Ephrin A1 (EFNA1) (NM_182685) Human Untagged Clone

CAT#: SC107521

EFNA1 (untagged)-Human ephrin-A1 (EFNA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EFNA1
• Synonyms: B61; ECKLG; EFL1; EPLG1; LERK-1; LERK1; TNFAIP4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107521 sequence for NM_182685 edited (data generated by NextGen Sequencing)
  ATGGAGTTCCTCTGGGCCCCTCTCTTGGGTCTGTGCTGCAGTCTGGCCGCTGCTGATCGC
  CACACCGTCTTCTGGAACAGTTCAAATCCCAAGTTCCGGAATGAGGACTACACCATACAT
  GTGCAGCTGAATGACTACGTGGACATCATCTGTCCGCACTATGAAGATCACTCTGTGGCA
  GACGCTGCCATGGAGCAGTACATACTGTACCTGGTGGAGCATGAGGAGTACCAGCTGTGC
  CAGCCCCAGTCCAAGGACCAAGTCCGCTGGCAGTGCAACCGGCCCAGTGCCAAGCATGGC
  CCGGAGAAGCTGTCTGAGAAGTTCCAGCGCTTCACACCTTTCACCCTGGGCAAGGAGTTC
  AAAGAAGGACACAGCTACTACTACATCTCTCACAGTCCTCAGGCCCATGTCAATCCACAG
  GAGAAGAGACTTGCAGCAGATGACCCAGAGGTGCGGGTTCTACATAGCATCGGTCACAGT
  GCTGCCCCACGCCTCTTCCCACTTGCCTGGACTGTGCTGCTCCTTCCACTTCTGCTGCTG
  CAAACCCCGTGA
  
  Clone variation with respect to NM_182685.1
  410 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182685 unedited
  NGGTGCAAATATTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCAGA
  CCCATAGGAGACCCGCGTCCCCGCTCGGCCTGGCCAGGCCCCGCGCTATGGAGTTCCTCT
  GGGCCCCTCTCTTGGGTCTGTGCTGCAGTCTGGCCGCTGCTGATCGCCACACCGTCTTCT
  GGAACAGTTCAAATCCCAAGTTCCGGAATGAGGACTACACCATACATGTGCAGCTGAATG
  ACTACGTGGACATCATCTGTCCGCACTATGAAGATCACTCTGTGGCAGACGCTGCCATGG
  AGCAGTACATACTGTACCTGGTGGAGCATGAGGAGTACCAGCTGTGCCAGCCCCAGTCCA
  AGGACCAAGTCCGCTGGCAGTGCAACCGGCCCAGTGCCAAGCATGGCCCGGAGAAGCTGT
  CTGAGAAGTTCCAGCGCTTCACACCTTTCACCCTGNGCAAGGAGTTCAAAGAAGGACACA
  GCTACTACTACATCTCTCACAGTCCTCAGGCCCATGTCAATCCACAGGAGAAGAGACTTG
  CAGCAGATGACCCAGAGGTGCGGGTTCTACATAGCATCGGTCACAGTGCTGCCCCACGCC
  TCTTCCCACTTGCCTGGACTGTGCTGCTCCTTCCACTTCTGCTGCTGCAAACCCCGTGAA
  GGTGTATGCCACACCTGGCCTTANAGAGGGACAGGCTGAAGAGAGGGACAGGCACTCCAN
  ACCTGTCTTGGGGCCACTNTCAGAGCCCCCAGCCCCTGGAACCACTCCCACCACAGGCAT
  AAGCTATCACCTAGCAGCCTCAAACGGGTCAGTATTAAAGTTTTTCACCGGAAGGGAGCC
  AACCAGCCCCGAAGTGCCATCCCCACCTTCACCTCGNAGGGATGGAGAAAGAGTGGAGAC
  AGTCCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_182685 unedited
  GCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCCGAAAAAACACATTGCTTT
  ATTTTGAGACAAAGAACATTGTATAAAAAAACTCCAGTTGTAGAAATCAGGACAGACACA
  CAGCAACTTATATACAGATACTATACACGTGGGCCCTGCCCTTTAGGCTACACTCTAGGC
  AGAATGTACACATGGGCACTGCCCTTACAGCTACACTCTAAGAACAGGTTGGCACAGCCC
  CCCTGCTGGCACAGCTCTGGGGAGTGCTGCCCCAGGATGGGAGAGAATGCAGTACCTGGC
  TACAAACTTCTCTGTGGCAGCTCCACAGATGAGGTCTTGCTGGGAGATGCTGGGTCAGCC
  CAAGCATGCTGCCTCTCCTGTACCTGGCTTCCAAGCAAGAAACTGTTTCTTGCTTTTCCT
  TCAGTCAGTTCATCTGGGCATCCTGGCTCTCTCCTGGAGGGGGCATCTTTGACAAGCTTT
  GCCCATCCACGTGGCCCCTTCCAGCTCAGCTCCCACTGTGCCTTAAGGGACCATGCCTGC
  ACAGCTTGTTTCTTTGGCTTAAAGGCAGGAATGGTGGGAAAGGACTGTCTCCACTTCTTT
  CTCCATCCCTCCGAGGTGAAGGTGGNGATGGCACTGTCGGGCTGGTTGGCCTCCTTCCGG
  TTGAAAACCTTAATACTGACCCGTTNTGAGGCTGCTAGGTGATAGCTTATGCCTGTGGGG
  GGAGTGGTCCCAGGGCTGGGGCTCTGAAAGTGCCCCAGACAAGTTTGGAGTGCCTGTCCC
  TCTCTCAGCCTGTCCCTCTTAGGCCAGGGGTGGGATACACCTTCACGGGGNTTGCAGCAC
  CAAAAGTGGGAAGGGCACCACAGTCCANGCAAGGGGAAAAAGCGTGGGCAACCCTGGGAC
  CGATGCTATGAAAAACCGCACCTTTGGTCATCTGTGCAAGCCCTCCTTCTGGGGATGGCT
  GA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182685
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182685.1, NP_872626.1
• RefSeq Size: 1524 bp
• RefSeq ORF: 552 bp
• Locus ID: 1942
• Cytogenetics: 1q22
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Gene Summary: 'This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1. The resulting protein (isoform b) is shorter when compared to isoform a.