NDUFA10 (NM_004544) Human Untagged Clone

CAT#: SC108422

NDUFA10 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa (NDUFA10), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFA10
• Synonyms: CI-42k; CI-42KD; MC1DN22
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108422 sequence for NM_004544 edited (data generated by NextGen Sequencing)
  ATGGCCTTGCGGCTCCTGAAGCTGGCAGCGACGTCCGCGTCCGCCCGGGTCGTGGCGGCG
  GGCGCCCAGCGCGTGAGAGGAATTCATAGCAGTGTGCAGTGCAAGCTGCGCTATGGAATG
  TGGCATTTCCTACTTGGGGATAAAGCAAGCAAAAGACTGACAGAACGCAGCAGAGTGATA
  ACTGTAGATGGCAATATATGTACTGGAAAAGGCAAACTTGCAAAAGAAATAGCAGAGAAA
  CTAGGCTTCAAGCACTTTCCTGAAGCGGGGATTCATTATCCAGACAGTACCACAGGAGAT
  GGGAAGCCCCTCGCCACCGACTATAATGGCAACTGTAGTTTGGAGAAATTTTACGATGAT
  CCGAGAAGCAATGATGGCAACAGTTACCGCCTGCAGTCCTGGTTGTACAGCAGTCGCCTG
  CTGCAGTACTCAGATGCCTTGGAGCACTTGCTGACCACAGGACAAGGTGTTGTGTTGGAG
  CGCTCCATCTTCAGTGACTTTGTGTTCCTGGAGGCGATGTACAACCAGGGATTCATCCGA
  AAGCAGTGTGTGGACCACTACAACGAGGTGAAGAGCGTCACCATCTGCGATTACCTGCCC
  CCCCACCTGGTGATTTACATCGATGTGCCCGTTCCAGAGGTCCAGAGGCGGATTCAGAAG
  AAAGGAGATCCACATGAAATGAAGATCACCTCTGCCTATCTACAGGACATTGAGAATGCC
  TATAAGAAAACCTTTCTCCCTGAGATGAGTGAAAAATGTGAGGTTTTACAATATTCTGCA
  AGGGAAGCTCAAGATTCAAAAAAGGTGGTAGAGGACATTGAATACCTGAAGTTCGATAAA
  GGGCCGTGGCTCAAGCAGGACAATCGCACTTTATACCACCTGCGATTACTGGTTCAGGAT
  AAGTTTGAGGTGCTGAATTACACAAGCATTCCTATCTTTCTCCCGGAAGTCACCATTGGA
  GCTCATCAGACTGACCGTGTCTTACATCAGTTCAGAGAGCTGCCGGGCCGCAAGTACAGC
  CCTGGGTACAACACCGAGGTGGGAGACAAGTGGATCTGGCTGAAGTGA
  
  Clone variation with respect to NM_004544.3
  105 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004544 unedited
  TCAGTATATTTGTAATACGCACTCACTATAGGGCGGCCGCGCAATTCGCACGAGGGTAGC
  CATGGCCTTGCGGCTCCTGAAGCTGGCAGCGACGTCCGCGTCCGCCCGGGTCGTGGCGGC
  GGGCGCCCAGCGCGTGAGAGGAATTCATAGCAGTGTGCAGTGCAAGCTGCGCTATGGAAT
  GTGGCATTTCCTACTTGGGGATAAAGCAAGCAAAAGACTGACAGAACGCAGCAGAGTGAT
  AACTGTAGATGGCAATATATGTACTGGAAAAGGCAAACTTGCAAAAGAAATAGCAGAGAA
  ACTAGGCTTCAAGCACTTTCCTGAAGCGGGGATTCATTATCCAGACAGTACCACAGGAGA
  TGGGAAGCCCCTCGCCACCGACTATAATGGCAACTGTAGTTTGGAGAAATTTTACGATGA
  TCCGAGAAGCAATGATGGCAACAGTTACCGCCTGCAGTCCTGGTTGTACAGCAGTCGCCT
  GCTGCAGTACTCAGATGCCTTGGAGCACTTGCTGACCACAGGACAAGGTGTTGTGTTGGA
  GCGCTCCATCTTCAGTGACTTTGTGTTCCTGGAGGCGATGTACAACCAGGGATTCATCCG
  AAAGCAGTGTGTGGACCACTACAACGAGGTGAAGAGCGTCACCATCTGCGATTACCTGCC
  CCCNCACCTGGTGATTTACATCGATGTGCCCGTTCCAGAGGTCCAGAGGCGGATTCAGAA
  GAAGGAGATCACATGAAATGAAAATCACCTCTGCCTATCTACAGGACATTGAGAATGCCT
  ATAAGAAAACCTTTCTNCCTGAGATGAGGTGAAAATGTGAGGTTTTACAATATTCTGCAG
  GGGAAGCTTCAGATTCAAAAAGGTGGTAGAAGACAATGAATACCTGAAGTNCGATAAAGG
  GCGTGGCTCAAGCACGACAATGCCCTTTATACACTGCGATACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004544 unedited
  ATGGACCGCGGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCCCATGGAAACA
  CTTTTATTTCTGGAAGTCAGAAGAAAAACAATGTGCACAACCTGAATGACACAGAGCGGC
  AGCGCTGAAACCACAGGGGCTGCCGAGAGCTGGCCTTTCACAGCAGACCACTGTTTTCCA
  GTGAGAATGGTGGGCCATTCCAAAACAAAGCTAAAGGGTTCCAAACATCCAGAATGGAAG
  CTGCTTCCCCCAACTCCATTACCTATACTACAGGATGGATTGCTTTTTGTGAGACCCCTT
  CTTCCACTGTGCAATTTTTGCATTATTTACCCTCCCCCGATCTTAAAGCTATATGGCGTC
  CAGGAGAGTGCGGCTGATGCAGCTTGGCCATCACTGTGATGCAGCTGGAGCAGAAGGCGG
  CCCGTTCACTTCAGCCAGATCCACTTGTCTCCCACCTCGGTGTTGTACCCAGGGCTGTAC
  TTGCGGCCCGGCAGCTCTCTGAACTGATGTAAGACACGGTCAGTCTGATGAGCTCCAATG
  GTGACTTCCGGGAGAAAGATAGGAATGCTTGTGTAATTCAGCACCTCAAACTTATCCTGA
  ACCAGTAATCGCAGGTGGTATAAAGTGCGATTGTCCTGCTTGAGCCACGCCCCTTTATCG
  AACTCCAGGTATTCAATGTCCTCTACCACCTTTTTTGAATCTTGAGCTTCCCTTGCAGAA
  TATTGTAAAACCTCACATTTTTCACTCATCTCAGGGAAAAAGGTTTCTTATAGGCATTCT
  CAATGTCCTGTANATAGCAGAGGTGATCTTCATTCCTGGGGATCTCTTTCTTCTGAACCG
  NCTCTGCACTNTTGGACGGCCCATCGATGTAATCCCAGGTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004544
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004544.2, NP_004535.1
• RefSeq Size: 1557 bp
• RefSeq ORF: 1068 bp
• Locus ID: 4705
• Cytogenetics: 2q37.3
• Domains: dNK
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The protein encoded by this gene is a component of 42 kDa complex I, the first enzyme complex in the electron transport chain of mitochondria. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. A mutation in this gene was found in an individual with Leigh syndrome. [provided by RefSeq, Apr 2016]'