ER81 (ETV1) (NM_004956) Human Untagged Clone

CAT#: SC108403

ETV1 (untagged)-Human ets variant 1 (ETV1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ETV1
• Synonyms: ER81
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004956 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCTTTCGCCTAGCGTGGCCTTC
  AGGTTGATAGAAGTCCAGATCCTGAGGAAATCTCCAGCTAAATGCTCAAAATATAAAAAC
  TGAGCTGAGATTTGCGAAGAGCAGCAGCATGGATGGATTTTATGACCAGCAAGTGCCTTA
  CATGGTCACCAATAGTCAGCGTGGGAGAAATTGTAACGAGAAACCAACAAATGTCAGGAA
  AAGAAAATTCATTAACAGAGATCTGGCTCATGATTCAGAAGAACTCTTTCAAGATCTAAG
  TCAATTACAGGAAACATGGCTTGCAGAAGCTCAGGTACCTGACAATGATGAGCAGTTTGT
  ACCAGACTATCAGGCTGAAAGTTTGGCTTTTCATGGCCTGCCACTGAAAATCAAGAAAGA
  ACCCCACAGTCCATGTTCAGAAATCAGCTCTGCCTGCAGTCAAGAACAGCCCTTTAAATT
  CAGCTATGGAGAAAAGTGCCTGTACAATGTCAGTGCCTATGATCAGAAGCCACAAGTGGG
  AATGAGGCCCTCCAACCCCCCCACACCATCCAGCACGCCAGTGTCCCCACTGCATCATGC
  ATCTCCAAACTCAACTCATACACCGAAACCTGACCGGGCCTTCCCAGCTCACCTCCCTCC
  ATCGCAGTCCATACCAGATAGCAGCTACCCCATGGACCACAGATTTCGCCGCCAGCTTTC
  TGAACCCTGTAACTCCTTTCCTCCTTTGCCGACGATGCCAAGGGAAGGACGTCCTATGTA
  CCAACGCCAGATGTCTGAGCCAAACATCCCCTTCCCACCACAAGGCTTTAAGCAGGAGTA
  CCACGACCCAGTGTATGAACACAACACCATGGTTGGCAGTGCGGCCAGCCAAAGCTTTCC
  CCCTCCTCTGATGATTAAACAGGAACCCAGAGATTTTGCATATGACTCAGAAGTGCCTAG
  CTGCCACTCCATTTATATGAGGCAAGAAGGCTTCCTGGCTCATCCCAGCAGAACAGAAGG
  CTGTATGTTTGAAAAGGGCCCCAGGCAGTTTTATGATGACACCTGTGTTGTCCCAGAAAA
  ATTCGATGGAGACATCAAACAAGAGCCAGGAATGTATCGGGAAGGACCCACATACCAACG
  GCGAGGATCACTTCAGCTCTGGCAGTTTTTGGTAGCTCTTCTGGATGACCCTTCAAATTC
  TCATTTTATTGCCTGGACTGGTCGAGGCATGGAATTTAAACTGATTGAGCCTGAAGAGGT
  GGCCCGACGTTGGGGCATTCAGAAAAACAGGCCAGCTATGAACTATGATAAACTTAGCCG
  TTCACTCCGCTATTACTATGAGAAAGGAATTATGCAAAAGGTGGCTGGAGAGAGATATGT
  CTACAAGTTTGTGTGTGATCCAGAAGCCCTTTTCTCCATGGCCTTTCCAGATAATCAGCG
  TCCACTGCTGAAGACAGACATGGAACGTCACATCAACGAGGAGGACACAGTGCCTCTTTC
  TCACTTTGATGAGAGCATGGCCTACATGCCGGAAGGGGGCTGCTGCAACCCCCACCCCTA
  CAACGAAGGCTACGTGTATTAACACAAGTGACAGTCAAGCAGGGCGTTTTTGCGCTTTTC
  CTTTTTTCTGCAAGATACAGAGAATTGCTGAATCTTTGTTTTATTTCTGTTGTTTGTATT
  TTATTTTTAAATAATAATACACAAAAAGGGGCTTTTCCTGTTGCATTATTCTATGGTCTG
  CCATGGACTGTGCACTTTATTTGAGGGTGGGTGGGAGTAATCTAAACATTTATTCTGTGT
  AACAGGAAGCTAATGGGTGAATGGGCAGAGGGATTTGGGGATTACTTTTTACTTAGGCTT
  GGGATGGGGTCCTACAAGTTTTGAGTATGATGAAACTATATCATGTCTGTTTGATTTCAT
  AACAACATAAGATAATGTTTATTTTATCGGGGTATCTATGGTACAGTTAATTTCACGTTG
  TGTAAATATCCACTTGGAGACTATTTGCCTTGGGCATTTTCCCTGTCATTTATGAGTCTC
  TGCAGGTGTACAAAAAAACCCCAATCXXXXXXXXXXXXXXXXXXXXXXXXXXTCATTTCT
  CTAAGTGGAGCAGTTATCAAGAAACCCAAACACCAAAATTGCTACTCTTCACATTTAATC
  CTACAAAAAGTACTCCAATTTCAAAATATGTATGTAACCTGCGATTTCAATGATTGTTGT
  TCATATACATCATGTATTATTTTGGCCCATTTTGGGCCTAAAAAAGAAAACTATGCCTTA
  AAAATCAGAACCTTTTCTCCCCACTATGCTTATGTGGCCATCTACAGCACTTAGAATAAA
  AACAGATGTTAAAATATTCAGTGAAAGTTTTATTGGAAAAAGGAATTGAGATATATAATT
  GAGATTTGGTGAAATTGAAGGAGAAAATTTAAGTGAGTCTTTAAAATATATTCTGAATGA
  AAACTGTATTGAGGATTCATTTTTGTTCCTTTTTTTTCTTTTTCTCTTTTCTCCTTTTTC
  TTCTTTTTAATAGTCTAGTTTTAGTCAGTCAGTGAGGAAGAATTGGGCCATGCTAACGTT
