IL10RA (NM_001558) Human Untagged Clone

CAT#: SC108765

IL10RA (untagged)-Human interleukin 10 receptor, alpha (IL10RA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL10RA
• Synonyms: CD210; CD210a; CDW210A; HIL-10R; IL-10R1; IL10R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108765 sequence for NM_001558 edited (data generated by NextGen Sequencing)
  ATGCTGCCGTGCCTCGTAGTGCTGCTGGCGGCGCTCCTCAGCCTCCGTCTTGGCTCAGAC
  GCTCATGGGACAGAGCTGCCCAGCCCTCCGTCTGTGTGGTTTGAAGCAGAATTTTTCCAC
  CACATCCTCCACTGGACACCCATCCCAAATCAGTCTGAAAGTACCTGCTATGAAGTGGCG
  CTCCTGAGGTATGGAATAGAGTCCTGGAACTCCATCTCCAACTGTAGCCAGACCCTGTCC
  TATGACCTTACCGCAGTGACCTTGGACCTGTACCACAGCAATGGCTACCGGGCCAGAGTG
  CGGGCTGTGGACGGCAGCCGGCACTCCAACTGGACCGTCACCAACACCCGCTTCTCTGTG
  GATGAAGTGACTCTGACAGTTGGCAGTGTGAACCTAGAGATCCACAATGGCTTCATCCTC
  GGGAAGATTCAGCTACCCAGGCCCAAGATGGCCCCCGCGAATGACACATATGAAAGCATC
  TTCAGTCACTTCCGAGAGTATGAGATTGCCATTCGCAAGGTGCCGGGAAACTTCACGTTC
  ACACACAAGAAAGTAAAACATGAAAACTTCAGCCTCCTAACCTCTGGAGAAGTGGGAGAG
  TTCTGTGTCCAGGTGAAACCATCTGTCGCTTCCCGAAGTAACAAGGGGATGTGGTCTAAA
  GAGGAGTGCATCTCCCTCACCAGGCAGTATTTCACCGTGACCAACGTCATCATCTTCTTT
  GCCTTTGTCCTGCTGCTCTCCGGAGCCCTCGCCTACTGCCTGGCCCTCCAGCTGTATGTG
  CGGCGCCGAAAGAAGCTACCCAGTGTCCTGCTCTTCAAGAAGCCCAGCCCCTTCATCTTC
  ATCAGCCAGCGTCCCTCCCCAGAGACCCAAGACACCATCCACCCGCTTGATGAGGAGGCC
  TTTTTGAAGGTGTCCCCAGAGCTGAAGAACTTGGACCTGCACGGCAGCACAGACAGTGGC
  TTTGGCAGCACCAAGCCATCCCTGCAGACTGAAGAGCCCCAGTTCCTCCTCCCTGACCCT
  CACCCCCAGGCTGACAGAACGCTGGGAAACGGGGAGCCCCCTGTGCTGGGGGACAGCTGC
  AGTAGTGGCAGCAGCAATAGCACAGACAGCGGGATCTGCCTGCAGGAGCCCAGCCTGAGC
  CCCAGCACAGGGCCCACCTGGGAGCAACAGGTGGGGAGCAACAGCAGGGGCCAGGATGAC
  AGTGGCATTGACTTAGTTCAAAACTCTGAGGGCCGGGCTGGGGACACACAGGGTGGCTCG
  GCCTTGGGCCACCACAGTCCCCCGGAGCCTGAGGTGCCTGGGGAAGAAGACCCAGCTGCT
  GTGGCATTCCAGGGTTACCTGAGGCAGACCAGATGTGCTGAAGAGAAGGCAACCAAGACA
  GGCTGCCTGGAGGAAGAATCGCCCTTGACAGATGGCCTTGGCCCCAAATTCGGGAGATGC
  CTGGTTGATGAGGCAGGCTTGCATCCACCAGCCCTGGCCAAGGGCTATTTGAAACAGGAT
  CCTCTAGAAATGACTCTGGCTTCCTCAGGGGCCCCAACGGGACAGTGGAACCAGCCCACT
  GAGGAATGGTCACTCCTGGCCTTGAGCAGCTGCAGTGACCTGGGAATATCTGACTGGAGC
  TTTGCCCATGACCTTGCCCCTCTAGGCTGTGTGGCAGCCCCAGGTGGTCTCCTGGGCAGC
  TTTAACTCAGACCTGGTCACCCTGCCCCTCATCTCTAGCCTGCAGTCAAGTGAGTGA
  
