Alkaline Phosphatase (ALPL) (NM_000478) Human Untagged Clone

CAT#: SC107943

ALPL (untagged)-Human alkaline phosphatase, liver/bone/kidney (ALPL), transcript variant 1


  "NM_000478" in other vectors (7)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ALPL
Synonyms AP-TNAP; APTNAP; HOPS; TNALP; TNAP; TNSALP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC107943 sequence for NM_000478 edited (data generated by NextGen Sequencing)
ATGATTTCACCATTCTTAGTACTGGCCATTGGCACCTGCCTTACTAACTCCTTAGTGCCA
GAGAAAGAGAAAGACCCCAAGTACTGGCGAGACCAAGCGCAAGAGACACTGAAATATGCC
CTGGAGCTTCAGAAGCTCAACACCAACGTGGCTAAGAATGTCATCATGTTCCTGGGAGAT
GGGATGGGTGTCTCCACAGTGACGGCTGCCCGCATCCTCAAGGGTCAGCTCCACCACAAC
CCTGGGGAGGAGACCAGGCTGGAGATGGACAAGTTCCCCTTCGTGGCCCTCTCCAAGACG
TACAACACCAATGCCCAGGTCCCTGACAGCGCCGGCACCGCCACCGCCTACCTGTGTGGG
GTGAAGGCCAATGAGGGCACCGTGGGGGTAAGCGCAGCCACTGAGCGTTCCCGGTGCAAC
ACCACCCAGGGGAACGAGGTCACCTCCATCCTGCGCTGGGCCAAGGACGCTGGGAAATCT
GTGGGCATTGTGACCACCACGAGAGTGAACCATGCCACCCCCAGCGCCGCCTACGCCCAC
TCGGCTGACCGGGACTGGTACTCAGACAACGAGATGCCCCCTGAGGCCTTGAGCCAGGGC
TGTAAGGACATCGCCTACCAGCTCATGCATAACATCAGGGACATTGACGTGATCATGGGG
GGTGGCCGGAAATACATGTACCCCAAGAATAAAACTGATGTGGAGTATGAGAGTGACGAG
AAAGCCAGGGGCACGAGGCTGGACGGCCTGGACCTCGTTGACACCTGGAAGAGCTTCAAA
CCGAGATACAAGCACTCCCACTTCATCTGGAACCGCACGGAACTCCTGACCCTTGACCCC
CACAATGTGGACTACCTATTGGGTCTCTTCGAGCCAGGGGACATGCAGTACGAGCTGAAC
AGGAACAACGTGACGGACCCGTCACTCTCCGAGATGGTGGTGGTGGCCATCCAGATCCTG
CGGAAGAACCCCAAAGGCTTCTTCTTGCTGGTGGAAGGAGGCAGAATTGACCACGGGCAC
CATGAAGGAAAAGCCAAGCAGGCCCTGCATGAGGCGGTGGAGATGGACCGGGCCATCGGG
CAGGCAGGCAGCTTGACCTCCTCGGAAGACACTCTGACCGTGGTCACTGCGGACCATTCC
CACGTCTTCACATTTGGTGGATACACCCCCCGTGGCAACTCTATCTTTGGTCTGGCCCCC
ATGCTGAGTGACACAGACAAGAAGCCCTTCACTGCCATCCTGTATGGCAATGGGCCTGGC
TACAAGGTGGTGGGCGGTGAACGAGAGAATGTCTCCATGGTGGACTATGCTCACAACAAC
TACCAGGCGCAGTCTGCTGTGCCCCTGCGCCACGAGACCCACGGCGGGGAGGACGTGGCC
GTCTTCTCCAAGGGCCCCATGGCGCACCTGCTGCACGGCGTCCACGAGCAGAACTACGTC
CCCCACGTGATGGCGTATGCAGCCTGCATCGGGGCCAACCTCGGCCACTGTGCTCCTGCC
AGCTCGGCAGGCAGCCTTGCTGCAGGCCCCCTGCTGCTCGCGCTGGCCCTCTACCCCCTG
AGCGTCCTGTTCTGA

