MEG3 (AF052114) Human Untagged Clone

CAT#: SC105816

(untagged)-Human clone 23887 mRNA sequence


Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MEG3
Synonyms FP504|GTL2|LINC00023|NCRNA00023|PRO0518|PRO2160|onco-lncRNA-83|prebp1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for AF052114, the custom clone sequence may differ by one or more nucleotides


ACAAAGCAGCCACTCACTGACCCCCAGGACCAGGATGGCAAAGGATGAAGAGGACCGGAACTGACCAGCC
AGCTGTCCCTCTTACCTAAAGACTTAAACCAATGCCCTAGTGAGGGGGCATTGGGCATTAAGCCCTGACC
TTTGCTATGCTCATACTTTGACTCTATGAGTACTTTCCTATAAGTCTTTGCTTGTGTTCACCTGCTAGCA
AACTGGAGTGTTTCCCTCCCCAAGGGGGTGTCAGTCTTTGTCGACTGACTCTGTCATCACCCTTATGATG
TCCTGAATGGAAGGATCCCTTTGGGAAATTCTCAGGAGGGGGACCTGGGCCAAGGGCTTGGCCAGCATCC
TGCTGGCAACTCCAAGGCCCTGGGTGGGCTTCTGGAATGAGCATGCTACTGAATCACCAAAGGCACGCCC
GACCTCTCTGAAGATCTTCCTATCCTTTTCTGGGGGAATGGGGTCGATGAGAGCAACCTCCTAGGGTTGT
TGTGAGAATTAAATGAGATAAAAGAGGCCTCAGGCAGGATCTGGCATAGAGGAGGTGATCAGCAAATGTT
TGTTGAAAAGGTTTGACAGGTCAGTCCCTTCCCACCCCTCTTGCTTGTCTTACTTGTCTTATTTATTCTC
CAACAGCACTCCAGGCAGCCCTTGTCCACGGGCTCTCCTTGCATCAGCCAAGCTTCTTGAAAGGCCTGTC
TACACTTGCTGTCTTCCTTCCTCACCTCCAATTTCCTCTTCAACCCACTGCTTCCTGACTCGCTCTACTC
CGTGGAAGCACGCTCACAAAGGCACGTGGGCCGTGGCCCGGCTGGGTCGGCTGAAGAACTGCGGATGGAA
GCTGCGGAAGAGGCCCTGATGGGGCCCACCATCCCGGACCCAAGTCTTCTTCCTGGCGGGCCTCTCGTCT
CCTTCCTGGTTTGGGCGGAAGCCATCACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCCCA
GCCCCTCTCCAGCTCGAAATCGGCAGACTAGGATGGAAGTGCCCTGTGAGCTGGGGGGCCCTTCAAAGGG
CCAAGGAGAAAACGCAGGCCGAGGGACCAGCCTTCCAAATGGGCTTCAAGCTCCAATGACCTCCGCTCGC
CCCCTCGAAATGTCTGGAAAACATAATGGGCAGATTTTCTGTCTTCAAAGTTTCCGGCTAAACCTCTTCA
AGTTCTTTATTGTTTGGGACTGAGACACTCAGCCATGTTAATGGGTAGTTTCTTTTGTATTTGCCTTGAA
AGGCCAAAATATTTTTATATTGCCACAGACAAAGCCACCTATTTAAAAATGAACTCCATGTCCGTCGTTT
CCCACCAGGAGACTATGTACCATGTGTGTGTCTCTATGTATTCTGGGGTCTTGAAACAGGTTTCTCATGG
GGATGGTCATTCACCACGGTCCAGAGGGGCAGAACAGGCGGCGCTTGCCTTGCCCAGGGGGCCTGGGGAA
CGTGGGCCCTCATCTCAGATCTGCCCCCAGTATGTTTAGGACGCGAGCCCCAGAAGGATCTGGGAGTAAA
CTTAACATTCACTGTGTCTCTGCTCTGCATCCGCCATTTGTGTGTGTTTCTGGACTGTGGGCTGTGTGTA
CCTTGGTTGGTGACTCAGTGAGAAGAAGCAGGAATGCCAAAGATACTGTGAATGTTCTGAGTTTTGTTGC
TGTTGTTGTTGAGAGGTTGTTTCACTGGTATCTATTGCATTGTATAATAAATGACCAGATGAATGAATGA
GTGAAGCAAGAGAGAATGAATAAACAAGTAAATAGGTAAAGAAGTAAGCAAGCCAGGATGAGAGTGTGTG
TACACAAGACCATGGTTCATCCGCTTTGATGGCTAGGCAATCAATATATAAATAGAAAAAAACCAGTGAA
TCACTAAGTAATAGGGCAACACACAAAGCGATATCAGGTGATTATGGACTAAGGGGTATGTGTAACTCAA
ATATATGCCTCTGACATTTGACAATGAAAAAGAACCTAAATGAAAGAAAGAATGGATGTATGAGTAGTGA
AGTGCAGAATGAGACATAGATTTTGAGGCCCGTCAAAATGAAAAGATGCAAGTTAGGGAACAAGTGATCA
AAAGGGAGAAGGGAAAGGTTTTTTTTAAAAAACCAAAACAACAAAGAAAGGTTTAAAAAAAAAACAGACT
AGAGGATGAGTAATGAGTAACTCTGTAAGGAGGACCATGTCAGACTATTGTAAGCTAAGCATTAGGACTG
ATACAAATAATATATGCTCCTGGCATAGAAAAATAAACCACAGAGAACGAGTTCAAAGAATAGCAAAGAA
AGAAAGAGGACCCAGTGGGCGAAAGATGAGAGTGTACTTTTACCAAAAGTTATCTAAGCCTGAGCACTTG
AAGTCTGCACATAAATAAATAAATGACAAAAGAAAGAAAAAAAAAAAAAAAAA


