Dynein intermediate chain 1 (DNAI1) (NM_012144) Human Untagged Clone

CAT#: SC107912

DNAI1 (untagged)-Human dynein, axonemal, intermediate chain 1 (DNAI1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNAI1
• Synonyms: CILD1; DIC1; ICS1; PCD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012144, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATTCCTGCTTCTGCGAAGGCTCCCCATAAACAGCCTCATAAGCAGAGCATCAGCATAGGCAGAGGAA
  CCAGGAAGAGAGATGAAGATTCAGGGACTGAAGTGGGAGAAGGCACAGATGAATGGGCCCAATCCAAAGC
  CACAGTTAGACCCCCTGACCAGCTGGAGTTGACCGATGCGGAGTTAAAGGAGGAGTTCACTCGGATTTTG
  ACAGCCAACAACCCACACGCACCCCAGAACATTGTCAGGTACAGCTTCAAAGAAGGCACATATAAGCCTA
  TTGGCTTTGTGAACCAACTGGCAGTTCACTACACCCAGGTTGGGAACCTGATCCCCAAAGACTCAGATGA
  AGGACGGCGGCAGCATTACCGCGATGAATTAGTGGCAGGTTCTCAGGAGTCTGTCAAGGTGATTTCAGAA
  ACAGGAAACCTCGAAGAAGACGAAGAGCCCAAGGAGTTAGAAACTGAGCCTGGGAGTCAAACAGATGTGC
  CTGCAGCTGGGGCAGCTGAAAAAGTGACTGAAGAAGAATTGATGACTCCTAAGCAGCCCAAGGAGAGAAA
  GCTCACTAACCAGTTCAACTTCAGTGAGAGGGCCTCACAGACCTACAACAACCCTGTCCGGGATCGAGAA
  TGCCAGACGGAGCCTCCTCCCAGGACAAACTTTTCAGCCACAGCCAATCAGTGGGAGATCTATGATGCCT
  ATGTAGAGGAACTTGAGAAGCAGGAAAAGACCAAAGAGAAGGAGAAGGCAAAGACCCCAGTGGCTAAAAA
  ATCAGGGAAGATGGCCATGAGGAAGCTGACATCTATGGAGTCTCAGACTGATGATCTCATCAAATTGTCC
  CAAGCTGCTAAGATCATGGAGCGGATGGTCAACCAGAATACATATGATGACATTGCTCAAGATTTTAAGT
  ACTATGACGATGCTGCTGATGAATACCGGGACCAGGTGGGTACCCTGCTGCCGCTCTGGAAGTTCCAAAA
  TGACAAAGCCAAGCGCCTGTCCGTCACTGCCCTCTGCTGGAATCCAAAGTACAGGGATCTGTTTGCAGTG
  GGATATGGCTCTTATGACTTCATGAAGCAGAGCCGGGGCATGCTGCTGCTCTACAGCCTGAAGAACCCCA
  GCTTCCCTGAGTACATGTTCAGCAGCAACAGCGGCGTCATGTGTCTCGACATCCACGTGGACCACCCCTA
  CCTGGTGGCAGTAGGCCACTATGACGGCAACGTGGCCATTTACAACCTCAAGAAGCCCCACTCCCAGCCC
  TCCTTCTGCAGCTCAGCCAAGTCTGGCAAGCACTCAGACCCTGTGTGGCAGGTCAAGTGGCAGAAGGATG
  ACATGGACCAAAACCTTAACTTCTTCTCTGTGTCATCTGACGGCAGGATTGTGTCTTGGACTCTCGTGAA
  GAGAAAGCTGGTTCACATAGATGTCATCAAGCTGAAGGTGGAAGGCAGCACCACGGAAGTTCCTGAGGGG
  TTGCAGCTGCACCCAGTGGGTTGTGGCACTGCCTTTGACTTCCACAAAGAGATTGACTACATGTTCCTAG
  TGGGCACAGAGGAGGGAAAAATCTACAAGTGCTCTAAATCCTACTCCAGCCAATTCCTCGACACCTATGA
  CGCCCACAACATGTCAGTGGACACTGTGTCCTGGAACCCATACCACACCAAGGTCTTCATGTCCTGCAGC
  TCCGACTGGACAGTGAAGATCTGGGACCACACCATCAAGACCCCGATGTTCATCTATGACCTGAACTCAG
  CCGTGGGTGATGTGGCCTGGGCGCCATACTCTTCTACTGTGTTCGCAGCAGTCACCACAGATGGGAAGGC
  CCACATATTTGACTTAGCCATCAACAAGTATGAGGCCATCTGCAACCAGCCTGTGGCGGCCAAAAAGAAC
  AGGCTCACCCACGTGCAGTTCAATCTCATCCACCCCATCATCATTGTGGGCGATGACCGTGGGCACATCA
  TCAGCCTCAAGCTCTCACCCAATTTGCGCAAGATGCCAAAGGAAAAGAAGGGGCAGGAGGTGCAGAAGGG
