PPAR gamma (PPARG) (NM_138711) Human Untagged Clone

CAT#: SC108192

PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPARG
• Synonyms: CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARgamma
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138711 edited
  CAGAAGCCTGCATTTCTGCATTCTGCTTAATTCCCTTTCCTTAGATTTGAAAGAAGCCAA
  CACTAAACCACAAATATACAACAAGGCCATTTTCTCAAACGAGAGTCAGCCTTTAACGAA
  ATGACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTTGGGATCAGCTCCGTG
  GATCTCTCCGTAATGGAAGACCACTCCCACTCCTTTGATATCAAGCCCTTCACTACTGTT
  GACTTCTCCAGCATTTCTACTCCACATTACGAAGACATTCCATTCACAAGAACAGATCCA
  GTGGTTGCAGATTACAAGTATGACCTGAAACTTCAAGAGTACCAAAGTGCAATCAAAGTG
  GAGCCTGCATCTCCACCTTATTATTCTGAGAAGACTCAGCTCTACAATAAGCCTCATGAA
  GAGCCTTCCAACTCCCTCATGGCAATTGAATGTCGTGTCTGTGGAGATAAAGCTTCTGGA
  TTTCACTATGGAGTTCATGCTTGTGAAGGATGCAAGGGTTTCTTCCGGAGAACAATCAGA
  TTGAAGCTTATCTATGACAGATGTGATCTTAACTGTCGGATCCACAAAAAAAGTAGAAAT
  AAATGTCAGTACTGTCGGTTTCAGAAATGCCTTGCAGTGGGGATGTCTCATAATGCCATC
  AGGTTTGGGCGGATGCCACAGGCCGAGAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGAT
  ATCGACCAGCTGAATCCAGAGTCCGCTGACCTCCGGGCCCTGGCAAAACATTTGTATGAC
  TCATACATAAAGTCCTTCCCGCTGACCAAAGCAAAGGCGAGGGCGATCTTGACAGGAAAG
  ACAACAGACAAATCACCATTCGTTATCTATGACATGAATTCCTTAATGATGGGAGAAGAT
  AAAATCAAGTTCAAACACATCACCCCCCTGCAGGAGCAGAGCAAAGAGGTGGCCATCCGC
  ATCTTTCAGGGCTGCCAGTTTCGCTCCGTGGAGGCTGTGCAGGAGATCACAGAGTATGCC
  AAAAGCATTCCTGGTTTTGTAAATCTTGACTTGAACGACCAAGTAACTCTCCTCAAATAT
  GGAGTCCACGAGATCATTTACACAATGCTGGCCTCCTTGATGAATAAAGATGGGGTTCTC
  ATATCCGAGGGCCAAGGCTTCATGACAAGGGAGTTTCTAAAGAGCCTGCGAAAGCCTTTT
  GGTGACTTTATGGAGCCCAAGTTTGAGTTTGCTGTGAAGTTCAATGCACTGGAATTAGAT
  GACAGCGACTTGGCAATATTTATTGCTGTCATTATTCTCAGTGGAGACCGCCCAGGTTTG
  CTGAATGTGAAGCCCATTGAAGACATTCAAGACAACCTGCTACAAGCCCTGGAGCTCCAG
  CTGAAGCTGAACCACCCTGAGTCCTCACAGCTGTTTGCCAAGCTGCTCCAGAAAATGACA
  GACCTCAGACAGATTGTCACGGAACACGTGCAGCTACTGCAGGTGATCAAGAAGACGGAG
  ACAGACATGAGTCTTCACCCGCTCCTGCAGGAGATCTACAAGGACTTGTACTAGCAGAGA
  GTCCTGAGCCACTGCCAACATTTCCCTTCTTCCAGTTGCACTATTCTGAGGGAAAATCTG
  ACACCTAAGAAATTTACTGTGAAAAAGCATTTTAAAAAGAAAAGGTTTTAGAATATGATC
  TATTTTATGCATATTGTTTATAAAGACACATTTACAATTTACTTTTAATATTAAAAATTA
  CCATATTATGAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138711 unedited
  CCATATGTATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGCAGAAGCCTGCAT
  TTCTGCATTCTGCTTAATTCCCTTTCCTTAGATTTGAAAGAAGCCAACACTAAACCACAA
  ATATACAACAAGGCCATTTTCTCAAACGAGAGTCAGCCTTTAACGAAATGACCATGGTTG
  ACACAGAGATGCCATTCTGGCCCACCAACTTTGGGATCAGCTCCGTGGATCTCTCCGTAA
