Alkaline Phosphatase (ALPL) (NM_000478) Human Untagged Clone

CAT#: SC107943

ALPL (untagged)-Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALPL
• Synonyms: AP-TNAP; APTNAP; HOPS; TNALP; TNAP; TNSALP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107943 sequence for NM_000478 edited (data generated by NextGen Sequencing)
  ATGATTTCACCATTCTTAGTACTGGCCATTGGCACCTGCCTTACTAACTCCTTAGTGCCA
  GAGAAAGAGAAAGACCCCAAGTACTGGCGAGACCAAGCGCAAGAGACACTGAAATATGCC
  CTGGAGCTTCAGAAGCTCAACACCAACGTGGCTAAGAATGTCATCATGTTCCTGGGAGAT
  GGGATGGGTGTCTCCACAGTGACGGCTGCCCGCATCCTCAAGGGTCAGCTCCACCACAAC
  CCTGGGGAGGAGACCAGGCTGGAGATGGACAAGTTCCCCTTCGTGGCCCTCTCCAAGACG
  TACAACACCAATGCCCAGGTCCCTGACAGCGCCGGCACCGCCACCGCCTACCTGTGTGGG
  GTGAAGGCCAATGAGGGCACCGTGGGGGTAAGCGCAGCCACTGAGCGTTCCCGGTGCAAC
  ACCACCCAGGGGAACGAGGTCACCTCCATCCTGCGCTGGGCCAAGGACGCTGGGAAATCT
  GTGGGCATTGTGACCACCACGAGAGTGAACCATGCCACCCCCAGCGCCGCCTACGCCCAC
  TCGGCTGACCGGGACTGGTACTCAGACAACGAGATGCCCCCTGAGGCCTTGAGCCAGGGC
  TGTAAGGACATCGCCTACCAGCTCATGCATAACATCAGGGACATTGACGTGATCATGGGG
  GGTGGCCGGAAATACATGTACCCCAAGAATAAAACTGATGTGGAGTATGAGAGTGACGAG
  AAAGCCAGGGGCACGAGGCTGGACGGCCTGGACCTCGTTGACACCTGGAAGAGCTTCAAA
  CCGAGATACAAGCACTCCCACTTCATCTGGAACCGCACGGAACTCCTGACCCTTGACCCC
  CACAATGTGGACTACCTATTGGGTCTCTTCGAGCCAGGGGACATGCAGTACGAGCTGAAC
  AGGAACAACGTGACGGACCCGTCACTCTCCGAGATGGTGGTGGTGGCCATCCAGATCCTG
  CGGAAGAACCCCAAAGGCTTCTTCTTGCTGGTGGAAGGAGGCAGAATTGACCACGGGCAC
  CATGAAGGAAAAGCCAAGCAGGCCCTGCATGAGGCGGTGGAGATGGACCGGGCCATCGGG
  CAGGCAGGCAGCTTGACCTCCTCGGAAGACACTCTGACCGTGGTCACTGCGGACCATTCC
  CACGTCTTCACATTTGGTGGATACACCCCCCGTGGCAACTCTATCTTTGGTCTGGCCCCC
  ATGCTGAGTGACACAGACAAGAAGCCCTTCACTGCCATCCTGTATGGCAATGGGCCTGGC
  TACAAGGTGGTGGGCGGTGAACGAGAGAATGTCTCCATGGTGGACTATGCTCACAACAAC
  TACCAGGCGCAGTCTGCTGTGCCCCTGCGCCACGAGACCCACGGCGGGGAGGACGTGGCC
  GTCTTCTCCAAGGGCCCCATGGCGCACCTGCTGCACGGCGTCCACGAGCAGAACTACGTC
  CCCCACGTGATGGCGTATGCAGCCTGCATCGGGGCCAACCTCGGCCACTGTGCTCCTGCC
  AGCTCGGCAGGCAGCCTTGCTGCAGGCCCCCTGCTGCTCGCGCTGGCCCTCTACCCCCTG
  AGCGTCCTGTTCTGA
  
