ITCH (NM_031483) Human Untagged Clone

CAT#: SC108233

ITCH (untagged)-Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ITCH
• Synonyms: ADMFD; AIF4; AIP4; NAPP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031483, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTG
  TCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGT
  AGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACA
  GTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTT
  TGTTGGGAACTGCTGCATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
  TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAGACTTGTCAATTTGTCTTGAT
  GGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATG
  ATGATGGCTCCAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTGAAGATGCAGG
  AGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCATCACTCTCAAATGGTGGTTTTAAACCTTCT
  AGACCTCCAAGACCTTCACGACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT
  CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACAAATACAAATACAAATACATC
  TGAAGGAGCAACATCTGGATTAATAATTCCTCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAAT
  CCTGTAACTCAAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGAGTTTACTATG
  TAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGAACCTCTACCTCCTGGCTGGGAACGGCGGGT
  TGACAACATGGGACGTATTTATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG
  GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCAAGGAGCAATGCAGCAGTTTA
  ACCAGAGATTCATTTATGGGAATCAAGATTTATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGG
  TCCATTGCCACCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAACCACAACACA
  CGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAATTAAATGAAAAGCCCTTACCTGAAGGTTGGG
  AAATGAGATTCACAGTGGATGGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA
  TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATGTTCGGGACTTCAAAGCAAAG
  GTTCAGTATTTCCGGTTCTGGTGTCAGCAACTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAA
  AAACATTGTTTGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAAGACGTTTGTG
  GGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCA
  CATGAAGTGTTGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA
  ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATTGGCAGATTTATTGCCATGGC
  TCTGTTCCATGGGAAATTCATAGACACGGGTTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCA
  GTTGGACTCAAGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTTAAGGAAAACA
  ATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGACAAAGAAATTCTAGGTGAAATTAAGAGTCA
  TGATCTGAAACCTAATGGTGGCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA
  GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTTTGAAGGCTTTAATGAAATTC
  TTCCCCAGCAATATTTGCAATACTTTGATGCAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGAT
  TGATTTGAATGACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACAAATCATGTGG
  TTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAATGAGACTTCTGCAGTTTGTTACTGGAACCT
  GCCGATTGCCAGTAGGAGGATTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA
  AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGCCTGGACCTGCCACCATACAAG
  AGCTATGAGCAACTGAAGGAAAAGCTGTTGTTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031483 unedited
  GGGCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGATGCATTTCA
  CAGTGGCCTTGTGGAGACAACGCCTTAACCCAAGGAAGTGACTCAAACTGTGAGAACTTC
  AGGTTTTCCAACCTATTGGTGGTATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTA
  GCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATA
  AGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGA
  AGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCG
  TTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATG
  TTTTGTTGGGAACTGCTGCATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAAC
  TTGAAGAAGTAGTTGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAG
  GAGACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTG
  AAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTCCAGATCCAAGGATGAAA
  CAAGAGTGAGCACAAATGGATCAGATGACCCTGAAGATGCAAGAGCTGGTGAAAATAGGA
  GAGTCAGTGGGAATAATTCTCCATCACTCTCAAATGGTGGTTTTAAACCTCTAGACCTCA
  AGACCTTCACGACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGGTCACCA
  TCCTGCCACTTTGAAAGTGAGGGTCTAGTACAAGCTCTCCTGCCCCGACAAAACCAATAC
  AATACATCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031483 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTTATCCCCCACCACGCTGCCC
  AAAAAAAAGAACAGGGTCTCCCTATGTTGCCCAGGCTGGTCTTTAACTCCTGGGCTAAAG
  GGATCCTCCTGCCTCGGCCTCCCAGAGTGCTAGGATTACAGGTATGAGCCACCATGCCCG
  GCCCTAGGTTAACTTCTTAAAACAATGAAGGAATCACAGTGTTAACATTGTTTGTAAAGT
  GTAGTATTTGTCAGGCAACTGAAACAATCAGACTTCCAAATGAAGTCATTTGCAGGCTGT
  GACCAACTTTTCCCTGAATTCAAATTGACAAAACTACTTTGTAGAGATGCAAGAGTCTTG
  CCTCACTTCCAGGAATGATGTATGGATTCATGCGCCAGAAAGGAAAAACAATAGTCAAGT
  CAGACTAAAATGAGAGGGAGAACACAAGCCCGAATTTTGCTCACATCCNCAGAAGCAGGA
  GTAAGCTTGCACTTTTAACAAATGCCTCTTACCAAGAGGCTTAATAGGAAAATCTTGTAT
  ATTGCAGTTACCAAAGGACTTCCCTGCATGTCTGCAGAATGTCTAANAGAAAAAACACTA
  CTCCCAAGTGAGACTTTTGACACACATAANATATTGTGTTAAAGGAATAACTAGTGGAAT
  AGACAAGGGCAATTCATTGTGTGTAAAATCTATGTTAGCAGGTTCTGACTAGTCATTGCA
  GACCTGATTCTGGAACGCTGAGTGAAATCACTGGAGATAATCTAATGAGACATTATTTAC
  TTCACTCAGATACTCTCTAAGACCCATTGTCATTTACAGATGTGCCACAGCANAAAAGAC
  AAACATGCAATTAGTCCTGCCATTCATGTGCCAGTTTAAAGTACTCTGCCAATCCTCTGT
  TTTCTATGCAACAAAGCTTNCTAGTGCTAAACTCTGGTGGGGCAGTCAGCGATAAACGGT
  GACTCTGGACCATTTCTTCCACTTTCAGCAATTCTTGGCATGCTCCAGAAAACAACTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031483
• ORF Size: 2589 bp
• Insert Size: 3910
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_031483.3, NP_113671.3
• RefSeq Size: 6357
• RefSeq ORF: 2589
• Locus ID: 83737
• Domains: C2, HECT, WW
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Both variants 2 and 5 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.