ZNF148 (BC018971) Human Untagged Clone

CAT#: SC106061

ZNF148 (untagged)-Homo sapiens, clone IMAGE:4336243

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZNF148
• Synonyms: BERF-1; BFCOL1; CACCC box-binding protein; CLL-associated antigen KW-10; HT-BETA; pHZ-52; ZBP-89; ZFP148; zinc finger protein 148
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC018971, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGTTTCATACAGAAGTACCTGCTTCAGAGACATGAGAAGATTCATACTGGTGAAAAACCATTTCGCT
  GTGATGAATGTGGTGATGAGACAAACCATGCTTCTGCCACATCAACACAGGACTTTCTGGATCAAGTGAC
  TTCTCAGAAGAAAGCTGAGGCCCAGCCTGTCCACCAAGCTTACCAAATGAGCTCCTTTGAACAGCCCTTC
  CGTGCTCCCTATCATGGATCAAGAGCTGGAATAGCTACTCAATTTAGCACTGCCAATGGACAGGTGAACC
  TTCGGGGACCAGGGACAAGTGCTGAATTTTCAGAATTTCCCTTGGTGAATGTAAATGATAATAGAGCTGG
  GATGACATCTTCACCTGATGCCACAACTGGCCAGACTTTTGGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC018971 unedited
  GTGCCCATTTGTATACGACTCCTATAGGCGGCCGCGATTCGCCGAGGAAAGCGCAGGGTG
  GGAGCTGTTGCCGAAGCTGCCACAGCAAAAGTTCTCCCCCCTCCCCCCTTCCCCTCCTCT
  CAAGGCCCCTAGAAAGGTTGGAGCTGCCGCCGCCTGCAGTCGGTGACCGCGCGACTCGGC
  GCCCGCCCGCGGATAGAGGGAGGAATCAGCAGCTTGGAAATTCAAGCACGTGATCTGGCG
  GGATGGGCGTTTGCCTAACGTATTTAATGGAGGAATCGGATGGCATAAGTGATTAAGGTG
  GTATTGAGGATTTCTGAAGCCTATGAAAGGTAGAAACTCAACCATGATTTCTTTTTCAAC
  TCTACAGCATTCCTTTCCTTGAAGTCTTCGTTTTTACCTTAGTCTCGGGCAGTTATACTT
  AAGCATGAACATTGACGACAAACTGGAAGGATTGTTTCTTAAATGTGGCGGCATAGACGA
  AATGCAGTCTTCCAGGACAATGGTTGTAATGGGTGGAGTGTCTGGCCAGTCTACTGTGTC
  TGGAGAGCTACAGGATTCAGTACTTCAAGATCGAAGTATGCCTCACCAGGAGATCCTTGC
  TGCAGATGAAGTGTTACAAGAAAGTGAAATGAGACAACAGGATATGATATCACATGATGA
  ACTCATGGTCCATGANGAGACAGTGAAAAATGATGAAGAGCAGATGGAAACACATGANAG
  ACTTCCTCAAGGACTACAGTATGCACTTAATGTCCCTATNAGCGNTAAGCANGAAATTAC
  TTTTACTGATGTATCTGAGCAACTGATGAGAGACAAAAACCAATTAGAGAGCCAGTAGAC
  TTACAGAAAAAAGAGAAAGCGAAACCACGTCCTCCCGCAAAATCCTTACAATAATGAGGA
  TGGATCACTTGGTTTGGAAACCCCTAATCTTACTTTG
  
• Restriction Sites: NotI-NotI
• ACCN: BC018971
• Insert Size: 4600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018971.1
• RefSeq Size: 1034 bp
• RefSeq ORF: 832 bp
• Locus ID: 7707
• Cytogenetics: 3q21.2
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies. [provided by RefSeq, Feb 2017]'