GRP94 (HSP90B1) (NM_003299) Human Untagged Clone

CAT#: SC108810

HSP90B1 (untagged)-Human heat shock protein 90kDa beta (Grp94), member 1 (HSP90B1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSP90B1
• Synonyms: ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108810 sequence for NM_003299 edited (data generated by NextGen Sequencing)
  ATGAGGGCCCTGTGGGTGCTGGGCCTCTGCTGCGTCCTGCTGACCTTCGGGTCGGTCAGA
  GCTGACGATGAAGTTGATGTGGATGGTACAGTAGAAGAGGATCTGGGTAAAAGTAGAGAA
  GGATCAAGGACGGATGATGAAGTAGTACAGAGAGAGGAAGAAGCTATTCAGTTGGATGGA
  TTAAATGCATCACAAATAAGAGAACTTAGAGAGAAGTCGGAAAAGTTTGCCTTCCAAGCC
  GAAGTTAACAGAATGATGAAACTTATCATCAATTCATTGTATAAAAATAAAGAGATTTTC
  CTGAGAGAACTGATTTCAAATGCTTCTGATGCTTTAGATAAGATAAGGCTAATATCACTG
  ACTGATGAAAATGCTCTTTCTGGAAATGAGGAACTAACAGTCAAAATTAAGTGTGATAAG
  GAGAAGAACCTGCTGCATGTCACAGACACCGGTGTAGGAATGACCAGAGAAGAGTTGGTT
  AAAAACCTTGGTACCATAGCCAAATCTGGGACAAGCGAGTTTTTAAACAAAATGACTGAA
  GCACAGGAAGATGGCCAGTCAACTTCTGAATTGATTGGCCAGTTTGGTGTCGGTTTCTAT
  TCCGCCTTCCTTGTAGCAGATAAGGTTATTGTCACTTCAAAACACAACAACGATACCCAG
  CACATCTGGGAGTCTGACTCCAATGAATTTTCTGTAATTGCTGACCCAAGAGGAAACACT
  CTAGGACGGGGAACGACAATTACCCTTGTCTTAAAAGAAGAAGCATCTGATTACCTTGAA
  TTGGATACAATTAAAAATCTCGTCAAAAAATATTCACAGTTCATAAACTTTCCTATTTAT
  GTATGGAGCAGCAAGACTGAAACTGTTGAGGAGCCCATGGAGGAAGAAGAAGCAGCCAAA
  GAAGAGAAAGAAGAATCTGATGATGAAGCTGCAGTAGAGGAAGAAGAAGAAGAAAAGAAA
  CCAAAGACTAAAAAAGTTGAAAAAACTGTCTGGGACTGGGAACTTATGAATGATATCAAA
  CCAATATGGCAGAGACCATCAAAAGAAGTAGAAGAAGATGAATACAAAGCTTTCTACAAA
  TCATTTTCAAAGGAAAGTGATGACCCCATGGCTTATATTCACTTTACTGCTGAAGGGGAA
  GTTACCTTCAAATCAATTTTATTTGTACCCACATCTGCTCCACGTGGTCTGTTTGACGAA
  TATGGATCTAAAAAGAGCGATTACATTAAGCTCTATGTGCGCCGTGTATTCATCACAGAC
  GACTTCCATGATATGATGCCTAAATACCTCAATTTTGTCAAGGGTGTGGTGGACTCAGAT
  GATCTCCCCTTGAATGTTTCCCGCGAGACTCTTCAGCAACATAAACTGCTTAAGGTGATT
  AGGAAGAAGCTTGTTCGTAAAACGCTGGACATGATCAAGAAGATTGCTGATGATAAATAC
  AATGATACTTTTTGGAAAGAATTTGGTACCAACATCAAGCTTGGTGTGATTGAAGACCAC
  TCGAATCGAACACGTCTTGCTAAACTTCTTAGGTTCCAGTCTTCTCATCATCCAACTGAC
  ATTACTAGCCTAGACCAGTATGTGGAAAGAATGAAGGAAAAACAAGACAAAATCTACTTC
  ATGGCTGGGTCCAGCAGAAAAGAGGCTGAATCTTCTCCATTTGTTGAGCGACTTCTGAAA
  AAGGGCTATGAAGTTATTTACCTCACAGAACCTGTGGATGAATACTGTATTCAGGCCCTT
  CCCGAATTTGATGGGAAGAGGTTCCAGAATGTTGCCAAGGAAGGAGTGAAGTTCGATGAA
  AGTGAGAAAACTAAGGAGAGTCGTGAAGCAGTTGAGAAAGAATTTGAGCCTCTGCTGAAT
  TGGATGAAAGATAAAGCCCTTAAGGACAAGATTGAAAAGGCTGTGGTGTCTCAGCGCCTG
  ACAGAATCTCCGTGTGCTTTGGTGGCCAGCCAGTACGGATGGTCTGGCAACATGGAGAGA
  ATCATGAAAGCACAAGCGTACCAAACGGGCAAGGACATCTCTACAAATTACTATGCGAGT
  CAGAAGAAAACATTTGAAATTAATCCCAGACACCCGCTGATCAGAGACATGCTTCGACGA
  ATTAAGGAAGATGAAGATGATAAAACAGTTTTGGATCTTGCTGTGGTTTTGTTTGAAACA
  GCAACGCTTCGGTCAGGGTATCTTTTACCAGACACTAAAGCATATGGAGATAGAATAGAA
  AGAATGCTTCGCCTCAGTTTGAACATTGACCCTGATGCAAAGGTGGAAGAAGAGCCTGAA
  GAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACACAGAGCAAGACGAAGATGAA
  GAAATGGATGTGGGAACAGATGAAGAAGAAGAAACAGCAAAGGAATCTACAGCTGAAAAA
  GATGAATTGTAA
  
