NIT2 (NM_020202) Human Untagged Clone

CAT#: SC108295

NIT2 (untagged)-Human nitrilase family, member 2 (NIT2)


  "NM_020202" in other vectors (6)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NIT2
Synonyms HEL-S-8a
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC108295 sequence for NM_020202 edited (data generated by NextGen Sequencing)
ATGACCTCTTTCCGCTTGGCCCTCATCCAGCTTCAGATTTCTTCCATCAAATCAGATAAC
GTCACTCGCGCTTGTAGCTTCATCCGGGAGGCAGCAACGCAAGGAGCCAAAATAGTTTCT
TTGCCGGAATGCTTTAATTCTCCATATGGAGCGAAATATTTTCCTGAATATGCAGAGAAA
ATTCCTGGTGAATCCACACAGAAGCTTTCTGAAGTAGCAAAGGAATGCAGCATATATCTC
ATTGGAGGCTCTATCCCTGAAGAGGATGCTGGGAAATTATATAACACCTGTGCTGTGTTT
GGGCCTGATGGAACTTTACTAGCAAAGTATAGAAAGATCCATCTGTTTGACATTGATGTT
CCTGGAAAAATTACATTTCAAGAATCTAAAACATTGAGTCCGGGTGATAGTTTCTCCACA
TTTGATACTCCTTACTGCAGAGTGGGTCTGGGCATCTGCTACGACATGCGGTTTGCAGAG
CTTGCACAAATCTACGCACAGAGAGGCTGCCAGCTGTTGGTATATCCAGGAGCTTTTAAT
CTGACCACTGGACCAGCCCATTGGGAGTTACTTCAGCGAAGCCGGGCTGTTGATAATCAG
GTGTATGTGGCCACAGCCTCTCCTGCCCGGGATGACAAAGCCTCCTATGTTGCCTGGGGA
CACAGCACCGTGGTGAACCCTTGGGGGGAGGTTCTAGCCAAAGCTGGCACAGAAGAAGCA
ATCGTGTATTCAGACATAGACCTGAAGAAGCTGGCTGAAATACGCCAGCAAATCCCCGTT
TTTAGACAGAAGCGATCAGACCTCTATGCTGTGGAGATGAAAAAGCCCTAA

Clone variation with respect to NM_020202.4
>OriGene 5' read for NM_020202 unedited
NGGTCGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATCGGCACGAGGCCGGATCTC
CAGCGCTCAGTCCGCGCCGCAGGTGGTGCTTGTCTGCAGAGTCATGACCTCTTTCCGCTT
GGCCCTCATCCAGCTTCAGATTTCTTCCATCAAATCAGATAACGTCACTCGCGCTTGTAG
CTTCATCCGGGAGGCAGCAACGCAAGGAGCCAAAATAGTTTCTTTGCCGGAATGCTTTAA
TTCTCCATATGGAGCGAAATATTTTCCTGAATATGCAGAGAAAATTCCTGGTGAATCCAC
ACAGAAGCTTTCTGAAGTAGCAAAGGAATGCAGCATATATCTCATTGGAGGCTCTATCCC
TGAAGAGGATGCTGGGAAATTATATAACACCTGTGCTGTGTTTGGGCCTGATGGAACTTT
ACTAGCAAAGTATAGAAAGATCCATCTGTTTGACATTGATGTTCCTGGAAAAATTACATT
TCAAGAATCTAAAACATTGAGTCCGGGTGATAGTTTCTCCACATTTGATACTCGTATGTA
CCAGATAAGTTTGCCTCTTTAGCAATCTCAGTAGAAGACAATCAGGTATTTATTTCTTTT
TTGTCTCTCTCCGATTTCTTCACATAACCTAACTGAAAGACCATAAGTGAGAAAGGCAGA
GAATCATCACAGATCTGGAAAGTTCGGGCTTATTTGAGAACTAANGATTTGACACGATTT
TGCCCTTTGATTTGATTGTAGCTTCCTGTTATGGCTTCCAGAGTATACCTATTAGGCTAC
CGTTGAGTACCTCCCATCTAGATCATAAGCATTCAATTAGAAATGAATTTCTCATCCTTA
GTCCGCTGAAGTAAATGCAGTCTTTATGAAATGAAGCCCAGTAAGAATGAGCTCGTAATT
A
>OriGene 3' read for NM_020202 unedited
CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCATTAAAGAATTTAATAGGGA
GTTGATTATGTTGTAGAATCATATCGTCCTATTCTGTGACACATTAAAAACATAAACTTT
AGGGCTTTTTCATCTCCACAGCATAGAGGTCTGATCGCTTCTGTCTAAAAACGGGGATTT
GCTGGCGTATTTCAGCCAGCTTCTTCAGGTCTATGTCTGAATACACGATTGCTTCTTCTG
TGCCAGCTTTGGCTAGAACCTCCCCCCAAGGGTTCACCACGGTGCTGTGTCCCCAGGCAA
CATAGGAGGCTTTGTCATCCCGGGCAGGAGAGGCTGTGGCCACATACACCTGATTATCAA
CAGCCCGGCTTCGCTGAAGTAACTCCCAATGGGCTGGTCCAGTGGTCAGATTAAAAGCTC
CTGGATATACCAACAGCTGGCAGCCTCTCTGTGCGTAGATTTGTGCAAGCTCTGCAAACC
GCATGTCGTAGCAGATGCCCAGACCCACTCTGCAGTAAGCTGTTTCCAAACAGAAAATGC
ACAAATGAAAAGTTAACTCCCTACAGCATGGTGTGGCAGGGGAAATCCTCCATGACATAA
TGATCACAAAACACTGACAGGGATAAATTAACACAGGACCTAAGGACAGAGATAATTTAC
AAGCTCATTCCTACTTGGACTTCATCTCATAAAGACATCATTTACATCAGCGGACTAAAG
ATGAGAAATTCATTCTAANTGAATGCTTATTATCTAGATGGGAGGTACTCAACTGTAGCC
TAATAGGTATACTCTGGAAGCCATACAGGGAGCTACAATCAAATCAAGGGCANAATCGTG
TCAAATCCTTAGTTCTCAAATAGCCCGAACTTCCAGACCTGGGATGATTCTCTGCCTTTC
TCACTTAGGGNCTTTCACTTAGGGTTATGGGAAGAAATCGGAGAGAGACAAAAAGAAAAA
AACCCCTGATGGCCTCTACTTGAAATGCCTAAGAAGA
Restriction Sites NotI-NotI     
ACCN NM_020202
ORF Size 831 bp
Insert Size 1500
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq NM_020202.2, NP_064587.1
RefSeq Size 1019
RefSeq ORF 831
Locus ID 56954
Domains CN_hydrolase
Gene Summary Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle. [UniProtKB/Swiss-Prot Function]

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