PTBP1 (NM_031990) Human Untagged Clone

CAT#: SC106769

PTBP1 (untagged)-Human polypyrimidine tract binding protein 1 (PTBP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTBP1
• Synonyms: HNRNP-I; HNRNPI; HNRPI; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106769 sequence for NM_031990 edited (data generated by NextGen Sequencing)
  ATGGACGGCATTGTCCCAGATATAGCCGTTGGTACAAAGCGGGGATCTGACGAGCTTTTC
  TCTACTTGTGTCACTAACGGACCGTTTATCATGAGCAGCAACTCGGCTTCTGCAGCAAAC
  GGAAATGACAGCAAGAAGTTCAAAGGTGACAGCCGAAGTGCAGGCGTCCCCTCTAGAGTG
  ATCCACATCCGGAAGCTCCCCATCGACGTCACGGAGGGGGAAGTCATCTCCCTGGGGCTG
  CCCTTTGGGAAGGTCACCAACCTCCTGATGCTGAAGGGGAAAAACCAGGCCTTCATCGAG
  ATGAACACGGAGGAGGCTGCCAACACCATGGTGAACTACTACACCTCGGTGACCCCTGTG
  CTGCGCGGCCAGCCCATCTACATCCAGTTCTCCAACCACAAGGAGCTGAAGACCGACAGC
  TCTCCCAACCAGGCGCGGGCCCAGGCGGCCCTGCAGGCGGTGAACTCGGTCCAGTCGGGG
  AACCTGGCCTTGGCTGCCTCGGCGGCGGCCGTGGACGCAGGGATGGCGATGGCCGGGCAG
  AGCCCCGTGCTCAGGATCATCGTGGAGAACCTCTTCTACCCTGTGACCCTGGATGTGCTG
  CACCAGATTTTCTCCAAGTTCGGCACAGTGTTGAAGATCATCACCTTCACCAAGAACAAC
  CAGTTCCAGGCCCTGCTGCAGTATGCGGACCCCGTGAGCGCCCAGCACGCCAAGCTGTCG
  CTGGACGGGCAGAACATCTACAACGCCTGCTGCACGCTGCGCATCGACTTTTCCAAGCTC
  ACCAGCCTCAACGTCAAGTACAACAATGACAAGAGCCGTGACTACACACGCCCAGACCTG
  CCTTCCGGGGACAGCCAGCCCTCGCTGGACCAGACCATGGCCGCGGCCTTCGCCTCTCCG
  TATGCAGGAGCTGGTTTCCCTCCCACCTTTGCCATTCCTCAAGCTGCAGGCCTTTCCGTT
  CCGAACGTCCACGGCGCCCTGGCCCCCCTGGCCATCCCCTCGGCGGCGGCGGCAGCTGCG
  GCGGCAGGTCGGATCGCCATCCCGGGCCTGGCGGGGGCAGGAAATTCTGTATTGCTGGTC
  AGCAACCTCAACCCAGAGAGAGTCACACCCCAAAGCCTCTTTATTCTTTTCGGCGTCTAC
  GGTGACGTGCAGCGCGTGAAGATCCTGTTCAATAAGAAGGAGAACGCCCTAGTGCAGATG
  GCGGACGGCAACCAGGCCCAGCTGGCCATGAGCCACCTGAACGGGCACAAGCTGCACGGG
  AAGCCCATCCGCATCACGCTCTCGAAGCACCAGAACGTGCAGCTGCCCCGCGAGGGCCAG
  GAGGACCAGGGCCTGACCAAGGACTACGGCAACTCACCCCTGCACCGCTTCAAGAAGCCG
  GGCTCCAAGAACTTCCAGAACATATTCCCGCCCTCGGCCACGCTGCACCTCTCCAACATC
  CCGCCCTCAGTCTCCGAGGAGGATCTCAAGGTCCTGTTTTCCAGCAATGGGGGCGTCGTC
  AAAGGATTCAAGTTCTTCCAGAAGGACCGCAAGATGGCACTGATCCAGATGGGCTCCGTG
  GAGGAGGCGGTCCAGGCCCTCATTGACCTGCACAACCACGACCTCGGGGAGAACCACCAC
  CTGCGGGTCTCCTTCTCCAAGTCCACCATCTAG
  