  TGGAAGACCACTCCCACTCCTTTGATATCAAGCCCTTCACTACTGTTGACTTCTCCAGCA
  TTTCTACTCCACATTACGAAGACATTCCATTCACAAGAACAGATCCAGTGGTTGCAGATT
  ACAAGTATGACCTGAAACTTCAAGAGTACCAAAGTGCAATCAAAGTGGAGCCTGCATCTC
  CACCTTATTATTCTGAGAAGACTCAGCTCTACAATAAGCCTCATGAAGAGCCTTCCAACT
  CCCTCATGGCAATTGAATGTCGTGTCTGTGGAGATAAAGCTTCTGGATTTCACTATGGAG
  TTCATGCTTGTGAAGGATGCAAGGGTTTCTTCCGGAGAACAATCAGATTGAAGCTTATCT
  ATGACAGATGTGATCTTAACTGTCGGATCCACAAAAAAAGTAGAAATAAATGTCAGTACT
  GTCGGTTTCAGAAATGCCTTGCAGTGNGGATGTCTCATAATGCCATCAGGTTTGGGCGGA
  TGCCACAGGCCGAGAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGA
  ATCCAGAGTCCGCTGACCTCCGGGCCCTGGCAAACATTNGTATGACTCATACATAAAGTC
  CTTCCCGCTGACCAAAGCANAGGCGAGGNCGATCTTGACAGGAANNGACACAGACAATCA
  CCATTCGTTATCTATGACATGAANNTCCTTATGATGGNAGAAGATANAATCAAGTTCAAC
  ACTCACCCCCCTGCAGGAGCAGACCAAGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138711 unedited
  ATCTNTGNNACCGCGGCCGCAATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTCATAAT
  ATGGTAATTTTTAATATTAAAAGTAAATTGTAAATGTGTCTTTATAAACAATATGCATAA
  AATAGATCATATTCTAAAACCTTTTCTTTTTAAAATGCTTTTTCACAGTAAATTTCTTAG
  GTGTCAGATTTTCCCTCAGAATAGTGCAACTGGAAGAAGGGAAATGTTGGCAGTGGCTCA
  GGACTCTCTGCTAGTACAAGTCCTTGTAGATCTCCTGCAGGAGCGGGTGAAGACTCATGT
  CTGTCTCCGTCTTCTTGATCACCTGCAGTAGCTGCACGTGTTCCGTGACAATCTGTCTGA
  GGTCTGTCATTTTCTGGAGCAGCTTGGCAAACAGCTGTGAGGACTCAGGGTGGTTCAGCT
  TCAGCTGGAGCTCCAGGGCTTGTAGCAGGTTGTCTTGAATGTCTTCAATGGGCTTCACAT
  TCAGCAAACCTGGGCGGTCTCCACTGAGAATAATGACAGCAATAAATATTGCCAAGTCGC
  TGTCATCTAATTCCAGTGCATTGAACTTCACAGCAAACTCAAACTTGGGCTNCATAAAGT
  CACCAAAAGGCTTTCGCAGGCTCTTTAGAAACTCCCTTGTCATGAAGCCTTGGCCCTCGG
  ATATGAGAACCCCATCTTTATTCATCAAGGAGGCCAGCATTGTGTAAATGATCCCGTGGA
  CTCCATATTTGAGGAGAGGTACTTGGGCCGTTCAAGTCAGGATTACAAAACCAGGATGCT
  TTTNGCATACTCTGTGAACTCCTGCCCAGCCTCCCGGAGCGAAACTGCAGCCCTGAAGAT
  GCGGATGGCCACCTTTTGCTCTGCTCTGCAGGGGGTGATGTGTTNGAACCTTGATTTTCC
  TCTCCATCCTTAGGGAATCATGGCAAGATACCAAGGGGATTTGCCTGGTGGCTTTCTGGC
  AAGACCGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138711
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138711.2, NP_619725.1
• RefSeq Size: 1920 bp
• RefSeq ORF: 1919 bp
• Locus ID: 5468
• Cytogenetics: 3p25.2
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Huntington's disease, Pathways in cancer, PPAR signaling pathway, Thyroid cancer
• Gene Summary: 'This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region compared to variant 2. Variants 1, 3, 4, 6, and 7 all encode the same isoform (1), which has a shorter, distinct N-terminus compared to isoform 2.