Paxillin (PXN) (NM_002859) Human Untagged Clone

CAT#: SC108478

PXN (untagged)-Human paxillin (PXN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PXN
• Synonyms: FLJ16691
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108478 sequence for NM_002859 edited (data generated by NextGen Sequencing)
  ATGGACGACCTCGACGCCCTGCTGGCGGACTTGGAGTCTACCACCTCCCACATCTCCAAA
  CGGCCTGTGTTCTTGTCGGAGGAGACCCCCTACTCATACCCAACTGGAAACCACACATAC
  CAGGAGATTGCCGTGCCACCCCCCGTCCCCCCACCCCCGTCCAGCGAGGCCCTCAATGGC
  ACAATCCTTGACCCCTTAGACCAGTGGCAGCCCAGCGGCTCCCGATTCATCCACCAGCAG
  CCTCAGTCCTCATCACCTGTGTACGGCTCCAGTGCCAAAACTTCCAGTGTCTCCAACCCT
  CAGGACAGTGTTGGCTCTCCGTGCTCCCGAGTGGGTGAGGAGGAGCACGTCTACAGCTTC
  CCCAACAAGCAGAAATCAGCTGAGCCTTCACCCACCGTAATGAGCACGTCCCTGGGCAGC
  AACCTTTCTGAACTCGACCGCCTGCTGCTGGAACTGAACGCTGTACAGCATAACCCGCCA
  GGCTTCCCTGCAGATGAGGCCAACTCAAGCCCCCCGCTTCCTGGGGCCCTGAGCCCCCTC
  TATGGTGTCCCAGAGACTAACAGCCCCTTGGGAGGCAAAGCTGGGCCCCTGACGAAAGAG
  AAGCCTAAGCGGAATGGGGGCCGGGGCCTGGAGGACGTGCGGCCCAGTGTGGAGAGTCTC
  TTGGATGAACTGGAGAGCTCCGTGCCCAGCCCCGTCCCTGCCATCACTGTGAACCAGGGC
  GAGATGAGCAGCCCGCAGCGCGTCACCTCCACCCAACAGCAGACACGCATCTCGGCCTCC
  TCTGCCACCAGGGAGCTGGACGAGCTGATGGCTTCGCTGTCGGATTTCAAGTTCATGGCC
  CAGGGGAAGACAGGGAGCAGCTCACCCCCTGGGGGGCCCCCGAAGCCCGGGAGCCAGCTG
  GACAGCATGCTGGGGAGCCTGCAGTCTGACCTGAACAAGCTGGGGGTCGCCACAGTCGCC
  AAAGGAGTCTGCGGGGCCTGCAAGAAGCCCATCGCCGGGCAGGTTGTGACCGCCATGGGG
  AAGACGTGGCACCCCGAGCACTTCGTCTGCACCCACTGCCAGGAGGAGATCGGATCCCGG
  AACTTCTTCGAGCGGGATGGACAGCCCTACTGTGAAAAGGACTACCACAACCTCTTCTCC
  CCGCGCTGCTACTACTGCAACGGCCCCATCCTGGATAAAGTGGTGACAGCCCTTGACCGG
  ACGTGGCACCCTGAACACTTCTTCTGTGCACAGTGTGGAGCCTTCTTTGGTCCCGAAGGG
  TTCCACGAGAAGGACGGCAAGGCCTACTGTCGCAAGGACTACTTCGACATGTTCGCACCC
  AAGTGTGGCGGCTGCGCCCGGGCCATCCTGGAGAACTATATCTCAGCCCTCAACACGCTG
  TGGCATCCTGAGTGCTTTGTGTGCCGGGAATGCTTCACGCCATTCGTGAACGGCAGCTTC
  TTCGAGCACGACGGGCAGCCCTACTGTGAGGTGCACTACCACGAGCGGCGCGGCTCGCTG
  TGTTCTGGCTGCCAGAAGCCCATCACCGGCCGCTGCATCACCGCCATGGCCAAGAAGTTC
  CACCCCGAGCACTTCGTCTGTGCCTTCTGCCTCAAGCAGCTCAACAAGGGCACCTTCAAG
  GAGCAGAACGACAAGCCTTACTGTCAGAACTGCTTCCTCAAGCTCTTCTGCTAG
  
