CKII alpha (CSNK2A1) (NM_177560) Human Untagged Clone

CAT#: SC108394

CSNK2A1 (untagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSNK2A1
• Synonyms: CK2A1; Cka1; Cka2; CKII; OCNDS
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_177560, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTATGAGATTCTGAAGGCCCTGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCC
  ATAATGTCATGATTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAGTTTTATCA
  TCCTGGCCAAGAATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCTGAGCTACTTGTAGACTAT
  CAGATGTACGATTATAGTTTGGATATGTGGAGTTTGGGTTGTATGCTGGCAAGTATGATCTTTCGGAAGG
  AGCCATTTTTCCATGGACATGACAATTATGATCAGTTGGTGAGGATAGCCAAGGTTCTGGGGACAGAAGA
  TTTATATGACTATATTGACAAATACAACATTGAATTAGATCCACGTTTCAATGATATCTTGGGCAGACAC
  TCTCGAAAGCGATGGGAACGCTTTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATT
  TCCTGGACAAACTGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAGCACCCCTA
  TTTCTACACTGTTGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCAGGGGGCAGTACGCCCGTC
  AGCAGCGCCAATATGATGTCAGGGATTTCTTCAGTGCCAACCCCTTCACCCCTTGGACCTCTGGCAGGCT
  CACCAGTGATTGCTGCTGCCAACCCCCTTGGGATGCCTGTTCCAGCTGCCGCTGGCGCTCAGCAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177560 unedited
  AACCGTAATTACCCCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCT
  ATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAG
  CGGCCGCGAATTCGGCACGAGGTGATATTCGATTTTACATGTATGAGATTCTGAAGGCCC
  TGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCCATAATGTCATGA
  TTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAGTTTTATCATC
  CTGGCCAAGAATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCTGAGCTACTTG
  TAGACTATCAGATGTACGATTATAGTTTGGATATGTGGAGTTTGGGTTGTATGCTGGCAA
  GTATGATCTTTCGGAAGGAGCCATTTTTCCATGGACATGACAATTATGATCAGTTGGTGA
  GGATAGCCAAGGTTCTGGGGACAGAAGATTTATATGACTATATTGACAAATACAACATTG
  AATTAGATCCACGTTTCAATGATATCTTGGGCAGACACTCTCGAAAGCGATGGGAACGCT
  TTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATTTCCTGGACAAAC
  TGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAGCACCCCTATT
  TCTACACTGTTGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCTAGGGGGCAGT
  ACGCCCGTCAGCAGCGCCAATATGATGTCAGGGATTTCTTCAGTGCCAACCCCTTCACCT
  TTGGACCTCTGGCAGCTCACCAGTGATTGCTGCTGCCAACCCCTTGNGATGCCTGATCCA
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177560
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177560.2, NP_808228.1
• RefSeq Size: 2522 bp
• RefSeq ORF: 768 bp
• Locus ID: 1457
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adherens junction, Tight junction, Wnt signaling pathway
• Gene Summary: 'Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Apr 2018]'
  Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR as well as the first coding exon compared to variant 1, resulting in an isoform (b) which is shorter at the N-terminus compared to isoform a.