MRPS25 (NM_022497) Human Untagged Clone

CAT#: SC108433

MRPS25 (untagged)-Human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPS25
• Synonyms: MRP-S25; RPMS25
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108433 sequence for NM_022497 edited (data generated by NextGen Sequencing)
  ATGCCCATGAAGGGCCGCTTCCCCATCCGCCGCACCCTGCAATATCTGAGCCAGGGGAAC
  GTGGTGTTCAAGGACTCCGTGAAGGTCATGACAGTGAATTACAACACGCATGGGGAGCTG
  GGCGAGGGCGCCAGGAAGTTTGTGTTTTTCAACATACCTCAGATTCAATACAAAAACCCT
  TGGGTGCAGATCATGATGTTTAAGAACATGACGCCGTCACCCTTCCTGCGATTCTACTTA
  GATTCTGGGGAGCAGGTCCTGGTGGATGTGGAGACCAAGAGCAATAAGGAGATCATGGAG
  CACATCAGAAAAATCTTGGGGAAGAATGAGGAAACCCTCAGGGAAGAGGAGGAGGAGAAA
  AAGCAGCTTTCTCACCCAGCCAACTTCGGCCCTCGAAAGTACTGCCTGCGGGAGTGCATC
  TGTGAAGTGGAAGGGCAGGTGCCCTGCCCCAGCCTGGTGCCATTACCCAAGGAGATGAGG
  GGGAAGTACAAAGCCGCTCTGAAAGCCGATGCCCAGGACTAA
  
  Clone variation with respect to NM_022497.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022497 unedited
  TATAGGCGGCCCGCGAATTCGCACGAGGCTGGCTCTGGGGAGAAGCCGCGTGAGATCCGC
  GCGGGTGCTAGCTAGTCCTTTCTCGTCGCTGCTCGGCTCGCGGCCCGTGGGGTCGGCCCC
  GCCACCGTTGCCGCCATGCCCATGAAGGGCCGCTTCCCCATCCGCCGCACCCTGCAATAT
  CTGAGCCAGGGGAACGTGGTGTTCAAGGACTCCGTGAAGGTCATGACAGTGAATTACAAC
  ACGCATGGGGAGCTGGGCGAGGGCGCCAGGAAGTTTGTGTTTTTCAACATACCTCAGATT
  CAATACAAAAACCCTTGGGTGCAGATCATGATGTTTAAGAACATGACGCCGTCACCCTTC
  CTGCGATTCTACTTAGATTCTGGGGAGCAGGTCCTGGTGGATGTGGAGACCAAGAGNCAT
  AAGGAGATCATGGAGCACATCAGAAAAATCTTGGGGAAGAATGAGGAAACCCTCAGGNAA
  GAGGANGAGGAGAANAAGCAGCTTTCTCACCCAGCCAACTTCGCCCTCGAAAGTACTGNC
  TGCGGNAGTGCATCTGTGAGNNGGAAGGGGGCAGTGCCCTGCCCAGCCTGGTGCCCTTAC
  CCAGGAAATGAGGGGGGAATACAAAACCCGTCTGAAAGCCGATGCCCAGACTAAGCCCAN
  GTACTGNGGCTGGGGGAAAATGGTTCCTACCTGGGGAAATGGAAAGGGGATTCCTTGGCC
  CGGAACCCCCCCGGTTTTTGCCTGGAAACTGGGAAAACTTTTGTTTAAAGGCTTTCAACC
  TGGTAAAGGGTGGGGAAAGAGGGGGGGNAAAACCAACCCCAAAAAAAAACCCCGGCTGGG
  GAAAAAAACCTTCTATAACCTTTACGGTTTGGGTTTAAAAAAAAGGGGCCTGAAAAAAGA
  GTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022497 unedited
  NTTTCTCTGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTGAAAAACTGTTAAAATTTAAGGTTGGNGAATAACATTTGGGAAGTAGTAGAACT
  TTTGCATATAAAAAATGGCTTTTTCTTTAGGAAAAGTATAATCCAATTTATTTTAATGCA
  CCAATAGCATTCATGGGACTCAAATGCTGGCTGCCACACACAAACTCAAGGGCTTGGAAT
  ATTTTNTCTACAGTTCTCTCTGTTCTACGGATTATCTTGTAAACCAGGGTTCAACTAGTT
  TTATAACTGAAAAGCTGCACATTTTTCACAGTACAAGCCGAAGTTTTTACCTGTAGTTTG
  GACTTCTCTGTTAAAATGTAACTGCATTACCAGCCCTTTTAAAGGCATCTATCTATCAAA
  GGAAAATTTGGGGGTTAAATTTTCTTGGGACCGTTTCTGTAACCTTTGCCCTTCACAATA
  TAAAAAATATTGGTTTTGCCATTACATTTTAATGCCAGGTTTAAAACCTGTTGAAAGCTG
  CAGCTTTATACAGCTTTCTTCTCCCTGTGAAATCACTTTGTTTATGTGATGGCATTTAAA
  AAATAGCATGTTCTTTTTAAACTGAATTTTATATCTAATCAATGTCTTCAGTCTTGAAGA
  AGAATTTGCATTGTTGCGTTTGTATATAGAGTATTGCAGCGCATGAATTACCTTTATGCA
  TTTTATTAAATGCTGTTTCAATGTGCCATTATTGTTGGGGCCTAATCCACCACCCTAATG
  AGGTTTATCCTATTAAAGGGGAATTAAAGACTAACATGCCTCCCTGCCCTTAAATTTTTC
  CACATGAAAAGAAGAAAGGCCCTGGCCCCCAAACCAATGCACGGCTTTTTTTGGGTTATG
  GAACAAGAAGCATCCTGGGGGGCCCGGCCTTTAACAGTCTTGGCGCCCCACCTTAGCCTT
  AGCGGCCAGAGGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022497
• ORF Size: 522 bp
• Insert Size: 5270
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_022497.3, NP_071942.1
• RefSeq Size: 4574
• RefSeq ORF: 522
• Locus ID: 64432
• Domains: L51_S25_CI-B8
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the supported protein.