PRMT1 (NM_198318) Human Untagged Clone

CAT#: SC107795

PRMT1 (untagged)-Human protein arginine methyltransferase 1 (PRMT1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRMT1
• Synonyms: ANM1; HCP1; HRMT1L2; IR1B4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107795 sequence for NM_198318 edited (data generated by NextGen Sequencing)
  ATGGCGGCAGCCGAGGCCGCGAACTGCATCATGGAGGTGTCCTGTGGCCAGGCGGAAAGC
  AGTGAGAAGCCCAACGCTGAGGACATGACATCCAAAGATTACTACTTTGACTCCTACGCA
  CACTTTGGCATCCACGAGGAGATGCTGAAGGACGAGGTGCGCACCCTCACTTACCGCAAC
  TCCATGTTTCATAACCGGCACCTCTTCAAGGACAAGGTGGTGCTGGACGTCGGCTCGGGC
  ACCGGCATCCTCTGCATGTTTGCTGCCAAGGCCGGGGCCCGCAAGGTCATCGGGATCGAG
  TGTTCCAGTATCTCTGATTATGCGGTGAAGATCGTCAAAGCCAACAAGTTAGACCACGTG
  GTGACCATCATCAAGGGGAAGGTGGAGGAGGTGGAGCTCCCAGTGGAGAAGGTGGACATC
  ATCATCAGCGAGTGGATGGGCTACTGCCTCTTCTACGAGTCCATGCTCAACACCGTGCTC
  TATGCCCGGGACAAGTGGCTGGCGCCCGATGGCCTCATCTTCCCAGACCGGGCCACGCTG
  TATGTGACGGCCATCGAGGACCGGCAGTACAAAGACTACAAGATCCACTGGTGGGAGAAC
  GTGTATGGCTTCGACATGTCTTGCATCAAAGATGTGGCCATTAAGGAGCCCCTAGTGGAT
  GTCGTGGACCCCAAACAGCTGGTCACCAACGCCTGCCTCATAAAGGAGGTGGACATCTAT
  ACCGTCAAGGTGGAAGACCTGACCTTCACCTCCCCGTTCTGCCTGCAAGTGAAGCGGAAT
  GACTACGTGCACGCCCTGGTGGCCTACTTCAACATCGAGTTCACACGCTGCCACAAGAGG
  ACCGGCTTCTCCACCAGCCCCGAGTCCCCGTACACGCACTGGAAGCAGACGGTGTTCTAC
  ATGGAGGACTACCTGACCGTGAAGACGGGCGAGGAGATCTTCGGCACCATCGGCATGCGG
  CCCAACGCCAAGAACAACCGGGACCTGGACTTCACCATCGACCTGGACTTCAAGGGCCAG
  CTGTGCGAGCTGTCCTGCTCCACCGACTACCGGATGCGCTGA
  
  Clone variation with respect to NM_198318.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198318 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCNGATTCGGCACGAGGGTGAAGATGGCGG
  CAGCCGAGGCCGCGAACTGCATCATGGAGGTGTCCTGTGGCCAGGCGGAAAGCAGTGAGA
  AGCCCAACGCTGAGGACATGACATCCAAAGATTACTACTTTGACTCCTACGCACACTTTG
  GCATCCACGAGGAGATGCTGAAGGACGAGGTGCGCACCCTCACTTACCGCAACTCCATGT
  TTCATAACCGGCACCTCTTCAAGGACAAGGTGGTGCTGGACGTCGGCTCGGGCACCGGCA
  TCCTCTGCATGTTTGCTGCCAAGGCCGGGGCCCGCAAGGTCATCGGGATCGAGTGTTCCA
  GTATCTCTGATTATGCGGTGAAGATCGTCAAAGCCAACAAGTTAGACCACGTGGTGACCA
  TCATCAAGGGGAAGGTGGAGGAGGTGGAGCTCCCAGTGGAGAAGGTGGACATCATCATCA
  GCGAGTGGATGGGCTACTGCCTCTTCTACGAGTCCATGCTCAACACCGTGCTCTATGCCC
  GGGACAAGTGGCTGGCGCCCGATGGCCTCATCTTCCCAGACCGGGCCACGCTGTATGTGA
  CGGCCATCGAGGACCGGCAGTACAAAGACCTACAGATCCACTGGTGGGAGAACGTGTATG
  GCTTCGACATGTCTTGCATANAGATGTGGCCATTANGGAGCCCCTAGTGGATGTCGTGGA
  CCCCCAACAGCTGGTCACCAACGCCTGCCTCATNANGGAGGTGGACATCTATACCGTCAG
  GTGGAAGACCTGACCTTTCACCTCCCCGTNNNCTGCTGCAGTGAAGCGAATGACTACGTG
  CACGCCCTGGTTGGCCTACTTCACATCGAGTTCACACGCTGCACAAGAGGACCGGCTTCT
  CCACAGNCCCGAGTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198318
• Insert Size: 1450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198318.1, NP_938074.1
• RefSeq Size: 1262 bp
• RefSeq ORF: 1032 bp
• Locus ID: 3276
• Cytogenetics: 19q13.33
• Gene Summary: 'This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (3) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.