EIF5 (NM_183004) Human Untagged Clone

CAT#: SC107610

EIF5 (untagged)-Human eukaryotic translation initiation factor 5 (EIF5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF5
• Synonyms: EIF-5; EIF-5A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_183004, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGTCAATGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATGCCCCGTCTGATTGCCA
  AGGTTGAGGGCAAAGGCAATGGAATCAAGACAGTTATAGTCAACATGGTTGACGTTGCAAAGGCGCTTAA
  TCGGCCTCCAACGTATCCCACCAAATATTTTGGTTGTGAGCTGGGAGCACAGACCCAGTTTGATGTTAAG
  AATGACCGTTACATTGTCAATGGATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTCATTA
  AAAAATTTGTTCTCTGTCCTGAATGTGAGAATCCTGAAACAGATTTGCATGTCAATCCAAAGAAGCAAAC
  AATAGGTAATTCTTGTAAAGCCTGTGGCTATCGAGGCATGCTTGACACACATCATAAACTCTGCACATTC
  ATTCTCAAAAACCCACCTGAGAATAGTGACAGTGGTACAGGAAAGAAAGAAAAAGAAAAGAAAAACAGAA
  AGGGCAAAGACAAGGAAAATGGCTCCGTATCCAGCAGTGAGACACCACCACCACCACCACCACCAAATGA
  AATTAATCCTCCTCCACATACAATGGAAGAAGAGGAGGATGATGACTGGGGAGAAGATACAACTGAGGAA
  GCTCAAAGGCGTCGAATGGATGAAATCAGTGACCATGCAAAAGTTCTGACACTCAGTGATGATTTGGAAA
  GAACAATTGAGGAGAGGGTCAATATCCTCTTTGATTTTGTTAAGAAAAAGAAAGAAGAGGGTGTTATTGA
  TTCATCTGACAAAGAAATCGTTGCTGAAGCAGAAAGACTGGATGTAAAAGCCATGGGCCCTCTTGTTCTA
  ACTGAAGTTCTTTTTAATGAGAAGATTAGAGAACAGATTAAGAAATACAGGCGCCATTTCCTACGATTTT
  GTCACAACAACAAAAAAGCCCAACGGTACCTTCTTCATGGTTTGGAGTGTGTGGTAGCAATGCATCAAGC
  TCAGCTTATCTCCAAGATTCCACATATCTTGAAGGAGATGTACGATGCAGACCTTTTAGAAGAAGAGGTC
  ATCATCAGCTGGTCGGAAAAGGCCTCTAAGAAATATGTCTCCAAAGAACTTGCCAAAGAGATTCGTGTCA
  AAGCAGAACCATTTATAAAATGGTTGAAGGAGGCAGAGGAAGAATCTTCTGGTGGCGAAGAAGAAGATGA
  AGATGAGAACATTGAGGTGGTGTATTCGAAGGCTGCCAGTGTACCGAAAGTTGAGACTGTAAAGTCAGAC
  AACAAGGATGACGACATCGATATTGATGCCATTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_183004 unedited
  GGGGGCACTGTATTCGGCACCAGCTGGGGCCGAGGGTAGCTTGAGCGCGGCGGCGGCGTT
  GTTCAGTCAGAGCGAGAACATTCCAGAGGTCGCCCGGCCGCTCCTCGCTGCGCTTCGCCT
  CCGCCTCCTCGGACTCGGACTCGGGTTTATATCGCGCCTCACTTCATCCCAGTCCCGGGC
  GAGCAGCGTTGGGTTTATGGCTCCATTTGACGAAAACGAGCTGTTGCGCAGCCATTGGTA
  CCTGTATTGGGGAAACATAGCATACAAGCAAGAAGCTTACAGCCTCAGTGGCGAAAATTT
  TTTCATGTCAGAGACCGAGAACTCTTGCAGTCGTTTATGTCATCCCTTCTTCTCCAGACA
  GAAGATACCAAAAAGTTGCAATCAAAGATCTCTTCATCTTATTGATAAAGCCACTAATAA
  GCCAAAATGTCTGTCAATGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATG
  CCCCGTCTGATTGCCAAGGTTGAGGGCAAAGGCAATGGAATCAAGACAGTTATAGTCAAC
  ATGGTTGACGTTGCAAAGGCGCTTAATCGGCCTCCAACGTATCCCACCAAATATTTTGGT
  TGTGAGCTGGGAGCACAGACCCAGTTTTGATGTTAAGAATGACCGTTACATTGTCAATGG
  ATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_183004
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183004.3, NP_892116.2
• RefSeq Size: 5782 bp
• RefSeq ORF: 1296 bp
• Locus ID: 1983
• Cytogenetics: 14q32.32
• Gene Summary: 'Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.