Collagen IX (COL9A1) (NM_078485) Human Untagged Clone

CAT#: SC107929

COL9A1 (untagged)-Human collagen, type IX, alpha 1 (COL9A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL9A1
• Synonyms: DJ149L1.1.2; EDM6; MED; STL4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107929 sequence for NM_078485 edited (data generated by NextGen Sequencing)
  ATGGCCTGGACTGCGCGGGACCGCGGGGCCCTGGGGCTGCTGCTGTTGGGGCTCTGCTTG
  TGCGCGGCTCAAAGAGGTCCCCCGGGTGAGCAGGGTCCTCCCGGGCCTCCGGGCCCCCCT
  GGAGTTCCAGGCATCGATGGCATCGACGGTGACCGAGGTCCTAAGGGCCCCCCGGGCCCC
  CCGGGTCCTGCAGGTGAACCGGGAAAGCCAGGAGCTCCAGGCAAGCCTGGCACACCTGGC
  GCTGATGGATTAACAGGACCTGATGGATCCCCTGGCTCCATTGGGTCAAAGGGACAAAAA
  GGAGAACCTGGTGTGCCTGGATCGCGTGGATTTCCAGGCCGTGGTATTCCTGGACCCCCT
  GGTCCTCCTGGGACAGCAGGACTCCCTGGAGAGCTTGGCCGTGTAGGACCTGTTGGTGAC
  CCTGGGAGAAGAGGACCACCTGGCCCCCCTGGCCCCCCAGGACCCAGAGGAACAATTGGC
  TTTCATGATGGAGATCCATTGTGTCCCAATGCCTGTCCACCAGGTCGCTCAGGATATCCA
  GGCCTACCAGGCATGAGGGGTCATAAAGGGGCTAAAGGAGAAATTGGTGAACCAGGAAGA
  CAAGGACACAAGGGTGAAGAAGGTGACCAGGGAGAACTCGGAGAAGTTGGAGCTCAAGGA
  CCTCCAGGAGCCCAGGGTTTGCGAGGCATCACCGGCATAGTTGGGGACAAAGGGGAAAAA
  GGTGCTCGGGGCTTAGATGGTGAACCTGGGCCTCAGGGTCTTCCTGGTGCACCTGGTGAT
  CAAGGACAGCGAGGACCTCCAGGAGAAGCAGGTCCCAAAGGAGATAGAGGGGCTGAAGGT
  GCTAGAGGAATTCCTGGTCTCCCTGGGCCCAAAGGAGACACGGGTTTGCCAGGTGTGGAT
  GGCCGTGATGGGATCCCTGGAATGCCTGGAACAAAGGGTGAACCAGGAAAACCTGGGCCT
  CCTGGTGATGCAGGATTGCAGGGGTTACCAGGTGTACCTGGAATTCCTGGTGCAAAGGGT
  GTTGCTGGTGAAAAGGGTAGCACAGGTGCTCCAGGGAAGCCTGGTCAGATGGGAAATTCA
  GGCAAACCGGGCCAACAGGGGCCTCCAGGAGAGGTGGGACCCCGAGGACCCCGGGGGCTT
  CCTGGCAGTAGAGGAGAATTAGGACCAGTGGGATCCCCAGGCCTACCAGGTAAACTGGGT
  TCTCTGGGTAGCCCTGGCCTCCCTGGCTTGCCTGGGCCCCCTGGACTTCCTGGAATGAAA
  GGTGACAGGGGTGTAGTCGGTGAACCGGGTCCAAAGGGTGAACAGGGTGCCTCTGGTGAA
  GAAGGTGAAGCAGGAGAAAGGGGGGAACTTGGAGATATAGGATTACCTGGCCCAAAGGGA
  TCTGCAGGTAATCCTGGGGAACCTGGCTTGAGAGGGCCTGAGGGAAGTCGGGGGCTTCCT
  GGAGTGGAAGGACCAAGAGGACCACCTGGACCCCGGGGTGTGCAGGGAGAACAGGGTGCC
  ACCGGCCTGCCTGGTGTCCAGGGCCCTCCGGGTAGAGCACCGACAGATCAGCACATTAAG
  CAGGTTTGCATGAGAGTCATACAAGAACATTTTGCTGAGATGGCTGCCAGTCTTAAGCGT
  CCAGACTCAGGTGCCACTGGGCTTCCTGGAAGGCCTGGCCCTCCTGGTCCCCCCGGCCCT
  CCTGGAGAGAATGGTTTCCCAGGCCAGATGGGAATTCGTGGCCTTCCGGGCATTAAGGGG
  CCCCCTGGTGCTCTTGGTTTGAGGGGACCTAAAGGTGACTTGGGAGAAAAGGGGGAGCGT
  GGCCCTCCAGGAAGAGGTCCCAACGGTTTGCCTGGAGCTATAGGTCTCCCAGGTGACCCA
  GGCCCTGCCAGCTATGGCAGAAATGGCCGAGACGGTGAGCGAGGCCCCCCAGGGGTGGCA
  GGAATTCCTGGAGTGCCTGGACCCCCGGGACCTCCTGGGCTTCCCGGTTTCTGTGAGCCA
  GCCTCCTGCACCATGCAGGCTGGTCAGCGAGCATTTAACAAAGGGCCTGACCCTTGA
  
