ENPP1 (NM_006208) Human Untagged Clone

CAT#: SC108777

ENPP1 (untagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENPP1
• Synonyms: ARHR2; COLED; M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006208, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCGCGACGGCTGCGCGGGGGGCGGGAGCCGCGGCGGCGAGGGCGGGCGCGCTCCCCGGGAGGGCC
  CGGCGGGGAACGGCCGCGATCGGGGCCGCAGCCACGCTGCCGAGGCGCCCGGGGACCCGCAGGCGGCCGC
  GTCCTTGCTGGCCCCTATGGACGTGGGGGAGGAGCCGCTGGAGAAGGCGGCGCGCGCCCGCACTGCCAAG
  GACCCCAACACCTATAAAGTACTCTCGCTGGTATTGTCAGTATGTGTGTTAACAACAATACTTGGTTGTA
  TATTTGGGTTGAAACCAAGCTGTGCCAAAGAAGTTAAAAGTTGCAAAGGTCGCTGTTTCGAGAGAACATT
  TGGGAACTGTCGCTGTGATGCTGCCTGTGTTGAGCTTGGAAACTGCTGTTTAGATTACCAGGAGACGTGC
  ATAGAACCAGAACATATATGGACTTGCAACAAATTCAGGTGTGGTGAGAAAAGGTTGACCAGAAGCCTCT
  GTGCCTGTTCAGATGACTGCAAGGACAAGGGCGACTGCTGCATCAACTACAGTTCTGTGTGTCAAGGTGA
  GAAAAGTTGGGTAGAAGAACCATGTGAGAGCATTAATGAGCCACAGTGCCCAGCAGGGTTTGAAACGCCT
  CCTACCCTCTTATTTTCTTTGGATGGATTCAGGGCAGAATATTTACACACTTGGGGTGGACTTCTTCCTG
  TTATTAGCAAACTAAAAAAATGTGGAACATATACTAAAAACATGAGACCGGTATATCCAACAAAAACTTT
  CCCCAATCACTACAGCATTGTCACCGGATTGTATCCAGAATCTCATGGCATAATCGACAATAAAATGTAT
  GATCCCAAAATGAATGCTTCCTTTTCACTTAAAAGTAAAGAGAAATTTAATCCTGAGTGGTACAAAGGAG
  AACCAATTTGGGTCACAGCTAAGTATCAAGGCCTCAAGTCTGGCACATTTTTCTGGCCAGGATCAGATGT
  GGAAATTAACGGAATTTTCCCAGACATCTATAAAATGTATAATGGTTCAGTACCATTTGAAGAAAGGATT
  TTAGCTGTTCTTCAGTGGCTACAGCTTCCTAAAGATGAAAGACCACACTTTTACACTCTGTATTTAGAAG
  AACCAGATTCTTCAGGTCATTCATATGGACCAGTCAGCAGTGAAGTCATCAAAGCCTTGCAGAGGGTTGA
  TGGTATGGTTGGTATGCTGATGGATGGTCTGAAAGAGCTGAACTTGCACAGATGCCTGAACCTCATCCTT
  ATTTCAGATCATGGCATGGAACAAGGCAGTTGTAAGAAATACATATATCTGAATAAATATTTGGGGGATG
  TTAAAAATATTAAAGTTATCTATGGACCTGCAGCTCGATTGAGACCCTCTGATGTCCCAGATAAATACTA
  TTCATTTAACTATGAAGGCATTGCCCGAAATCTTTCTTGCCGGGAACCAAACCAGCACTTCAAACCTTAC
  CTGAAACATTTCTTACCTAAGCGTTTGCACTTTGCTAAGAGTGATAGAATTGAGCCCTTGACATTCTATT
  TGGACCCTCAGTGGCAACTTGCATTGAATCCCTCAGAAAGGAAATATTGTGGAAGTGGATTTCATGGCTC
  TGACAATGTATTTTCAAATATGCAAGCCCTCTTTGTTGGCTATGGACCTGGATTCAAGCATGGCATTGAG
  GCTGACACCTTTGAAAACATTGAAGTCTATAACTTAATGTGTGATTTACTGAATTTGACACCGGCTCCTA
  ATAACGGAACTCATGGAAGTCTTAACCACCTTCTAAAGAATCCTGTTTATACGCCAAAGCATCCCAAAGA
  AGTGCACCCCCTGGTACAGTGCCCCTTCACAAGAAACCCCAGAGATAACCTTGGCTGCTCATGTAACCCT
  TCGATTTTGCCGATTGAGGATTTTCAAACACAGTTCAATCTGACTGTGGCAGAAGAGAAGATTATTAAGC
  ATGAAACTTTACCCTATGGAAGACCTAGAGTTCTCCAGAAGGAAAACACCATCTGTCTTCTTTCCCAGCA
  CCAGTTTATGAGTGGATACAGCCAAGACATCTTAATGCCCCTTTGGACATCCTATACCGTGGACAGAAAT
  GACAGTTTCTCTACGGAAGACTTCTCCAACTGTCTGTACCAGGACTTTAGAATTCCTCTTAGTCCTGTCC
  ATAAATGTTCATTTTATAAAAATAACACCAAAGTGAGTTACGGGTTCCTCTCCCCACCACAACTAAATAA
  AAATTCAAGTGGAATATATTCTGAAGCTTTGCTTACTACAAATATAGTGCCAATGTACCAGAGTTTTCAA
  GTTATATGGCGCTACTTTCATGACACCCTACTGCGAAAGTATGCTGAAGAAAGAAATGGTGTCAATGTCG
  TCAGTGGTCCTGTGTTTGACTTTGATTATGATGGACGTTGTGATTCCTTAGAGAATCTGAGGCAAAAAAG
  AAGAGTCATCCGTAACCAAGAAATTTTGATTCCAACTCACTTCTTTATTGTGCTAACAAGCTGTAAAGAT