>OriGene 5' read for AF052114 unedited
CAGGAATTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCCCGGCTG
GGTCGGCTGAAGAACTGCGGATGGAAGCTGCGGAAGAGGCCCTGATGGGGCCCACCATCC
CGGACCCAAGTCTTCTTCCTGGCGGGCCTCTCGTCTCCTTCCTGGTTTGGGCGGAAGCCA
TCACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCCCAGCCCCTCTCCAGCT
CGAAATCGGCAGACTAGGATGGAAGTGCCCTGTGAGCTGGGGGGCCCTTCAAAGGGCCAA
GGAGAAAACGCAGGCCGAGGGACCAGCCTTCCAAATGGGCTTCAAGCTCCAATGACCTCC
GCTCGCCCCCTCGAAATGTCTGGAAAACATAATGGGCAGATTTTCTGTCTTCAAAGTTTC
CGGCTAAACCTCTTCAAGTTCTTTATTGTTTGGGACTGAGACACTCAGCCATGTTAATGG
GTAGTTTCTTTTGTATTTGCCTTGAAAGGCCAAAATATTTTTATATTGCCACAGACAAAG
CCACCTATTTAAAAATGAACTCCATGTCCGTCGTTTCCCACCAGGAGACTATGTACCATG
TGTGTGTCTCTATGTATTCTGGGGTCTTGAAACAGGTTTCTCATGGGGATGGTCATTCAC
CACGGTCCAGAGGGGCAGAACAGGCGGCGCTTGCCTTGCCCAGNGGGCCTGGGGAAACGT
GGCCCTCATCTCAGATCTGCCCCCAGTATGTTTAGGACGCGAGCCCCAGAAGGATCTGNG
AGTAAACTTACATTCACTGTGTCTCTGCTCTGCATCCGCCATTTNNGTGTGTGTTCTGGA
CTGTGGGCTGTGTGTACCTTGGTGGTGACTCAGTGAGAAGAAGCAGATGCCAAGAACTGT
GATGTTCTGAGTTTTGTGCTGTGTGTTGAAAGGTGNTCACTGGATCTATTGCATGNATAT
AAGGACGATGAATGATGT
>OriGene 3' read for AF052114 unedited
NGGGTACAGCTATGNACCGCGGCACGCATNCTAGAGATCGAGTTTTTTTTTTTTTTTTTT
GTTGTTTTGGTTTTTTAAAAAAAACTTTCCCTTCCCCTTTTGAACACTTGTTCCCTAACT
TGCATCTTTTCATTTTGACGGGCCTCAAAATCTATGTCTCATTCTGCACTTCACTACTCA
TACATCCATTCTTTCTTTCATTTAAGTTCTTTTTCATTGTCAAATGTCAGAGGCATATAT
TTGAGTTACACATACCCCTTAGTCCATAATCACCTGATATCGCTTTGTGTGTTGCCCTAT
TACTTAGTGATTCACTGGTTTTTTTCTATTTATATATTGATTGCCTAGCCATCAAAGCGG
ATGAACCATGGTCTTGTGTACACACACTCTCATCCTGGCTTGCTTACTTCTTTACCTATT
TACTTGTTTATTCATTCTCTCTTGCTTCACTCATTCATTCATCTGGTCATTTATTATACA
ATGCAATAGATACCAGTGAAACAACCTCTCAACAACAACAGCAACAAAACTCAGAACATT
CACAGTATCTTTGGCATTCCTGCTTCTTCTCACTGAGTCACCAACCAAGGTACACACAGC
CCACAGTCCAGAAACACACACAAATGGCGGATGCAGAGCAGAGACACAGTGAATGTTAAG
TTTACTCCCAGATCCTTCTGGGGCTCGCGTCCTAAACATACTGGGGGCAGATCTGAGATG
AGGGCCCACGTTCCCCCAGCCCCCTGGGCAAGGCAAGCGCCGCCTGTTCTGCCCCTCTGG
ACCGTGGTGAATGACCATCCCCATGAAGAAACTGTTTTCAAGACCCCCGNATACATAGAG
ACACACACTGGTACATAGTCTCCCGGTGGGGAACGACGGACATGNAGTCCAT
Restriction Sites NotI-NotI     
ACCN AF052114
ORF Size 2433 bp
Insert Size 1400
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq AF052114.1
RefSeq Size 2433
RefSeq ORF 2433
Locus ID 55384
Gene Summary This gene is a maternally expressed imprinted gene. Multiple alternatively spliced transcript variants have been transcribed from this gene and all of them are long non-coding RNAs (lncRNAs). This gene is expressed in many normal tissues, but its expression is lost in multiple cancer cell lines of various tissue origins. It inhibits tumor cell proliferation in vitro. It also interacts with the tumor suppressor p53, and regulates p53 target gene expression. Its deletion enhances angiogenesis in vivo. Many experimental evidences demonstrate that this gene is a lncRNA tumor suppressor. [provided by RefSeq, Mar 2012]

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