E2F6 (NM_198257) Human Untagged Clone

CAT#: SC107802

E2F6 (untagged)-Human E2F transcription factor 6 (E2F6), transcript variant e

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: E2F6
• Synonyms: E2F-6; E2F-6, MGC111545; E2F transcription factor 6; E2F transcription factor 6, isoform 1; MGC111545
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107802 sequence for NM_198257 edited (data generated by NextGen Sequencing)
  ATGAGTCAGCAGCGGCCGGCGAGGAAGTTACCCAGTCTCCTCCTGGACCCGACGGAGGAG
  ACGGTTCGCCGTCGGTGCCGAGACCCCATCAACGTGGAGGGCCTGCTGAAGCTCTAA
  
  Clone variation with respect to NM_198257.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198257 unedited
  NGTTCGAATTTGTATACGACTCACTATAGGCGGCCCGCGATTCGGCACGAGGGAGAGGCG
  GGCCGGGCGACTTGAGATCCGCGGGTCTCCCTCCTCTTTTTCCGTCTGCGTCGGGAGCTC
  CCGGGCACGTGAGGCCGTGCCGCGTTTACTGGCGGGCGGGACGGCCTAGCCGGGCGGCGC
  CTCGGAGGAAGCCGCGGACCCCTTAGGTGCTGGGCCCTTGGAAATCGGCGCGTGGGGGGC
  GGTGCTCGAGCTGAGCGCGAGAGGGCGGGAGAGCTCGTGGGGTGCGAGGGGAGCAGGACG
  CCCGGCCGGGCAGCATGAGTCAGCAGCGGCCGGCGAGGAAGTTACCCAGTCTCCTCCTGG
  ACCCGACGGAGGAGACGGTTCGCCGTCGGTGCCGAGACCCCATCAACGTGGAGGGCCTGC
  TGAAGCTCTAAAAGTGAAGAGACCTCGTTTTGATGTATCGCTGGTTTATTTAACTCGAAA
  ATTTATGGATCTTGTCAGATCTGCTCCCGGGGGTATTCTTGACTTAAACAAGGTTGCAAC
  GAAACTGGGAGTCCGAAAGCGGAGAGTGTATGACATCACCAATGTCTTAGATGGAATCGA
  CCCTCGTTGAAAAGAAATCCAAGAACCATATTACATGGATAGGATCTGATCTTAGCAATT
  TTGGAGCAGTTCCCCACCAAAAGAAGCTACAGGAGGAACTTTCTGACTTATCAGCAATGG
  AAGATGCTTTGGATGAACTAATTAACGATTGTGCTCACCAGCTGTTTGAGTTACCAGATG
  ACCCAGCACAGGAAAGACTACTTATGTGACCTATCCAGACATTCTAGCCTTTANGCCTTC
  CATGAACAGATCGCCCTTTGCAAGTCAAGCTCCAGCCGAACCAGACTTGGGTGGTCCCAG
  CTCCCACAACAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_198257 unedited
  CTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTCAGACATTTATTGAGCACTTCTTAAGGG
  CCAGGCATGGTGTGAGGCACTGGGATGTAACAGTGATTATGACAAAGTCCCTCAAGGAGC
  TCACAGTCTAATGGTAAACTACAGAGTAAACTGAGGCACAGAGTGGTAAATGACTTACCC
  AATGAGGTAGAAACAGCTAGGTATGTGAAAATGTGGGAGCAGAGCTGGAGAGAGGGCAAG
  GTACAACTTGTCCTAAGTAAAACCCCTTGGACTGACTCAACAGCAAGGCTGCATACACAA
  ACATTACAAAATCTACTTGCAAAAACCTGAAAGGAGTTTCCAATTATCACCATGACTGAA
  TTCATGTTTCACAATGAATTCAGCTATTTCCTGATGGCATTTCTATCCTTGCCCCCAAAG
  CCACTGGGCATGCCACCAGTCTGCTGGGAGACAGCAATTAGGTAGACAGCTTAAAACCAA
  GAAGTATCTGACTCCAATTTTAGCACTGAAAACCATATTAAAATACTTTAATTAAATCCA
  CAAACATAAATGTGTAGCACATTTATGATACTTCTACATGTAGAAGTTTGGAAAAAGTCT
  CTTCTACCCTCATCCAGAGATTCTAATAAGTTTAGATACCAAGAGATAGAGAATTGTCTG
  CACTTTCATAATACATATTTTGCTGTCANGGGGAAAATGTACCTAAACTATTTCGAGTAT
  CTCACCTTTCCGATTATCCAAACTCCCTTTAATCCTGTGACCTTTTGCAAGTACAAAGTA
  CTGCAACGTGAACAGGGAAAGAATTCTTTGGGAAATGACATCCCCTGAGGAAGGTAAGCC
  CAAGAACGGCTTCCACCGACGTTTGTTCTAACTTGGAGAACTTTTAGGGCAATTCTTTCC
  TGACAACCTGATATTTAAGGGTTTTTATGGAACCCTTCTTATTTAGGGATCCAAAACTCG
  CCGAGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198257
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198257.1, NP_937988.1
• RefSeq Size: 2287 bp
• RefSeq ORF: 117 bp
• Locus ID: 1876
• Cytogenetics: 2p25.1
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]'
  Transcript Variant: This variant (e) lacks an alternate exon, compared to variant a, that causes a frameshift. The predicted isoform (3) is shorter at the N-terminus compared to isoform 1. Variants c, d, and e all encode isoform 3.