GATA3 (NM_002051) Human Untagged Clone

CAT#: SC108486

GATA3 (untagged)-Human GATA binding protein 3 (GATA3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GATA3
• Synonyms: HDR; HDRS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002051 edited
  GGCGCCGTCTTGATACTTTCAGAAAGAATGCATTCCCTGTAAAAAAAAAAAAAAAAAATA
  CTGAGAGAGGGAGAGAGAGAGAGAAGAAGAGAGAGAGACGGAGGGAGAGCGAGACAGAGC
  GAGCAACGCAATCTGACCGAGCAGGTCGTACGCCGCCGCCTCCTCCTCCTCTCTGCTCTT
  CGCTACCCAGGTGACCCGAGGAGGGACTCCGCCTCCGAGCGGCTGAGGACCCCGGTGCAG
  AGGAGCCTGGCTCGCAGAATTGCAGAGTCGTCGCCCCTTTTTACAACCTGGTCCCGTTTT
  ATTCTGCCATACCCAGTTTTTGGATTTTTGTCTTCCCCTTCTTCTCTTTGCTAAACGACC
  CCTCCAAGATAATTTTTAAAAAACCTTCTCCTTTGCTCACCTTTGCTTCCCAGCCTTCCC
  ATCCCCCCACCGAAAGCAAATCATTCAACGACCCCCGACCCTCCGACGGCAGGAGCCCCC
  CGACCTCCCAGGCGGACCGCCCTCCCTCCCCGCGCGCGGGTTCCGGGCCCGGCGAGAGGG
  CGCGAGCACAGCCGAGGCCATGGAGGTGACGGCGGACCAGCCGCGCTGGGTGAGCCACCA
  CCACCCCGCCGTGCTCAACGGGCAGCACCCGGACACGCACCACCCGGGCCTCAGCCACTC
  CTACATGGACGCGGCGCAGTACCCGCTGCCGGAGGAGGTGGATGTGCTTTTTAACATCGA
  CGGTCAAGGCAACCACGTCCCGCCCTACTACGGAAACTCGGTCAGGGCCACGGTGCAGAG
  GTACCCTCCGACCCACCACGGGAGCCAGGTGTGCCGCCCGCCTCTGCTTCATGGATCCCT
  ACCCTGGCTGGACGGCGGCAAAGCCCTGGGCAGCCACCACACCGCCTCCCCCTGGAATCT
  CAGCCCCTTCTCCAAGACGTCCATCCACCACGGCTCCCCGGGGCCCCTCTCCGTCTACCC
  CCCGGCCTCGTCCTCCTCCTTGTCGGGGGGCCACGCCAGCCCGCACCTCTTCACCTTCCC
  GCCCACCCCGCCGAAGGACGTCTCCCCGGACCCATCGCTGTCCACCCCAGGCTCGGCCGG
  CTCGGCCCGGCAGGACGAGAAAGAGTGCCTCAAGTACCAGGTGCCCCTGCCCGACAGCAT
  GAAGCTGGAGTCGTCCCACTCCCGTGGCAGCATGACCGCCCTGGGTGGAGCCTCCTCGTC
  GACCCACCACCCCATCACCACCTACCCGCCCTACGTGCCCGAGTACAGCTCCGGACTCTT
  CCCCCCCAGCAGCCTGCTGGGCGGCTCCCCCACCGGCTTCGGATGCAAGTCCAGGCCCAA
  GGCCCGGTCCAGCACAGGCAGGGAGTGTGTGAACTGTGGGGCAACCTCGACCCCACTGTG
  GCGGCGAGATGGCACGGGACACTACCTGTGCAACGCCTGCGGGCTCTATCACAAAATGAA
  CGGACAGAACCGGCCCCTCATTAAGCCCAAGCGAAGGCTGTCTGCAGCCAGGAGAGCAGG
  GACGTCCTGTGCGAACTGTCAGACCACCACAACCACACTCTGGAGGAGGAATGCCAATGG
  GGACCCTGTCTGCAATGCCTGTGGGCTCTACTACAAGCTTCACAATATTAACAGACCCCT
  GACTATGAAGAAGGAAGGCATCCAGACCAGAAACCGAAAAATGTCTAGCAAATCCAAAAA
  GTGCAAAAAAGTGCATGACTCACTGGAGGACTTCCCCAAGAACAGCTCGTTTAACCCGGC
  CGCCCTCTCCAGACACATGTCCTCCCTGAGCCACATCTCGCCCTTCAGCCACTCCAGCCA
  CATGCTGACCACGCCCACGCCGATGCACCCGCCATCCAGCCTGTCCTTTGGACCACACCA
  CCCCTCCAGCATGGTCACCGCCATGGGTTAGAGCCCTGCTCGATGCTCACAGGGCCCCCA
  GCGAGAGTCCCTGCAGTCCCTTTCGACTTGCATTTTTGCAGGAGCAGTATCATGAAGCCT
  AAACGCGATGGATATATGTTTTTGAAGGCAGAAAGCAAAATTATGTTTGCCACTTTGCAA
  AGGAGCTCACTGTGGTGTCTGTGTTCCAACCACTGAATCTGGACCCCATCTGTGAATAAG
  CCATTCTGACTCATATCCCCTATTTAACAGGGTCTCTAGTGCTGTGAAAAAAAAAATGCT
  GAACATTGCATATAACTTATATTGTAAGAAATACTGTACAATGACTTTATTGCATCTGGG
  TAGCTGTAAGGCATGAAGGATGCCAAGAAGTTTAAGGAATATGGGAGAAATAGTGTGGAA
