PPP1R7 (NM_002712) Human Untagged Clone

CAT#: SC108479

PPP1R7 (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 7 (PPP1R7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1R7
• Synonyms: SDS22
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108479 sequence for NM_002712 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGAACGCGGCGCGGGGCAGCAACAGTCGCAGGAGATGATGGAGGTTGACAGG
  CGGGTCGAGTCTGAAGAATCCGGCGATGAAGAAGGGAAGAAACACAGCAGTGGCATCGTG
  GCCGACCTCAGTGAACAGAGCCTGAAGGATGGGGAGGAGCGGGGGGAGGAGGACCCAGAA
  GAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGGAT
  GTTGATTTGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAGTG
  AAGACTCTCTGCCTCCGCCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTACAG
  AGTCTTCGAGAGCTGGATCTTTACGACAACCAGATCAAGAAGATTGAGAATCTGGAGGCG
  CTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATCTGCTGAGAAACATCGAAGGGGTT
  GACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAATCAGTAAAATTGAG
  AACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGGGCA
  ATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAATT
  ACTAAACTTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCAAC
  CGGCTGACCAAGATCGAGGGTCTGCAGAACCTGGTGAACCTGCGGGAGCTGTACCTTAGC
  CACAATGGCATCGAGGTCATCGAGGGCCTGGAGAACAATAACAAACTCACGATGTTGGAC
  ATTGCATCAAATAGAATCAAAAAGATTGAAAATATCAGCCATCTAACAGAGCTGCAAGAG
  TTCTGGATGAACGACAATCTCCTTGAGAGCTGGAGCGACCTCGACGAGCTGAAGGGAGCC
  AGGAGCCTGGAGACAGTGTACCTGGAGCGGAACCCCTTGCAGAAGGACCCCCAGTACCGG
  CGGAAGGTCATGCTCGCCCTCCCCTCCGTGCGGCAGATCGATGCCACGTTCGTCAGGTTC
  TGA
  
  Clone variation with respect to NM_002712.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002712 unedited
  TGTCACAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGCAGCCA
  ACATGGCGGCGGAACGCGGCGCGGGGCAGCAACAGTCGCAGGAGATGATGGAGGTTGACA
  GGCGGGTCGAGTCTGAAGAATCCGGCGATGAAGAAGGGAAGAAACACAGCAGTGGCATCG
  TGGCCGACCTCAGTGAACAGAGCCTGAAGGATGGGGAGGAGCGGGGGGAGGAGGACCCAG
  AAGAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGG
  ATGTTGATTTGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAG
  TGAAGACTCTCTGCCTCCGCCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTAC
  AGAGTCTTCGAGAGCTGGATCTTTACGACAACCAGATCAAGAAGATTGAGAATCTGGAGG
  CGCTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATCTGCTGAGAAACATCGAAGGGG
  TTGACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAATCAGTAAAATTG
  AGAACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGGG
  CAATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAA
  TTACTAAACTTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCA
  ACCGGCTGACCAAGATCCGAGGTCTGCAGAACCTGGTGAACCTGCCGGAGCTGTACCTTA
  GCCACAATGGCATCCGAGTCATCGAGGGCCTGNAGAACATTACAAACTCACGATGTGGNA
  CATTGCATCAAAAGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002712 unedited
  ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGACAGTGTGGCAAGATT
  TAATTGCAACGGCATCTGAAAATGGTGTGTGGCGTCGCGCGCGCCAGCTATCGTCAGTGC
  CTTTATTGCCATTGGGTTTGTGACTGTTGATATAGTGACGACCTCAGGAGCAACAGGTGG
  GTTAAAAACCCGTGGCTGGGCAGTTCTTCCGAGGAGAGGGACCACATGAGGAGCCAAGAA
  GGACTCAGAACCTGACGAACGTGGCATCGATCTGCCGCACGGAGGGGAGGGCGAGCATGA
  CCTTCCGCCGGTACTGGGGGTCCTTCTGCAAGGGGTTCCGCTCCAGGTACACTGTCTCCA
  GGCTCCTGGCTCCCTTCAGCTCGTCGAGGTCGCTCCAGCTCTCAAGGAGATTGTCGTTCA
  TCCAGAACTCTTGCAGCTCTGTTAGATGGCTGATATTTTCAATCTTTTTGATTCTATTTG
  ATGCAATGTCCAACATCGTGAGTTTGTTATTGTTCTCCAGGCCCTCGATGACCTCGATGC
  CATTGTGGCTAAAGTACAGCTCCCGCAGGTTCACCAGGTTCTGCAGACCCTCGATCTTGG
  TCAGCCGGTTGCTCTGCATACTGAGGACTGTCACGTTGGTGACCCCATCCAGGTTCTGAA
  GTTTAGTAACTTTGTTTCTCCCAAAACAAACTCTCCAGGTTGGGAAAGGTGCGAATTTTT
  TCATTGCCCCGGAGCTCGTAAACTCCACCCTCACACTCGTAACGACGTGAGTTGTTTCAT
  TCTCAATTCTCCTTGCTCTATGTGCACCATAACAATTCTTTCATTCTACATACTTTTCAC
  CCTTCTAATGTTTTTCTCCTATTAAAGACTATCTCAAACTCCCACTTTGTTTTCTTCCAT
  TTTCACCTCTCCCCCGTGTGCCATAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002712
• Insert Size: 1360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002712.1, NP_002703.1
• RefSeq Size: 1299 bp
• RefSeq ORF: 1083 bp
• Locus ID: 5510
• Cytogenetics: 2q37.3
• Domains: LRR, LRR_TYP, LRRcap, LRR_SD22
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'This gene encodes a protein subunit that regulates the activity of the serine/threonine phosphatase, protein phosphatase-1. The encoded protein is required for completion of the mitotic cycle and for targeting protein phosphatase-1 to mitotic kinetochores. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as sds22alpha1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.