EVI1 (MECOM) (NM_005241) Human Untagged Clone

CAT#: SC107040

MECOM (untagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MECOM
• Synonyms: AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1; PRDM3; RUSAT2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107040 sequence for NM_005241 edited (data generated by NextGen Sequencing)
  ATGAAGAGCGAAGACTATCCCCATGAAACTATGGCGCCGGATATCCACGAAGAACGGCAA
  TATCGCTGCGAAGACTGTGACCAGCTCTTTGAATCTAAGGCTGAACTAGCAGATCACCAA
  AAGTTTCCATGCAGTACTCCTCACTCAGCATTTTCAATGGTTGAAGAGGACTTTCAGCAA
  AAACTCGAAAGCGAGAATGATCTCCAAGAGATACACACGATCCAGGAGTGTAAGGAATGT
  GACCAAGTTTTTCCTGATTTGCAAAGCCTGGAGAAACACATGCTGTCACATACTGAAGAG
  AGGGAATACAAGTGTGATCAGTGTCCCAAGGCATTTAACTGGAAGTCCAATTTAATTCGC
  CACCAGATGTCACATGACAGTGGAAAGCACTATGAATGTGAAAACTGTGCCAAGGTTTTC
  ACGGACCCTAGCAACCTTCAGCGGCACATTCGCTCTCAGCATGTCGGTGCCCGGGCCCAT
  GCATGCCCGGAGTGTGGCAAAACGTTTGCCACTTCGTCGGGCCTCAAACAACACAAGCAC
  ATCCACAGCAGTGTGAAGCCCTTTATCTGTGAGGTCTGCCATAAATCCTATACTCAGTTT
  TCAAACCTTTGCCGTCATAAGCGCATGCATGCTGATTGCAGAACCCAAATCAAGTGCAAA
  GACTGTGGACAAATGTTCAGCACTACGTCTTCCTTAAATAAACACAGGAGGTTTTGTGAG
  GGCAAGAACCATTTTGCGGCAGGTGGATTTTTTGGCCAAGGCATTTCACTTCCTGGAACC
  CCAGCTATGGATAAAACGTCCATGGTTAATATGAGTCATGCCAACCCGGGCCTTGCTGAC
  TATTTTGGCGCCAATAGGCATCCTGCTGGTCTTACCTTTCCAACAGCTCCTGGATTTTCT
  TTTAGCTTCCCTGGTCTGTTTCCTTCCGGCTTGTACCACAGGCCTCCTTTGATACCTGCT
  AGTTCTCCTGTTAAAGGACTATCAAGTACTGAACAGACAAACAAAAGTCAAAGTCCCCTC
  ATGACACATCCTCAGATACTGCCAGCTACACAGGATATTTTGAAGGCACTATCTAAACAC
  CCATCTGTAGGGGACAATAAGCCAGTGGAGCTCCAGCCCGAGAGGTCCTCTGAAGAGAGG
  CCCTTTGAGAAAATCAGTGACCAGTCAGAGAGTAGTGACCTTGATGATGTCAGTACACCA
  AGTGGCAGTGACCTGGAAACAACCTCGGGCTCTGATCTGGAAAGTGACATTGAAAGTGAT
  AAAGAGAAATTTAAAGAAAATGGTAAAATGTTCAAAGACAAAGTAAGCCCTCTTCAGAAT
  CTGGCTTCAATAAATAATAAGAAAGAATACAGCAATCATTCCATTTTCTCACCATCTTTA
  GAGGAGCAGACTGCGGTGTCAGGAGCTGTGAATGATTCTATAAAGGCTATTGCTTCTATT
  GCTGAAAAATACTTTGGTTCAACAGGACTGGTGGGGCTGCAAGACAAAAAAGTTGGAGCT
  TTACCTTACCCTTCCATGTTTCCCCTCCCATTTTTTCCAGCATTCTCTCAATCAATGTAC
  CCATTTCCTGATAGAGACTTGAGATCGTTACCTTTGAAAATGGAACCCCAATCACCAGGT
  GAAGTAAAGAAACTGCAGAAGGGCAGCTCTGAGTCCCCCTTTGATCTCACCACTAAGCGA