  Clone variation with respect to NM_000478.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000478 unedited
  CATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGCAGCTGAG
  AATGGCCCAGGNAAAGAACTATATTACCTTCAAAAAGAGAGGTACATGCGATGTTTGAGG
  TGGCATGAAGCTCAGTGGTGTTATATTGGAATGAGTGAGTGACCATCCTGGAGCCTTCCT
  GAAAGAGGATTGGAACATCAGTTAACATCTGACCACTGCCAGCCCACCCCCTCCCACCCA
  CGTCGATTGCATCTCTGGGCTCCAGGGATAAAGCAGGTCTTGGGGTGCACCATGATTTCA
  CCATTCTTAGTACTGGCCATTGGCACCTGCCTTACTAACTCCTTAGTGCCAGAGAAAGAG
  AAAGACCCCAAGTACTGGCGAGACCAAGCGCAAGAGACACTGAAATATGCCCTGGAGCTT
  CAGAAGCTCAACACCAACGTGGCTAAGAATGTCATCATGTTCCTGGGAGATGGGATGGGT
  GTCTCCACAGTGACGGCTGCCCGCATCCTCAAGGGTCAGCTCCACCACAACCCTGGGGAG
  GAGACCAGGCTGGAGATGGACAAGTTCCCCTTCGTGGCCCTCTCCAAGACGTACAACACC
  AATGCCCAGGTCCCTGACAGCGCCGGCACCGCCACCGCCTACCTGTGTGGGGTGAAGGCC
  AATGAGGGCACCGTGGGGGTAAGCGCAGCCACTGAGCGTTCCCGGTGCAACACCACCCAG
  GGGAACGAGGTCACCTCCATCCTGCGCTNGGCCAAGGACGCTGGGAAATCTGTGGGCATT
  GTGACCACCACGAGAGTGAACCATGCCACCCCCAGCGCCGCCTACGCCCACTCGGNTGAC
  CGGNACTGGTACTCAGACACGAGATGCCCCCTGTAGCCCTGAGCCAGGGCTGTTAAGACA
  TCGCCTACAGCTCATGCATACATCAGGGACATGACGTGACTTGGGGGGTGGCCGCAATAC
  TGTACCCCAGATAAACTATGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000478 unedited
  NTTTTACTCTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTATTTGGAAAGTTTTATTTAAATGTTTTGGGTTCCCTTTTAACCACCACCAA
  AAAAAAAAAAAAAAATTTTTTTTTGCCGTTCCAAACAGGAAAGTCGCTTCAAAGAGGAGC
  TGGCGTGTGTGTTCAAAGCCCAGGAGTGTGAGCCCTGCCAGGTGGCTCTTCCCGGCCTGG
  GCCACGCTGGGTAGGATGGCCACCCAGGCACCGGCTTCCTGGGACCCTGGTGGTCTTGGA
  GTGAGTGAGTGACCAAGGCCTTGTCCTGAGGAAAAAGACCTCAACTCCCCTGAAGGAGGG
  CTGGGCCTGGGGACAGGGACATGAGCATTGTAGGGCCTGGGGGGTTCCAAAGGTAAGGAA
  ATGGGAGTGGGAGGGTCAGGAAATGAAACTGGGAGGGTCAAATCCAAAATGTTCCACGGA
  GGCTTCAGGGCCTCTGGGTCTGGAGAAATACGTTCGCTAAAAAAATAAAAGGCTTTGAAA
  ATGTCTGCAGTCTGTACTCTCTGCCTGCCCAAGAGAAATCTACCCTGTTGGCCAAGGGCA
  NCGGGGAGGGAGCAAAGGCTGGAGGCCAAAAGTGGGTTTGGCCCTTGGGGAAGATTCCAN
  AGGGGAGCGAGGTGGGCGGCAAACTTTGGTTTCCTGGGTCCCCTTTCTTGCAGTTGCAGA
  GGCTTGAGAGGCCCCCACCTNCCTGCCCCTTGAGGAGGGGGGCTGCCGTGTGNGGAAAGT
  GGCATCTGTCACGGGCTTGTGGGGTGCCCGGGCCCTGNGCCCTCAAACAGGACGCTCAGG
  GGNTANAGGCCANCGCGAGCNCAGGGGGCCTGCANCAAGGCTGCTGCCGAGCTGCAGAAC
  ACATGGNCCGAGTTGCCCCGAGCAGCTGATCCCATCCTGGGGACACTATTTTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000478
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000478.2, NP_000469.2
• RefSeq Size: 2580 bp
• RefSeq ORF: 1575 bp
• Locus ID: 249
• Cytogenetics: 1p36.12
• Domains: alk_phosphatase
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).