGGTCTTGCGGGGCCGCCCGGACCTGTCCCTTA
  TTTTTAAGGATGTGGGGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BD140654 unedited
  NTTAGGTACACTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTGG
  GCAGCTTTCATCTGTGTTTATTTTTTTTCATATAAAAGTTACATGTTCTGAAATGTCTGC
  AGGAAGATGCCACCATCAGACAGGTTAGCTGGGGCATATATATTACAATGTAACCCTGTG
  GAGGTCGTGGGGCCGGAGCGGGAAGATGCTCCCCAGAATCGGGTTCAGGTCAGACATGGT
  GTGTGCCTGCCCCTCCTTGCCCCCCCCCACCCCGACACCTGCAGGTGAGGACTGACCTCT
  GCAAGGAACGAGTCCTCCGCCTGGCTCACGTCTGTCTGTGAAGACTGATTCCCACAACCC
  GCCCGGGCCCCCCAAAGGAACAGACGAGCCACGGCCAGGCCGCAGCACTGAGCACAGAGC
  TCAGCAATAACCAGGGCCAGGCTTATGGGGCACAGGGGGCTGCCTGCCATCAATAAGTTA
  GACATACATCGCACCTTGCCGTCCTCACGCAGGGAGCCCCTGGGCACTGCTCATGTGCTG
  CCTGGGCCCTGCTGCTCAGAACACCAAGGGGCTCCCAATGATGGCCTGGGGAATTGCCTG
  GGCACACTGGGGGCCAGCACAGGGTCTGTTCTGAATTCAGGGAAGGTGAAGAGACCCCAC
  CTCTATCCAGCTCAAGCCCAAGAACAAGGCAGACAGAGCTGTGGACAGCACCCGACCACA
  GACACGGTTCTGCCTGCTGCTGGAGTGAGAAGCCTGGGTTTCTGAGCTGCAGCATGGCAC
  TGGCATTGCCTGTGCTACAGATGGGGACTCCTGCGAGTCTCACAAATACAGGAGAATTTC
  AGTTCACACAACCCAAGGGCCCTGTGTGCAAC
  
• Restriction Sites: NotI-NotI
• ACCN: BD140654
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BD140654.1
• RefSeq Size: 2083 bp
• RefSeq ORF: 2083 bp
• Locus ID: 2889
• Cytogenetics: 9q34.13
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
• Gene Summary: 'This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]'