Gelsolin (GSN) (NM_198252) Human Untagged Clone

CAT#: SC107769

GSN (untagged)-Human gelsolin (GSN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSN
• Synonyms: ADF; AGEL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_198252, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGGTGGAACACCCCGAGTTCCTCAAGGCAGGGAAGGAGCCTGGCCTGCAGATCTGGCGTGTGGAGA
  AGTTCGATCTGGTGCCCGTGCCCACCAACCTTTATGGAGACTTCTTCACGGGCGACGCCTACGTCATCCT
  GAAGACAGTGCAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACTACTGGCTGGGCAATGAGTGCAGC
  CAGGATGAGAGCGGGGCGGCCGCCATCTTTACCGTGCAGCTGGATGACTACCTGAACGGCCGGGCCGTGC
  AGCACCGTGAGGTCCAGGGCTTCGAGTCGGCCACCTTCCTAGGCTACTTCAAGTCTGGCCTGAAGTACAA
  GAAAGGAGGTGTGGCATCAGGATTCAAGCACGTGGTACCCAACGAGGTGGTGGTGCAGAGACTCTTCCAG
  GTCAAAGGGCGGCGTGTGGTCCGTGCCACCGAGGTACCTGTGTCCTGGGAGAGCTTCAACAATGGCGACT
  GCTTCATCCTGGACCTGGGCAACAACATCCACCAGTGGTGTGGTTCCAACAGCAATCGGTATGAAAGACT
  GAAGGCCACACAGGTGTCCAAGGGCATCCGGGACAACGAGCGGAGTGGCCGGGCCCGAGTGCACGTGTCT
  GAGGAGGGCACTGAGCCCGAGGCGATGCTCCAGGTGCTGGGCCCCAAGCCGGCTCTGCCTGCAGGTACCG
  AGGACACCGCCAAGGAGGATGCGGCCAACCGCAAGCTGGCCAAGCTCTACAAGGTCTCCAATGGTGCAGG
  GACCATGTCCGTCTCCCTCGTGGCTGATGAGAACCCCTTCGCCCAGGGGGCCCTGAAGTCAGAGGACTGC
  TTCATCCTGGACCACGGCAAAGATGGGAAAATCTTTGTCTGGAAAGGCAAGCAGGCAAACACGGAGGAGA
  GGAAGGCTGCCCTCAAAACAGCCTCTGACTTCATCACCAAGATGGACTACCCCAAGCAGACTCAGGTCTC
  GGTCCTTCCTGAGGGCGGTGAGACCCCACTGTTCAAGCAGTTCTTCAAGAACTGGCGGGACCCAGACCAG
  ACAGATGGCCTGGGCTTGTCCTACCTTTCCAGCCATATCGCCAACGTGGAGCGGGTGCCCTTCGACGCCG
  CCACCCTGCACACCTCCACTGCCATGGCCGCCCAGCACGGCATGGATGACGATGGCACAGGCCAGAAACA
  GATCTGGAGAATCGAAGGTTCCAACAAGGTGCCCGTGGACCCTGCCACATATGGACAGTTCTATGGAGGC
  GACAGCTACATCATTCTGTACAACTACCGCCATGGTGGCCGCCAGGGGCAGATAATCTATAACTGGCAGG
  GTGCCCAGTCTACCCAGGATGAGGTCGCTGCATCTGCCATCCTGACTGCTCAGCTGGATGAGGAGCTGGG
  AGGTACCCCTGTCCAGAGCCGTGTGGTCCAAGGCAAGGAGCCCGCCCACCTCATGAGCCTGTTTGGTGGG
  AAGCCCATGATCATCTACAAGGGCGGCACCTCCCGCGAGGGCGGGCAGACAGCCCCTGCCAGCACCCGCC
  TCTTCCAGGTCCGCGCCAACAGCGCTGGAGCCACCCGGGCTGTTGAGGTATTGCCTAAGGCTGGTGCACT