Clone variation with respect to NM_000478.4
>OriGene 5' read for NM_000478 unedited
CATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGCAGCTGAG
AATGGCCCAGGNAAAGAACTATATTACCTTCAAAAAGAGAGGTACATGCGATGTTTGAGG
TGGCATGAAGCTCAGTGGTGTTATATTGGAATGAGTGAGTGACCATCCTGGAGCCTTCCT
GAAAGAGGATTGGAACATCAGTTAACATCTGACCACTGCCAGCCCACCCCCTCCCACCCA
CGTCGATTGCATCTCTGGGCTCCAGGGATAAAGCAGGTCTTGGGGTGCACCATGATTTCA
CCATTCTTAGTACTGGCCATTGGCACCTGCCTTACTAACTCCTTAGTGCCAGAGAAAGAG
AAAGACCCCAAGTACTGGCGAGACCAAGCGCAAGAGACACTGAAATATGCCCTGGAGCTT
CAGAAGCTCAACACCAACGTGGCTAAGAATGTCATCATGTTCCTGGGAGATGGGATGGGT
GTCTCCACAGTGACGGCTGCCCGCATCCTCAAGGGTCAGCTCCACCACAACCCTGGGGAG
GAGACCAGGCTGGAGATGGACAAGTTCCCCTTCGTGGCCCTCTCCAAGACGTACAACACC
AATGCCCAGGTCCCTGACAGCGCCGGCACCGCCACCGCCTACCTGTGTGGGGTGAAGGCC
AATGAGGGCACCGTGGGGGTAAGCGCAGCCACTGAGCGTTCCCGGTGCAACACCACCCAG
GGGAACGAGGTCACCTCCATCCTGCGCTNGGCCAAGGACGCTGGGAAATCTGTGGGCATT
GTGACCACCACGAGAGTGAACCATGCCACCCCCAGCGCCGCCTACGCCCACTCGGNTGAC
CGGNACTGGTACTCAGACACGAGATGCCCCCTGTAGCCCTGAGCCAGGGCTGTTAAGACA
TCGCCTACAGCTCATGCATACATCAGGGACATGACGTGACTTGGGGGGTGGCCGCAATAC
TGTACCCCAGATAAACTATGT
>OriGene 3' read for NM_000478 unedited
NTTTTACTCTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTATTTGGAAAGTTTTATTTAAATGTTTTGGGTTCCCTTTTAACCACCACCAA
AAAAAAAAAAAAAAATTTTTTTTTGCCGTTCCAAACAGGAAAGTCGCTTCAAAGAGGAGC
TGGCGTGTGTGTTCAAAGCCCAGGAGTGTGAGCCCTGCCAGGTGGCTCTTCCCGGCCTGG
GCCACGCTGGGTAGGATGGCCACCCAGGCACCGGCTTCCTGGGACCCTGGTGGTCTTGGA
GTGAGTGAGTGACCAAGGCCTTGTCCTGAGGAAAAAGACCTCAACTCCCCTGAAGGAGGG
CTGGGCCTGGGGACAGGGACATGAGCATTGTAGGGCCTGGGGGGTTCCAAAGGTAAGGAA
ATGGGAGTGGGAGGGTCAGGAAATGAAACTGGGAGGGTCAAATCCAAAATGTTCCACGGA
GGCTTCAGGGCCTCTGGGTCTGGAGAAATACGTTCGCTAAAAAAATAAAAGGCTTTGAAA
ATGTCTGCAGTCTGTACTCTCTGCCTGCCCAAGAGAAATCTACCCTGTTGGCCAAGGGCA
NCGGGGAGGGAGCAAAGGCTGGAGGCCAAAAGTGGGTTTGGCCCTTGGGGAAGATTCCAN
AGGGGAGCGAGGTGGGCGGCAAACTTTGGTTTCCTGGGTCCCCTTTCTTGCAGTTGCAGA
GGCTTGAGAGGCCCCCACCTNCCTGCCCCTTGAGGAGGGGGGCTGCCGTGTGNGGAAAGT
GGCATCTGTCACGGGCTTGTGGGGTGCCCGGGCCCTGNGCCCTCAAACAGGACGCTCAGG
GGNTANAGGCCANCGCGAGCNCAGGGGGCCTGCANCAAGGCTGCTGCCGAGCTGCAGAAC
ACATGGNCCGAGTTGCCCCGAGCAGCTGATCCCATCCTGGGGACACTATTTTGC
Restriction Sites NotI-NotI     
ACCN NM_000478
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000478.2, NP_000469.2
RefSeq Size 2580 bp
RefSeq ORF 1575 bp
Locus ID 249
Cytogenetics 1p36.12
Domains alk_phosphatase
Protein Families Druggable Genome
Protein Pathways Folate biosynthesis, Metabolic pathways
Gene Summary 'This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]'
Transcript Variant: This variant (1) encodes the longest isoform (1).

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