PTBP1 (NM_031990) Human Untagged Clone

CAT#: SC106769

PTBP1 (untagged)-Human polypyrimidine tract binding protein 1 (PTBP1), transcript variant 2


  "NM_031990" in other vectors (4)

Reconstitution Protocol

USD 930.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PTBP1
Synonyms HNRNP-I; HNRNPI; HNRPI; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC106769 sequence for NM_031990 edited (data generated by NextGen Sequencing)
ATGGACGGCATTGTCCCAGATATAGCCGTTGGTACAAAGCGGGGATCTGACGAGCTTTTC
TCTACTTGTGTCACTAACGGACCGTTTATCATGAGCAGCAACTCGGCTTCTGCAGCAAAC
GGAAATGACAGCAAGAAGTTCAAAGGTGACAGCCGAAGTGCAGGCGTCCCCTCTAGAGTG
ATCCACATCCGGAAGCTCCCCATCGACGTCACGGAGGGGGAAGTCATCTCCCTGGGGCTG
CCCTTTGGGAAGGTCACCAACCTCCTGATGCTGAAGGGGAAAAACCAGGCCTTCATCGAG
ATGAACACGGAGGAGGCTGCCAACACCATGGTGAACTACTACACCTCGGTGACCCCTGTG
CTGCGCGGCCAGCCCATCTACATCCAGTTCTCCAACCACAAGGAGCTGAAGACCGACAGC
TCTCCCAACCAGGCGCGGGCCCAGGCGGCCCTGCAGGCGGTGAACTCGGTCCAGTCGGGG
AACCTGGCCTTGGCTGCCTCGGCGGCGGCCGTGGACGCAGGGATGGCGATGGCCGGGCAG
AGCCCCGTGCTCAGGATCATCGTGGAGAACCTCTTCTACCCTGTGACCCTGGATGTGCTG
CACCAGATTTTCTCCAAGTTCGGCACAGTGTTGAAGATCATCACCTTCACCAAGAACAAC
CAGTTCCAGGCCCTGCTGCAGTATGCGGACCCCGTGAGCGCCCAGCACGCCAAGCTGTCG
CTGGACGGGCAGAACATCTACAACGCCTGCTGCACGCTGCGCATCGACTTTTCCAAGCTC
ACCAGCCTCAACGTCAAGTACAACAATGACAAGAGCCGTGACTACACACGCCCAGACCTG
CCTTCCGGGGACAGCCAGCCCTCGCTGGACCAGACCATGGCCGCGGCCTTCGCCTCTCCG
TATGCAGGAGCTGGTTTCCCTCCCACCTTTGCCATTCCTCAAGCTGCAGGCCTTTCCGTT
CCGAACGTCCACGGCGCCCTGGCCCCCCTGGCCATCCCCTCGGCGGCGGCGGCAGCTGCG
GCGGCAGGTCGGATCGCCATCCCGGGCCTGGCGGGGGCAGGAAATTCTGTATTGCTGGTC
AGCAACCTCAACCCAGAGAGAGTCACACCCCAAAGCCTCTTTATTCTTTTCGGCGTCTAC
GGTGACGTGCAGCGCGTGAAGATCCTGTTCAATAAGAAGGAGAACGCCCTAGTGCAGATG
GCGGACGGCAACCAGGCCCAGCTGGCCATGAGCCACCTGAACGGGCACAAGCTGCACGGG
AAGCCCATCCGCATCACGCTCTCGAAGCACCAGAACGTGCAGCTGCCCCGCGAGGGCCAG
GAGGACCAGGGCCTGACCAAGGACTACGGCAACTCACCCCTGCACCGCTTCAAGAAGCCG
GGCTCCAAGAACTTCCAGAACATATTCCCGCCCTCGGCCACGCTGCACCTCTCCAACATC
CCGCCCTCAGTCTCCGAGGAGGATCTCAAGGTCCTGTTTTCCAGCAATGGGGGCGTCGTC
AAAGGATTCAAGTTCTTCCAGAAGGACCGCAAGATGGCACTGATCCAGATGGGCTCCGTG
GAGGAGGCGGTCCAGGCCCTCATTGACCTGCACAACCACGACCTCGGGGAGAACCACCAC
CTGCGGGTCTCCTTCTCCAAGTCCACCATCTAG

