CYLD (NM_015247) Human Untagged Clone

CAT#: SC108180

CYLD (untagged)-Human cylindromatosis (turban tumor syndrome) (CYLD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYLD
• Synonyms: BRSS; CDMT; CYLD1; CYLDI; EAC; MFT; MFT1; SBS; TEM; USPL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108180 sequence for NM_015247 edited (data generated by NextGen Sequencing)
  ATGAGTTCAGGCTTATGGAGCCAAGAAAAAGTCACTTCACCCTACTGGGAAGAGCGGATT
  TTTTACTTGCTTCTTCAAGAATGCAGCGTTACAGACAAACAAACACAAAAGCTCCTTAAA
  GTACCGAAGGGAAGTATAGGACAGTATATTCAAGATCGTTCTGTGGGGCATTCAAGGATT
  CCTTCTGCAAAAGGCAAGAAAAATCAGATTGGATTAAAAATTCTAGAGCAACCTCATGCA
  GTTCTCTTTGTTGATGAAAAGGATGTTGTAGAGATAAATGAAAAGTTCACAGAGTTACTT
  TTGGCAATTACCAATTGTGAGGAGAGGTTCAGCCTGTTTAAAAACAGAAACAGACTAAGT
  AAAGGCCTCCAAATAGACGTGGGCTGTCCTGTGAAAGTACAGCTGAGATCTGGGGAAGAA
  AAATTTCCTGGAGTTGTACGCTTCAGAGGACCCCTGTTAGCAGAGAGGACAGTCTCCGGA
  ATATTCTTTGGAGTTGAATTGCTGGAAGAAGGTCGTGGTCAAGGTTTCACTGACGGGGTG
  TACCAAGGGAAACAGCTTTTTCAGTGTGATGAAGATTGTGGCGTGTTTGTTGCATTGGAC
  AAGCTAGAACTCATAGAAGATGATGACACTGCATTGGAAAGTGATTACGCAGGTCCTGGG
  GACACAATGCAGGTCGAACTTCCTCCTTTGGAAATAAACTCCAGAGTTTCTTTGAAGGTT
  GGAGAAACAATAGAATCTGGAACAGTTATATTCTGTGATGTTTTGCCAGGAAAAGAAAGC
  TTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGGGATGGAAGATTT
  GATGGAGTGCAGCTTTGTAGTTTTGCGTGTGTTGAAAGTACAATTCTATTGCACATCAAT
  GATATCATCCCANNNNNNNNNGAGAGTGTGACGCAGGAAAGGAGGCCTCCCAAACTTGCC
  TTTATGTCAAGAGGTGTTGGGGACAAAGGTTCATCCAGTCATAATAAACCAAAGGCTACA
  GGATCTACCTCAGACCCTGGAAATAGAAACAGATCTGAATTATTTTATACCTTAAATGGG
  TCTTCTGTTGACTCACAACCACAATCCAAATCAAAAAATACATGGTACATTGATGAAGTT
  GCAGAAGACCCTGCAAAATCTCTTACAGAGATATCTACAGACTTTGACCGTTCTTCACCA
  CCACTCCAGCCTCCTCCTGTGAACTCACTGACCACCGAGAACAGATTCCACTCTTTACCA
  TTCAGTCTCACCAAGATGCCCAATACCAATGGAAGTATTGGCCACAGTCCACTTTCTCTG
  TCAGCCCAGTCTGTAATGGAAGAGCTAAACACTGCACCCGTCCAAGAGAGTCCACCCTTG
  GCCATGCCTCCTGGGAACTCACATGGTCTAGAAGTGGGCTCATTGGCTGAAGTTAAGGAG
  AACCCTCCTTTCTATGGGGTAATCCGTTGGATCGGTCAGCCACCAGGACTGAATGAAGTG
  CTCGCTGGACTGGAACTGGAAGATGAGTGTGCAGGCTGTACGGATGGAACCTTCAGAGGC
  ACTCGGTATTTCACCTGTGCCCTGAAGAAGGCGCTGTTTGTGAAACTGAAGAGCTGCAGG
  CCTGACTCTAGGTTTGCATCATTGCAGCCGGTTTCCAATCAGATTGAGCGCTGTAACTCT
  TTAGCATTTGGAGGCTACTTAAGTGAAGTAGTAGAAGAAAATACTCCACCAAAAATGGAA
  AAAGAAGGCTTGGAGATAATGATTGGGAAGAAGAAAGGCATCCAGGGTCATTACAATTCT
  TGTTACTTAGACTCAACCTTATTCTGCTTATTTGCTTTTAGTTCTGTTCTGGACACTGTG
  TTACTTAGACCCAAAGAAAAGAACGATGTAGAATATTATAGTGAAACCCAAGAGCTACTG
  AGGACAGAAATTGTTAATCCTCTGAGAATATATGGATATGTGTGTGCCACAAAAATTATG
  AAACTGAGGAAAATACTTGAAAAGGTGGAGGCTGCATCAGGATTTACCTCTGAAGAAAAA
  GATCCTGAGGAATTCTTGAATATTCTGTTTCATCATATTTTAAGGGTAGAACCTTTGCTA
  AAAATAAGATCAGCAGGTCAAAAGGTACAAGATTGTTACTTCTATCAAATTTTTATGGAA
  AAAAATGAGAAAGTTGGCGTTCCCACAATTCAGCAGTTGTTAGAATGGTCTTTTATCAAC
  AGTAACCTGAAATTTGCAGAGGCACCATCATGTCTGATTATTCAGATGCCTCGATTTGGA
  AAAGACTTTAAACTATTTAAAAAAATTTTTCCTTCTCTGGAATTAAATATAACAGATTTA
  CTTGAAGACACTCCCAGACAGTGCCGGATATGTGGAGGGCTTGCAATGTATGAGTGTAGA
  GAATGCTACGACGATCCGGACATCTCAGCTGGAAAAATCAAGCAGTTTTGTAAAACCTGC
  AACACTCAAGTCCACCTTCATCCGAAGAGGCTGAATCATAAATATAACCCAGTGTCACTT
  CCCAAAGACTTACCCGACTGGGACTGGAGACACGGCTGCATCCCTTGCCAGAATATGGAG
  TTATTTGCTGTTCTCTGCATAGAAACAAGCCACTATGTTGCTTTTGTGAAGTATGGGAAG
  GACGATTCTGCCTGGCTCTTCTTTGACAGCATGGCCGATCGGGATGGTGGTCAGAATGGC
  TTCAACATTCCTCAAGTCACCCCATGCCCAGAAGTAGGAGAGTACTTGAAGATGTCTCTG
  GAAGACCTGCATTCCTTGGACTCCAGGAGAATCCAAGGCTGTGCACGAAGACTGCTTTGT
  GATGCATATATGTGCATGTACCAGAGTCCAACAATGAGTTTGTACAAATAA
  