  Clone variation with respect to NM_031990.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031990 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCACTCCGTCC
  CCCGCGGGTCTGCTCTGTGTGCCATGGACGGCATTGTCCCAGATATAGCCGTTGGTACAA
  AGCGGGGATCTGACGAGCTTTTCTCTACTTGTGTCACTAACGGACCGTTTATCATGAGCA
  GCAACTCGGCTTCTGCAGCAAACGGAAATGACAGCAAGAAGTTCAAAGGTGACAGCCGAA
  GTGCAGGCGTCCCCTCTAGAGTGATCCACATCCGGAAGCTCCCCATCGACGTCACGGAGG
  GGGAAGTCATCTCCCTGGGGCTGCCCTTTGGGAAGGTCACCAACCTCCTGATGCTGAAGG
  GGAAAAACCAGGCCTTCATCGAGATGAACACGGAGGAGGCTGCCAACACCATGGTGAACT
  ACTACACCTCGGTGACCCCTGTGCTGCGCGGCCAGCCCATCTACATCCAGTTCTCCAACC
  ACAAGGAGCTGAAGACCGACAGCTCTCCCAACCAGGCGCGGGCCCAGGCGGCCCTGCAGG
  CGGTGAACTCGGTCCAGTCGGGGAACCTGGCCTTGGCTGCCTCGGCGGCGGCCGTGGACG
  CAGGGATGGCGATGGCCGGGCAGAGCCCCGTGCTCAGGATCATCGTGGAGAACCTCTTCT
  ACCCTGTGACCCTGGATGTGCTGCACCAAATTNTCTCCAAGTTCGGCACAGTGTTGAAGA
  TCATCACCTTCACCAAGAAACACCAGTTCCAGGCCCCTGCTGCAGTATGCGGACCCCGTG
  AACCGCCCAACACGCAAGCTGGTCCCTGGACGGGCAGACATCTACAACGCCTGGTGCCCG
  CTGGGCATCGACTTTTCAAGCTCACCGGCTCACGTCAAGTACACCATGACAAGAGCGGGA
  CTACCACCCCAGACCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031990 unedited
  GCCCGTTTCTATATCGAGTTTTTTTTTTTTTTTTTTGGAGTTTCTGAAACGGTTTTAATC
  GGTTCTCTCCGCGTCACAAGCCATCGGGTAAGGCAACGGTAATGTGCGTGGGGTCCCCTG
  TGGCTTCGTGGTTACAATACTGAGCCTGGAATTGCTGTTAGCAAAATATACATTTGTGTC
  ACCATAAAAAACCGCGCCGTCGTCCCTCGGGTCTTACAACAGGTATAAAAAATTATAAAT
  ATTTACACCCTTGTTACACGCTTTTACGGAAAGGGGATCCTAAGATAGCCCCCGGGACAG
  GACTCGGGGGCGGCACAAAGAGCACAATGACAGGATCGCCCGTCTTTCGGGTTCATCCTC
  CTAGGCAGGTTTAACACAGAAATGAGACTTTGGCGCAAAATTTGAAGGAAGAAAAAACCC
  TGAAGTTTGAGAGCAAGTTTTTTATGCGACTGCTGGTGAAAGCGTGGTGCAATGCTACCG
  GAGGCGATGTCAGAGGCGGTGACACCTTCGCCCCTTACCTGAAGCTTAAGGGCTCTAACA
  TAGGACCGGGGCTACCTGTGTCTGACCCATGGGCGGTCCCGTCTGCTCTCTGGAACCTGG
  TTAGGACCGGGCTTTCTGTGCCTGCCCACTGGGCGGTCCCGCCTGCTTTCTGGAAACTGG
  GGTCTGCCTGTGCCTTATTTCCAGGAATAAATACAGCTACAGCGTCCAGGGCATACCCCT
  GGAAATTTTATTTCCCTCCACCCTTGAGTCTCACTGCATAAAAGATACTAAAATATTCCT
  TTTGCTTTAATGGATAAGATTCCATATCTGGCTACCGGCAAATTGCTAGGCCACACCTTG
  CTCTGCTAGTTTTATTTAGATATTTTTCTTCCCTCCCTACTGAAACCCGGGACCCATTGG
  GTGCATAGATAGCCCGGGGCGGGAAAGGAATTCCGTCTGTTAAGGCGGGCGGGGTTCTCC
  CC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031990
• Insert Size: 3070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031990.2, NP_114367.1
• RefSeq Size: 3300 bp
• RefSeq ORF: 1653 bp
• Locus ID: 5725
• Cytogenetics: 19p13.3
• Domains: RRM
• Protein Families: Druggable Genome
• Gene Summary: 'This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternative splice site resulting in it lacking 21 nt in the coding region compared to variant 1. Thus isoform b is 7 aa shorter than isoform a, but it maintains the same reading frame.