PDE6 beta (PDE6B) (BC020343) Human Untagged Clone

CAT#: SC106083

(untagged)-Homo sapiens, clone IMAGE:4157950

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE6B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC020343, the custom clone sequence may differ by one or more nucleotides
  
  
  CAGGGGGTTCAGGAGGCGAGGAGGCCAGGGCGTGGGTTGGAGGACGGCTGTGCTGCCTTCTTCCTCCTCC
  CCAGGCTGCCGTGCTGGGGCCCCTCCCTCTTGGGAACCCCTCCCTCTCCGGAGGCCGTATGGCTTCAGCC
  TGCTTTCTAGGACTCAGCGTTGGTGCCTGCCCTGCCACCCTGACCCTGACTTGTGCTCAAGCCTGGCACC
  TGGATCCTGGTTCCTGCTTGGGGCCTGGGGATGGGGTCCCAATAGGCTTATGGGATCACAGGGCCACTGG
  GCCACCAGCTGCCCCTCCCTTGGCTGGAGTCCTGCCCTGACCTCCGGACTGGAGTTCCCCTCCTCTTCCA
  GCCCCACGGGGCCAAGACTCGGCCTCCTGGCTGGAGGCTCCACTGTCTGGGTGGGGCTGGTGGTGCCAGG
  GTCAAATCCTGGGGCCTTCATGCTCAATTGTTGCTGACGAGGCCGCCTGGACAGCTTGCCTGGTCCCCTG
  TATGTCCACTCTGGATGTCCACAGTGACTCGGGCTCCTCAGCAGCCCACGTCCTCCTCACCATCTCCCAG
  AACATTGGAGTCAGGGAAACAGAGGCGTGTGGACAGGCCCTGGGCCCCTCCCCTAAGACTCACCCGGCCA
  TCAGGCGTGCGTGGGCCCGAGTACGGCTGGGACTCTCCCATCAGCACAGACCACACGTCAAAAAATTCCT
  GGGCAGAGAAGAGGAGGCCCACGGAGTACCGCTCGCAGTTGAGGTAGGCCCGCACCGTGTAGAAGGCCTT
  GTGGAACTGCCTCTCGATGTCCGTCAGCTCCTCAAACACCTTGTTGGCCGACCACAGCAGCACCTGGAGG
  GAGGGGCACACCTGTGGGCCTGTGGCCACTCCCACCCTCACGCCGGTCTGACCACAGCAGCAGGGGTTTA
  ACTCAAGCCTGACACAGGTTTCATCTGAGCTTGTGGGGCCCGGGAGCTGGCTGGCGGTGAGGCCTCGAGG
  CTGCGGTCGTGTCCCTGATCACTCAGGAGTGGACGAGTGGAAAGTGGAATCTGTCGCTTTGAGCTTGACA
  GGCAAACATCCGACCAGCCCTTCCTCCGCCCGGCTGCTCCAGAACCACCTGCGGCGAGCAGGGCTTTGAG
  AACATCGGGCCGTGGTTTCCGCACGTCAGGCCGCCTGTGGCCTCCGCCAAGCGTCTGAGAGTGAAGCATT
  GCTGACGCACTCCACGAGGTCCTGGCCAGACCCCACTCAGGACATCCCGCTGCACAGGCCCCTGGTTGCT
  GGGTCAGCGCTGCCACCAGGCTCAGACTCACCCCAGGGCCCTCCTTGGGCCACCTTTCTCTGCCTCCTTG
  GTCAAGGCCCAGACCCAGGCCCCACCAGGCCCTGCCTCCTCACACTCGCGGCGGCCGC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC020343 unedited
  TCACAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCCCTGCCACCCT
  GACCCTGACTTGTGCTCAAGCCTGGCACCTGGATCCTGGTTCCTGCTTGGGGCCTGGGGA
  TGGGGTCCCAATAGGCTTATGGGATCACAGGGCCACTGGGCCACCAGCTGCCCCTCCCTT
  GGCTGGAGTCCTGCCCTGACCTCCGGACTGGAGTTCCCCTCCTCTTCCAGCCCCACGGGG
  CCAAGACTCGGCCTCCTGGCTGGAGGCTCCACTGTCTGGGTGGGGCTGGTGGTGCCAGGG
  TCAAATCCTGGGGCCTTCATGCTCAATTGTTGCTGACGAGGCCGCCTGGACAGCTTGCCT
  GGTCCCCTGTATGTCCACTCTGGATGTCCACAGTGACTCGGGCTCCTCAGCAGCCCACGT
  CCTCCTCACCATCTCCCAGAACAGAGGCCCACGGAGTACCGCTCGCAGTTGAGGTAGGCC
  CGCACCGTGTAGAAGGCCTTGTGGAACTGCCTCTCGATGTCCGTCAGCTCCTCAAACACC
  TTGTTGGCCGACCACAGCAGCACCTGGAGGGAGGGGCACACCTGTGGGCCTGTGNGCCAC
  TCCACCCTCACGCCGGTCTGACCACAGCAGCAGGNGTTTAACTCAAGCCTGACACANGTG
  CGCTTCCTGATCTGACCACCTGCCCAGCCGTGGAGCCTGGCACAGACCCTGATTGGTGGC
  TCCGTGGCCAGGTTTCATCTGAGCTTGTGGGGCCCCGGAGCCTGCCTGCGGTGAGGCCCT
  CCAGCTGCNGTCGTGTCCCTGACACTCANGAGTGTACCAGTGGAAAGATGAATCTGTCGC
  TTTGAGCTTGACAGGNAACATTCGACCAGNCCTTTCTTCGCCCGGNTGTTTCAGAACCAC
  CTGCGGGAGCAGGGCTTTGAGAACTTCGGCCCGTGTTTCCCCCCGTCAAGCCCCCTGTTG
  GCTCGC
  
• Restriction Sites: NotI-NotI
• ACCN: BC020343
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020343.1
• RefSeq Size: 1411 bp
• RefSeq ORF: 1098 bp
• Locus ID: 5158
• Cytogenetics: 4p16.3
• Protein Families: Druggable Genome
• Gene Summary: 'Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]'