  Clone variation with respect to NM_015247.2
  913 g=>n;914 c=>n;915 t=>n;916 t=>n;917 t=>n;918 a=>n;919 t=>n;920 c=>n;921 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015247 unedited
  CTGGATTTGTAACGACTTATATAGGCGGCACAGCAATTCGCACGAGGTTTGGCTGCGCGG
  GGGCCGCGCGTCGGAGTTTCCCCCTTTCTAGGGTGAGGATGGTTCTACACAGCCACCCGG
  AGTTCCTTAGTTGAAAGGTGCGCCCTGCTGTGACAGCATGGACACCACGTTGCTGAAAAC
  ATGCTTTGGGACTGCCACTGAATTTATCTTTTGCGGTTTTATGACAAAGTTATTAGTAGT
  TTCCCTTTTTTGAATTAGTATTTTGAAGTTAATATCACAATGAGTTCAGGCTTATGGAGC
  CAAGAAAAAGTCACTTCACCCTACTGGGAAGAGCGGATTTTTTACTTGCTTCTTCAAGAA
  TGCAGCGTTACAGACAAACAAACACAAAAGCTCCTTAAAGTACCGAAGGGAAGTATAGGA
  CAGTATATTCAAGATCGTTCTGTGGGGCATTCAAGGATTCCTTCTGCAAAAGGCAAGAAA
  AATCAGATTGGATTAAAAATTCTAGAGCAACCTCATGCAGTTCTCTTTGTTGATGAAAAG
  GATGTTGTAGAGATAAATGAAAAGTTCACAGAGTTACTTTTGGCAATTACCAATTGTGAG
  GAGAGGTTCAGCCTGTTTAANAACAGANACAGACTAAGTAAAGGCCTCCAAATAGACGTG
  GGCTGTCCTGTGAAAGTACAGCTGAGATCTGGNGAAGAAAAATTTCCTGGAGTTGTACGC
  TTCAGAAGACCCCTGGTAGCAGAGAGGACAGTCNTCGNAATATTTCTTGGNAGTGAATTG
  CTGGAAGAANGTCGTGGTCAAGGTTTCACTGACGGGGTGTACCAAGGNAAACAGCTTTTC
  AGTGTGATGAAAATTGTGCGTGTTTGTTGCATTGGACAAGCTAGACTCTAANAAGAGATG
  ACACTGCATTGGAAGTGATTACCCAGTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015247 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTGGTCCATAAAGAA
  GAACATTTATTTACATTCTCAATGGACTAAAAGAGGCTTTAAACTTCCACAATTAAAATT
  GTATGCTTCCAAAAATATCATATGATCAATGCACTTAACTTAAAGCTTCAGGTATTAATA
  ACTTCATTTAGACTTCTTAAAAAACCAACACAAACATACTTTCTAGAGTGCAAAATGCTT
  CTTATTAAATACTTCAGGGACACAAAAGCAATTTGTTTTTAAACAGAGGCATCCTTTTCT
  GAAGGATCATCACCACAAAGACATCCATTGCCGGCAATGGACGTGAACAGAACTGCCAGC
  TCGAATAAGATGCAACGTTAGGACTCTGCCTTCAGTTTCTTTGCCTTTCCCGATGACCCC
  AGTTATTTGTACAAACTCATTGTTGGACTCTGGTACATGCACATATATGCATCACAAAGC
  AGTCTTCGTGCACAGCCTTGGATTCTCCTGGAGTCCAAGGAATGCAGGTCTTCCAGAGAC
  ATCTTCAAGTACTCTCCTACTTCTGGGCATGGGGTGACTTGAGGAATGTTGAAGCCATTC
  TGACCACCATCCCGATCGGCCATGCTGTCAAAGAAGAGCCAGGCAGAATCGTCCTTCCCA
  TACTTCACANAAGCAACATAGTGGCTTGTTTCTATGCAGAGAACAGCANATAACTCCATA
  TTCTGGCAAGGGATGCAGCCGTGTCTTCAGTCCCAGTCGGGTAAGTCTTTGGGAGTGACC
  CTGGTTATATTTATGATTAGCCTCTTCGATGAAGGTGACTTGAGTGTTGCAGGTTTACAA
  AACTGCTTGATTTTCCAGCTGAGATGCCGGATCGTCNAACATTCTCTACCTCATACATTG
  CAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015247
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015247.1, NP_056062.1
• RefSeq Size: 5371 bp
• RefSeq ORF: 2871 bp
• Locus ID: 1540
• Cytogenetics: 16q12.1
• Domains: CAP_GLY, UCH
• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway
• Gene Summary: 'This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).