  GAACTCCAACGATGCCTTTGTTCTGAAAACCCCCTCAGCCGCCTACCTGTGGGTGGGTACAGGAGCCAGC
  GAGGCAGAGAAGACGGGGGCCCAGGAGCTGCTCAGGGTGCTGCGGGCCCAACCTGTGCAGGTGGCAGAAG
  GCAGCGAGCCAGATGGCTTCTGGGAGGCCCTGGGCGGGAAGGCTGCCTACCGCACATCCCCACGGCTGAA
  GGACAAGAAGATGGATGCCCATCCTCCTCGCCTCTTTGCCTGCTCCAACAAGATTGGACGTTTTGTGATC
  GAAGAGGTTCCTGGTGAGCTCATGCAGGAAGACCTGGCAACGGATGACGTCATGCTTCTGGACACCTGGG
  ACCAGGTCTTTGTCTGGGTTGGAAAGGATTCTCAAGAAGAAGAAAAGACAGAAGCCTTGACTTCTGCTAA
  GCGGTACATCGAGACGGACCCAGCCAATCGGGATCGGCGGACGCCCATCACCGTGGTGAAGCAAGGCTTT
  GAGCCTCCCTCCTTTGTGGGCTGGTTCCTTGGCTGGGATGATGATTACTGGTCTGTGGACCCCTTGGACA
  GGGCCATGGCTGAGCTGGCTGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198252 unedited
  GGCAGAATTTTGNTATACGACTCACTATAGGGCGGCCCGCGAATTCGCACCAGCAGCAGG
  TAGTGCTCATAGCTCTCTTTGTCCAGTGCTTCGGCCTTGGTCCCAGCGCCTTCCCACGGA
  GCAGCACTCTTCACCCTGCACAGCCTTGTTAGCCCAACAGCATGGTGGTGGAACACCCCG
  AGTTCCTCAAGGCAGGGAAGGAGCCTGGCCTGCAGATCTGGCGTGTGGAGAAGTTCGATC
  TGGTGCCCGTGCCCACCAACCTTTATGGAGACTTCTTCACGGGCGACGCCTACGTCATCC
  TGAAGACAGTGCAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACTACTGGCTGGGCA
  ATGAGTGCAGCCAGGATGAGAGCGGGGCGGCCGCCATCTTTACCGTGCAGCTGGATGACT
  ACCTGAACGGCCGGGCCGTGCAGCACCGTGAGGTCCAGGGCTTCGAGTCGGCCACCTTCC
  TAGGCTACTTCAAGTCTGGCCTGAAGTACAAGAAAGGAGGTGTGGCATCAGGATTCAAGC
  ACGTGGTACCCAACGAGGTGGTGGTGCAGAGACTCTTCCAGGTCAAAGGGCGGCGTGTGG
  TCCGTGCCACCGAGGTACCTGTGTCCTGGGAGAGCTTCAACAATGGCGACTGCTTCATCC
  TGGACCTGNGCAACAACATCCACCAGTGGTGTGGNTCCCAACAGCATCGGTATGAAAGAC
  TGAAGGCCACACAGTGTCCAAGGGCATCCGGGACAACGAGCGGAGTGGCCGGGCCCGAGT
  GCACGTGTCTGAGGAGGGCACTGAGCCCGAGCGATGCTCCCAGTGCTGGGCCCAAAGCCG
  CTCTGCTGCAGTACCCGAGACACGNCNNAGAGATGCGCCACCGCAGCTGCCAGCTCTACA
  GTCTCAATGTGCAGGACATGTGCGCTCCTCGGGCTGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198252
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198252.1, NP_937895.1
• RefSeq Size: 2685 bp
• RefSeq ORF: 2685 bp
• Locus ID: 2934
• Cytogenetics: 9q33.2
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter N-terminus compared to isoform a. Variants 2, 3, 5, 6, and 11-20 all encode isoform b.