FOXA1 (NM_004496) Human Untagged Clone

CAT#: SC108256

FOXA1 (untagged)-Human forkhead box A1 (FOXA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FOXA1
• Synonyms: HNF3A; TCF3A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  NCBI ORF sequence for NM_004496, the custom clone sequence may differ by one or more nucleotides
  GAATTCGGCACGAGGGTGGCGTTGGGCCCGCGCGGGCGCTCGGGTGACTGCAGCTGCTCA
  GCTCCCCTCCCCCGCCCCGCGCCGCGCGGCCGCCCGTCGCTTCGCACAGGGCTGGATGGT
  TGTATTGGGCAGGGTGGCTCCAGGATGTTAGGAACTGTGAAGATGGAAGGGCATGAAACC
  AGCGACTGGAACAGCTACTACGCAGACACGCAGGAGGCCTACTCCTCCGTCCCGGTCAGC
  AACATGAACTCAGGCCTGGGCTCCATGAACTCCATGAACACCTACATGACCATGAACACC
  ATGACTACGAGCGGCAACATGACCCCGGCGTCCTTCAACATGTCCTATGCCAACCCGGGC
  CTAGGGGCCGGCCTGAGTCCCGGCGCAGTAACCGGCATGCCGGGGGGCTCGGCGGGCGCC
  ATGAACAGCATGACTGCGGCCGGCGTGACGGCCATGGGTACGGCGCTGAGCCCGAGCGGC
  ATGGGCGCCATGGGTGCGCAGCAGGCGGCCTCCATGAATGGCCTGGGCCCCTACGCGGCC
  GCCATGAACCCGTGCATGAGCCCCATGGCGTACGCGCCGTCCAACCTGGGCCGCAGCCGC
  GCGGGCGGCGGCGGCGACGCCAAGACGTTCAAGCGCAGCTACCCGCACGCCAAGCCGCCC
  TACTCGTACATCTCGCTCATCACCATGGCCATCCAGCAGGCGCCCAGCAAGATGCTCACG
  CTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTATTACCGGCAGAACCAGCAG
  CGCTGGCAGAACTCCATCCGCCACTCGCTGTCCTTCAATGACTGCTTCGTCAAGGTGGCA
  CGCTCCCCGGACAAGCCGGGCAAGGGCTCCTACTGGACGCTGCACCCGGACTCCGGCAAC
  ATGTTCGAGAACGGCTGCTACTTGCGCCGCCAGAAGCGCTTCAAGTGCGAGAAGCAGCCG
  GGGGCCGGCGGCGGGGGCGGGAGCGGAAGCGGGGGCAGCGGCGCCAAGGGCGGCCCTGAG
  AGCCGCAAGGACCCCTCTGGCGCCTCTAACCCCAGCGCCGACTCGCCCCTCCATCGGGGT
  GTGCACGGGAAGACCGGCCAGCTAGAGGGCGCGCCGGCCCCCGGGCCCGCCGCCAGCCCC
  CAGACTCTGGACCACAGTGGGGCGACGGCGACAGGGGGCGCCTCGGAGTTGAAGACTCCA
  GCCTCCTCAACTGCGCCCCCCATAAGCTCCGGGCCCGGGGCGCTGGCCTCTGTGCCCGCC
  TCTCACCCGGCACACGGCTTGGCACCCCACGAGTCCCAGCTGCACCTGAAAGGGGACCCC
  CACTACTCCTTCAACCACCCGTTCTCCATCAACAACCTCATGTCCTCCTCGGAGCAGCAG
  CATAAGCTGGACTTCAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACG
  TTGCCCGCCAGCCTGCCTCTAGGCAGCGCCTCGGTGACCACCAGGAGCCCCATCGAGCCC
  TCAGCCCTGGAGCCGGCGTACTACCAAGGTGTGTATTCCAGACCCGTCCTAAACACTTCC
  TAGCTCCCGGGACTGGGGGGTTTGTCTGGCATAGCCATGCTGGTAGCAAGAGAGAAAAAA
  TCAACAGCAAACAAAACCACACAAACCAAACCGTCAACAGCATAATAAAATCCCAACAAC
  TATTTTTATTTCATTTTTCATGCACAACCTTTCCCCCAGTGCAAAAGACTGTTACTTTAT
  TATTGTATTCAAAATTCATTGTGTATATTACTACAAAGACAACCCCAAACCAATTTTTTT
  CCTGCGAAGTTTAATGATCCACAAGTGTATATATGAAATTCTCCTCCTTCCTTGCCCCCC
  TCTCTTTCTTCCCTCTTTCCCCTCCAGACATTCTAGTTTGTGGAGGGTTATTTAAAAAAA
  CAAAAAAGGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004496 unedited
  GTGCAGAATATTTGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGGTGGC
  GTTGGGCCCGCGCGGGCGCTCGGGTGACTGCAGCTGCTCAGCTCCCCTCCCCCGCCCCGC
  GCCGCGCGGCCGCCCGTCGCTTCGCACAGGGCTGGATGGTTGTATTGGGCAGGGTGGCTC
  CAGGATGTTAGGAACTGTGAAGATGGAAGGGCATGAAACCAGCGACTGGAACAGCTACTA
  CGCAGACACGCAGGAGGCCTACTCCTCCGTCCCGGTCAGCAACATGAACTCAGGCCTGGG
  CTCCATGAACTCCATGAACACCTACATGACCATGAACACCATGACTACGAGCGGCAACAT
  GACCCCGGCGTCCTTCAACATGTCCTATGCCAACCCGGGCCTAGGGGCCGGCCTGAGTCC
  CGGCGCAGTAACCGGCATGCCGGGGGGCTCGGCGGGCGCCATGAACAGCATGACTGCGGC
  CGGCGTGACGGCCATGGGTACGGCGCTGAGCCCGAGCGGCATGGGCGCCATGGGTGCGCA
  GCAGGCGGCCTCCATGAATGGCCTGGGCCCCTACGCGGCCGCCATGAACCCGTGCATGAG
  CCCCATGGCGTACGCGCCGTCCAAACCTGTGCCGCACCGCGCGGGCGGCGGCGGCGACGC
  CAAGACGTTCAAGCGCAGCTACCCGCACGCCAAGCCCGCCCTACTCGTACATCTTGCTCA
  TCACCATGGCCATCCAGCAGGCGCCCAGCAAGATGCTCACGCTGAGCCAGATCTTACAGT
  GGATCATGGACCTCTTTCCCTATTACCCGCCGAACCAGCATCGCTGGCAGAACTCCATCC
  CCACTCGCTGTCCTTTAATGACTGCGTCGTCCAGGTGGCACCCTCCCCCGACAAGCCGGG
  CCAAGGCTCCTACTGGACCCTTGACCCGGCTCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004496 unedited
  GGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTAACATTTGATTTATTTAAA
  GTCCTTAACTGCAAATGATCAAGAACATTTTCCACGGCTTAAAATCTGGTATTATTTTTG
  TTATTTAAATCAAAATATTTTTAATTACAATCACATTGATAAAAATTAACAATTTAATTT
  GTAATTTTAACCATCATTTAAAGCAATTTCAGCTGTAAAGAAAAAGAATAAACCATGCAA
  TAAATTAACATTGAGAAAGCAAAGAGAAATAATAATGAAACCCGTCTGGCTATACTAACA
  CCATGTCCAACTGTGGAAAGTGCATAACACTGGATGAAACAAGCACACGATGGCAATGAT
  TAAAATGGCCACTATCAATAGGATTTTTGAATCTTGGACCACGTTTTGAAATCCAGCTCC
  CTATAACTTATCTCTCCTCCAACATTGTAATATTCCCTTTTACTGAAAAAAAAATTCTTT
  AAATGGTATACACTCATAGAAACTAATTTTATGTGTTATGTGACCTCAGAATGACATGAC
  CATGGCACTCTGCAAAGCAAGAAGCAGAGTTCTTGAGGGCAATTCCTGAGGAATTGATTC
  CCACGGCCATCTGTGGGTAGAGAGGACAAAGGGGTTGGGGGCCTTGTAACCTTCGCCAAG
  CAACTCTTGAGAATGATCATGGATTCACCTATTTTTACCTCTAATGGTATGTAAAATACG
  GAGGATGGCTCCCATTTGACCATGTGCCTATTAAGCCCCTAACATATCCCTGTTTTCCAT
  TTAAGACCCGTGGTCTCCCCATGCCTCGAAAGGAAACTGGGAACAATGGCTCCCCAGTTC
  TAAAGGNGAAGGGCGGGCATATACACCACATGGGCTCACTACCTTCACAGCCCTAAGTGA
  ACACGCCCTCAGCCTCCAATCCCTTGGTAAACCCTGCGTCGCGCGTACCGCGGGGGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004496
• Insert Size: 3060 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004496.2, NP_004487.2
• RefSeq Size: 3124 bp
• RefSeq ORF: 1419 bp
• Locus ID: 3169
• Cytogenetics: 14q21.1
• Domains: FH
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]'