  Clone variation with respect to NM_078485.3
  1133 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_078485 unedited
  TATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGCCACCTATGCTAGTGGCGG
  GTCTGGAAGCCTAGAGGGGAACCAGGCTGCAGAGCCGGGCCAAGGGATTAGCGGCGGGCG
  GCGGGCATGGCCTGGACTGCGCGGGACCGCGGGGCCCTGGGGCTGCTGCTGTTGGGGCTC
  TGCTTGTGCGCGGCTCAAAGAGGTCCCCCGGGTGAGCAGGGTCCTCCCGGGCCTCCGGGC
  CCCCCTGGAGTTCCAGGCATCGATGGCATCGACGGTGACCGAGGTCCTAAGCCCCCCCCC
  GGGCCCCCCGGGTCCTGCAGGTGAACCGGGAAAGCCAGGAGCTCCAGGCAAGCCTGGCAC
  ACCTGGCGCTGATGGATTAACAGGACCTGATGGATCCCCTGGCTCCATTGGGTCAAAGGG
  ACAAAAAGGAGAACCTGGTGTGCCTGGATCGCGTGGATTTCCAGGCCGTGGTATTCCTGG
  ACCCCCTGGTCCTCCTGGGACAGCAGGACTCCCTGGAGAGCTTGGCCGTGTAGGACCTGG
  TTGGTGACCCTGGGAGAAGAGGACCACCTGGNCCCCCCTGGCCCNCCAGGACCCAGAGGA
  ACAATTGGCTTTCATGATGGGAGATCCANTTGTGTCCCAATGCCTGTCCACCANGTCGCT
  CAGGATATCCAGGCCTACCNGCATGGGGGGGTCATAAGGGGCTAAAGGAGAAATTGGGTG
  ACCANGAAAGACAGGACACAAGGTGGAAGAAAGTGACCAGGGAGACTCCGAGAAGTTGGA
  GCTCAAGGACCTCCAGAGCCCAGGGTTTGGCGAGCATCACGGCNATATTGGGGACAAGGG
  GAAAAAGGTGCTCGGGCTTTAAAGGGGGAACCTGGGCCTTAGGTCTTNCTGGGGCCCTGG
  GGATCAAGGACGCGAGGACCTCCGAAAACCAGGCCCAAGGAAATAAAGGGCTTGAGGGCT
  TAAGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_078485 unedited
  GGTCCGACTATGNACCGCGGNCCGCATNCTANGATCGNGTTTTTTTTTTTTTTTTTTTTA
  GTTAAGTAATGTTTAATGTATCTAAATAAGCTCAGTAACTTCTTTTCATTGAACATGATT
  ATTTATAAGAAAACATGCAAGAGATTAAGAAACCATATTTATTTCTTCTTTGGTACAACC
  AGAGATGTTACCTCAAACCTTTAAAATAAATAATCTCATCAATAAAATTTATGAATGCCA
  GAGTATTAAAATTATTTCTTTGCTTTCACATAGAAGCGCTCAAAATATTAACAGGTTTGG
  GGTAAAGTACATACAGTTCAACAAAAGTTCAAGGTGAAGAAGGAAACCAATACATGACGT
  AAAGAAATCATTTCGGGAGATGTAGCCCTTTCTTATCACTCCAGAGACAGCACATCGATG
  AGATTTTAAGCACCAATTCCATTGAATGTAGTGGGGTTTCTTCATAACTAGTCTAGTAAT
  CCTCTATATAACTGAAACAATAATCTGAGAACAACTTCTAAATGAAAATAACTACTGCAA
  CTGAGGTAAACCAAACAGTTGTTTTTGTTTATTGGCACAGTGGCCCACGATAAGACACAT
  AGGGTTAGCACACTAAAGCTCAAGATCCTGGGAACAGGAGTATAAAATTGTTCAAGGGAG
  GTGGTTGGTTTTCTTTTTTTTTTTTTAACTGATGACTCTGCTGTCTTCCCTCCCAGGGAA
  AGGAAAAAAAACTTACTGAATTAGCCCCGTTCTCTATATGTGATTGGCCAGTAGGACTTC
  TGTAATCCTACTGGAGGGAAAACATTGTAAGCAGGCCGAAGGGAAACATCTTTGCCCCCA
  CTTTGGAAGGGGGTTTTTTAACCAAGCTTCTTTACCACAGGGTGGTTTATGGCCAACGGC
  TTGCGGCAGTAAACCTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_078485
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_078485.2, NP_511040.2
• RefSeq Size: 2985 bp
• RefSeq ORF: 2037 bp
• Locus ID: 1297
• Cytogenetics: 6q13
• Domains: Collagen
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks several exons in the 5' coding region, compared to variant 1. These differences cause translation initiation at a unique AUG and an isoform (2, also known as the short form) with a shorter N-terminus, compared to isoform 1.