  ATTAAGAAGAAACTAGGTCTGATATTCAAATGGACAAACTGCCAGTTTTGTTTCCTTTCA
  CTGGCCACAGTTGTTTGATGCATTAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002051 unedited
  CAGTATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGTGGCGCCG
  TCTTGATACTTTCAGAAAGAATGCATTCCCTGTAAAAAAAAAAAAAAAAAATACTGCAGA
  GAGGGAGNCAGAGAGAGAGAAGAAGAGAGAGAGACGGAGGGAGAGCGAGACAGAGCGAGC
  AACGCAATCTGACCGAGCAGGTCGTACGCCGCCGCCTCCTCCTCCTCTCTGCTCTTCGCT
  ACCCAGGTGACCCGAGGAGGGACTCCGCCTCCGAGCGGCTGAGGACCCCGGTGCAGAGGA
  GCCTGGCTCGCAGAATTGCAGAGTCGTCGCCCCTTTTTACAACCTGGTCCCGTTTTATTC
  TGCCATACCCAGTTTTTGGATTTTTGTCTTCCCCTTCTTCTCTTTGCTAAACGACCCCTC
  CAAGATAATTTTTAAAAAACCTTCTCCTTTGCTCACCTTTGCTTCCCAGCCTTCCCATCC
  CCCCACCGAAAGCAAATCATTCAACGACCCCCGACCCTTCGACGGCAGGAGCCCCCCGAC
  CTCCCAGGCGGACCGCCCTCCCTCCCCGCGCGCGGGTTCCGGGCCCGGCGAGAGGGCGCG
  AGCCCAGCCGACGCCATGGAGGTGACGGTGGACCACCCGGGCTGGGTGAGCCACCACTAC
  CCTGCCGTGTTACGGGGCATCACCCGGACACGCACCACCCGGGCCTCAAGCCCTTCTACC
  TGGGCGCGGGGCAATACCCGGTGCCGGAGGAAGGGGATGATCCTTTTTACCTCGAAGGGT
  AAGGGAACCACGTCCGGCCTTACTACGAAACTCGGTCAGGGCCCCGGGGCTAAGGTACCT
  TTCGACCCACCCCGGGAGACAGGTGGCGCGGCCCCCTCTTGCTTTTGGATCCCCTCCCTT
  GGTGGGACGGGGGATAACCTTGGGAGTCCACACCTTGGCTCCCCGGGAAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002051 unedited
  NGGCCTTTTTTCAGCTCACCACTGTGGCCGTGAAAGAACAAACTGGCAGTTTGTCCATTT
  GAATATCAGACCTAGTTTCTTCTTATTTCCACACTATTTCTCCCATATTCCTTAAACTTC
  TTGGCATCCTTCATGCCTTACAGCTACCCAGATGCAATAAAGTCATTGTACAGTATTTCT
  TACAATATAAGTTATATGCAATGTTCAGCATTTTTTTTTTTCACAGCACTAGAGACCCTG
  TTAAATAGGGGATATGAGTCAGAATGGCTTATTCACAGATGGGGTCCAGATTCAGTGGTT
  GGAACACAGACACCACAGTGAGCTCCTTTGCAAAGTGGCAAACATAATTTTGCTTTCTGC
  CTTCAAAAACATATATCCATCGCGTTTAGGCTTCATGATACTGCTCCTGCAAAAATGCAA
  GTCGAAAGGGACTGCAGGGACTCTCGCTGGGGGCCCTGTGAGCATCGAGCAGGGCTCTAA
  CCCATGGCGGTGACCATGCTGGAGGGGTGGTGTGGTCCAAAGGACAGGCTGGATGGCGGG
  TGCATCGGCGTGGGCGTGGTCAGCATGTGGCTGGAGTGGCTGAAGGGCGAGATGTGGCTC
  ANGGAGGACATGTGTCTGGAGAGGGCGGCCGGGTTAAACGAGCTGTTCTTGGGGAAGTCC
  TCCAGTGAGTCATGCACTTTTTTGCACTTTTTGGATTTGCTAGACATTNTTCGGTTNCTG
  GTCTGGATGCCTTCCTTCTTATAGTCAGGGGTCTGTTAATATTGTGAAGCTTGTAGTAGA
  GCCCACAGGCATTGCAGACAGGGTCCCCATTGGCATTCCTCCTTCANAGTGTGTTGTGGT
  GGTCTGACAGTTTCCACAGGACGTNCCTGCTCTNCTGGCTGCAGACAGCCTTCGCTTGGG
  CTTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002051
• Insert Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002051.2, NP_002042.1
• RefSeq Size: 3067 bp
• RefSeq ORF: 1332 bp
• Locus ID: 2625
• Cytogenetics: 10p14
• Domains: GATA
• Protein Families: Adult stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Gene Summary: 'This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the mid-coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1.