SH3MD2 (SH3RF1) (NM_020870) Human Untagged Clone

CAT#: SC107385

SH3RF1 (untagged)-Human SH3 domain containing ring finger 1 (SH3RF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SH3RF1
• Synonyms: POSH; RNF142; SH3MD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107385 sequence for NM_020870 edited (data generated by NextGen Sequencing)
  ATGGATGAATCAGCCTTGTTGGATCTTTTGGAGTGTCCGGTGTGTCTAGAGCGCCTTGAT
  GCTTCTGCGAAGGTCTTGCCTTGCCAGCATACGTTTTGCAAGCGATGTTTGCTGGGGATC
  GTAGGTTCTCGAAATGAACTCAGATGTCCCGAGTGCAGGACTCTTGTTGGCTCGGGTGTC
  GAGGAGCTTCCCAGTAACATCTTGCTGGTCAGACTTCTGGATGGCATCAAACAGAGGCCT
  TGGAAACCTGGTCCTGGTGGGGGAAGTGGGACCAACTGCACAAATGCATTAAGGTCTCAG
  AGCAGCACTGTGGCTAATTGTAGCTCAAAAGATCTGCAGAGCTCCCAGGGCGGACAGCAG
  CCTCGGGTGCAATCCTGGAGCCCCCCAGTGAGGGGTATACCTCAGTTACCATGTGCCAAA
  GCATTATACAACTATGAAGGAAAAGAGCCTGGAGACCTTAAATTCAGCAAAGGTGACATC
  ATCATTTTGCGAAGACAAGTGGATGAAAATTGGTACCATGGGGAAGTCAATGGAATCCAT
  GGCTTTTTCCCCACCAACTTTGTGCAGATTATTAAACCGTTACCTCAGCCCCCACCTCAG
  TGCAAAGCACTTTATGACTTTGAAGTGAAAGACAAGGAAGCAGACAAAGATTGCCTTCCA
  TTTGCAAAGGATGATGTTCTGACTGTGATCCGAAGAGTGGATGAAAACTGGGCTGAAGGA
  ATGCTGGCAGACAAAATAGGAATATTTCCAATTTCATATGTTGAGTTTAACTCGGCTGCT
  AAGCAGCTGATAGAATGGGATAAGCCTCCTGTGCCAGGAGTTGATGCTGGAGAATGTTCC
  TCGGCAGCAGCCCAGAGCAGCACTGCCCCAAAGCACTCCGACACCAAGAAGAACACCAAA
  AAGCGGCACTCCTTCACTTCCCTCACTATGGCCAACAAGTCCTCCCAGGCATCCCAGAAC
  CGCCACTCCATGGAGATCAGCCCCCCTGTCCTCATCAGCTCCAGCAACCCCACTGCTGCT
  GCACGGATCAGCGAGCTGTCTGGGCTCTCCTGCAGTGCCCCTTCTCAGGTTCATATAAGT
  ACCACCGGGTTAATTGTGACCCCGCCCCCAAGCAGCCCAGTGACAACTGGCCCCTCGTTT
  ACTTTCCCATCAGATGTTCCCTACCAAGCTGCCCTTGGAACTTTGAATCCTCCTCTTCCA
  CCACCCCCTCTCCTGGCTGCCACTGTCCTTGCCTCCACACCACCAGGCGCCACCGCCGCT
  GCTGCTGCTGCTGGAATGGGACCGAGGCCCATGGCAGGATCCACTGACCAGATTGCACAT
  TTACGGCCGCAGACTCGCCCCAGTGTGTATGTTGCTATATATCCATACACTCCTCGGAAA
  GAGGATGAACTAGAGCTGAGAAAAGGGGAGATGTTTTTAGTGTTTGAGCGCTGCCAGGAT
  GGCTGGTTCAAAGGGACATCCATGCATACCAGCAAGATAGGGGTTTTCCCTGGCAATTAT
  GTGGCACCAGTCACAAGGGCGGTGACAAATGCTTCCCAAGCTAAAGTCCCTATGTCTACA
  GCTGGCCAGACAAGTCGGGGAGTGACCATGGTCAGTCCTTCCACGGCAGGAGGGCCTGCC
  CAGAAGCTCCAGGGAAATGGCGTGGCTGGGAGTCCCAGTGTTGTCCCCGCAGCTGTGGTA
  TCAGCAGCTCACATCCAGACAAGTCCTCAGGCTAAGGTCTTGTTGCACATGACGGGGCAA
  ATGACAGTCAACCAGGCCCGCAATGCTGTGAGGACAGNNNNAGCGCACAACCAGGAACGC
  CCCACGGCAGCAGTGACACCCATCCAGGTACAGAATGCCGCCGGCCTCAGCCCTGCATCT
  GTGGGCCTGTCCCATCACTCGCTGGCCTCCCCACAACCTGCGCCTCTGATGCCAGGCTCA
  GCCACGCACACTGCTGCCATCAGTATCAGTCGAGCCAGTGCCCCTCTGGCCTGTGCAGCA
  GCTGCTCCACTGACTTCCCCAAGCATCACCAGTGCTTCTCTGGAGGCTGAGCCCAGTGGC
  CGGATAGTGACCGTTCTCCCTGGACTCCCCACATCTCCTGACAGTGCTTCATCAGCTTGT
  GGGAACAGTTCAGCAACCAAACCAGACAAGGATAGCAAAAAAGAAAAAAAGGGTTTGTTG
  AAGTTGCTTTCTGGCGCCTCCACTAAACGGAAGCCCCGCGTGTCTCCTCCAGCATCGCCC
  ACCCTAGAAGTGGAGCTGGGCAGTGCAGAGCTTCCTCTCCAGGGAGCGGTGGGGCCCGAA
  CTGCCACCAGGAGGTGGCCATGGCAGGGCAGGCTCCTGCCCTGTGGACGGGGACGGACCG
  GTCACGACTGCAGTGGCAGGAGCAGCCCTGGCCCAGGATGCTTTTCATAGGAAGGCAAGT
  TCCCTGGACTCCGCAGTTCCCATCGCTCCACCTCCTCGCCAGGCCTGTTCCTCCCTGGGT
  CCTGTCTTGAATGAGTCTAGACCTGTCGTTTGTGAAAGGCACAGGGTGGTGGTTTCCTAT
  CCTCCTCAGAGTGAGGCAGAACTTGAACTTAAAGAAGGAGATATTGTGTTTGTTCATAAA
  AAACGAGAGGATGGCTGGTTCAAAGGCACATTACAACGTAATGGGAAAACTGGCCTTTTC
  CCAGGAAGCTTTGTGGAAAACATATGA
  
