Cytochrome P450 2C8 (CYP2C8) (NM_000770) Human Untagged Clone

CAT#: SC107944

CYP2C8 (untagged)-Human cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP2C8
• Synonyms: CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107944 sequence for NM_000770 edited (data generated by NextGen Sequencing)
  ATGGAACCTTTTGTGGTCCTGGTGCTGTGTCTCTCTTTTATGCTTCTCTTTTCACTCTGG
  AGACAGAGCTGTAGGAGAAGGAAGCTCCCTCCTGGCCCCACTCCTCTTCCTATTATTGGA
  AATATGCTACAGATAGATGTTAAGGACATCTGCAAATCTTTCACCAATTTCTCAAAAGTC
  TATGGTCCTGTGTTCACCGTGTATTTTGGCATGAATCCCATAGTGGTGTTTCATGGATAT
  GAGGCAGTGAAGGAAGCCCTGATTGATAATGGAGAGGAGTTTTCTGGAAGAGGCAATTCC
  CCAATATCTCAAAGAATTACTAAAGGACTTGGAATCATTTCCAGCAATGGAAAGAGATGG
  AAGGAGATCCGGCGTTTCTCCCTCACAACCTTGCGGAATTTTGGGATGGGGAAGAGGAGC
  ATTGAGGACCGTGTTCAAGAGGAAGCTCACTGCCTTGTGGAGGAGTTGAGAAAAACCAAG
  GCTTCACCCTGTGATCCCACTTTCATCCTGGGCTGTGCTCCCTGCAATGTGATCTGCTCC
  GTTGTTTTCCAGAAACGATTTGATTATAAAGATCAGAATTTTCTCACCCTGATGAAAAGA
  TTCAATGAAAACTTCAGGATTCTGAACTCCCCATGGATCCAGGTCTGCAATAATTTCCCT
  CTACTCATTGATTGTTTCCCAGGAACTCACAACAAAGTGCTTAAAAATGTTGCTCTTACA
  CGAAGTTACATTAGGGAGAAAGTAAAAGAACACCAAGCATCACTGGATGTTAACAATCCT
  CGGGACTTTATCGATTGCTTCCTGATCAAAATGGAGCAGGAAAAGGACAACCAAAAGTCA
  GAATTCAATATTGAAAACTTGGTTGGCACTGTAGCTGATCTATTTGTTGCTGGAACAGAG
  ACAACAAGCACCACTCTGAGATATGGACTCCTGCTCCTGCTGAAGCACCCAGAGGTCACA
  GCTAAAGTCCAGGAAGAGATTGATCATGTAATTGGCAGACACAGGAGCCCCTGCATGCAG
  GATAGGAGCCACATGCCTTACACTGATGCTGTAGTGCACGAGATCCAGAGATACAGTGAC
  CTTGTCCCCACCGGTGTGCCCCATGCAGTGACCACTGATACTAAGTTCAGAAACTACCTC
  ATCCCCAAGGGCACAACCATAATGGCATTACTGACTTCCGTGCTACATGATGACAAAGAA
  TTTCCTAATCCAAATATCTTTGACCCTGGCCACTTTCTAGATAAGAATGGCAACTTTAAG
  AAAAGTGACTACTTCATGCCTTTCTCAGCAGGAAAACGAATTTGTGCAGGAGAAGGACTT
  GCCCGCATGGAGCTATTTTTATTTCTAACCACAATTTTACAGAACTTTAACCTGAAATCT
  GTTGATGATTTAAAGAACCTCAATACTACTGCAGTTACCAAAGGGATTGTTTCTCTGCCA
  CCCTCATACCAGATCTGCTTCATCCCTGTCTGA
  
