Iduronate 2 sulfatase (IDS) (AF050145) Human Untagged Clone

CAT#: SC106082

(untagged)-Human iduronate-2-sulfatase (IDS) mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IDS
• Synonyms: MPS2|SIDS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF050145, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGCCACCCCGGACCGGCCGAGGCCTTCTCTGGCTGGGTCTGGTTCTGAGCTCCGTCTGCGTCGCCC
  TCGGATCCGAAACGCAGGCCAACTCGACCACAGATGCTCTGAACGTTCTTCTCATCATCGTGGATGACCT
  GCGCCCCTCCCTGGGCTGTTATGGGGATAAGCTGGTGAGGTCCCCAAATATTGACCAACTGGCATCCCAC
  AGCCTCCTCTTCCAGAATGCCTTTGCGCAGCAAGCAGTGTGCGCCCCGAGCCGCGTTTCTTTCCTCACTG
  GCAGGAGACCTGACACCACCCGCCTGTACGACTTCAACTCCTACTGGAGGGTGCACGCTGGAAACTTCTC
  CACCATCCCCCAGTACTTCAAGGAGAATGGCTATGTGACCATGTCGGTGGGAAAAGTCTTTCACCCTGGT
  ACTGCTCCATGTCCAGAGTCTGGGTTCTCTTGGTTTGTGGTGTCTGAATCCAGCATTCCCATCCTGGGGA
  TGGGGCTGTCTTTGCAGAGCCCTCTTCTGGCTGGGCGAGTCCCTCGCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF050145 unedited
  GGGCGGCCCGCGAATTCGCACGAGGGTCGCACGGACGCACTCGCGCTGCGGCCAGCGCCC
  GGGCCTGCGGGCCCGGGCGGCGGCTGTGTTGCGCAGTCTTCATGGGTTCCCGACGAGGAG
  GTCTCTGTGGCTGCGGCGGCGGCTGCTAACTGCGCCACCTGCTGCAGCCTGTCCCCGCCG
  CTCTGAAGCGGCCGCGTCGAAGCCGAAATGCCGCCACCCCGGACCGGCCGAGGCCTTCTC
  TGGCTGGGTCTGGTTCTGAGCTCCGTCTGCGTCGCCCTCGGATCCGAAACGCAGGCCAAC
  TCGACCACAGATGCTCTGAACGTTCTTCTCATCATCGTGGATGACCTGCGCCCCTCCCTG
  GGCTGTTATGGGGATAAGCTGGTGAGGTCCCCAAATATTGACCAACTGGCATCCCACAGC
  CTCCTCTTCCAGAATGCCTTTGCGCAGCAAGCAGTGTGCGCCCCGAGCCGCGTTTCTTTC
  CTCACTGGCAGGAGACCTGACACCACCCGCCTGTACGACTTCAACTCCTACTGGAGGGTG
  CACGCTGGAAACTTCTCCACCATCCCCCAGTACTTCAAGGAGAATGGCTATGTGACCATG
  TCGGTGGGAAAAGTCTTTCACCCTGAGATATCTTCTAACCATACCGATGATTCTCCGTAT
  AGCTAGTCTTTTCCACCTTATCATCCTTCCTCTGAGAGTATGAAACACTAAAACTGTCGA
  GGCCAGATGAGAACTCCTGCAACCTGCTTGCCCTGGGATGGCTGGATGTCCCGAGCCACT
  TGCCTGACAACAGACACTGAGCAGCCATACAGTGTTGGAAAAAAGAAACGTNACCAGTCC
  TTTCTTCTGCCCGTGGTATATAAGCACCCTCCCTTCAGAACCCAAGAN
  
• Restriction Sites: NotI-NotI
• ACCN: AF050145
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF050145.1, AAC05984.1
• RefSeq Size: 1098 bp
• RefSeq ORF: 1098 bp
• Locus ID: 3423
• Cytogenetics: Xq28
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]'