CD42c (GP1BB) (BC020397) Human Untagged Clone

CAT#: SC106270

(untagged)-Homo sapiens, clone IMAGE:4213319

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GP1BB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC020397, the custom clone sequence may differ by one or more nucleotides
  
  
  GTCTTCCCTTATCCCCAGACGCCTAGCGGGCAGGGTTGGGCTGAATCAAATGGGAGCCCTCCAGACATAA
  GGAGGCCAGAGGCTGCAAGGAGCGGGGTCGTGACCGCTTACACCCCTTCTCCACAGCCCGGCCCGACCTG
  GAGGGCCCCCGGGGCACTGGGCGGTGAGCCACCTCCTGGCAACTCTCGGTGCCGTCCCCTGCCCTCGCTC
  GAGGCCTCTTCTCCCCAGCACCGCTGTGGTGTGCCGGGATCCTGAGCCTAGGCCTCCCGATGTTCCCACC
  CGCATGATCCCTTCCCGCCACACGATGCTCCGTTTTCTTCCGTTGTGAATGCCGCGTCCTGTCCTGGTGA
  CAGGAGAACAATGTTGGTGAACGTCGCAGCGGGTGTCCGAGTGCTCCGTGTGCCCCTGAGAGCGGGTGGG
  AGCGGAAGCCTGAGCGGCCTGCGGCCTCCGGCGATAGTGTGCTATCTGCCGCTGCAGCGCGCGTCCGCGC
  GGCCTCTGGGCTATTTCTGGCCAGGCCGCAGCACTGTGGTCGGTGCGGGCGTGGCAGGGGCGGGGCGGCC
  TTATCGCTCGGCTCTCCCGCCTACGCCTCCCGCTGCAGAGTAAGCCGGGCTGCCGTCTTCTCGCCATGGG
  CTCCGGGCCGCGCGGGGCGCTGAGCTTACTGCTCCTGCTGCTGGCCCCGCCGAGCCGCCCGGCCGCAGGT
  TGCCCGGCGCCCTGTAGCTGCGCGGGGACGCTCGTGGACTGCGGGCGCCGCGGGCTGACTTGGGCCTCGC
  TGCCGACCGCCTTCCCTGTCGACACAACCGAGCTGGTGCTGACCGGCAACAACCTGACGGCGCTGCCGCC
  GGGGCTGCTGGACGCGCTGCCCGCGCTGCGCACCGCACACCTGGGCGCCAACCCCTGGCGCTGCGACTGC
  CGCCTTGTGCCGCTGCGCGCCTGGCTGGCCGGCCGCCCCGAGCGTGCGCCCTACCGCGACCTGCGTTGCG
  TGGCGCCCCCAGCGCTGCGCGGCCGC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC020397 unedited
  GGCCCCCCGGGACCAAACCCCTTTCCCCCCCCCTTACGACAATTGTATACGACTCCTATA
  GGCGGCCGCGNAATTCGCACGAGCTCGGTGCCGTCCCCTGCCCTCGCTCGAGGCCTCTTC
  TCCCCACACCGCTGTGGTGTGCCGGGATCCTGAGCCTAGGCCTCCCGATGTTCCCACCCG
  CATGATCCCTTCCCGCCACACGATGCTCCGTTTTCTTCCGTTGTGAATGCCGCGTCCTGT
  CCTGGTGACAGGAGAACAATGTTGGTGAACGTCGCAGCGGGTGTCCGAGTGCTCCGTGTG
  CCCCTGAGAGCGGGTGGGAGCGGAAGCCTGAGCGGCCTGCGGCCTCCGGCGATAGTGTGC
  TATCTGCCGCTGCAGCGCGCGTCCGCGCGGCCTCTGGGCTATTTCTGGCCAGGCCGCAGC
  ACTGTGGTCGGTGCGGGCGTGGCAGGGGCGGGGCGGCCTTATCGCTCGGCTCTCCCGCCT
  ACGCCTCCCGCTGCAGAGTAAGCCGGGCTGCCGTCTTCTCGCCATGGGCTCCGGGCCGCG
  CGGGGCGCTGAGCTTACTGCTCCTGCTGCTGGCCCCGCCGAGCCGCCCGGCCGCAGGTTG
  CCCGGCGCCCTGTAGCTGCGCGGNGACGCTCGTGGACTGCGGGCGCCGCGGGCTGACTTG
  GGCCTCGCTGCCGACCGCCTTCCCTGTCGACACAACCGAGCTGGTGCTGACCGGCAACAA
  CCTGACGGCGCTGCCGCCGGGGCTGCTGAACCCGCTGCCCCCGCTGCGCACCGCACACCC
  TGGCGCCAACCCCTGGCGCTGCGACTGCCGCCTTGTGCCGCTGCCCCCCTGACTGCCCGG
  CCGCCCCGAGCGTGCGCCCTACCGCGAACTGCGTTGCGTGGCCCCCCCCACGCTGCGCGG
  ACCACTGCTG
  
• Restriction Sites: NotI-NotI
• ACCN: BC020397
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020397.1
• RefSeq Size: 1006 bp
• RefSeq ORF: 1006 bp
• Locus ID: 2812
• Cytogenetics: 22q11.21
• Domains: LRRNT, LRRCT, LRR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ECM-receptor interaction, Hematopoietic cell lineage
• Gene Summary: 'Platelet glycoprotein Ib (GPIb) is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. It is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (VWF), and mediates platelet adhesion in the arterial circulation. GPIb alpha chain provides the VWF binding site, and GPIb beta contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. Mutations in the GPIb beta subunit have been associated with Bernard-Soulier syndrome, velocardiofacial syndrome and giant platelet disorder. The 206 amino acid precursor of GPIb beta is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. A 411 amino acid protein arising from a longer, unspliced transcript in endothelial cells has been described; however, the authenticity of this product has been questioned. Yet another less abundant GPIb beta mRNA species of 3.5 kb, expressed in nonhematopoietic tissues such as endothelium, brain and heart, was shown to result from inefficient usage of a non-consensus polyA signal in the neighboring upstream gene (SEPT5, septin 5). In the absence of polyadenylation from its own imperfect site, the SEPT5 gene produces read-through transcripts that use the consensus polyA signal of this gene. [provided by RefSeq, Dec 2010]'