  Clone variation with respect to NM_000770.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000770 unedited
  GTCGGAATTTGTATACGACTCCTATAGGCGGCCGCCTAATTAGCAGGGAGTGTTATAAAA
  ACTTTGGAGTGCAAGCTCACAGCTGTCTTAATAAGAAGAGAAGGCTTCAATGGAACCTTT
  TGTGGTCCTGGTGCTGTGTCTCTCTTTTATGCTTCTCTTTTCACTCTGGAGACAGAGCTG
  TAGGAGAAGGAAGCTCCCTCCTGGCCCCACTCCTCTTCCTATTATTGGAAATATGCTACA
  GATAGATGTTAAGGACATCTGCAAATCTTTCACCAATTTCTCAAAAGTCTATGGTCCTGT
  GTTCACCGTGTATTTTGGCATGAATCCCATAGTGGTGTTTCATGGATATGAGGCAGTGAA
  GGAAGCCCTGATTGATAATGGAGAGGAGTTTTCTGGAAGAGGCAATTCCCCAATATCTCA
  AAGAATTACTAAAGGACTTGGAATCATTTCCAGCAATGGAAAGAGATGGAAGGAGATCCG
  GCGTTTCTCCCTCACAACCTTGCGGAATTTTGGGATGGGGAAGAGGAGCATTGAGGACCG
  TGTTCAAGAGGAAGCTCACTGCCTTGTGGAGGAGTTGAGAAAAACCAAGGCTTCACCCTG
  TGATCCCACTTTCATCCTGGGCTGTGCTCCCTGCAATGTGATCTGTTCCCGNTGTTTTCC
  AGAAACGATTTGATTATTAAGATCCAGATTTTCTCACCCTGATGAAAAGATTCAATGAAA
  ACTTCAGGATTCTGAACTCCCCATGGATCCAGGTCTGNCATAATTTCCCTCTACCATTGG
  ATTGTTTCCCAGGAACTCACAACANAGTGCTTTAAAAATGTTGCTCTTACACCGAAGNTA
  CATTAGGNNAGAAAGGTAAAAGAACACCAGGCATCCCTGGATGTTAACAATCCTCGGGAC
  TTAATCGATTGCTTCTGATCAAATGGAGCAGGGAAAGGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000770 unedited
  TTTAGCTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTCA
  GCAATTAATAACACTTTTTATTTAGCACATTCACAAAAAAAATGGATCTAAATTATCATT
  CATTAATCACTTTTCTGTGTTTTACAGGGATAACATATTAAAAAGTCAGCATTAGAAAAG
  TATTAGCATATGCAGCAATTAATACAAGTGTTACAGAGTATGAATAATTGCAGATATATT
  TGTGCAGTGACCTGAACAACTCTCCTTAATGGAGTTTGGATGCTTATGGGATATTGAGTG
  AATGGGAAGATTTGATGAGAGGTCAGAGAAGACATAATAGTGGGAATGTCCTTGATAAAA
  AAAGAGTTGCAGGTGATAGCAGATCGGCAGCCAGATGGGCTAGCATTCTTCAGACAGGGA
  TGAAGCAGATCTGGTATGAGGGTGGCAGAGAAACAATCCCTTTGGTAACTGCAGTAGTAT
  TGAGGTTCTTTAAATCATCAACAGATTTCAGGTTAAAGTTCTGTAAAATTGTGGTTAGAA
  ATAAAAATAGCTCCATGCGGGCAAGTCCTTCTCCTGCACAAATTCGTTTTCCTGCTGAGA
  AAGGCATGAAGTAGTCACTTTTCTTAAAGTTGCCATTCTTATCTAGAAAGTGGCCAGGGT
  CAAAGATATTTGGATTAGGAAATTCTTTGTCATCATGTAGCACGGGAAGTCAGTAATGCC
  ATTANTGGTTGTGCCCTTGNGGATGANGGTAGTTTCTGAACTTAGTATCAGTGGTCACTG
  CATGGGGCACACCGGTGGGGACAAGGTCACTGTATCTCTGGATCTCGTGCACTAAGCATC
  AGTGTAGGCATGTGGGCTCTTCCTGCTGCAGGGCTTCTGTGTCTGCATTACTGATCATCT
  CTTCTGACTTTACTGGACCTCTGGTGCTCACAGAACAGAGTCTTTCAAAAGGGGCCTTGT
  GGGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000770
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000770.2, NP_000761.2
• RefSeq Size: 1851 bp
• RefSeq ORF: 1473 bp
• Locus ID: 1558
• Cytogenetics: 10q23.33
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (1) encodes the longest isoform (a).