Clone variation with respect to NM_031990.3
>OriGene 5' read for NM_031990 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCACTCCGTCC
CCCGCGGGTCTGCTCTGTGTGCCATGGACGGCATTGTCCCAGATATAGCCGTTGGTACAA
AGCGGGGATCTGACGAGCTTTTCTCTACTTGTGTCACTAACGGACCGTTTATCATGAGCA
GCAACTCGGCTTCTGCAGCAAACGGAAATGACAGCAAGAAGTTCAAAGGTGACAGCCGAA
GTGCAGGCGTCCCCTCTAGAGTGATCCACATCCGGAAGCTCCCCATCGACGTCACGGAGG
GGGAAGTCATCTCCCTGGGGCTGCCCTTTGGGAAGGTCACCAACCTCCTGATGCTGAAGG
GGAAAAACCAGGCCTTCATCGAGATGAACACGGAGGAGGCTGCCAACACCATGGTGAACT
ACTACACCTCGGTGACCCCTGTGCTGCGCGGCCAGCCCATCTACATCCAGTTCTCCAACC
ACAAGGAGCTGAAGACCGACAGCTCTCCCAACCAGGCGCGGGCCCAGGCGGCCCTGCAGG
CGGTGAACTCGGTCCAGTCGGGGAACCTGGCCTTGGCTGCCTCGGCGGCGGCCGTGGACG
CAGGGATGGCGATGGCCGGGCAGAGCCCCGTGCTCAGGATCATCGTGGAGAACCTCTTCT
ACCCTGTGACCCTGGATGTGCTGCACCAAATTNTCTCCAAGTTCGGCACAGTGTTGAAGA
TCATCACCTTCACCAAGAAACACCAGTTCCAGGCCCCTGCTGCAGTATGCGGACCCCGTG
AACCGCCCAACACGCAAGCTGGTCCCTGGACGGGCAGACATCTACAACGCCTGGTGCCCG
CTGGGCATCGACTTTTCAAGCTCACCGGCTCACGTCAAGTACACCATGACAAGAGCGGGA
CTACCACCCCAGACCTG
>OriGene 3' read for NM_031990 unedited
GCCCGTTTCTATATCGAGTTTTTTTTTTTTTTTTTTGGAGTTTCTGAAACGGTTTTAATC
GGTTCTCTCCGCGTCACAAGCCATCGGGTAAGGCAACGGTAATGTGCGTGGGGTCCCCTG
TGGCTTCGTGGTTACAATACTGAGCCTGGAATTGCTGTTAGCAAAATATACATTTGTGTC
ACCATAAAAAACCGCGCCGTCGTCCCTCGGGTCTTACAACAGGTATAAAAAATTATAAAT
ATTTACACCCTTGTTACACGCTTTTACGGAAAGGGGATCCTAAGATAGCCCCCGGGACAG
GACTCGGGGGCGGCACAAAGAGCACAATGACAGGATCGCCCGTCTTTCGGGTTCATCCTC
CTAGGCAGGTTTAACACAGAAATGAGACTTTGGCGCAAAATTTGAAGGAAGAAAAAACCC
TGAAGTTTGAGAGCAAGTTTTTTATGCGACTGCTGGTGAAAGCGTGGTGCAATGCTACCG
GAGGCGATGTCAGAGGCGGTGACACCTTCGCCCCTTACCTGAAGCTTAAGGGCTCTAACA
TAGGACCGGGGCTACCTGTGTCTGACCCATGGGCGGTCCCGTCTGCTCTCTGGAACCTGG
TTAGGACCGGGCTTTCTGTGCCTGCCCACTGGGCGGTCCCGCCTGCTTTCTGGAAACTGG
GGTCTGCCTGTGCCTTATTTCCAGGAATAAATACAGCTACAGCGTCCAGGGCATACCCCT
GGAAATTTTATTTCCCTCCACCCTTGAGTCTCACTGCATAAAAGATACTAAAATATTCCT
TTTGCTTTAATGGATAAGATTCCATATCTGGCTACCGGCAAATTGCTAGGCCACACCTTG
CTCTGCTAGTTTTATTTAGATATTTTTCTTCCCTCCCTACTGAAACCCGGGACCCATTGG
GTGCATAGATAGCCCGGGGCGGGAAAGGAATTCCGTCTGTTAAGGCGGGCGGGGTTCTCC
CC
Restriction Sites NotI-NotI     
ACCN NM_031990
Insert Size 3070 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031990.2, NP_114367.1
RefSeq Size 3300 bp
RefSeq ORF 1653 bp
Locus ID 5725
Cytogenetics 19p13.3
Domains RRM
Protein Families Druggable Genome
Gene Summary 'This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) uses an alternative splice site resulting in it lacking 21 nt in the coding region compared to variant 1. Thus isoform b is 7 aa shorter than isoform a, but it maintains the same reading frame.

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