  Clone variation with respect to NM_020870.3
  1778 t=>n;1779 t=>n;1780 g=>n;1781 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020870 unedited
  GCGCGCGATTCCGGAGCGAGCGGGGGGGGCGCATCTGCGTCANCCGCCGCGGGCGCGGGG
  GGGCCGCGAACAAAGAGGAGGAGCCGAGGCGCGAGAGCAAAGTCTGAGATGGATGTGACA
  TGAGTCATTTTAAGGGGTGCGCACAACTATGAACATTTCTGAAGATTTTTTCTCAGTAAA
  GTAAATAAAGATGGGTGAATCANGCTTGGTGGGTCTTTTGGAGGGGCCGGGGGCGCTANA
  NGGCGCTTGGTGCGTTCTGCGAAGGGGTTGCGCTTGGCANCATACGTTTTCGAGGCATGT
  GTGGTGGGGATCGTANGGTCTCNAAATGAACTCANATGGCCCGAGGGCAGGACTCTTGGT
  GGCTCNGGTGGCNAGGGGCTTCCCANTAACATCTTGGTGGGCANACTTCTGGATGGGATC
  AAACAGGGGCCTTGGAAACCTGGTCCTGGTGGGGGAAGGNGGACCAACTGCGCAAATGCA
  TTAAGGGCTCANAGCAGGACTGGGGGTAATTGGANCTCAAAAATCTGCAAAGCTCCAGGG
  GGGACAGCAGGCTCNGGTGGCGATCTGGAGCCCCCCGTGGGGGGATACCTCAGGTACCAT
  GGCCAAAGCATATACAACTTGAAGGAAAGAGCCTGGAGACCTAATTGAGGGAGGGGAGCA
  TATCATTTTGCGAGACAGGGGGTGAAATCTGGCCCTGGGGGAGCAAGGGGATCCTGGGTT
  TTCCCACAACTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020870 unedited
  CCGTAAGGCCTTGGCTGATGGCACCTTCCAGGTCCAGGATAGCACTGGGGAAGGGTCACA
  GGGCATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTC
  GAGTTTTTTTTTTTTTTTTTTTTTTTTTTCTTGAACATCAAATTTTAATCTTGAAACCTT
  TTATCCAGTCCTCAAATTATTAACATGAAAAGGAGTGATAAATTGCAATTTTATCATTAC
  CATATCACTGTGTAACAAGCCCTTGTTACAAAATCTCCCTCTACTGTCTGCAAAAAACCA
  ATAGAAAACCCATACCTTATATTACCTAATGATCTATTAACAGATGAAATTTTAACCAAC
  TTTATACCAGGAAACTATGAACAGAGGTACTTCAAATCACATAGCTTAAAATATGGAGAA
  AAGACAGGTAAAAAAATTATCTTAACCTGTAGTAGTCTTTTTTCTTTTTAAAATTTTTAT
  AAAATACACTAATTTCCCAAAATAAAGAATATTATGACACATTGGTGTCAACTTACACAG
  ATGAAAGCTGATGCCAGCTTTTTCCTAACTAAAGTTCATTTCACCAAACCTTTATATATT
  ACATGAATATCCAAGTAAAGTATTTTTTTTTTTAAATTCAACACACAGGAACATAATATA
  CCTTGTTTTTTTTTTAAAAAAAGGTTAATTTAAGAATGATTTCATCTTCAACCACATAAA
  GTATCTTTTTAAGACCATGAAACCTCTTGTCATTGCAAGACTTTTCAGTTCATGTTTCTA
  TTTCCCAGTTTTAATTCTTAAAGGGAAAAAAAAAAGTGAAAGGGTTTATTATGTTTTTCC
  ATGAAAAAACTGTGGTAAAGATCTTATTTCATTACGGGCTGTCTTCCCTCCCTTTGTTTT
  CCGGATACCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020870
• ORF Size: 2667 bp
• Insert Size: 6000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_020870.2, NP_065921.2
• RefSeq Size: 5196
• RefSeq ORF: 2667
• Locus ID: 57630
• Gene Summary: This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. [provided by RefSeq, Jul 2008]