BAD (NM_004322) Human Untagged Clone

CAT#: SC107955

BAD (untagged)-Human BCL2-associated agonist of cell death (BAD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BAD
• Synonyms: BBC2; BCL2L8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004322 edited
  ATGTTCCAGATCCCAGAGTTTGAGCCGAGTGAGCAGGAAGACTCCAGCTCTGCAGAGAGG
  GGCCTGGGCCCCAGCCCCGCAGGGGACGGGCCCTCAGGCTCCGGCAAGCATCATCGCCAG
  GCCCCAGGCCTCCTGTGGGACGCCAGTCACCAGCAGGAGCAGCCAACCAGCAGCAGCCAT
  CATGGAGGCGCTGGGGCTGTGGAGATCCGGAGTCGCCACAGCTCCTACCCCGCGGGGACG
  GAGGACGACGAAGGGATGGGGGAGGAGCCCAGCCCCTTTCGGGGCCGCTCGCGCTCGGCG
  CCCCCCAACCTCTGGGCAGCACAGCGCTATGGCCGCGAGCTCCGGAGGATGAGTGACGAG
  TTTGTGGACTCCTTTAAGAAGGGACTTCCTCGCCCGAAGAGCGCGGGCACAGCAACGCAG
  ATGCGGCAAAGCTCCAGCTGGACGCGAGTCTTCCAGTCCTGGTGGGATCGGAACTTGGGC
  AGGGGAAGCTCCGCCCCCTCCCAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004322 unedited
  NTTTCACGATTTGTATACGATTCATATAGGCGGCCGCGNAATTCGCACGAGGGGGAGGCG
  GCAGGCCCGGGTCAGGGGCCTCGAGATCGGGCTTGGGCCCAGAGCATGTTCCAGATCCCA
  GAGTTTGAGCCGAGTGAGCAGGAAGACTCCAGCTCTGCAGAGAGGGGCCTGGGCCCCAGC
  CCCGCAGGGGACGGGCCCTCAGGCTCCGGCAAGCATCATCGCCAGGCCCCAGGCCTCCTG
  TGGGACGCCAGTCACCAGCAGGAGCAGCCAACCAGCAGCAGCCATCATGGAGGCGCTGGG
  GCTGTGGAGATCCGGAGTCGCCACAGCTCCTACCCCGCGGGGACGGAGGACGACGAAGGG
  ATGGGGGAGGAGCCCAGCCCCTTTCGGGGCCGCTCGCGCTCGGCGCCCCCCAACCTCTGG
  GCAGCACAGCGCTATGGCCGCGAGCTCCGGAGGATGAGTGACGAGTTTGTGGACTCCTTT
  AAGAAGGGACTTCCTCGCCCGAAGAGCGCGGGCACAGCAACGCAGATGCGGCAAAGCTCC
  AGCTGGACGCGAGTCTTCCAGTCCTGGTGGGATCGGAACTTGGGCAGGGGAAGCTCCGCC
  CCCTCCCAGTGACCTTCGCTCCACATCCCGAAACTCCACCCGTTCCCACTGCCCTGGGCA
  GCCATCTTGAATATGGGCGGAAGTACTTCCCTCAGGCCTATGCAAAAAGAGGATCCGTGC
  TGTCTCCTTTGGAGGGAGGGCTGACCCAGATTCCCTTCCCGTGCGTGTGAAGCCCCCGGA
  AGCTTGGTCCCATCCGAAGTTATGGGTTTTCCGCCCCCAGCCGCCGGTAGTGGCTCCGTG
  GCCCCGCCCTCAGCTTCCGGCTTTCCCCCAGCGCCTGCCCTAATTGCGAGCCAGGTTTAC
  CGTTGTGTACCGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004322 unedited
  GCCCTCGATAGTCGAGTTTTTTTTTTTTTTTTTTTTGCACAAACGCGGTCTTTATTAACA
  TTTGGTAGTGAGCACGGCCCCCAGGGCATCGCGGGGGCTCGGGTCCCGGTGACGCAACGG
  TTAAACCTGGCTCGCGACTTAACGCAGGCGCCTGGGGGAAAGCCCGGAGCCTGAGGGCGG
  GGCCACGGAGCCACTTCCGGCGGCTGTGGGCGGAAAACCCAAAACTTCCGATGGGACCAA
  GCCTTCCGTGGCTTCACACGCACCGGAAGGGAATCTGGGTCAGCCCTCCCTCCAAAGGAG
  ACAGCACGGATCCTCTTTTTGCATAGGCCTGAGGGAAGTACTTCCGCCCATATTCAAGAT
  GGCTGCCCAGGGCAGTGGGAACGGGTGGAGTTTCGGGATGTGGAGCGAAGGTCACTGGGA
  GGGGGCGGAGCTTCCCCTGCCCAAGTTCCGATCCCACCAGGACTGGAAGACTCGCGTCCA
  GCTGGAGCTTTGCCGCATCTGCGTTGCTGTGCCCGCGCTCTTCGGGCGAGGAAGTNCCTT
  CTTAAAGGAGTCCACAAACTCGTCACTCATCCTCCGGAGCTCGCGGCCATAGCGCTGTGC
  TGCCCANAGTTGGGGGGCGCCNAGCGCGAGCGGCCCCNAAAGGGGCTGGGCTCCTCCCCA
  TCCCTTTGTCGTCCTCCGTCCCCGCGGGGTATGAGCTGTGGCGACTTCCGATCTCACAGC
  CCCAGCGCCTCATGATGGCTGCTGCTGGNTGGCTGCTCCTGCTGGTGACTGGCGTCCACA
  CGAAGCCTGNGGCCCTGCGATGATTCTTGCCGAAACCTGAGGCCCCTGTCCCTGTCGGGC
  TGGGGCCCAAGCCCCCTCTTGCAAGCTGAATCTTCTGCTACTCGCTCAACTTTGGGACTG
  AACATGTCTGGGCCAACCCGATTTGAGGCCTGAACGGNCTGCCCCTCCCTTTGCAGAATC
  GGGCCCCCTTAAGAGTGTTACAAATTGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004322
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004322.2, NP_004313.1
• RefSeq Size: 1127 bp
• RefSeq ORF: 507 bp
• Locus ID: 572
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Insulin signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, VEGF signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Dec 2019]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.