  Clone variation with respect to NM_001558.3
  459 a=>g;1051 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001558 unedited
  NNCCTTCGGATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCGCGCA
  GGCCGGCTCCGCTCCGGCCCCGGACGATGCGGCGCGCCCAGGATGCTGCCGTGCCTCGTA
  GTGCTGCTGGCGGCGCTCCTCAGCCTCCGTCTTGGCTCAGACGCTCATGGGACAGAGCTG
  CCCAGCCCTCCGTCTGTGTGGTTTGAAGCAGAATTTTTCCACCACATCCTCCACTGGACA
  CCCATCCCAAATCAGTCTGAAAGTACCTGCTATGAAGTGGCGCTCCTGAGGTATGGAATA
  GAGTCCTGGAACTCCATCTCCAACTGTAGCCAGACCCTGTCCTATGACCTTACCGCAGTG
  ACCTTGGACCTGTACCACAGCAATGGCTACCGGGCCAGAGTGCGGGCTGTGGACGGCAGC
  CGGCACTCCAACTGGACCGTCACCAACACCCGCTTCTCTGTGGATGAAGTGACTCTGACA
  GTTGGCAGTGTGAACCTAGAGATCCACAATGGCTTCATCCTCGGGAAGATTCAGCTACCC
  AGGCCCAAGATGGCCCCCGCGAATGACACATATGAAAGCATCTTCAGTCACTTCCGAGAG
  TATGAGATTGCCATTCGCAAGGTGCCGGGAAACTTCACGTTCACACACAAGANAGTAAAA
  CATGAAAACTTCAGCCTCCTAACCTCTGGAGAAAGTGGGAGAAGTTCTGTGTCCAGGTGA
  AACCATCTGTCGCTTCCCGAAGTACAGGNNGATGTGGTCTAAGAGGAGTGCATCTCCCTC
  ACCAGCAGTATTTCACCGTGACCAACGTCATCATCTTCTTTCCCTTGTCCTGCTGCTCTC
  CGGAGCCCTCGCTACTGCCTGGCCTNCAGCTGTATGTGCCGCGCCGAAGAGCTACCCAGT
  GTCTGCTCTTCAGAAGCCAGCCCTTCTCTTCATAACCACGTCCTTCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001558 unedited
  GGGAAATAGCTTGNCCGCGGCCGCTATCTANGATCGAGTTTTTTTTTTTTTTTTTTGTGG
  TCTTGACAATTTATTGGAATAAAAAACATGTATCACTTATTGACAGAGCTTTGTGTTCAA
  GGCACATTCATACCCATTTCCTCAGCAGAACCTCACATCCCTATGAATTAGACAAGTCAG
  TCATTATTCTGCAGATGAGGAAACTGAGGCTCCAAGAGGATAAGTGACTTCTCCAAGGTC
  ATACCACTGGAAACAGCAAAGTCAGAGCTAGAATTTCGGGGCTCCTGAGATATCCAGAAT
  TCTTTCACTGTGCAATGCTGCCTCTCCAATAAATAAATGAACAAAATAAATAAATAAAGC
  TTTCAAGGGAACCCTGAGGAATCCTCCCTCAAGCCTGCCTCCAGAGGGAGTTCAGTTACC
  AGACTGGCACCTAAGAGTACTTTGGCAGAGGGGTGGGGGTCATGGCTGGATTCCCTGGGC
  CAGAATATCTGTTTTCAGAATGCTCCAGAGAAAGGCAGGGCTGAGATGACTCTGGATGGC
  ACGCACAGGCCTGTCCCCTCACAGCTTCCATGCCTGTGGACTGAACTGCCTTTGTTTGAA
  GGCAGAGTCCAAATGGCCTATGGGGAGGCAGGGCCCATAATGCCCCCTTCTGGGGCTGTG
  TCTGATACTGTCTTGGCATGACACCATGGACTCCCATTGGATCTGTTAGCCTCTGGCATA
  AGAAACAAGCCAGCTTGGTAGTTCACGGACAGTATCTGCATGCTCCCAGGACAGCTTGGC
  TAATACCCAGCAGACATGGAGGGTCCCCTAAAGTGGAGGTCCTTATTTCACAGATGGGAG
  ATTGAGGCCCTCTGACAGTGGGTAGTCCAAATCACCCAGCTGACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001558
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001558.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001558.2, NP_001549.1
• RefSeq Size: 3649 bp
• RefSeq ORF: 1737 bp
• Locus ID: 3587
• Cytogenetics: 11q23.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Gene Summary: 'The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]'
  Transcript Variant: This variant (1) is the protein-coding transcript.