  ATCACAAGAGAACAATGGCAGAAATGGTATTAGTTATATAATATTTAAGGACAAACTATA
  TGTTTTGCTGTTTTAACGTAGTGACTCACTGAACTAAATACATAATTGACCAACATTAAG
  TGTATTTCCAATACAGAAGGGTTGAAAATATTACATTATAAACTCTTTTGAAAAATGTAT
  CTAAAATTTTTTAAGTTCTGTTTTGATTCCACTTTTTGGTTGAGTTTTTATGTTTTTGTT
  TTCAGGTAGATTAATAAATCTGGCAGCTGATTTCTGCAAAAAAAAAAAAAAAAAACTCGA
  C
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004956 unedited
  GGGGGCGCCGCNCNTTTCCTCTCCCNNNNGGGGTTCGAATTTGTNATACGACTTACTATA
  GGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCTTTCGCCTAGC
  GTGGCCTTCAGGTTGATAGAAGTCCAGATCCTGAGGAAATCTCCAGCTAAATGCTCAAAA
  TATAAAAACTGAGCTGAGATTTGCGAAGAGCAGCAGCATGGATGGATTTTATGACCAGCA
  AGTGCCTTACATGGTCACCAATAGTCAGCGTGGGAGAAATTGTAACGAGAAACCAACAAA
  TGTCAGGAAAAGAAAATTCATTAACAGAGATCTGGCTCATGATTCAGAAGAACTCTTTCA
  AGATCTAAGTCAATTACAGGAAACATGGCTTGCAGAAGCTCAGGTACCTGACAATGATGA
  GCAGTTTGTACCAGACTATCAGGCTGAAAGTTTGGCTTTTCATGGCCTGCCACTGAAAAT
  CAAGAAAGAACCCCACAGTCCATGTTCAGAAATCAGCTCTGCCTGCAGTCAAGAACAGCC
  CTTTAAATTCAGCTATGGAGAAAAGTGCCTGTACAATGTCAGTGCCTATGATCAGAAGCC
  ACAAGTGGGAATGAGGCCCTNCAACCNCNCCACACCATCCAGCACGCCAGTGTCCCCACT
  GCATCATGCATCTCCAAACTCAACTCATACACCGAAACCTGACCGGGCCTTNCCAGCTCA
  CCTNCCTTCATCGCAGTCCATACCAGATAGCAGCTACCCCATGGACCCACAGATTCGCCG
  CCAGCNTTCTGAACCCCTGTACTNCTTTCCTCCTTTGCCGACGATGCCANGGGAAGGACG
  TCCTATGTACCCACGCCAGATGTCTGAGCCAAACATCCCCTTCCACCACCAGGCCTTAGC
  AGGAGTCCCACGCCCCATGTATGACACCACACCATGGTTGCAGGGCGGCCGGCAAAGCTT
  TTCCCCTCCTGATGATTAACAGGAACCCNAGATTTGCATTGACTCAAAAGCCTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004956 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAGAAATAGCTGCCAGATTTATTAAT
  CTACCTGAAAACAAAAACATAAAAACTCAACCAAAAAGTGGAATCAAAACAGAACTTAAA
  AAATTTTAGATACATTTTTCAAAAGAGTTTATAATGTAATATTTTCAACCCTTCTGTATT
  GGAAATACACTTAATGTTGGTCAATTATGTATTTAGTTCAGTGAGTCACTACGTTAAAAC
  AGCAAAACATATAGTTTGTCCTTAAATATTATATAACTAATACCATTTCTGCCATTGTTC
  TCTTGTGATAACGTTAGCATGGCCCAATTCTTCCTCACTGACTGACTAAAACTAGACTAT
  TAAAAAGAAGAAAAAGGAGAAAAGAGAAAAAGAAAAAAAAGGAACAAAAATGAATCCTCA
  ATACAGTTTTCATTCAGAATATATTTTAAAGACTCACTTAAATTTTCTCCTTCAATTTCA
  CCAAATCTCAATTATATATCTCAATTCCTTTTTCCAATAAAACTTTCACTGAATATTTTA
  ACATCTGTTTTTATTCTAAGTGCTGTAGATGGCCACATAAGCATAGTGGGGAGAAAAGGT
  TCTGATTTTTAAGGCATAGTTTTCTTTTTTAGGCCCAAAATGGGCCAAAATAATACATGA
  TGTATATGAACAANCATCATTGAAATCGCAGGTTACATACATATTTTGAAATTGGAGTAC
  TTTTTGTAGGATTAAATGTGAAGAGTAGCAATTTTGGGTGTTTGGGTTTCNTTGATAACT
  GCTCACTTAAAGAATGAGCTGTGATCTGTGTANTTNTGAAAATGGGTANCTGNNGGGATC
  ATCTTTAANCATCTTTTCATGCTCANACGAAATGCTTTACAATAGGTTATTTTGACTTTG
  NGTTAGAATTTTTTTTTTTTGCCTTTGCATAAACCTTAAAAAAGTGTCTTCTGGATATAT
  GATGATGAACAAATCTGTCCTTGCTGATCTTACGACTTTCAAATCTTTCCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004956
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004956.3, NP_004947.2
• RefSeq Size: 6158 bp
• RefSeq ORF: 1434 bp
• Locus ID: 2115
• Cytogenetics: 7p21.2
• Domains: ETS, ETS_PEA3_N
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). This isoform contains the N-terminal TAD. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.