  TCCAGCTGTGGAGATTGCGAAACTGGACAAACTGCTGAACCTGGTGAGGGAAGTGAAAATCAAGACCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012144 unedited
  TTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGACGGCTGTCCCTAAG
  AACCGTTGCGACTGGTAACTGAAGTGGAAGAGAGTCCAGATTTCTTGTGTGTGGTCAAGG
  AGACGGACAAACTTTTTGTCTTCAGACGAGGGAGCGTTTTGTAGGCTCTCCAGGGNGTTG
  AGATGATTCCTGCTTCTGCGAAGGCTCCCCATAAACAGCCTCATAAGCAGAGCATCAGCA
  TAGGCAGAGGAACCAGGAAGAGAGATGAAGATTCAGGGACTGAAGTGGGAGAAGGCACAG
  ATGAATGGGCCCAATCCAAAGCCACAGTTAGACCCCCTGACCAGCTGGAGTTGACCGATG
  CGGAGTTAAAGGAGGAGTTCACTCGGATTTTGACAGCCAACAACCCACACGCACCCCANA
  ACATTGTCAGGTACAGCTTCAAAAAGCAATGGAGTCTGGGTTTCATTCCTAAACTGAAGA
  GGAAGATCCCAGTCNACTACTGGGGACAAGATGAAGGTGAAATCTCCACTGAAACTGTAA
  GGGTAATTTATTCCTTGGGAATCTTCTTCTCAAAATATTTCCTCTTTTTTTTGGACCTTT
  TTTTTGANCCTTTTTCTTGTCGCATCTCTGGGGTTACTTGGTCCTGTTTTTGTTTTCCTT
  GCCTTTCTTGCCCTCCTTNNNCTCCCCCCCCCCCCGCCCTCCTGGCGTCTTTGTNTTTTT
  CTTTTTTTCTTCTTGTTTCTCCCCCCCCCCCTTGTTGCCCCCCCTCTTTTCCGGCTGCCC
  CCCGCCCGGTGTTNTTCCGCTCGCGTTCCCCCCCCCGCCCGCNTCTTTTCTGCCGCCGTC
  TTTNGTGGTTTATTTGTTGTTNGGNNGTGTGTGGCGNGTCGACGGATCGAAAAAAAAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012144 unedited
  CCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGGTTTCTACACAGGTTTATTTGT
  GCAAAGTTAGAGGGGTAATGGTGGTTGAAGACCAAGTCCTGGGGTAAGGTGCTGGGACCT
  TAAGGAACAGAACAAGAAGGGAAGATGCTGGGACCTGATCAGGAGTGGGGTCACATGCTG
  GGTGCTAAGGCTGGGCTGGGTACCAGGGTCAAGCCCTAGGAGTACTGTATTCAAGCGATG
  GGACAGAGACTGAGGCCAGCCCCTCAGGTCTTGATTTTCACTTCCCTCACCAGGTTCAGC
  AGTTTGTCCAGTTTCGCAATCTCCACAGCTGGACCCTTCTGCACCTTCTGCCCCTTCTTT
  TCCTTTGGCATCTTGCGCAAATTGGGTGAGAGCTTGAGGCTGATGATGTGCCCACGGTCA
  TCGCCCACAATGATGATGGGGTGGATGAGATTGAACTGCACGTGGGTGAGCCTGTTCTTT
  TTGCCCGCACAGGCTGGTTGCAGATGGGCTCATACTTGCTGATGGCTAAGTACATATGTG
  GGCCTTCCCATTTGGGTGACTGCTGTGAACACAGTAAAGACATGGCGCCCAGCCACATTA
  CCCAGGGTGAGTTACGCCATAGATAACCTCCGGTTTTGATGTTTCGGCCACATCTTATTG
  TCCACTCGACCCGAGACTTGACACCTTGTGTGTTGGGTTTTAGAACCCGGCTCACTGACG
  TTTGGGCCGTTCACGGGTCGCACCCCCCGTGCTTGATTTAAATCCCGCCCCCTGCGCCGC
  CGTTGTAAAATTGGTTATTCCCTCAGCGCCCTTATACGCCCTCTCTCGTCCCTTTTCTTC
  CTTCCCTTCCTTTTTTCCCCTTTCTACCCCCTTTTTCCTCCCCCCTCCCCCCTTTTCCCC
  CTCTCCTTTTCCTTCCCCCCCTTCCCCTCCCCCCCACTTTCTTCATTTTCTCTCCCTCTC
  CTCTCCCTCTTCCCCCACCTCTTCTCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012144
• ORF Size: 2100 bp
• Insert Size: 3520
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_012144.2, NP_036276.1
• RefSeq Size: 2548
• RefSeq ORF: 2100
• Locus ID: 27019
• Domains: WD40
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• Gene Summary: This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes isoform 1.