EIF2B1 (NM_001414) Human Untagged Clone

CAT#: SC108577

EIF2B1 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa (EIF2B1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF2B1
• Synonyms: EIF2B; EIF2BA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108577 sequence for NM_001414 edited (data generated by NextGen Sequencing)
  ATGGACGACAAGGAGTTAATTGAATACTTTAAGTCTCAGATGAAAGAAGATCCTGACATG
  GCCTCAGCAGTGGCTGCCATCCGGACGTTGCTGGAGTTCTTGAAGAGAGATAAAGGGGAG
  ACAATCCAGGGTCTGAGGGCGAATCTCACCAGTGCCATAGAAACCCTGTGTGGTGTGGAC
  TCCTCTGTGGCAGTGTCCTCTGGCGGGGAGCTCTTCCTCCGCTTCATCAGTCTTGCCTCC
  CTGGAATACTCCGATTACTCCAAATGTAAAAAGATCATGATTGAGCGGGGAGAACTTTTT
  CTCAGGAGAATATCACTGTCAAGAAACAAAATTGCAGATCTGTGCCATACTTTCATCAAA
  GATGGAGCGACAATATTGACTCACGCCTACTCCAGAGTGGTCCTGAGAGTCCTGGAAGCA
  GCCGTGGCGGCCAAGAAGCGATTTAGTGTATACGTCACAGAGTCACAGCCTGATTTGTCA
  GGTAAGAAAATGGCCAAAGCCCTCTGCCACCTCAACGTCTCTGTCACTGTGGTGCTAGAT
  GCTGCTGTCGGCTACATCATGGAGAAAGCAGATCTTGTCATAGTTGGTGTTGAAGGAGTT
  GTTGAAAACGGAGGAATTATTAACAAGATTGGAACCAACCAGATGGCTGTGTGTGCCAAA
  GCACAGAACAAACCTTTCTATGTGGTTGCAGAAAGTTTCAAGTTTGTCCGGCTCTTTCCA
  CTAAACCAGCAAGACGTCCCAGATAAGTTTAAGTATAAGGCAGACACTCTCAAGGTCGCG
  CAGACTGGACAAGACCTCAAAGAGGAGCATCCGTGGGTCGACTACACTGCCCCTTCCTTA
  ATCACTCTGCTGTTTACAGACCTGGGCGTGCTGACACCCTCAGCAGTCAGCGATGAGCTC
  ATCAAGCTCTATCTGTAA
  
  Clone variation with respect to NM_001414.3
  520 c=>t;590 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001414 unedited
  TAGAGTCGAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACNGGGGGGTGC
  GGAGGTGTGCAGCGCGCTGTCAGACTGGCTCGCAGGCGGCGCGGCCGGCGGACCCGTTCG
  AGACAGCGCGGGCGGCTCGGGTCCCCTGGGGCTCCGCAGCAGGAGGACGCCATGGACGAC
  AAGGAGTTAATTGAATACTTTAAGTCTCAGATGAAAGAAGATCCTGACATGGCCTCAGCA
  GTGGCTGCCATCCGGACGTTGCTGGAGTTCTTGAAGAGAGATAAAGGGGAGACAATCCAG
  GGTCTGAGGGCGAATCTCACCAGTGCCATAGAAACCCTGTGTGGTGTGGACTCCTCTGTG
  GCAGTGTCCTCTGGCGGGGAGCTCTTCCTCCGCTTCATCAGTCTTGCCTCCCTGGAATAC
  TCCGATTACTCCAAATGTAAAAAGATCATGATTGAGCGGGGAGAACTTTTTCTCAGGAGA
  ATATCACTGTCAAGAAACAAAATTGCAGATCTGTGCCATACTTTCATCAAAGATGGAGCG
  ACAATATTGACTCACGCCTACTCCAGAGTGGTCCTGAGAGTCCTGGGAGCAGCCGTGGCG
  GCCAAGAAGCGATTTAGTGTATACGTCACAGAGTCACAGCCTGATTTGTCAGGTAAGAAA
  AATGCCAAAGCCCTCTGCCACCTCAACGTCTCTGTCACTGTGGTGCTAGATGCTGCTGTC
  GGCTACATCATGGAGAAAGCAGATCTTGTCATAGTTGGTGTTGAAGGAGTTGTTGAAAAA
  CGAGGAATTATTAANCAGATTGGACCCCACAGATGGCTGTGTGTGCCAAAGCACAGAACA
  AACCTTTCTATGTGGTTGCAGAAAGGTTTAAAGTTTGTCCGGTCTTTTCACTAAACCAGC
  AGGACGTCCCAGATAAAATTAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001414 unedited
  AACTTCCAGGGCAGGNAAGGCACTGGGGAGGGGTCACAGGATGCCACCCGGGATCTGTTC
  AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTC
  TTTCATAGAGTTTATTTGAAAAGATGCTGAATTTATTCCCAAGTATAATTTTAAAAAGCT
  GTTTAGGACCCAAACATATTTAAACATCTCTTACACATACAGAATTTCAGTTTACAAATA
  TTCCAGAAGGCATTTTCTTAAGCAGTGAACCATGCAGAATACTGTTGCTAAACATCGTTT
  CTGCCTTCCTCGTGTTCTCTTGGTAGTTTTTACTGTTTGGCTTGATCCTGCTGAGGAATG
  TGCTTACCACTGGAAGCCAGACGCAGATTCGGCAAGTGTTTCTTGCCTCTTACAAGGCAC
  CGCGTGTAACGTCCTGACCAGCTGTTGGTCGTTTGTGGCAGAGGGGTATGGCTGCTTTTC
  GCCTGCATCTTGCGTGCTTTAGGCAGATCAGTCTGGAGTGCAGGGGAGGATGAAGACAGG
  TGGACTTGGGCTCATCTTTCATCAGTTAAGTAGCCAATTATCTAACTTGTATTTCTGGAA
  ACTGAAAAGAAAGTTTCTAGAAACCGTAAGAAAATTTAAGTTGGGATTTAGAATAGCTGA
  CACATTTTTAGATGGGACTGAAATGAGTAAAAAAGGTTCTCCAGATGTATGATTNTAAGT
  CCTTACTCCATAAATCTTCATTAAACACATCTCAGTTTTGGCCTGACTACTGGGGGTGTC
  AAGCAGCTACTCACCCTGCCTNCACTACGTAAGCTGCACCTGGCAGGAAAGGCTTACAGN
  TAAGATAGAGCTTGATGACTATCCTGATGCTTGAGGTGTANACGCCCAGTCTGTACAGCA
  AGTGATTAGAAAGGGGCCAGGTATCACCACGAGCTCTTTTTTGGGGTTGTCACTGCGACT
  TTGAAAGGGTGCTATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001414
• Insert Size: 1650 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001414.1, NP_001405.1
• RefSeq Size: 1658 bp
• RefSeq ORF: 918 bp
• Locus ID: 1967
• Cytogenetics: 12q24.31
• Domains: IF-2B
• Gene Summary: 'This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009]'