  ACATCTCAGACGCCTTTGCACTGTGAAAACCTAGACACCTTAGCTTTCATTTTGCCTCACAGGACTGATA
  ACAGCGAGAGCTGTGTGCATGGGAAGCATGACTCCTCATGGGTTGAAGAATTGTTAATGTTACACAGAGC
  ACGGATCACAGATGTTGAGCACATCACTGGACTCAGCTTCTATCAACAAAGAAAAGAGCCAGTTTCAGAC
  ATTTTAAAGTTGAAAACACATTTGCCAACCTTTAGCCAAGAAGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006208 unedited
  GGCTGGGATTTGTAAACGACTTATATAGGCGGCCGCGCATTCGGCACGAGGGCCACGATG
  GTAGCGCGACGGCTGCGCGGGGGGCGGGAGCCGCGGCGGCGAGGGCGGGCGCGCTCCCCG
  GGAGGGCCCGGCGGGGAACGGCCGCGATCGGGGCCGCAGCCACGCTGCCGAGGCGCCCGG
  GGACCCGCAGGCGGCCGCGTCCTTGCTGGCCCTTATGGACGTGGGGGAGGAGCCGCTGGA
  GAAGGCGGCGCGCGCCCGCACTGCCAAGGACCCCAACACCTATAAAGTACTCTCGCTGGT
  ATTGTCAGTATGTGTGTTAACAACAATACTTGGTTGTATATTTGGGTTGAAACCAAGCTG
  TGCCAAAGAAGTTAAAAGTTGCAAAGGTCGCTGTTTCGAGAGAACATTTGGGAACTGTCG
  CTGTGATGCTGCCTGTGTTGAGCTTGGAAACTGCTGTTTAGATTACCAGGAGACGTGCAT
  AGAACCAGAACATATATGGACTTGCAACAAATTCAGGTGTGGTGAGAAAAGGTTGACCAG
  AAGCCTCTGTGCCTGTTCAGATGACTGCAAGGACCAGGGCGACTGCTGCATCAACTACAG
  TTCTGTGTGTCAAGGTGAGAAAAGTTGGGTAGAAGAACCATGTGAGAGCATTAATGAGCC
  ACAGTGCCCAGCAGGGTTTGAAACGCCTCCTACCCTCTTATTTTCTTTGGATGGATTCAG
  GGCAGAATATTTACACACTTGGGGTGGACTTCTTCCTGTTATTAGCAAACTAAANAAATG
  TGGAACATATACTANNAACATGAGACCGGTATATCCAACAAAAACTTTCCCCAATCACTA
  CAGCATTGTCACCGGGATGTATCCAGAATCTCATGGCATAATCGACAATAAATGTATGAT
  CCCCAAATGAATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006208 unedited
  AGCTTTGNAACCGCGGCCGCAATCTANAAGTCGGTTTTTTTTTTTTTTTTTGATTACAAT
  GAAAAGAAATGAAACTCCAAGTTAAAAGATATCCAGAACCACTTTAAAATTCTCATAAAG
  ATAGTCACATAACATGGGCTACCCCAACCCTACTATAAGTCTACTGTTTGCCCAATGCCA
  TCAAGGTTTATTACCAATAGTAAGAAAGATTCAAATATCTGGTATCCAGACAATGTTCAC
  AGCTACTTCTCCTTTAAGAGAAGCAGAAAGGCAGTTTTAGGTGTGGTCTGTCTTTATTCC
  CCCGAACTTGATCAATGTATACAATGCTTACACATTCAAATATGTGCAAGATTCAACAGG
  AACACTCTCCACTGCTGTGCTGAGTACGCAAGTTTCCCTGAGATGTCCGCATCACCGAGG
  GCTCCGTTCTAACTCTGGTCGACAGTTTCTGAACAATGCAATAGTGTAGCTAGAGGACTA
  TATAAAATATAAGGTTCTCTCAAAAAGATTCATGGTGTTTGGGGATAAAAAACATATCAG
  TCTTCTTGGCTAAAGGTTGGCAAATGTGTTTTCAACTTTAAAATGTCTGAAACTGGCTCT
  TTTCTTTGTTGATAGAAGCTGAGTCCAGTGATGTGCTCAACATCTGTGATCCGTGCTCTG
  TGTAACATTAACAATTCTTCAACCCATGAGGAGTCATGCTTCCCATGCACACAGCTCTCG
  CTGTTATCAGTCCTGTGAGGCAAAATGAAAGCTAAGGTGTCTTAGGTTTTCACAGTGCAA
  AAGCGTCTGAGATGTATNCTTACAGCTTGTTAGCACATAAAGAAATGAGTTTGGATCAAA
  ATTCCTGGTTACGGATGACTCTNCTTTTTTGCTCAGATCTCTAAGGATCACACGTNCATC
  ATATCAAGTCAAACACAGGACACTGACGACATTGACACCATTTCTTTCTCANCTACTTTT
  TCCATAGGGTGTATGAAAGTACGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006208
• Insert Size: 3160 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006208.1, NP_006199.1
• RefSeq Size: 7442 bp
• RefSeq ORF: 2622 bp
• Locus ID: 5167
• Cytogenetics: 6q23.2
• Domains: SO, Endonuclease, Phosphodiest
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Pantothenate and CoA biosynthesis, Purine metabolism, Riboflavin metabolism, Starch and sucrose metabolism
• Gene Summary: 'This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]'