NNT (NM_012343) Human Untagged Clone

CAT#: SC108450

NNT (untagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NNT
• Synonyms: GCCD4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108450 sequence for NM_012343 edited (data generated by NextGen Sequencing)
  ATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCTACTTAGCAATTTG
  GGGTCCTGTAAGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATTTTATACTCACCAA
  GAACTGTGGTGTAAAGCGCCTGTAAAACCAGGAATTCCATATAAGCAACTGACTGTTGGA
  GTCCCCAAAGAGATATTCCAAAATGAGAAGCGAGTGGCATTGTCTCCTGCTGGTGTTCAG
  AACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATCGGGTGCGGGCGAAGCTTCCAAG
  TTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGGGCAAAGGAAGTGCTG
  GCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAACATTAGGTGTTCAT
  GAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTTACCCAGCCCAAAAT
  CCAGAGTTGCTAAATAAACTTTCCCAAAGAAAAACTACAGTTCTGGCAATGGACCAGGTT
  CCAAGAGTCACAATTGCTCAGGGATATGATGCGCTAAGCTCCATGGCCAACATTGCGGGT
  TATAAGGCTGTTGTCCTAGCAGCAAATCATTTTGGACGTTTTTTTACTGGTCAGATCACA
  GCTGCTGGAAAAGTTCCTCCAGCTAAGATTCTGATAGTTGGTGGTGGTGTTGCTGGGCTT
  GCTTCTGCAGGCGCAGCAAAGTCGATGGGTGCAATTGTTCGAGGATTTGACACAAGAGCT
  GCAGCTTTGGAACAGTTCAAGTCTCTTGGTGCTGAGCCCTTGGAGGTGGACTTGAAGGAA
  TCTGGTGAGGGACAAGGAGGATATGCAAAAGAGATGTCCAAAGAGTTCATTGAAGCTGAA
  ATGAAACTCTTTGCTCAACAATGCAAGGAGGTAGACATCCTTATCAGCACAGCACTTATT
  CCAGGTAAAAAAGCTCCAGTTTTATTTAATAAAGAAATGATTGAGTCAATGAAGGAAGGT
  TCAGTTGTTGTGGATTTAGCTGCTGAGGCTGGTGGAAACTTTGAAACCACTAAGCCAGGA
  GAACTCTACATTCATAAGGGAATTACTCACATAGGCTACACAGACCTGCCCAGCCGAATG
  GCCACTCAGGCCAGCACCCTATATTCCAACAACATCACCAAACTCCTGAAGGCCATCAGC
  CCGGACAAAGATAATTTTTATTTTGATGTGAAAGATGACTTTGACTTTGGTACGATGGGT
  CATGTCATTAGAGGAACTGTAGTGATGAAAGATGGTAAAGTGAWTTTCCCAGCTCCCACA
  CCGAAAAATATTCCTCAAGGTGCCCCAGTAAAACAGAAGACAGTGGCTGAGCTGGAAGCT
  GAAAAAGCAGCTACCATTACACCCTTCAGGAAGACAATGTCAACGGCTTCTGCATATACA
  GCAGGTCTCACAGGGATACTGGGTTTGGGCATTGCGGCTCCCAATCTAGCCTTTTCTCAG
  ATGGTGACCACTTTTGGCTTGGCTGGCATTGTGGGGTATCATACCGTCTGGGGAGTGACC
  CCTGCTCTCCACTCACCACTGATGTCTGTGACAAATGCAATCTCAGGGCTGACTGCAGTT
  GGTGGGTTGGCACTGATGGGAGGACATTTGTATCCTTCCACAACTTCTCAGGGCCTTGCT
  GCTCTTGCTGCATTCATATCCTCTGTCAACATTGCAGGTGGCTTTCTGGTGACTCAGAGA
  ATGCTGGACATGTTCAAGCGTCCCACTGACCCCCCAGAATACAACTACCTGTACCTGCTC
  CCTGCCGGCACCTTTGTTGGTGGATATTTAGCTGCCCTCTACAGTGGTTATAACATTGAA
  CAGATCATGTACCTAGGCTCGGGTTTGTGCTGTGTCGGTGCCTTGGCTGGCCTCTCCACC
  CAGGGAACAGCACGTCTTGGCAATGCACTGGGCATGATTGGGGTTGCTGGAGGACTGGCA
  GCCACCCTCGGAGTCCTAAAACCGGGCCCAGAATTACTAGCTCAGATGTCTGGAGCGATG
  GCTTTGGGTGGTACCATTGGATTGACAATTGCCAAACGCATCCAGATTTCTGATTTACCT
  CAATTAGTTGCTGCTTTTCACAGTTTAGTGGGTTTGGCAGCTGTACTTACTTGCATAGCT
  GAGTACATTATAGAATATCCACATTTTGCTACGGATGCAGCAGCAAATCTCACCAAGATT
  GTGGCCTACCTCGGCACTTACATTGGTGGCGTCACCTTTAGTGGGTCTCTCATTGCCTAT
  GGAAAATTGCAGGGTCTCCTGAAATCTGCCCCTCTCCTACTGCCTGGAAGGCACTTACTC
  AATGCAGGCTTACTGGCTGCTAGTGTGGGCGGGATAATCCCATTCATGGTGGACCCAAGC
  TTTACTACTGGCATCACCTGTCTGGGTTCAGTGTCTGCTCTCTCTGCTGTCATGGGTGTG
  ACTTTGACAGCTGCTATTGGGGGTGCTGACATGCCCGTCGTTATCACTGTGCTGAACAGC
  TACTCAGGCTGGGCCCTGTGTGCAGAGGGCTTCCTGCTCAACAACAATCTGCTGACCATC
  GTGGGTGCACTCATAGGCTCGTCTGGTGCTATCCTGTCATACATCATGTGTGTGGCAATG
  AATCGCTCCCTGGCTAATGTGATTCTTGGAGGCTATGGCACCACTTCAACAGCTGGTGGA
  AAACCCATGGAAATTTCTGGCACACATACGGAAATCAACCTTGACAATGCAATTGACATG
  ATTCGAGAAGCTAATAGCATTATTATTACACCAGGCTATGGTCTCTGTGCAGCCAAAGCT
  CAATACCCCATTGCTGATTTGGTAAAGATGCTCACTGAGCAAGGCAAAAAAGTCAGGTTT
  GGAATTCACCCAGTTGCAGGCCGAATGCCTGGTCAGCTTAATGTGCTGCTGGCTGAGGCT
  GGTGTGCCATATGACATTGTGTTGGAAATGGATGAGATCAACCATGATTTTCCAGATACT
  GATTTGGTCCTTGTAATTGGAGCTAATGACACTGTTAATTCAGCAGCTCAAGAAGATCCC
  AACTCTATTATTGCAGGCATGCCAGTCCTTGAGGTCTGGAAATCAAAGCAGGTGATTGTT
  ATGAAGAGGTCTTTGGGTGTTGGCTATGCTGCAGTGGACAATCCAATCTTCTACAAACCT
  AACACGGCCATGCTTCTAGGTGATGCCAAGAAAACATGTGACGCGCTCCAGGCGAAAGTT
  AGAGAATCCTATCAGAAGTAA
  