  AAGGATGAGAAGCCCTTGACTCCAGTCCCCTCCAAGCCTCCAGTGACACCTGCCACAAGC
  CAAGACCAGCCCCTGGATCTAAGTATGGGCAGTAGGAGTAGAGCCAGTGGGACAAAGCTG
  ACTGAGCCTCGAAAAAACCACGTGTTTGGGGGAAAAAAAGGAAGCAACGTCGAATCAAGA
  CCTGCTTCAGATGGTTCCTTGCAGCATGCAAGACCCACTCCTTTCTTTATGGACCCTATT
  TACAGAGTAGAGAAAAGAAAACTAACTGACCCACTTGAAGCTTTAAAAGAGAAATACTTG
  AGGCCTTCTCCAGGATTCTTGTTTCACCCACAATTCCAACTGCCTGATCAGAGAACTTGG
  ATGTCAGCTATTGAAAACATGGCAGAAAAGCTAGAGAGCTTCAGTGCCCTGAAACCTGAG
  GCCAGTGAGCTCTTACAGTCAGTGCCCTCTATGTTCAACTTCAGGGCGCCTCCCAATGCC
  CTGCCAGAGAACCTTCTGCGGAAGGGAAAGGAGCGCTATACCTGCAGATACTGTGGCAAG
  ATTTTTCCAAGGTCTGCAAACCTAACACGGCACTTGAGAACCCACACAGGAGAGCAGCCT
  TACAGATGCAAATACTGTGACAGATCATTTAGCATATCTTCTAACTTGCAAAGGCATGTT
  CGCAACATCCACAATAAAGAGAAGCCATTTAAGTGTCACTTATGTGATAGGTGTTTTGGT
  CAACAAACCAATTTAGACAGACACCTAAAGAAACATGAGAATGGGAACATGTCCGGTACA
  GCAACATCGTCGCCTCATTCTGAACTGGAAAGTACAGGTGCGATTCTGGATGACAAAGAA
  GATGCTTACTTCACAGAAATTCGAAATTTCATTGGGAACAGCAACCATGGCAGCCAATCT
  CCCAGGAATGTGGAGGAGAGAATGAATGGCAGTCATTTTAAAGATGAAAAGGCTTTGGTG
  ACCAGTCAAAATTCAGACTTGCTGGATGATGAAGAAGTTGAAGATGAGGTGTTGTTAGAT
  GAGGAGGATGAAGACAATGATATTACTGGAAAAACAGGAAAGGAACCAGTGACAAGTAAT
  TTACATGAAGGAAACCCTGAGGATGACTATGAAGAAACCAGTGCCCTGGAGATGAGTTGC
  AAGACATCCCCAGTGAGGTATAAAGAGGAAGAATATAAAAGTGGACTTTCTGCTCTAGAT
  CATATAAGGCACTTCACAGATAGCCTCAAAATGAGGAAAATGGAAGATAATCAATATTCT
  GAAGCTGAGCTGTCTTCTTTTAGTACTTCCCATGTGCCAGAGGAACTTAAGCAGCCGTTA
  CACAGAAAGTCCAAATCGCAGGCATATGCTATGATGCTGTCACTGTCTGACAAGGAGTCC
  CTCCATTCTACATCCCACAGTTCTTCCAACGTGTGGCACAGTATGGCCAGGGCTGCGGCG
  GAATCCAGTGCTATCCAGTCCATAAGCCACGTATGA
  
  Clone variation with respect to NM_005241.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005241 unedited
  ACAGTAATTACCCCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA
  TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC
  GGCCGCGAATTCGGCACGAGGCCGCAGTCTCGCGGAGCCCTGCTGCTTATCTACGTTGCT
  AAGCCGGGCGATTTCCTTGTTCCTCCTGCGAAACGGTGCGGTCTGGACACGTCTCCGGGG
  TGGGTCGTCCGGCCTTCGATCTTAGACGAATTTTACAATGTGAAGTTCTGCATAGATGCC
  AGTCAACCAGATGTTGGAAGCTGGCTCAAGTACATTAGATTCGCTGGCTGTTATGATCAG
  CACAACCTTGTTGCATGCCAGATAAATGATCAGATATTCTATAGAGTAGTTGCAGACATT
