RAP1GDS1 (NM_021159) Human Untagged Clone

CAT#: SC108060

RAP1GDS1 (untagged)-Human RAP1, GTP-GDP dissociation stimulator 1 (RAP1GDS1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAP1GDS1
• Synonyms: GDS1; SmgGDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021159, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGATAATCTCAGTGATACCTTGAAGAAGCTGAAGATAACAGCTGTTGACAAGACTGAGGATAGTT
  TAGAAGGATGCTTGGATTGTCTGCTTCAAGCCCTGGCTCAAAATAATACGGAAACAAGTGAAAAAATCCA
  AGCAAGTGGAATACTTCAGCTGTTTGCAAGTCTGTTGACTCCACAGTCTTCCTGCAAAGCCAAAGTAGCT
  AACATCATAGCAGAAGTAGCCAAAAATGAGTTTATGCGAATTCCATGTGTGGATGCTGGATTGATTTCAC
  CACTGGTGCAGCTGCTAAATAGCAAAGACCAGGAAGTGCTGCTTCAAACGGGCAGGGCTCTAGGAAACAT
  ATGTTACGATAGCCATGAGGGCAGAAGTGCAGTTGACCAAGCAGGTGGTGCACAGATTGTAATTGACCAT
  TTAAGGTCACTGTGCAGTATAACAGATCCCGCCAATGAGAAGCTCTTGACTGTCTTTTGTGGCATGCTGA
  TGAACTATAGCAATGAGAATGATTCGCTTCAAGCTCAGCTTATCAATATGGGTGTTATTCCTACCTTAGT
  GAAATTACTGGGCATCCACTGCCAAAATGCAGCTCTTACAGAAATGTGTCTTGTTGCATTTGGTAATTTA
  GCAGAACTTGAGTCAAGTAAAGAACAGTTTGCCAGTACAAACATTGCTGAAGAGCTAGTAAAACTCTTCA
  AGAAACAAATAGAACATGATAAGAGAGAAATGATTTTTGAAGTTCTTGCTCCATTGGCAGAAAATGATGC
  TATTAAACTACAGCTGGTTGAAGCAGGCCTAGTAGAGTGTCTACTAGAGATTGTTCAGCAAAAAGTGGAT
  AGTGACAAAGAAGATGATATTACTGAGCTCAAAACTGGTTCAGATCTCATGGTTTTATTACTTCTTGGAG
  ATGAATCCATGCAGAAGTTATTTGAAGGAGGAAAAGGTAGTGTATTTCAAAGGGTACTCTCTTGGATCCC
  ATCAAATAACCACCAGCTACAGCTTGCTGGAGCATTGGCAATTGCAAATTTTGCCAGAAATGATGCAAAT
  TGTATTCATATGGTAGACAATGGGATTGTAGAAAAACTTATGGATTTACTGGACAGACATGTAGAAGATG
  GAAATGTAACAGTACAGCATGCAGCACTAAGTGCCCTCAGAAACCTGGCCATTCCAGTTATAAATAAAGC
  AAAGATGTTATCAGCTGGGGTCACAGAGGCAGTTTTGAAATTTCTTAAATCTGAAATGCCTCCTGTTCAG
  TTCAAACTTCTGGGAACATTAAGAATGTTAATAGATGCACAAGAAGCTGCTGAACAATTGGGAAAGAATG
  TTAAGTTAGTGGAGCGTTTGGTGGAATGGTGTGAAGCCAAAGATCATGCTGGTGTGATGGGGGAGTCAAA
  CAGACTGCTGTCTGCCCTTATACGACACAGTAAATCAAAAGATGTAATTAAAACCATTGTGCAGAGTGGT
  GGCATCAAGCATCTAGTTACCATGGCAACTAGTGAACATGTAATAATGCAGAATGAAGCTCTTGTTGCTT
  TGGCATTAATAGCAGCTTTAGAATTGGGCACTGCTGAGAAAGATCTAGAAAGTGCTAAACTTGTACAGAT
  TTTACATAGACTGCTAGCAGATGAGAGAAGTGCTCCTGAAATCAAATATAATTCCATGGTCCTGATATGT
  GCTCTTATGGGATCTGAATGTCTACACAAGGAAGTACAGGATTTGGCTTTTCTAGATGTCGTATCCAAAC
  TTCGCAGTCATGAGAACAAAAGTGTTGCCCAGCAGGCCTCTCTCACAGAGCAGAGACTTACTGTGGAAAG
  CTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021159 unedited
  AGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAGAGACGG
  AGGTAGAGGGAGGACACAGAGCCGCGCCGCCCGCACCACAGACCTTCGCCTCGCCCCGCC
  GGTTCCTCACCCTCGGGGAGCAACATGGATAATCTCAGTGATACCTTGAAGAAGCTGAAG
  ATAACAGCTGTTGACAAGACTGAGGATAGTTTAGAAGGATGCTTGGATTGTCTGCTTCAA
  GCCCTGGCTCAAAATAATACGGAAACAAGTGAAAAAATCCAAGCAAGTGGAATACTTCAG
  CTGTTTGCAAGTCTGTTGACTCCACAGTCTTCCTGCAAAGCCAAAGTAGCTAACATCATA
  GCAGAAGTAGCCAAAAATGAGTTTATGCGAATTCCATGTGTGGATGCTGGATTGATTTCA
  CCACTGGTGCAGCTGCTAAATAGCAAAGACCAGGAAGTGCTGCTTCAAACGGGCAGGGCT
  CTAGGAAACATATGTTACGATAGCCATGAGGGCAGAAGTGCAGTTGACCAAGCAGGTGGT
  GCACAGATTGTAATTGACCATTTAAGGTCACTGTGCAGTATAACAGATCCCGCCAATGAG
  AAGCTCTTGACTGTCTTTTGTGGCATGCTGATGAACTATAGCAATGAGAATGATTCGCTT
  CAAGCTCAGCTTATCAATATGGGTGTTATTCCTACCTTAGTGAAATTACTGGGCATCCAC
  TGCCAAAATGCAGCTCTTACAGAATGTGTCTTGTTGCATTTGGTAATTTAGCAGAACTTG
  AGTCAAGTAAAGAACAGTTTGCCAGTACAAACATTGCTGAAGAGCTAGTAAAACTCTTCA
  GAAACAATAGACATGATAGAGAGAATGATTTTTGAAGTCTTGCTCATTGCAGAATGAGCT
  ATAACTACACTGTTGAGCAGCTATTAAATGCTCTANAATGTCGCAAGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021159 unedited
  GGTGGCNGACAAAGGCCAATTTTTANNTAACTNCTAGGGTACCATGGGATTTGACATCAG
  TAGGAAAGACTATTGAATAAGCAGCCATACAACTTTTTTGGTCACTGTGCTTCCCAAGCA
  CCAAAAAATGGAGCTATTTTTCCAATTCAGTTTCATCTTTAATAAGCTAGACAGATCCTG
  CTGCATTCTTTGTTTCTAGTTTACCAGTACATCATTATGTAGTTTACTGCATGTGTGGAG
  CAAGTCATTATGTAGTACCAAGTTTACATCATCATAGGACTACAACATGCACATTCTACT
  TAATGTATCTCAAGCCACAGGAATACAAGATAACGGTTATGGGTGGAGACTTGCCTTTGA
  CAAAGTCATGATCACACTAACTATAGGTTTAGAAATCTTATTAGGGAGGTACCTACAGCG
  GGTCTTNAATTGGTATTATAACCTNACATGCGTTGCCACGTGGTATGGTTTTTTAATGAC
  ACCGCCAGANCCCTTTGCTCGTTTGACAGTAGGGGACATTTTTTAGGGCTTTTGCTTTTT
  TTNCGCCACTATCCTTTTCTTTTACAGATCCCCTTCGCNTTGTTNAAAGGCCTGCTGAGC
  ACCATTATTGCCTTCCCAGCTGTTGTCCTTTTTATCCCGTCTTTCCCCGNCCTTTTCTTT
  TTTTTATCATTGTGCCCACCCTTCCTATTCTAATGACNATTATTACTGCAACCTCCTTCG
  ATTTGTTCTTCGAGACCCTCTCCTCTATACATCTANCGGCTCATTCTCTTATTCCCACNC
  ACCGACCTCAATACCGCCGCTCAACGCCCCTACAACCAANCNCGACGCGCCNCTATTTTG
  TGAGACCGTATTTCTTCTTTTCTTCAGCATCTCCCCCCGGGAGATTCACCATAAATATAT
  NATCGGCACGTCGACGATTCTTACACCTATGTGCGACCGCGCCCNNCTTTCGATTATTAC
  TACGCTAGTTTTATACCACACCATTTCGACGCACCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021159
• Insert Size: 3050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021159.3, NP_066982.2
• RefSeq Size: 2487 bp
• RefSeq ORF: 1824 bp
• Locus ID: 5910
• Cytogenetics: 4q23
• Domains: Armadillo_seg
• Gene Summary: 'The smg GDP dissociation stimulator (smgGDS) protein is a stimulatory GDP/GTP exchange protein with GTPase activity (Riess et al., 1993 [PubMed 8262526]).[supplied by OMIM, Feb 2010]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.