  Clone variation with respect to NM_012343.3
  1304 t=>w
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012343 unedited
  GGTTTTAGGATTTGTNATCCGACTTCATATAGGGCGGCCGCGCAATTCGCACGAGGGGCA
  CAGCCCAGGCTGTCAGCCTCCCGGCCCAGTGATTTGCCTTCAAGGAAACTGGGGAGTCAG
  AAAATTGGGAACTCATATCAACATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTC
  GTGTCCTCTACTTAGCAATTTGGGGTCCTGTAAGGGTCTACGTGTGAAGAAGGATTTTTT
  ACGAACATTTTATACTCACCAAGAACTGTGGTGTAAAGCGCCTGTAAAACCAGGAATTCC
  ATATAAGCAACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAGCGAGTGGC
  ATTGTCTCCTGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATC
  GGGTGCGGGCGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAAT
  CCAAGGGGCAAAGGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGT
  TAATCCAACATTAGGTGTTCATGAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAG
  TTTTATTTACCCAGCCCAAAATCCAGAGTTGCTAAATAAACTTTCCCAAAGAAAAACTAC
  AGTTCTGGCAATGGACCAGGTTCCAAGAGTCACAATTGCTCAGGGATATGATGCGCTAAG
  CTCCATGGCCAACATTGCGGGTTATAAAGCTGTTGTCCTAGCAGCANATCATTTTGGACG
  TTTTTTTACTGGTCAGATCACAGCTGCTGGGAAAGTTCCTCCAGCTAAGATCTGAAGTTG
  GTGGTGGGTGTTGCTGGGCTTGCTTCTGCAGCGCAGCANATCGAGGGTGCAATTGTCGAG
  GATTGACACAGACTGCAGCTTGGAACAGTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012343 unedited
  CCTTCGTTCTATTTCCCCACCTTCCACACCAGGNTTTGTGTGNANGAAAACCGCAATCCG
  AGGCCNGGTTTTTTTCCTTTTTTTTTTTTTGCCAACTGTTTTACTCTTTTATAGCCAAAG
  TTGAATTCCACTCAAGTGCACTTAATATAGTACTACACATTATAAGTTGATCGAAATTAA
  AGAGATCTCCAATTAGAAATGGGATCTTCAAAAATACTATTACTAATTTTCAAGCTGGAT
  GTTTCATTTTTTTTCTAATGAAACATATAAAGCAAATGATTTGTTCCCAGCCTCTCTGAA
  AGATTATTTAGCCCTTTAATGTATTAAAATAGTAGTGAGAAAATGTGCCCTTGAAATATA
  GACTTTGAAGTACAATTTAAAAAATTAAAACTGACTTGATATGATTAATTCAAACTGCGG
  TTGAATTGCAAGGGGACAGCTCATAAGCTCATAAAAGGTTCAAATTAGCCACATTTGTTG
  AAAAAAAAAACATAAAATGCAGGTGCCTGCAATGGTTACATTGTGTCAGATATTTCAGAA
  GCATGTATAACGCTTGCCTTGAAATCCTAAACACTTACCTTTTTTAAACATTTTTTGGCT
  ACTGAGGAGGTATCAAAAATTCAACAGGACTACCTCTAACATGACGGCTGTTCTTTCTCC
  CTTTATTCAACACCTTTTGACTTCCTACACAAAAAAAATATGGCCTAGAAAGACATTTAG
  AATCTTTCCATTCCTTTTTAAAAATTGAGGGATTACCGTATTTTGAAAATTTTTTTCTAT
  ACCGCTCTTTGCCTTTGCTTTTTTCCACCCTTTATTTCCTTCCACAAGTTCTCTTTCCCA
  ATGGTCATTCCCCTTGTTTACGGGACCTCTCCTCGCGTTGTTCCCAAACATTGCAAGGTG
  GGTCTCTTAACTTAACGCTGGGTCCTCTATAATAAATCCCGGGAGGAATTTGTTAATCCC
  CGCCCCGAAAGGGTAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012343
• ORF Size: 3261 bp
• Insert Size: 4620
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_012343.2, NP_036475.2
• RefSeq Size: 4600
• RefSeq ORF: 3261
• Locus ID: 23530
• Domains: PNTB, AlaDh_PNT_C, AlaDh_PNT_N
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism
• Gene Summary: This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform.