PAK1 (NM_002576) Human Untagged Clone

CAT#: SC107950

PAK1 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAK1
• Synonyms: IDDMSSD; PAKalpha
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002576 edited
  TCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTA
  TATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCG
  GCCGCGAATTCGGCACGAGGCACCGCCGGGAGGAGGAGGAGGAGCCGAGAGGAGCTGAGC
  GAGCGCGGAAGTAGCTGCTGCTGGTGGTGACAATGTCAAATAACGGCCTAGACATTCAAG
  ACAAACCCCCAGCCCCTCCGATGAGAAATACCAGCACTATGATTGGAGCCGGCAGCAAAG
  ATGCTGGAACCCTAAACCATGGTTCTAAACCTCTGCCTCCAAACCCAGAGGAGAAGAAAA
  AGAAGGACCGATTTTACCGATCCATTTTACCTGGAGATAAAACAAATAAAAAGAAAGAGA
  AAGAGCGGCCAGAGATTTCTCTCCCTTCAGATTTTGAACACACAATTCATGTCGGTTTTG
  ATGCTGTCACAGGGGAGTTTACGGGAATGCCAGAGCAGTGGGCCCGCTTGCTTCAGACAT
  CAAATATCACTAAGTCGGAGCAGAAGAAAAACCCGCAGGCTGTTCTGGATGTGTTGGAGT
  TTTACAACTCGAAGAAGACATCCAACAGCCAGAAATACATGAGCTTTACAGATAAGTCAG
  CTGAGGATTACAATTCTTCTAATGCCTTGAATGTGAAGGCTGTGTCTGAGACTCCTGCAG
  TGCCACCAGTTTCAGAAGATGAGGATGATGATGATGATGATGCTACCCCACCACCAGTGA
  TTGCTCCACGCCCAGAGCACACAAAATCTGTATACACACGGTCTGTGATTGAACCACTTC
  CTGTCACTCCAACTCGGGACGTGGCTACATCTCCCATTTCACCTACTGAAAATAACACCA
  CTCCACCAGATGCTTTGACCCGGAATACTGAGAAGCAGAAGAAGAAGCCTAAAATGTCTG
  ATGAGGAGATCTTGGAGAAATTACGAAGCATAGTGAGTGTGGGCGATCCTAAGAAGAAAT
  ATACACGGTTTGAGAAGATTGGACAAGGTGCTTCAGGCACCGTGTACACAGCAATGGATG
  TGGCCACAGGACAGGAGGTGGCCATTAAGCAGATGAATCTTCAGCTAAAGAGCTGCTACA
  GCATCAATTCCTGAAGATTGCCAAGCCCCTCTCCAGCCTCACTCCACTGATTGCTGCAGC
  TAAGGAGGCAACAAAGAACAATCACTAAAACCACACTCACCCCAGCCTCATTGTGCCAAG
  CCTTCTGTGAGATAAATGCACATTTCAGAAATTCCAACTCCTGATGCCCTCTTCTCCTTG
  CCTTGCTTCTCCCATTTCCTGATCTAGCACTCCTCAAGACTTTGATCCTTGGAAACCGTG
  TGTCCAGCATTGAAGAGAACTGCAACTGAATGACTAATCAGATGATGGCCATTTCTAAAT
  AAGGAATTTCCTCCCAATTCATGGATATGAGGGTGGTTTATGATTAAGGGTTTATATAAA
  TAAATGTTTCTAGTCTTCCGTGTGTCAAAATCCTCACCTCCTTCATAACCATCTCCCACA
  ATTAATTCTTGACTATATAAATTTATGGTTTGATAATATTATCAATTTGTAATCAATTGA
  GATTTCTTTAGTGCTTGCTTTTCTGTGACTCAACTGCCCAGACACCTCATTGTACTTGAA
  AACTGGAACAGCTTGGGAATGCCATGGGGTTTGATAATCTGCCAGGGACATGAAGAGGCT
  CAGCTTCCTGGACCATGACTTTGGCTCAGCTGATCCTGACATGGGAGAACAACCACATTT
  TTCTTTGTGTGTGCTTCTAGCAGCTGTTCGGGAGGACCTTGACCCAATAGTGTTCCCATG
  CTGTTTCTTGTGAAATGCTCTCGGCTATGTAGCAGCTTTTGATTCCCTGCATACCCTAGG
  CTGCTGCCCCTATCCTGTCCCTTGTTTATAACATTGAGAGGTTTTCTAGGGCACATACTG
  AGTGAGAGCAGTGTTGAGAAGTCGGGGAAAATGGTGACTACTTTTAGAGCAAGGCTGGGC
  ATCAGCACCTGTCCAGCTCTACTTGTGTGATGTTTCAGGAACTCAGCCCCTTTTTCTGCC
  TAGGATAAGGAGCTGAAAGATTAACTTGGATCTTCTAATGGTCCAAATCTTTTGGTCACA
  ATAAAGAGTCTCCAAATTAGAGACTGCATGTTAGTTCTGGATGGATTTGGTGGCCTGACA
  TGATACCCTGCCAGCTGTGAGGGGACCCCGTTTTTAAGATGCATGGCCAAGCTCTCTGCA
  AATGGAAATGCTTACACTGGGTGTTGGGGATGTTTGCTACCTCCTGCTATTTTTGTGGTT
