GALNS (BC018027) Human Untagged Clone

CAT#: SC106153

(untagged)-Homo sapiens, Similar to galactosamine (N-acetyl)-6-sulfate sulfatase (Morquio syndrome, mucopolysaccharidosis type IVA), clone IMAGE:4796652

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALNS
• Synonyms: GALNAC6S|GAS|GalN6S|MPS4A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC018027, the custom clone sequence may differ by one or more nucleotides
  
  
  GTCGAGGCCCCGCCCCGCAGCCCAGCCGGAAGGGCCGGCGGACGCTCGCTAGGTCGGCTCGCTGGCCGGG
  GCTCCGCGGCTCCCGTGGTTGCCATGGCGGCGGTTGTCGCGGCGACGAGGTGGTGGCAGCTGTTGCTGGT
  GCTCAGCGCCGCGGGGATGGGGGCCTCGGGCGCCCAGCAGCCCCCCAACATCCTGCTCCTGCTCATGGAC
  GACGAATCACTCCCGCCACCTCGGGCCGCCGGGTCCCCCCGCTGGGCTCGAGTCTTACCTGACGGCTGTC
  TCTGTCCTCTGGAGAATGCTGAGCGAAGTGACTGCTTTCCAGAGCACGCTCTCCAGCGCCCAGGGGTCCG
  TGTCCTGACAGCAGTAGCCGGGCCTTTCTCTGCACTGAGTGGCATCTCCCTCCTAAAGATACTTTGCTTC
  AGAATTTCATCCTTTCACTTTTGAAGTTCCTCTGGGCCAGTGTAGATACTTTGTTTCAGAATTTCTTCTT
  TTCATTGTGTAACTCCTTCTGGGCCAGCATAGCTTCGCTGGATGAGCTGGGCTCTGCAGGTGAAGCCTCC
  CTGTTAAGTTTCTGCTTTTCCTGACTTTCTCAGAGGACAGAGGGGTGAGGCGGGGCAGCTCATGAATAAT
  TTTTTATGTTGAAGACTTGTCGAAATAATATTTTGGTTATGTTGGGTTAAATAAAATATAAAGTTAGTCT
  GTAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC018027 unedited
  GGGGGGNGGGGNNNNNNNNNNNNNCCCNNNNCCGGTTCAGAATTTGTATACGACTCACTA
  TAGGCGGCCGCGNATTCGGCACCAGGCTAGGTCGGCTCGCTGGCCGGGGCTCCGCGGCTC
  CCGTGGTTGCCATGGCGGCGGTTGTCGCGGCGACGAGGTGGTGGCAGCTGTTGCTGGTGC
  TCAGCGCCGCGGGGATGGGGGCCTCGGGCGCCCCGCAGCCCCCCAACATCCTGCTCCTGC
  TCATGGACGACATGAAGGATTTGGGGGCAGAGCACTTGGCAGGTCATGAAGGGGTCCAAC
  TTCTCGGGTTGTTGAACGTCTACCTGGAACAAGAAGAGAGATTCCAACCTCGAGAAAAAG
  GGCTGAGTTTGATTGAGGCTACCCCGGAGAATGATAACACTTTGTGTCCAGGATTGAGAA
  ATGCCAAAGTTGAAGATTTAAGGAGTTTAGCCAACTTTTTTGGATCTTGCACTGAAACTT
  TTGTCCTGGCTGTCAATATTTTGGACAGGTTCTTGGCTCTTATGAAGGTGAAACCTAAAC
  ATTTGTCTTGCATTGGAGTCTGTTCTTTTTTGCTGGCTGCTAGAATAGTTGAAGAAGACT
  GCAATATTCCATCCACTCATGATGTGATCCGGATTAGTCAGTGTAAATGTACTGCTTCTG
  ACATAAAACGGATGGAAAAAATAATTTCAGAAAAATTGCACTATGAATTGGAAGCTACTA
  CTGCCTTNAACTNTNTGCACTTATACCATACTATTATACTNTGTCATACTTCAGAAAGGA
  AGAAATACTGAGCCTTTGATAACTAGAAGCTCAGCTNGAAGCTTTGCACTGCCGACTCAT
  CTTTTCAAAGCAAACCATCTGTATTAGCCTTGTGCCTCTCATTTTTGAAGTGGAAACTTT
  GAAAATCTGTGNAATACTGGNAANTCTCTTGCTAGTTAA
  
• Restriction Sites: NotI-NotI
• ACCN: BC018027
• Insert Size: 2650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018027.1
• RefSeq Size: 737 bp
• RefSeq ORF: 737 bp
• Locus ID: 2588
• Cytogenetics: 16q24.3
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: 'This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq, Jul 2008]'