BAF53A (ACTL6A) (NM_004301) Human Untagged Clone

CAT#: SC108889

ACTL6A (untagged)-Human actin-like 6A (ACTL6A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACTL6A
• Synonyms: ACTL6; Arp4; ARPN-BETA; BAF53A; INO80K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004301 edited
  GAATTCGGCACGAGGTGTGGCTGAGCTCCGGGGTGTGTGGACGCCGCTTTGTTGCCTGAG
  ATGAAGTTGGAGCCCTTGTTTTTGACATTGGATCCTATACTGTGAGAGCTGGTTATGCTG
  GTGAGGACTGCCCCAAGGTGGATTTTCCTACAGCTATTGGTATGGTGGTAGAAAGAGATG
  ACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAACAAGGCGGTCCCACCTACT
  ACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGGCCATTTCACCTCTAA
  AAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGGATCATACCTACAAAA
  TGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAGAGGCACCGTGGAATA
  CTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAACACTACAACATCCCTGCCT
  TCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAATGGTCGTTCTACTGGGCTGA
  TTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTCCACGATGGCTATGTCCTTC
  AACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTTTATTACTATGCAGTGCAGAGAAC
  TCTTCCAAGAAATGAATATTGAATTGGTTCCTCCATATATGATTGCAACAAAAGAAGCTG
  TTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAAGTTGCCTCAGGTTACGAGGT
  CTTGGCACAATTATATGTGTAATTGTGTTATCCAGGATTTTCAAGCTTCGGTACTTCAAG
  TGTCAGATTCAACTTATGATGAACAAGTGGCTGCACAGATGCCAACTGTTCATTATGAAT
  TCCCCAATGGCTACAATTGTGATTTTGGTGCAGAGCGGCTAAAGATTCCAGAAGGATTAT
  TTGACCCTTCCAATGTAAAGGGGTTATCAGGAAACACAATGTTAGGAGTCAGTCATGTTG
  TCACCACAAGTGTTGGGATGTGTGATATTGACATCAGACCAGGTCTCTATGGCAGTGTAA
  TAGTGGCAGGAGGAAACACACTAATACAGAGTTTTACTGACAGGTTGAATAGAGAGCTGT
  CTCAAAAAACTCCTCCAAGTATGCGGTTGAAATTGATTGCAAATAATACAACAGTGGAAC
  GGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTAGCCTCTTTGGGTACCTTTCAACAGA
  TGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGGAAGCAGTGTGTAGAAAGAAAATGCC
  CTTGAGAAAGAGTTCCCAAGCTTCTACCTTCCTTTTGTCACCTTACGTTTCATAGCTTTA
  GTATACTCAGGAAAAGAATGACCATCTTTTGTAGAATGTTTATACATTTTTGCATATTTC
  AATTTCCACTTAAATTTTTTAAAGCTTTAACTGGCTCTATAAATTAAGTTTGTGCTTTCC
  TTGAAATGCACTTATTCTTATTACAAGCATTTTATAATTTTGTATAAATGTCTATTTTCT
  CTAAATATTTTGCTTTCAGTAAAATGCTTTCCAACTCTGTTTAGTGTATTAATTACCAGT
  GGATTGGTAGAACTGCTTTTTATTGACTAGTAAAAGTTACTGCCTATGCTTTTTACCTTA
  GGCTTACAGAATTAAATAAAAATTAGCCATTCCAGAAATATAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004301 unedited
  CCTTCGCCATTTGTATACGATTTACTATAGGGCGGCCGCGATTCGGCACGAGGAGTCACT
  TCGCCAGTTAGCCCTTAGGGTAGGAGTCGCGCCGGCAGCAGCCATGAGCGGCGGCGTGTA
  CGGGGGAGATGAAGTTGGAGCCCTTGTTTTTGACATTGGATCCTATACTGTGAGAGCTGG
  TTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTACAGCTATTGGTATGGTGGTAGA
  AAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAACAAGGCGGTCC
  CACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGGCCATTTC
  ACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGGATCATAC
  CTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAGAGGCACC
  GTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAACACTACAACAT
  CCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAATGGTCGTTCTAC
  TGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTCCACGATGGCTA
  TGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTNTATTACTATGCAGTG
  CAGAGAACTCTTCCAAGAAATGAATATTTGAATCGGTTCCTCCCATATATGATTGCACAA
  AAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAGTTGCCTCAGGT
  TACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCCAGATTTTCAGCCTTCGTA
  CTCCAAGAGTCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004301 unedited
  TGTACCGCGGGCCGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTATTTCTGGAATGG
  CTAATTTTTATTTAATTCTGTAAGCCTAAGGTAAAAAGCATAGGCAGTAACTTTTACTAG
  TCAATAAAAAGCAGTTCTACCAATCCACTGGTAATTAATACACTAAACAGAGTTGGAAAG
  CATTTTACTGAAAGCAAAATATTTAGAGAAAATAGACATTTATACAAAATTATAAAATGC
  TTGTAATAAGAATAAGTGCATTTCAAGGAAAGCACAAACTTAATTTATAGAGCCAGTTAA
  AGCTTTAAAAAATTTAAGTGGAAATTGAAATATGCAAAAATGTATAAACATTCTACAAAA
  GATGGTCATTCTTTTCCTGAGTATACTAAAGCTATGAAACGTAAGGTGACAAAAGGAAGG
  TAGAAGCTTGGGAACTCTTTCTCAAGGGCATTTTCTTTCTACACACTGCTTCCCTCCTTC
  TTCATATTCTTGCTTGGAAATCCACATCTGTTGAAAGGTACCCAAAGAGGCTAGAATGGA
  GCCGCCAATCCATGAGCTAAACCTCCGTTCCACTGTTGTATTATTTGCAATCAATTTCAA
  CCGCATACTTGGAGGAGTTTTCTGAGACAGCTCTCTATTCAACCTGTCAGTAAAACTCTG
  TATTAGTGTGTTTCCTCCTGCCACTATTACACTGCCATAGAGACCTGGTCTGATGTCAAT
  ATCACACATCCCAACACTTGTGGTNGACACATGACTGACTCCTAACATGNNGTTCCTGAT
  ACCCCTTTACATTGGAAGGTNCAATAATCCCTTCTGAATCTTTTACCGCTCTGCACCAAA
  TCACAATTGTAGCCTTGGGGATTCCTAATGAACAGTTGCATCTGGCAGCCACTGTTATCA
  TAAGTGATCTGACCTTGAAGACCGAACTGAAAATCTGGATAACATTACCCTTATTGGGCC
  ACACTTGAACTGAGGACTTTCTTCCTTTCAGATGTGGAAACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004301
• Insert Size: 1930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004301.2, NP_004292.1
• RefSeq Size: 1879 bp
• RefSeq ORF: 1290 bp
• Locus ID: 86
• Cytogenetics: 3q26.33
• Domains: ACTIN
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (a), which is also referred to as hArpNbeta/Baf53. Isoform a is found mostly in the nucleus.