  GCGCCGGGAGAGGAGCTTCTGCTGTTCATGAAGAGCGAAGACTATCCCCATGAAACTATG
  GCGCCGGATATCCACGAAGAACGGCAATATCGCTGCGAAGACTGTGACCAGCTCTTTGAA
  TCTAAGGCTGAACTAGCAGATCACCAAAAGTTTCCATGCAGTACTCCTCACTCAGCATTT
  TCAATGGTTGAAGAGGACTTTCAGCAAAAACTCGAAAGCGAGAATGATCTCCAAGAGATA
  CACACGATCCAGGAGTGTAAGGAATGTGACCAAGTTTTTCCTGATTTGCAAAGCCTGGAG
  AACACATGCTGTCACATACTGAAGAGAGGGAATACACGTGTGATCAGTGTCCCAAGGCAT
  TTAACTGGCAGTCCAATTTAATTCGCCACCAGATGTCACATGACAGTGGAAAGCACTATG
  AATGTGACCACTGTGCCCAGGTTTTACGGACCCTAGCACCTTCAGGGGACATTCGCTCTC
  GCATGTCGGTGCCCGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005241 unedited
  TCGATCATCGGCTGATGGCAACTTCCCAGTTCCAGGANAGCACTGGGGCAGGGAGTCACA
  GGGCATGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAG
  TCGAGTTTTTTTTTTTTTTTTTTTCTTTTTAAAATCATTCAGTTTAAGGTCACTAGACTT
  TAGATGAGTGACCCTGCAGGTTTATAAGGCATTCTGCTCAGCAGTCTTGTAAATAGTCCT
  ATATGAAAGAGCCATGCTACTGTTGGACTTGGTCCCACTCTGGTCAACCTTGATAACGTC
  ATACGTGGCTTATGGACTGGATAGCACTGGATTCCGCCGCAGCCCTGGCCATACTGTGCC
  ACACGTTGGAAGAACTGTGGGATGTAGAATGGAGGGACTCCTTGTCAGACAGTGACAGCA
  TCATAGCATATGCCTGCGATTTGGACTTTCTGTGTAACGGCTGCTTAAGTTCCTCTGGCA
  CATGGGAAGTACTAAAAGAAGACAGCTCAGCTTCAGAATATTGATTATCTTCCATTTTCC
  TCATTTTGAGGCTATCTGTGAAGTGCCTTATATGATCTAGAGCAGAAAGTCCACTTTTAT
  ATTCTTCCTCTTTATACCTCACTGGGGATGTCTTGCAACTCATCTCCAGGGCACTGGTTT
  CTTCATAGTCATCCTCAGGGTTTCCTTCATGTAAATTACTTGTCACTGGTTCCTTTCCTG
  TTTTTCCAGTAATATCATTGTCTTCATCCTCCTCATCTAACAACACCTCATCTTCAACTT
  CTTTATCATCCAGCAAGTCTGAATTTTGACTGGTCACCAAGCCTTTTCATCTTTAAATGA
  CTGCCTTTCTTCTCTTCTCCACATTCCTGGGAGATTGGCTGCCATGGTTGCTGTTCCCAT
  GAAATTTCAAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005241
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005241.1, NP_005232.1
• RefSeq Size: 3570 bp
• RefSeq ORF: 3156 bp
• Locus ID: 2122
• Cytogenetics: 3q26.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chronic myeloid leukemia, MAPK signaling pathway, Pathways in cancer
• Gene Summary: 'The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (2, also known as EVI1_1c) lacks a portion of the 5' UTR and 5' coding region, initiates translation at a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 7 all encode the same isoform.