  TTGGTTCTCCCACTATGGTAGGACCCCTGGCCAGCATTGTGGCTTGTCATGTCAGCCCCA
  TTGACTACCTTCTCATGCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002576 unedited
  CGCCAGGAATTTGTAACACACTCACTATAGGCGGCCGCGCAATTCGCACGAGCACCGCCG
  GGAGGAGGAGGAGGAGCCGAGAGGAGCTGAGCGAGCGCGGAAGTAGCTGCTGCTGGTGGT
  GACAATGTCAAATAACGGCCTAGACATTCAAGACAAACCCCCAGCCCCTCCGATGAGAAA
  TACCAGCACTATGATTGGAGCCGGCAGCAAAGATGCTGGAACCCTAAACCATGGTTCTAA
  ACCTCTGCCTCCAAACCCAGAGGAGAAGAAAAAGAAGGACCGATTTTACCGATCCATTTT
  ACCTGGAGATAAAACAAATAAAAAGAAAGAGAAAGAGCGGCCAGAGATTTCTCTCCCTTC
  AGATTTTGAACACACAATTCATGTCGGTTTTGATGCTGTCACAGGGGAGTTTACGGGAAT
  GCCAGAGCAGTGGGCCCGCTTGCTTCAGACATCAAATATCACTAAGTCGGAGCAGAAGAA
  AAACCCGCAAGCTGTTCTGGATGTGTTGGAGTTTTACAACTCGAAGAAGACATCCAACAG
  CCAGAAATACATGAGCTTTACAGATAAGTCAGCTGAGGATTACAATTCTTCTAATGCCTT
  GAATGTGAAGGCTGTGTCTGAGACTCCTGCAGTGCCACCAGTTTCAGAAGATGAGGATGA
  TGATGATGATGATGCTACCCCACCACCAGTGATTGCTCCACGCCCAGAGCACACAAAATC
  TGTATACACACGGTCTGTGATTGAACCACTTCCTGTCACTCCAACTCGGGACGTGGCTAC
  ATCTCCCATTTCACCTACTGAAAATAACACCACTCCACCAGATGCTTTGACCCGGAATAC
  TGAGAAGCAGAAGAAGAAGCCCTANATGTCTGACTGAGAGATCTTGGAGAATTACGAAGC
  TAGTGAGTGTGGGCGATCCTAAGAAGAATATACACGGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002576 unedited
  CCCCAATTAGTTTGNACCGTCGGCCGCATCCTACGAGCGAGTTTTTTTTTTTTTTTTTTT
  TTTTTATTTTCATCTCCTTTTATTGACAGAAATAGAAATTTGTGCTGCAGAGGCAGTAGT
  ACCTCAGAGCATGAGAAGGTAGTCAATGGGGCTGACATGACAAGCCACAATGCTGGCCAG
  GGGTCCTACCATAGTGGGAGAACCAAAACCACAAAAATAGCAGGAGGTAGCAAACATCCC
  CAACACCCAGTGTAAGCATTTCCATTTGCAGAGAGCTTGGCCATGCATCTTAAAAACGGG
  GTCCCCTCACAGCTGGCAGGGTATCATGTCAGGCCACCAAATCCATCCAGAACTAACATG
  CAGTCTCTAATTTGGAGACTCTTTATTGTGACCAAAAGATTTGGACCATTAGAAGAGCCA
  AGTTAATCTTTCAGCTCCTTATCCTAGGCAGAAAAAGGGGCTGAGTTCCTGAAACATCAC
  ACAAGTAGAGCTGGACAGGTGCTGATGCCCAGCCTTGCTCTAAAAGTAGTCACCATTTTC
  CCCGACTTCTCAACACTGCTCTCACTCAGTATGTGCCCTAGAAAACCTCTCAATGTTATA
  AACAAGGGACAGGATAGGGGCAGCAGCCTAGGGTATGCAGGGAATCAAAAGCTGCTACAT
  AGCCTAGAGCATTTCACAAGAAACAGCATGGGAACACTATTGGGTCAAGGTCCTTCCGAA
  CAGCTGCTAGAAGCACACACAAAGAACAATGTGGTTGTTCTCCCATGTCAGGATCAGCTG
  AGCCAAAGTCATGGTCCAGGAAGCTGAGCCTCTTCATGTCCCTGGCAGATATCAAACCCC
  ATGCCATTCCAAGCTGTTCCAGTTTTCAAGTCAATGAGTGTCTGGGCACATTGATCCAGA
  AC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002576
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002576.3, NP_002567.3
• RefSeq Size: 3264 bp
• RefSeq ORF: 1638 bp
• Locus ID: 5058
• Cytogenetics: 11q13.5-q14.1
• Domains: PBD, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: 'This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (2) lacks a coding exon in the 3' region, as compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.