  Clone variation with respect to NM_002859.3
  217 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002859 unedited
  NAAAAAAAGAGAAAAAAACTTNNNNTNNNNNNNNNGTTTTTACCCCGCCCCTTGCCCCAA
  AGGGCGGTAGGCGTGTACGGTGGGAAGTCTATATAAGCAGAGCTCATTTAGGTGACACTA
  TAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCTCGCGGC
  GGAAAAGTTGCGGGGCATAGACGAGCGGCCCCGGGACGGGCAGCTAGCGCGACCCTGAGC
  CGGCGCCCGTGGTCCGGCCATGGACGACCTCGACGCCCTGCTGGCGGACTTGGAGTCTAC
  CACCTCCCACATCTCCAAACGGCCTGTGTTCTTGTCGGAGGAGACCCCCTACTCATACCC
  AACTGGAAACCACACATACCAGGAGATTGCCGTGCCACCCCCCGTCCCCCCACCCCCGTC
  CAGCGAGGCCCTCAATGGCACAATCCTTGACCCCTTAGACCAGTGGCAGCCCAGCGGCTC
  CCGATTCATCCACCAGCAGCCTCAGTCCTCATCACCTGTGTACGGCTCCAGTGCCAAAAC
  TTCCAGTGTCTCCAACCCTCAGGACAGTGTTGGCTCTCCGTGCTCCCGAGTGGGTGAGGA
  GGAGCACGTCTACAGCTTCCCCAACAAGCAGAAATCAGCTGAGCCTTCACCCACCGTAAT
  GAGCACGTCCCTGNGCAGCAACCTTTCTGAACTCGACCGCCTGCTGCTGGAACTGAACGC
  TGTACAGCATAACCCGCCAGGCTTCCCTGCAGATGAGGCCAACTCAAGCCCCCCGCTTTC
  TGGGGCCCCTGAGCCCCCTCTATGGTGTCCCAGAGACCTACAGCCCCTTGGGNAGCAAAG
  CTGGCCCCCTGACGAAAGAGAAGCCTAAGCGGAATGGGGGCCGGGGCCTNGAAGNACGTG
  CCGCCCCATGTGGNAGAGTCTCTTGGATGAACTGAAGAGCTCGTGCCCCACCCCGTTCCT
  GCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002859 unedited
  GGGGAATCAGCGTGNACAGGCCCGTTAGCGANGATCGAAAACCTTTTTTTTTTTTTGAAA
  ACATGGGCAAACTTTATTGGGGCATAAATCACAGGGAATTTGAAATGGGGAAAAGCCAGC
  TTGGAAGTTTACAGGAGAAAAAATAAAATCAAAATCAAATTTTCAAATAACCACTGACTC
  AGAGAATGGACCCCAGAAACCCCAGTCCCTCTTGGGGTAATATTTAGGGTGGGTCACTAT
  AAACAGAGCGGTAAGGCCTGCGGGTGAAAGTGGAGACATCAGACCTTTGGAGGGGCTGGG
  CTGACCTTCCCCCAAAAAGGAGGGACCCTCTTCGGAGACCCCAGCACACTCGGCTTCTGC
  CAGGAGGCCAGTGGAGTGGTTTGGACTGTCACCATTAGACCCCGGTGGGGCCCCTTTCTC
  CTCATCAGGAGCCCAAGGCCTGGCTCCTCTTTCATCCTTGGTAAAGAGCTCTGGGGGGTT
  TCCCCAGAGGAGCCTGTCCCTCTGCCCCAAATCCTATCATGCTCCCTTATGACCTTCTTT
  CCAGACCCCCTGAGTGCCGATCCCACCTGTGAAAGGGGAGGAGCAGATCTGGGGAAGGGA
  TATGCCCAGAGGAAATCAACAAGACCAAAGAGACGTCAGAGTCCTGATGGCCAAAATCAG
  ACACCAGCTTTCCTGAGAAGGCAGAAGAGAGAGCCCCATAAAAGCAGTGGGGGCTCAGAA
  GGTTCAGTGGGTTCACAGGGGAGCAAGGCTCCTGAGGGCTACTCAGACAGAAGCACCCCC
  CAAAAATACTTCCCCTTTTCCTCCCCGGAAACAGACAGATAAGGTTCAAAATTTAGGCCC
  CAAAGGGGGCACACCCTTCAACCAATTCTATGAAAAACTTGTTGGCCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002859
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002859.1, NP_002850.1
• RefSeq Size: 3595 bp
• RefSeq ORF: 1674 bp
• Locus ID: 5829
• Cytogenetics: 12q24.23
• Domains: LIM, Paxillin
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway
• Gene Summary: 'This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1 (also known as isoform alpha).