  Clone variation with respect to NM_003299.1
  2277 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003299 unedited
  TCAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGCGGCT
  GGAGGTGTGAGGATCCGAACCCAGGGGTGGGGGGTGGAGGCGGCTCCTGCGATCGAAGGG
  GACTTGAGACTCACCGGCCGCACGCCATGAGGGCCCTGTGGGTGCTGGGCCTCTGCTGCG
  TCCTGCTGACCTTCGGGTCGGTCAGAGCTGACGATGAAGTTGATGTGGATGGTACAGTAG
  AAGAGGATCTGGGTAAAAGTAGAGAAGGATCAAGGACGGATGATGAAGTAGTACAGAGAG
  AGGAAGAAGCTATTCAGTTGGATGGATTAAATGCATCACAAATAAGAGAACTTAGAGAGA
  AGTCGGAAAAGTTTGCCTTCCAAGCCGAAGTTAACAGAATGATGAAACTTATCATCAATT
  CATTGTATAAAAATAAAGAGATTTTCCTGAGAGAACTGATTTCAAATGCTTCTGATGCTT
  TAGATAAGATAAGGCTAATATCACTGACTGATGAAAATGCTCTTTCTGGAAATGAGGAAC
  TAACAGTCAAAATTAAGTGTGATAAGGAGAAGAACCTGCTGCATGTCACAGACACCGGTG
  TAGGAATGACCAGAGAAGAGTTGGTTAAAAACCTTGGTACCATAGCCAAATCTGGGACAA
  GCGAGTTTTTAAACAAAATGACTGAAGCACAGGAAGATGGCCAGTCAACTTCTGAATTGA
  TTGGCCAGTTTGGGTGTCGGTTTCTATTCCGCCTTCCTTGTAGCAGATAAGGTTATTGTC
  ACTTCAAAAACACAACGATACCCAGACATCTGGGAGTCTGACTCCATGAATTNTCTGTAT
  TGCTGACCCAGAGGAACACTCTAGACGGGAACGNACATACCTTGTCTTAAAGAGAGCTCT
  GATACCTTGATNGGATACATAAACTCGCAAAATATCCAGTNATAACTTTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003299 unedited
  NANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTATTGGGGATCTTTTTTATTTTTATCCCCCTGACAGGTTTTTCCCTCAAGAAT
  AGCCCGTTAAATAGTCCAAATTTCCCTTCTTGGGGAATGTTAAAAAAAATTCACCTAAAA
  ACCCCCCTTTTTCCTGGGCCCCATAACCCCCCTTTTTACAGGGCAGGGGAAAAGGGGTTT
  AGGGGGCATCCAAAACAAGTTTTTCCCAAAAAAAAAGGGTGTAAATTTCACATTCCCTCC
  CCACCCGGGATCCAAAGGGGGGGGGTTTAATTTCCAATTCATTTTTTTCAGCGGGAGATC
  CCTTGGGGGGTTCTTCTTTTTCATCGGGCCCCCCATCCATTTTTTCATCTTCGTCTGGCT
  CGGGGGCTTCGGGGGGGGCTTCGGGGGGCTTTTCAGGTTCTTTTTCAGGCTTTTTTTCCA
  CCTTTGCATCGGGGGCAATGTTCAAACTGGGGGGAAGCATTCTTTCTTTTCTATCCCCAT
  ATGCTTTAGGGGCGGGAAAAAAATACCCTGCCCGAAGCGTTGCTGTTTCAAACAAAACCC
  CCGCAGGTTCCAAAACTGGTTTATCATCTTCACCTTCCTTAATTCGCCAAACCATGTTTT
  TGATCAGCGGGGGTCTGGGATTAATTTACAAGGGTTTTCTTCTGACTCCCATAGTAATTT
  GGAAAAAATTCCTTGCCCGTTTGACCCCCTGGGCCTTCAGGATTCTCTCCTGGGGCCGCA
  CACTCCGTCGGCGTGGCCCCACAGCCCCGGAAATTTTGGTCGGGCCTGAACCCCCCACCT
  TTTAATCTTGGCCTTAGGGT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003299
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003299.1, NP_003290.1
• RefSeq Size: 2780 bp
• RefSeq ORF: 2412 bp
• Locus ID: 7184
• Cytogenetics: 12q23.3
• Domains: HSP90, HATPase_c
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer
• Gene Summary: 'This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]'